Scribd
Upload
610 views

Action Potential

this presentation helps in learning the processes involved in genesis of action potential. It gives the ionic basis of action potential in a nerve

Uploaded by

nirilib
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
610 views

Action Potential

this presentation helps in learning the processes involved in genesis of action potential. It gives the ionic basis of action potential in a nerve

Uploaded by

nirilib
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 27

ACTION POTENTIAL

Dr. Niranjan Murthy


HL
Asst Prof of
Physiology
0
mV
-80 mV
+ + +
+
+
- - - +
-
+
-

- +

-80 mV
[K+] = 2.5
[Na+] = 125
[Cl-] = 130
A-

+ + +
+
+
- - - +
-
+
-

- +

-
[K+] = 135
[Na+] = 7
[Cl-] = 11
-80 mV
A-
Resting membrane potential
• Difference in the electrical potential
between inside and outside of cell at
rest
• RMP of excitable tissues:
(i) Skeletal muscle: -90 mv
(ii) Smooth muscle: -40 to -60 mv
(iii) Cardiac muscle: -70 mv
(iv) Nerve fiber: -70 to -90 mv
Distribution of ions across cell
membrane

Values in mmol/L
Ionic channels of concern
• Leaky
cationic &
anionic
channels
• Voltage
gated Na+
channel
• Voltage
gated K+
Donnan Effect
Equilibrium potential of-
sodium = +61 mv
Potassium = -94 mv
Goldman-Hodgkin-Katz
Equation
Measurement of membrane
potential
Action potential
• A brief & propagative change in the
membrane potential after excitation
Phases of Action Potential
1. Resting phase: Polarized state; -70 mv
2. Depolarization phase: membrane
becomes highly permeable to Na+
ions; membrane potential shoots
above zero
3. Repolarization phase: Na+ channels
close & K+ channels open; potential
falls back to resting levels
4. After depolarization phase: final 1/3rd
of repolarization; slow; 4 msec
5. After hyperpolarization phase: 1-2 mv
hyperpolarization; 35-40 msec
Other terminologies
• Latent period

• Stimulus artifact

• Threshold or firing level


Ionic basis of AP
1. Depolarization phase: It is due to
opening of voltage-gated Na+
channels and pouring in of Na+ ions
2. Repolarization phase: due to closure
of Na+ channels and opening of
voltage-gated K+ channels & efflux
of K+ ions
3. After depolarization: due to slow
efflux of K+ ions
4. After hyperpolarization: due to
delayed closure of voltage-gated K+
Voltage gated channels
Changes in Na+ & K+
conductance during an AP
Cathode ray oscilloscope
Voltage clamp techniques
Biphasic Action Potential
Compound Action Potential
You can also do something like
this. Believe in yourselves

You might also like