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README for OrthoSelect
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Test data
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The directory "project1" contains a test run using publicly available ESTs.
The following executable have to be in your path so that OrthoSelect can correctly work:
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blastall
hmmbuild
hmmsearch
genewise
ESTScan
muscle
wget (optional)
noisy (optional)
t_coffee
muscle
The following Environmental variables have to be set:
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WISECONFIGDIR
ESTSCANDIR
The following ESTScan matrices will be used (put them in the ESTSCANDIR)
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At.smat
Dm.smat
Dr.smat
Hs.smat
Mm.smat
Rn.smat
The archive includes these files:
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README
INSTALL
perl_scripts/auto_download.pl
perl_scripts/create_taxa_file.pl
perl_scripts/gene_selection.pl
db/
lib/
lib/Blaststuff.pm
lib/Iostuff.pm
options.txt
perl_scripts/
perl_scripts/post_processing.pl
perl_scripts/prepare_analysis.pl
perl_scripts/select_best_ortholog.pl
perl_scripts/start_blasto_nucleotide.pl
perl_scripts/start_filter_redundant_cluster.pl
perl_scripts/start_filter_redundant_single.pl
perl_scripts/start_orthology_assignment_cluster.pl
perl_scripts/start_orthology_assignment_single.pl
perl_scripts/stats.pl
est_libraries/
User_guide.pdf
The documentation is available as a pdf document:
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User_guide.pdf
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OrthoSelect: a protocol for selecting orthologous groups in phylogenomics
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