SEPARATION OF INSOLUBLE

PRODUCTS

FILTRATION

NUR HIDAYAH ZAINAN

UNIVERSITI TEKNOLOGI MALAY
 OVERVIEW
Overview
 An insoluble substance is a substance (particle) that will not dissolve in a solvent even
after mixing (The main insoluble considered are whole cells, cell debris, and particles such
as inclusion bodies)

Filtration

Inclusion bodies are aggregates of specific types of protein found in neurons, a number of tissue cells
including red blood cells, bacteria, viruses, and plants
 LEARNING OUTCOME

 Understanding, defining and knowing the application of the filtration process

 Explain the mechanism of the filtration process.
 Describe the type and function of the filter medium
 Differentiate between constant pressure cake and constant rate cake
 Investigating methods for increasing the efficiency of the filtration process
 Differentiate the mode of operation for the filtration process
 Introduction
• Filtration, the process in which solid
particles in a liquid or gaseous fluid are
removed by the use of a porous medium
that permits the fluid to pass through but
retains the solid particles.
• The porous medium which allows the
liquid to go through while retaining the
solids is called the filter.
• The retained solid is called "the residue" or
"the cake".
• The clarified liquid is called "the effluent" or
the "filtrate"

https://youtu.be/WSdGxQBnhZU
 Application of Filtration in Bioprocessing
 Recovery of crystalline solids

 Recovery of cells from fermentation medium

 Clarification of liquids and gases

 Sterilization of liquids- https://www.youtube.com/watch?v=vcxy6FpfSuw

The term clarification is applied when the solid content in the feed does not exceed 1 wt %. In a clarification process, the
filtrate is the primary product.
 Types of Filtration

If recovery of solids
from the high solid • Liquid flows parallel to the filtration
content slurry is medium
desired, the process • Used for membrane filtration
called cake filtration

• Solid content does not exceed 1 %

• Filtrate is the primary products
 Mechanism of Filtration

Surface Filtration
• Particles are retained by a screening action and held
on the external surface of the filter
• Particles are not allowed to enter the filtration
medium
• Pore size is precisely defined
• Only particles with diameter below pore size –
permeate the filter
• Cake filtration & cross-flow filtration are based on
surface filtration
 Mechanism of Filtration

Depth Filtration
• Particles are allowed to penetrate pores and pore
networks present in the filtration medium
• Do not have a precise pore size – not absolute
• Particles with wider range in size – can permeate thru
the filter
• Larger components – trapped on the filter surface
• Smaller particles – random entrapment & adsorption
of matters occurs within the media structure
 Mechanism of Filtration
Depth Filtration

• Cheaper
• Particles retained within the filter by three mechanism:
• direct interception
• inertial impaction
• diffusional interception.
 Direct Interception
• The particle enters the pores or pore networks within the filtration
medium - gets trapped where the pore diameter becomes equal to the
particle diameter

Pore diameter=particle diameter Particles will be trapped

• Particles that have diameters smaller than the pore diameter get
trapped when pores are already constricted by collected particles.

Particle diameter < Pore diameter but trapped

 Inertial Impaction
• Particles being carried by fluids possess momentum on their mass
and velocity

• Pores in most filtration media are tortuous.

• The flow of fluids through these pores is usually laminar in nature

based on their small diameter.

• At tortuous sections - the fluid streamlines follows the curves while

particles due to their inertia continue to move straight as a result hit
the filter medium, lose their momentum, and are retained.

• This collection mechanism is referred to as inertial impaction .

Tortuous=full of twists and turns

 Diffusional Interception

• Diffusional interception is more relevant to the filtration of the gas.

• The gas molecules, due to their random motion continually bombard

suspended particles, particularly those smaller than 0.3 μm in
diameter.

• The suspended particles deviate from their streamlines and impact the
filter medium.

• Once this happens, the particles lose their momentum and are retained.

• Most clarification processes rely on depth filtration.

 Filter Medium
• The function of the filter medium is to act as an impermeable barrier for particulate matter.

• At the beginning of a cake filtration process, the role of the filter medium is to act as a barrier. However, once the cake formation
commences, the cake becomes the main particle-retaining barrier and the role of the filter medium is mainly as a support for the cake.

• The filter medium should have;

1. sufficient mechanical strength
2. resistant to the corrosive action of fluids being processed,
3. and offer low resistance to the flow of the filtrate.

• Commonly used filter media are:

• Filter paper
• Woven material
• Non-woven fiber pads
• Sintered and perforated glass
• Sintered and perforated metal
• Ceramics
• Synthetic membranes
IMPERMEABLE: NOT ALLOWING FLUID TO PASS THROUGH.
 Driving Force
• Filtration is driven by applying a pressure drop across the filter medium.

• The driving force can be applied by:

1. pressuring the feed side (positive pressure filtration or simply pressure filtration)
2. by creating a vacuum in the filtrate side (vacuum filtration)

• In the industry, both pressure and vacuum filtration are used.

• Pressure filtration can be  Vacuum filtration is commonly

driven by pressurizing the used in the laboratory.
feed using  Vacuum can be generated using
1. Compressed air a water jet injector or a
pressurization vacuum pump.
2. Maintaining a hydrostatic  Vacuum filtration is preferred
liquid head on the feed from a safety point of view
side.  Substances that form foam
3. The feed can also be cannot be filtered by vacuum
pressurized with a suitable filtration.
pump.  https://youtu.be/uxtXIvW4g
5k
 Constant Pressure Cake Filtration
• Constant pressure filtration refers to a filtration process where the driving force(i.e. the pressure
drop across the filter medium) is kept constant.

• If we consider the filtration of a newtonian liquid, which is free from particles, the flow of liquid through
the filter can be explained by darcy’s law.

• = VOLUMETRIC FILTRATION RATE (m3/s)

• = DARCY’S LAW PERMEABILITY (m2)
• = AREA OF FILTER MEDIUM (m2)
• = PRESSURE DROP ACROSS THE FILTER MEDIUM (Pa)
• = VISCOSITY (kg/m s)
• = THICKNESS OF THE FILTER MEDIUM (m)
 Constant Pressure Cake Filtration
• The permeability and thickness of a filter can be combined into a medium resistance term and
equation can be written as.

• = Media resistance (/m)

• The filtration rate can be expressed in terms of the volume of filtrate collected as shown below:

• = Cumulative filtrate volume (m3)

• The value can be determined by plotting a graph v(t) versus t
𝑄 = volumetric filtration rate (m3/s)
𝑘 = Darcy’s law permeability (m2)
𝐴 = Area of filter medium (m2)
∆𝑃 = Pressure drop across the filter medium (Pa)
𝜇 = viscosity (kg/m s)
𝑙 = thickness of the filter medium (m)
Constant Rate Cake
y=0.5t + 5

-next filtration will be carried out at twice the

filtration rate =2 x Q
-slope = 22=4 times the equation
-intercept= 2 times the equation

y=2t + 10 =2 (60 min) + 10 = 130 kPa

 Improvement of Filtration Efficiency

• Efficiency of cake filtration depends on achieving high

cake accumulation on the filter medium
• However, the filtration rate declines with cake
accumulation due to an increase in cake resistance

How to
overcom
e ???
• Feed pre-treatment
• Filter aid
 Improvement of Filtration Efficiency

Feed pre-treatment

• The feed can be treated by physical method (ex: heating) or by the addition of chemicals (coagulants/flocculants) to
obtain porous cake with low specific cake resistance.

• Drawback – thermolabile substance cannot be heated, addition of chemical not possible in some applications
 Improvement of Filtration Efficiency
Filter-aids
• Filter aids are substances that are mixed with feed for creating very porous cakes
• Filter aids are granular solids that are used in combination with filtration to enhance the rate of filtration
• This increases the filtration rate very significantly
• The filter aids can later be removed from the dried and powdered cake by suitable separation technique-sieving
• Rarely used when the cake is the product of interest
• Certain deformable substances present in the feed can block the pores within the filtration medium. When such substances are being filtered, it might be a good idea to
precoat the medium with a layer of filter aid.
 Factors influencing filtration
Properties of solid
Properties of solid Properties of liquids Objectives Temperature
in slurry
• Particle shape, • Density • Rate of formation • Whether the solid • Temperature of
size and charge • Viscosity of filter cake or liquid or both suspension
• Density • Corrosiveness especially in early are to be collected
• Particle size stage of filtration
distribution
• Rigidity or
compressibility of
solid under
pressure
• Tendency of
particle to
flocculate or
adhere together
 Mode of Operation

• Filtration can be carried out in different ways;

1. Filters can be operated as a batch, where the cake is removed after a run
2. Continuous, where the cake is removed continuously.
3. Slurry concentration by delayed cake filtration
4. Slurry concentration by cross-flow filtration
5. Cake washing
 Cake accumulation and removal in batch mode.
• The common mode for small-scale cake filtration.

• A batch of feed is pumped into the filter unit and filtration is carried out either at a constant rate or at
constant pressure.

• The process is terminated when the filtration rate gets unacceptably low, or when the pressure
required gets too high, or when the filtration device is filled with the filter cake.

• The cake is then removed from the device by scraping it off the filter medium. Often this requires the
dismantling of the filtration unit.

• The filter medium is usually then cleaned and made ready for the next batch.

• Examples of filtration devices operated in this mode include:

• Funnel filter
• Filter press
• Leaf pressure filter
• Vacuum leaf filter
 Continuous cake accumulation and removal.
• A batch process may not be suitable for large-scale cake filtration.
• Continuous filters which allow simultaneous cake accumulation and removal are usually used for
large-scale processes.
• Examples of such:
• Horizontal continuous filter
• Rotary drum filter
• The filter consists of concentric vertical disks mounted on a horizontal rotating shaft. The filter
operates on the same principle as the vacuum rotary drum filter. Each disk in hollow and
covered with a filter cloth and is partly submerged in the slurry.

https://www.youtube.com/watch?v=pggFRWM_Zlk
 Slurry concentration by delayed cake filtration

• The objective of the filtration process is to thicken the slurry, and the build-up of cake on
the filter medium is avoided.

• Slurry thickening can be achieved by controlling the thickness of the cake layer thereby
keeping the particulate matter in a suspended form on the feed side.

• This can be achieved by incorporating mechanical devices such as moving blades, which
continuously scrape off the cake from the filter surface.

• With the moving blade arrangement, the thickness of the cake is limited by the clearance
between the filter medium and the blade.

• This type of filtration can be carried out until the solid content on the feed side reaches a
critical level beyond which the slurry does not flow.
 Slurry concentration by cross-flow filtration
• An alternative way by which the build-up of cake
on the filter can be discouraged is by using a
cross-flow mode of operation.

• This is achieved by maintaining a very high

velocity of feed flow parallel to the surface of the
filter medium.

• Typical cross-flow rates may be 10 - 20 times the

filtration rate.

• However, cross-flow filtration cannot be used for

obtaining very thick slurry since the energy
required for maintaining the cross-flow velocity
becomes prohibitively high.
 Cake washing
• After its formation, the cake may contain a significant amount
of entrapped liquid.
• When the liquid is the product of interest, this entrapment
represents a loss of yield.
• When the cake is itself the product, the entrapped liquid
represents the presence of impurity.
• The entrapped liquid can be removed by cake washing.
• The washing liquid should not be a "new impurity".
• Its presence should either be acceptable in the final product
(i.e. either the cake or the filtrate), or it should be easily
"removable".