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MiniOne-DNA-Fingerprinting-MiniLab_APSI2019_LR-1

The DNA Fingerprinting MiniLab aims to teach students about gel electrophoresis and its application in determining parentage through DNA analysis. Participants will learn to use micropipettes, prepare agarose gels, and analyze DNA band patterns to identify familial relationships, specifically in the context of humpback whales. The lab aligns with educational standards for grades 4-12, emphasizing the importance of genetics in tracing family history.

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sanviparikh2
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16 views

MiniOne-DNA-Fingerprinting-MiniLab_APSI2019_LR-1

The DNA Fingerprinting MiniLab aims to teach students about gel electrophoresis and its application in determining parentage through DNA analysis. Participants will learn to use micropipettes, prepare agarose gels, and analyze DNA band patterns to identify familial relationships, specifically in the context of humpback whales. The lab aligns with educational standards for grades 4-12, emphasizing the importance of genetics in tracing family history.

Uploaded by

sanviparikh2
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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DNA Fingerprinting MiniLab

APSI 2019
Learning Objectives
Technical Objectives
• Correctly use an adjustable volume micropipette
• Prepare, load, and run an agarose gel
• Document gel electrophoresis results

Intellectual Objectives
• Understand the principles of gel electrophoresis and its importance in molecular
biology and genetics
• Analyze patterns of DNA bands run on an agarose gel and form a conclusion
about parentage
• Appreciate how genetics can be used to trace family history in people and
animals

2
NGSS Alignment for DNA Fingerprinting MiniLab
Phenomenon • How can we use DNA samples to determine paternity?
Grade Level • Basic genetics, electrophoresis, and data analysis: 4-12
• Student worksheet: grades 9-12
• With additional teacher support: grades 4-8

Disciplinary core ideas • LS1.A Structure and Function


• LS1.B Growth and Development of Organisms
• LS3.A Inheritance of Traits
• LS3.B Variation of Traits
• Interdependence of Science, Engineering and Technology

Science and engineering • Analyzing and interpreting data


practices

Crosscutting concepts • Recognizing and analyzing patterns in nature and


engineered systems
How does DNA fingerprinting work?

• Genomic DNA is cut with restriction enzymes, which


recognize specific base pair sequences.
• Because of differences in genomic sequence, individuals
will vary in the lengths of restriction fragments.
• Restriction fragments are separated using gel
electrophoresis giving a pattern unique to each individual.

4
DNA fingerprinting to establish parentage
• Every individual is genetically unique
• Half of your DNA comes from your mother and half from
your father
• DNA fingerprinting can be used to determine the identity of
a child’s parents
• The bands that are present in the child’s fingerprint and
missing from a known parent’s fingerprint must have come
from the other parent
• These “missing bands” can be compared to DNA
fingerprints of potential parents to find a match

5
The Scenario: which whale is Luna’s father?

• Scientists are using genetics to learn about the mating behaviors of


humpback whales
• By observing nursing patterns they know which whale is Luna’s mother
• Based on mating behavior, her father could be one of three males
• In this lab you will use DNA fingerprinting to determine which of these
three males is Luna’s father

6
Cast the agarose gel

*Use the six-well side*

7
Cast the agarose gel

8
Prepare the MiniOne electrophoresis
chamber
Prepare the MiniOne electrophoresis
chamber

10
Load your samples

11
Run your gel
Document your results
Example results

Which male is Luna’s father?


Example analysis

**All bands not from the mother must have


come from the father**

Step 1: determine which bands are present in


Luna’s fingerprint, but missing in her mother’s
fingerprint.
The Research Question:
Which male humpback is Luna’s father?
Our Claim:
YOUR ANSWER TO THE QUESTION

Our Evidence: Our Reasoning:

DATA EXPLAIN WHY THE DATA


YOU USED IS IMPORTANT
analysis and OR RELEVANT
interpretation to supporting your claim.
Example analysis

Step 2: determine which male’s


fingerprint contains ALL of the
“missing” bands.

Conclusion: Only Male A contains


both “missing” bands, therefore
Male A is Luna’s father.
Thank you for attending our workshop

Please use the comment form if there is any


feedback you would like to share.

Please contact us at [email protected]


for more information or to request a quote.

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