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Fed Batch Culture

It conation and encloses the detailed notes on Fed-Batch culture and of catabolic repression and substrate inhibitors too

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50 views16 pages

Fed Batch Culture

It conation and encloses the detailed notes on Fed-Batch culture and of catabolic repression and substrate inhibitors too

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PGMBslides
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© © All Rights Reserved
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SRI PARAMAKALYANI COLLEGE,

Reaacredited with B grade with a CGPA of 2.71 in the second cycle of NAAC
affiliated to manomanium sundaranar university, tirunelveli.
ALWARKURICHI-627412.
Post graduate & Research Centre – Department of Microbiology
(government aided)
ACADEMIC YEAR 2021-2022
I SEM CORE: MICROBIAL PHYSIOLOGY AND METABOLISM (ZMBM13)

UNIT-5
FED BATCH CULTURES

G.S.AMRISH VARSHAN SUBMITTED TO


REG NO:20211232516102
GUIDE:DR.S.VISWANATHAN
I M.SC MICROBIOLOGY ASSISTANT
PROFFESSOR&HEAD
ASSIGNED ON: 08/12/2021 SPKC -
ALWARKURUCHI
SYNOPSIS
• Introduction to fed batch culture
• Catabolic repression
• E.coli concept
• Substrate Inhibitor
• Need of Fed-Batch culture
• Advantages of Fed-Batch culture
What is Fed-Batch culture
• In general batch culture is a technique were the bacteria is grown in a large
scale to produce products
• In the batch culture there will be no interaction from the environment until the
product form
• In batch culture the nutrient is added only once and final harvest only takes
place
• But in fed batch culture we add nutrients before the start of the process as
well as during the process of cell culture
Catabolic Repression
• Catabolism Catabolites
• Breakdown of complex compounds into simpler compounds is called
catabolism the simpler compounds is called catabolites
• Repression means (or) is to control the production of some thing
• So catabolic repression is to control the production of some enzyme
E.Coli
E.coli • In the first case E.coli growing in a glucose containing medium it produces the enzyme
which breakdown the glucose
Glucose

• In the second case E.coli growing in a medium containing both glucose and fructose but
E.Coli
E.Coli it prefers to take glucose and the enzyme responsible for breakdown of glucose is produced
Glucose
fructose

• In the third case only lactose is present and E.coli produce the enzyme which is responsible
E.Coli
Fructose of breakdown of lactose
• So as we seen the example of E.coli in the absence of glucose only the lactose
digesting enzymes are produced

• So we clearly seen that the catabolites of glucose are preventing the formation of
lactose digestive enzyme

• This phenomenon is known as Catabolite repression.


Substrate Inhibition
• It is a phenomenon in which substrate itself is going to inhibit the reaction
• The increasing concentration of substrate beyond a optimum level will
inhibit the reaction
• Vmax at which the velocity of the reaction is maximum and after that
concentration of substrate beyond the optimum level there will be no
binding and it will be zero
• In substrate inhibition the inhibitors and the substrate have similar site of binding

• Only the difference is, the substrate binding site have more affinity towards the
substrate and site of inhibitors have low affinity towards the substrate

• The substrate will bind in the inhibitor site when the concentration of the
substrate is beyond the optimum level

• This phenomenon is known as substrate inhibition


Needs of Fed-Batch culture
• The main need is to overcome the Catabolic repression and the Substrate
inhibition
• It can be achieved by preventing the excess amount of nutrients in the
bioreactor
• So that in fed culture we only add nutrients when the bacterial cell needs it
• To also overcome substrate inhibition, by only providing sufficient
amount of substrate
Advantages of Fed-Batch culture
• It helps us to get high cell density ,the cell growth will decreases if they
face depletion of nutrients (or) lack of nutrient
• In Fed-Batch culture we periodically add nutrients when there is nutrients
deployments, so that the cell will start to proliferate more
• It enhance the growth of the cell which leads to more amount of
biomasses and products
• Useful when the products or biomass yields high at low substrate
SKILL GAINED THROUGH SEMINAR
• COMMUNICATION SKILL
• GAINED MORE SUBJECT KNOWLEDGE
• INCREASING OF THE CONFIDENCE LEVEL
• PERSONAL SKILL
• SUBJECT PLAN
REFERENCE

• Youtube
• Prescot
• https://en.wikipedia.org/wiki/Fed-batch_culture-Wikipedia
• https://www.slideshare.net/RengeshBalakrishnan/batch-fedbatch-continuous-cultivation-57209876
• Slideshare
THANK YOU
The Chairman.
The secretory.
Management committee.
The principle, Sri paramakalyani college.
The head-Department of microbiology.
The staff members- Department of microbiology.

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