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Microbial Growth Kinetics Analysis

The document discusses the Monod equation, an unstructured and nonsegregation model used to model microbial growth kinetics under certain assumptions. The Monod equation models specific growth rate as a function of substrate concentration using Michaelis-Menten saturation kinetics. It assumes that growth is limited by a single substrate and that changes in other nutrients do not affect growth rate. The specific growth rate is calculated based on the maximum specific growth rate, saturation constant, and substrate concentration.

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Dewi Wahyuningtyas
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209 views4 pages

Microbial Growth Kinetics Analysis

The document discusses the Monod equation, an unstructured and nonsegregation model used to model microbial growth kinetics under certain assumptions. The Monod equation models specific growth rate as a function of substrate concentration using Michaelis-Menten saturation kinetics. It assumes that growth is limited by a single substrate and that changes in other nutrients do not affect growth rate. The specific growth rate is calculated based on the maximum specific growth rate, saturation constant, and substrate concentration.

Uploaded by

Dewi Wahyuningtyas
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPT, PDF, TXT or read online on Scribd
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Quantifying Growth Kinetics

Monod equation: unstructured and nonsegregation model


Unstructured model: assuming fixed cell composition.

Applicable to balanced-growth condition:

- exponential growth phase in batch culture

- single-stage, steady state continuous culture

- cell response is fast compared to external changes

- the magnitude of the external changes is not too large


(e.g. 10%-20% variation from initial conditions).

Nonsegregation model: assuming all cells are the same


in the culture.
Quantifying Growth Kinetics
Monod equation: unstructured and
nonsegregation model

Assumption:
- a single enzyme system with Michaelis-Menten kinetics
is responsible for uptake of substrate S, and the amount
of that enzyme or its activity is sufficient low to be
growth-rate limiting.
- the relationship of specific growth rate to substrate
concentration assumes the form of saturation kinetics.
- a single chemical species is growth-rate limiting while
changes in other nutrient concentrations have no effect.
Monod type saturation growth kinetics
Quantifying Growth Kinetics
Monod equation: when applied to cellular
systems, the gross specific growth rate g
(1/time)
m S
g
KS S
m is the maximum specific growth rate (1/time).
Ks is the saturation constant or half-velocity constant (g/l)
when the substrate concentration S>> Ks
(exponential growth phase),
g m

When endogenous metabolism is unimportant, g net

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