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Created by Mendoza, Paul Joseph For Experiment 1 of BIO152-1L

The document provides instructions for preparing an onion root tip for observation of cell division under a microscope. The steps include: 1) Collecting freshly harvested onion root tips and placing them in a microfuge tube with hydrochloric acid, then heating the tube in a water bath. 2) Draining the acid and staining the root tip with Feulgen stain. 3) Cutting the stained root tip and mounting it on a microscope slide, then covering it with a cover slip. 4) Viewing the prepared slide under a microscope and focusing on the cells.

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PaulMendoza
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64 views1 page

Created by Mendoza, Paul Joseph For Experiment 1 of BIO152-1L

The document provides instructions for preparing an onion root tip for observation of cell division under a microscope. The steps include: 1) Collecting freshly harvested onion root tips and placing them in a microfuge tube with hydrochloric acid, then heating the tube in a water bath. 2) Draining the acid and staining the root tip with Feulgen stain. 3) Cutting the stained root tip and mounting it on a microscope slide, then covering it with a cover slip. 4) Viewing the prepared slide under a microscope and focusing on the cells.

Uploaded by

PaulMendoza
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Onion Root Tip (Preparation for Observation

of Cell Division
Using a dropper, collect a fair
Then collecting the root tips using a amount of 1 N HCl and put at least
Getting first a freshly harvest onion Keep the microfuge tube afloat
forceps and then placing the 2-3 drops of it inside the microfuge
and cutting the root tip using a inside the water bath for 12 to 15
acquired root tips to a microfuge tube with the root tip inside and
scissor. minutes under 60 degrees Celsius.
tube and fully sealing the tube. placing the tube on a floater then
putting it in the water bath.

Draining of HCl in the tube using a Draining of the Feulgen stain using Razor blade is taken and cutting the
Pasteur pipette and cleaning the a pipette and washing the unstained portion of the root tip is Then the root tip is then pressed
specimen with distilled water and specimen using distilled water. cut and then discarded. After that using the backside of the probe.
drain it again using the pipette. Sucking the water out using a getting a cover slip to cover the Making sure that the root tip is
Then putting at least 2-3 drops of pipette and getting the root tip specimen that is in the microscopic flattened and spread with at least a
Feulgen stain and intubating it for using forceps and placing it on the slide. Using a dissection probe to diameter of 0.5-1 cm.
at least 12 to 15 minutes. microscopic slide. put the cover slip.

Putting the slide in the microscope


stage. To get a clear result, you
have to adjust the microscope to
Created by Mendoza, Paul Joseph for
focus on the cell to get a clear
picture. Experiment 1 of BIO152-1L

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