FORMULATION AND EVALUATION OF

POLYHERBAL HAIR CREAM

Mid-Term Presentation
Submitted to the Department of Pharmacognosy and
Phytochemistry

Presented By:- Guided By:-

Ms. Zoya Khan Ms. Apurva Tiwari
Ms. Aditi Chavhan M. Pharm.
Mr. Aditya Wanve Assistant Professor DBCOP,
(B.Pharm Final Year VIII Sem). Nagpur
Department Of Pharmacognosy
and Phytochemistry

DADASAHEB BALPANDE COLLEGE OF PHARMACY,

BESA, NAGPUR-440037
2023-2024
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 CONTENTS

1. Introduction

2. Literature Review

3. Aim, Objectives, and Need of Research

4. Plan of Work

5. References
 Introduction
• Mammals' hair displays diverse forms, hues, and growth patterns, serving functions
such as sensory stimulation, thermal insulation, and protection.
• Human hair goes beyond protection, embodying aesthetic, cultural, and attractiveness
ideals, often symbolizing youth, vigor, and health.
• While not a vital organ, hair significantly influences cosmetic appearance, impacting
how individuals perceive themselves.
• Characteristics like length, color, texture, and shape play crucial roles in this
perception, affecting self-esteem and confidence levels.
• The cultural significance of hair extends to various rituals and practices worldwide,
illustrating its profound impact on human societies throughout history.
Herbal formulations are those formulations with active ingredients made from plant or
plant parts such as leaves, flowers, fruits, etc. As they are know to have the least side effects
doesn’t mean that we should use them carelessly. As we are working on hair damage
repairing activity (anti-oxidant) we have chosen :

Sr. no. Common name Biological name Activity Image

1. Roselle, Jamaica Sorrel, Hibiscus Antibacterial activity,

Red Sorrel sabdariffa Antioxidant activity, Laxative
etc.

2. Curry Leaf Tree, Daun Murraya Antioxidative, Hepato-

Kari, Indian Curry Tree, koenigii protective activity,
Curry Bush Antimicrobial activity,
Antifungal activity etc.
What is a Polyherbal Hair Cream?

• Polyherbal cream is a type of cream that contains a

mixture of several herbs.
• This cream is commonly used in traditional medicine to
treat various skin conditions, such as eczema, psoriasis,
and acne.
• The combination of different herbs in polyherbal cream
can provide a wider range of benefits than a single herb
alone.
Additionally, some studies have suggested that
polyherbal cream may have anti-inflammatory and
antimicrobial properties.
 HAIR GROWTH CYCLE
Understanding the complex process of hair growth.
LITERATURE REVIEW

Sr.no. Author and title Journal and year Work done

This study aims to determine the hair growth activity of cream

Munifatul Lailiyah et al preparations of ethanol extract of hibiscus leaves with
1 Hair growth cream formulation Jurnal eduhealth 14 concentrations of 10%, 15%, and 20%. In addition, physical
from shoe flower leaf ethanol (02), 720-728, 2023 quality tests and stability tests of cream preparations were also
carried out. To make it easier to use, it needs to be made in
extract (Hibiscus rosa sinensis ). preparations that are practical and comfortable to use, such as in
the form of cream.

2. Juheini Amin et al.

Green tea [Camellia sinensis (L.) This study investigated the hair growth activity, safety and
Agriculture And
Kuntze] leaves extract and stability of green tea leaves ethanolic extract (GTE) and
Natural Resources 53
hibiscus leaves ethanolic extract (HTE) in topical
hibiscus (Hibiscus tilliaceus L.) (2), 139-147, 2019
microemulsion preparations activity of hair growth was
leaves extract as topical hair investigated using an in vivo method on rats; hair lengths were
growth promoter in measured on days 7, 14 and 21 and hair weight on day 21
microemulsion
Sr.no. Author and title Journal and year Work done

3. Sharma Pushpam et al International journal The medicinal herb bhringraj, often known as fake daisy, increases hair growth.
Formulation and evaluation of of Creative It's an ayurvedic substance that promotes hair growth . It improves blood
herbal hair pack. Research circulation to the scalp by encouraging and activating hair growth that has been
Thoughts lost due to a variety of factors, including dandruff. It also helps to prevent
(IJCRT) dandruff and irritation on the scalp, which can lead to hair loss.
[June ,2022]

Warintorn Ruksiriwanich et al
4. uava (Psidium guajava L.) Leaf MDPI In Plants Leaves of guava (Psidium guajava L.) have been used in Thai folk medicine without any
Extract as Bioactive Substances [2022, supporting evidence as a traditional herbal remedy for hair loss. Androgenetic alopecia
for AntiAndrogen and Antioxidant 11 ] ,3514 (AGA) is
Activities chronic hair loss caused by effects of androgens in those with a genetic predisposition,
resulting in
hair follicle miniaturization. Our objectives were to provide the mechanistic assessment of
guava
leaf extract on gene expressions related to the androgen pathway in well-known in vitro
models, hair follicle dermal papilla cells (HFDPC), and human prostate cancer cells (DU-
145), and to determine its bioactive constituents and antioxidant activities. Our study
reveals new perspectives on guava leaf extract’s anti-androgen properties. This extract
could be
developed as alternative products or therapeutic adjuvants for the treatment of AGA and
other
androgen-related disorders.
AIM:

• Formulation and Evaluation of Polyherbal Hair Cream.

OBJECTIVES:-

• Review of the literature

• Conduct a thorough analysis of the body of knowledge on each of the botanical ingredients or individual herbs suggested for the

polyherbal cream.

• To better understand the potential advantages, synergistic effects, and restrictions of polyherbal formulations, analyze earlier studies

on the subject.

• To evaluate the Antioxidant activity of the extract.

• To evaluate the nutritional content of the extract.

• Formulate and evaluate the hair cream for hair damage repair.
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NEED OF RESEARCH:
The study of polyherbal cream formulation and evaluation fills several vital needs and advances knowledge in several fields, including
medicine, science, and consumer health. These are some of the main justifications for performing such research:

 Traditional Knowledge Validation:

The use of herbal treatments for various health and skincare issues has a long history. The traditional knowledge of these plants is
supported and scientifically evaluated by research into polyherbal creams, perhaps revealing novel uses and advantages.

 Natural Approaches:
Polyherbal creams are in line with the rising popularity of natural practices. These formulations provide alternatives to synthetic or
chemical-based goods, appealing to people looking for more natural skincare and therapeutic solutions.

 Potentially Fewer Negative Effects:

When properly made, herbal extracts may provide possible therapeutic effects with fewer negative effects than some synthetic
chemicals. Research can aid in identifying secure and powerful combinations that reduce negative effects.
PLAN OF WORK
 Literature Review

 Selection of Herbs essential for hair growth.

 Authentication of the Drugs / Plants used

 Extraction- via Soxhlet Extraction.

 Evaluation of Extracts.

 Formulation of Nano Particles

 Characterization of Nanoparticles

 Formulation of Polyherbal hair cream.

 Evaluation of Polyherbal hair cream IMG:- Extraction Via Soxhlet Extraction.

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 Authentication of the Plants

M. koenigii Hibiscus sabdariffa

Authentication no.- 10716 Authentication no.- 10714
Extraction of the Drugs Via Soxhlet Extraction.

Soxhlet extraction of:- Soxhlet extraction of:-

Hibiscus sabdariffa Murraya koenigii
 Extracts of the Plants

Extract of:- Extract of:-

Murraya koenigii Hibiscus sabdariffa
150 g of crude drug yields 7 gram of 150 g of crude drug yields 10 gram of
extract extract
%yield of the extract = 7g*100/150 %yield of the extract = 10g*100/150
=4.6% =66.6%
Murraya koenigii :- 4.6% Hibiscus sabdariffa :- 66.6%
 Evaluation Of Extract
PRELIMINARY PHYTOCHEMICAL SCREENING of Murraya koenigii

Chemical Composition Ethanol Extract

 Alkaloid Present
Tannin Present
Anthraquinone Present
Glycoside Absent
Reducing Sugar Present
Saponin Absent
Flavonoids Present
Terpenoid Present
Cardiac glycoside Absent
 Evaluation Of Extract
PRELIMINARY PHYTOCHEMICAL SCREENING of Hibiscus sabdariffa

Chemical Composition Ethanol Extract

 Alkaloid Present
Tannin Present
Anthraquinone Present
Glycoside Absent
Reducing Sugar Present
Saponin Absent
Flavonoids Present
Terpenoid Present
Cardiac glycoside Absent
 Quantitative Evaluation Of Extract
Total Phenolic Content:-

0.5 ml of ethanolic solution of extract (1mg/ml) and 2.5 ml of 10% Folin-Ciocalteu’s reagent

Add 2.5 ml of 7.5 %NaHCO3, Allow to stand for 30 minutes

Absorbance was determined using a spectrophotometer at λmax = 765 nm.

The same procedure for Gallic acid at a concentration range of 100 - 1000 µg/ml,

the calibration curve was constructed as concentration v/s Absorbance.

 Quantitative Evaluation Of Extract
Total Flavonoid Content:-

Stock solution (1mg/ml) in ethanol

Different aliquots of 100-1000µg/ml were prepared in a 10ml volumetric

flask & 4 ml distilled water was added along with 0.3ml 5% NaNO 2.

After 5 min add 0.3 ml f 10% AlCl3 & further after 5-6 min add 2ml 1M

NaOH.

After the addition of NaOH, the solution turns red color. Incubate/keep at

a place for 30 minutes. Absorbance was measured at 510 nm

The calibration curve was plotted as concentration v/s Absorbance

 Formulation of Nanoparticles
Multiple approaches were adapted to make the best Formulation of Nanoparticles.

E.g.
 Solvent Evaporation
◦ Using Rota-Evaporator

◦ Using Magnetic Stirrer

Anti Solvent Precipitation

Salting Out.
 Anti Solvent Precipitation

Medicament + Soy Lecithin ( Equimolar )

Refluxed For 2 Hours

With 20 ml of Dichloromethane. This was unsuccessful as the

complete dryness was not
achieved,.

Concentrate It up to 5-10 ml. Store in an Ambered color bottle

Add 20 ml Of Hexane with continuous stirring. Pass through #100 mesh size

Filter it and collect in vacuum desiccator. Up to Dryness and Break into Mortar
 Solvent Evaporation
Using Rota-Evaporator
Soya-phosphatidylcholine + span 80 (90:10)+ Drug
dissolved in ethanol/alcohol.

Multilamellar lipid vesicles (MLV) are then Then the solution was put in a
sonicated at room temperature. round bottom flask.

Final traces are removed under vacuum. The These were then dissolved by shaking. Thin film was
deposited lipid film is hydrated with the then formed by keeping it in a rotator vacuum
appropriate buffer by rotation at 60rpm for 1 hr at evaporator at 40°
room temperature.
 Results of Rota Evaporator

Fig:- Rota Evaporator

Thin Film Formed

Again this method was not used because the desired results were not obtained and the results were unsatisfactory.
 Solvent Evaporation
Using Magnetic Stirrer
Preparation of Nanoparticles
a. Place the required amount of lecithin (1-4% w/v) into a small round bottom flask.

b. Add the ethanol (10-50% v/v) containing the drug to the flask.

c. Start the magnetic stirrer to solubilize the lecithin and the drug in the ethanol. Ensure thorough
mixing.

d. Cover the round bottom flask with its lid or an appropriate cover to prevent ethanol evaporation
during stirring.

e. Slowly add distilled water to the mixture while maintaining continuous stirring. This step is crucial to
obtain the colloidal suspension.
Fig:- Magnetic stirrer
f. Continue stirring for a sufficient time until the nanoparticle are formed. This usually takes around 30
minutes at room temperature.

g. After 30 minutes, stop the stirring and let the final nanoparticle suspension sit at room temperature.

This is the preferred procedure.

 Results
Trial and error methodology was employed to develop the described procedure for the preparation of colloidal suspensions,
using lecithin, ethanol, and distilled water.

For:- Murraya koenigii

Batches Soya lecithin Ethanol Drug Water Particle Size

1 7 ml 50 ml 10 g 20 ml 1018.6 nm

2 6.6 ml 50ml 10 g 20 ml 291.1 nm

3 1 ml 15 ml 1g 10 ml 224.4 nm
 Results
Trial and error methodology was employed to develop the described procedure for the preparation of colloidal suspensions,
using lecithin, ethanol, and distilled water.

For:- Hibiscus sabdariffa

Batches Soya lecithin Ethanol Drug Water Particle Size

1 1 ml 15 ml 1g 10 ml 491.6 nm

2 6.6 ml 50ml 10 g 20 ml 328.6 nm

3 1 ml 15 ml 1g 10 ml 252.9 nm
 Results for Flavonoid Content for
Hibiscus sabdariffa

Calculation.:- Using Formula TFC(Total Phenolic Content)=

Using the equation
0.4247
µ of QE /mg of extract.
Therefore the total flavonoid content
observed was 5.33/ml.

0.80 mg/ml
 Results Flavonoid Content for
Murraya koenigii

Calculation.:- Using Formula TFC(Total Phenolic Content)=

Using the equation

µg of QE /mg of extract.
Therefore the total flavonoid content
observed was 5.55. QE /mg of extract
0.83 mg/ml
Characterization of Nanoparticles
1. SEM
2. DSC
3. ZETA POTENTIAL
4. FTIR
5. NUTRITIONAL EVALUATION
THESE ARE SENT FOR THE EVALUATION RESULTS ARE PENDING.
 REFERENCES:-

1. Prashant Chavhan et al FORMULATION AND EVALUATION OF POLYHERBAL CREAM International

Journal of Current Pharmaceutical Research ISSN- 0975-7066 Vol 12, Issue 4, 2020
2. MV Vishvanathan, PM Unnikrishnan, Kalsuko Komatsu, Hirotoshi Fushimi. A brief introduction to Ayurvedic
system of medicine and some of its problems. Indian J Traditional Knowledge 2003; 2:159-69.
3. Nilofar Khan, Prajwal Jadhav et al Formulation and evaluation of polyherbal hair gel formulation. Journal of
Pharmacognosy and Phytochemistry 2022; 11(1): 32-36 https://doi.org/10.22271/phyto.2022.v11.i1a.14318
4. SK Uddandu Saheb, Aduri Prakash Reddy, K Rajitha, B Sravani, B Vanitha. Formulation and evaluation of
cream from naturally containing plant extract. World J Pharm Pharm Sci 2018;7:851-61.
5. .K Gopalasatheeskumar. A significant role of the soxhlet extraction process in pharmaceutical research.
Mintage J Pharm Med Sci 2018;7:43-7
6. Esposito E, Menegatti E, Cortesi R (2004) Ethosomes and liposomes as topical vehicles for azelaic acid: a pre-
formulation study. J Cosmet Sci 55:253–264
7. Pathana IB, Jawarea BP, Shelkeb S, Ambekarc W (2017) Curcumin loaded ethosomes for transdermal
application: formulation, optimization, in-vitro and in-vivo study. J Drug Deliv Sci Technol 44:49–57
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