Biological Microscope

MB-135/MB-136
User Manual

This manual is for biological microscope Model MB-135/MB-136.To ensure the safety, obtain optimum performance and
to familiarize yourself fully with the use of this microscope, it is recommended strongly that you study this manual
thoroughly before operating the microscope.
CONTENTS MB-135/MB-136

CONTENTS .......................................................................................................... 1

USER NOTICE ..................................................................................................... 2

1. Components Name ............................................................................................ 3

2．Assembly......................................................................................................... 4

2.1 Assembly Diagram .................................................................................... 4

2.2 Assembly procedure.................................................................................. 5

3. Adjustment and Operation ................................................................................ 9

3.1 Adjustment set diagram ............................................................................ 9

3.2 Adjustment and Operation ...................................................................... 11

4．Technical Specifications ............................................................................... 15

5．Outfit ............................................................................................................. 17

6．Troubleshooting Guide ................................................................................. 19

1
USER NOTICE MB-135/MB-136

1. Safety Note
1. Open the box carefully to avoid the accessories, like lens, dropping to ground or being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is flat, horizontal and firm enough.

3. When moving the microscope, carefully carry it with the handle and the base.
4. Make sure the instrument is earthed, toavoid lighting strike.

5. For safety, be sure the main switch is in “O” (off) state and cut off the power supply before replacing
the bulb or the fuse. If you replace the bulb during use or right after use, allow the lamp bulb and
the lamp house to cool completely before touching.

(Designated bulb：1W S-LED or 6V20W Halogen Lamp)

6. Check the input voltage: be sure the input voltage which is signed in the back of the microscope is
consistent with the power supply voltage, or it will bring a serious damage to the instrument.

7. Always use the power cord provided by Novel.

8. The electrical equipment of the microscope should be discard as electronic waste.

II、Maintenance and Care

1. All the lenses have been adjusted properly; do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble
them carelessly by yourself.
3. Keep the instrument clean, and do not pollute the optical element when wiping away the dust on
the instrument.
4. The contaminations on the prism, like fingerprints and oil smudges, could be gently wiped with
a piece of soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether.
(Note that the alcohol and ether are highly flammable, do keep them away from the fire or
potential sources of electrical sparks, and use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the glass
components. To clean them, use a lint-free, softcloth slightly moistened with a diluted neutral
detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe away
the splash.
7. Do not disassemble any parts of the microscope, as this will affect the function or reduce the
performance of the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered with
a dust cover. Make sure the lamp socket is cool before covering the microscope.

2
 1. Components Name MB-135/MB-136

Eyepiece
Binocular viewing head

Nosepiece

Objective

Condenser

Stage

Illumination

Main body

3
2．Assembly MB-135/MB-136

2.1 Assembly Diagram

The following figure shows the installation sequence of the components. The number in the figure
shows the assembly steps.

 Before installing, be sure every components is clean, do not score any parts or glass surface.

 Keep well with hexagon wrench provided. When changing the components, you will need it again.

10X Eyepiece

② Binocular viewing head

①
③

Objective

Main body

Power cord

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 MB-135/MB-136

2.2 Assembly procedure

2.2.1Installing binocular
viewing head（Fig.1-2）

Insert the binocular viewing head

into the microscope head and turn it to

a proper position, then tight it.

Fig.1

2.2.2Installing the eyepiece

（Fig3-4）
Fig.2
Insert the eyepiece into the eyepiece

tube until they are against each other,

as shown in Fig.4.

Fig.3

Fig.4
4 5
 MB-135/MB-136

2.2.3Installing the objective（Fig.5-6）

1. Adjusting the coarse focus knob until the

support device of the mechanical stage
reaches its low limit position.

2. Screw the lowest magnification objective into

the nosepiece from the left or the right side,
then revolve the nosepiece clockwise and
mount other objectives by the sequence of
Fig.5
low to high magnification

Installing objective this way will make the

change of magnification to be easier during
using.

Clean the objective regularly, for lens is

susceptible to dust.

When operating, use 10 × magnification

objective to search and focus specimen
firstly, then replace with higher
Fig.6 magnification objective if necessary.

When replacing the objective, slowly turn

the nosepiece until you hear “clicked”,
that means the objective is in place.

2.2.4Mounting the filters（Fig.7）

1. Open the condenser carriage①；

2. Place the required filter②into the
condenser ， then close the condenser
carriage。

★the filter of the standard outfit is green and

baby blue.

Fig.7
 MB-135/MB-136

2.2.4Connecting the Power Cord（Fig.8-

10）

★The cable and cords are vulnerable when

②
bent or twisted, never subject the power cord
①
to excessive force.

Fig.8
1. Set the main switch①o “O” (off) state
before connecting the power cord.。
2. Insert the plugs②into the power jack③f
the microscope safely.
3. Plug the power cord④into the power
supply receptacle⑤. Make sure the
③
connection is well.

★Do use the supplied power cord all the time.

Fig.9 If lost or damaged, select the same
standard cord, please.

★A wide range of voltage ,like 100V～240V,

④ is acceptable for this microscope.

⑤
2.2.5Replacing the fuse（Fig.9-10）
Fig.10
Do remember to set the main switch①to the
state “O” (OFF) and unplug the power cord
before replacing the fuse. Rotate the fuseout of
the holder ③ , replace with a new fuse, then
rotate it back to the holder again.

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 MB-135/MB-136

2.2.8Installing and Replacing the bulb

（Fig.11-12）

Please use the specified halogen Lamp 6V20W.

① 1. Loosing the screw①。

2. Hold the bulb②after you wrap it with gauze or
other protection materials, and then insert its
pindeeply intothe jack in the lamp holder.
Fig11
3. Duringmicroscope using or soon after it is
turned off, the bulb, the lamp house and nearby
parts will be very hot and will cause serious
burns. Please turn the main switch to“O” (off)
and disconnect the power plug, and make sure
the bulb, the lamp room and periphery are all
cool. Then, you can do your replacing.
②
Insert the bulb gently. Squeezing too hard will
damage the bulb.

Fig12 To prevent reduced bulb life or cracking, do not

touch the bulb with bare hands. If fingerprints
are accidentally left on the bulb, wipe the bulb
with a soft cloth.

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 3. Adjustment and Operation MB-135/MB-136

3.1 Adjustment set diagram

Portrait Adjustment Knob

Aperture Iris Diaphragm adjustment

Lateral Adjustment Knob

Right Coarse Focus Knob

Main Switch

Tension Adjustment Collar Right Fine Focus Knob

Fig.13

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 MB-135/MB-136

Interpupillar DistanceIndicator

Condenser Focusing Knob

Left Fine Focus Knob Left Coarse Focus Clocking Set Brightness Adjustment Knob
Knob Fig.14
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 MB-135/MB-136

3.2 Adjustment and Operation

3.2.1Brightness Adjustment（Fig.15-16）

1. Connect the power cord and set the main switch

① to “－”state (ON).
2. Turning the brightness adjustment knob ②.

3.2.2Placing the Specimen（Fig.17）

① 1. Place the specimen the center of the mechanical

stage and use the stage clips to clamp it.
Fig.15 2. Turn the portrait and lateral adjustment knob①
of the mechanical ruler, move the specimen to
the required position.

 Be careful when changing the objective. If

you finish the observation with the short working
distance objective, and want to change another
one, becareful of not letting the objective touch
the specimen.
②
Fig.16
3.2.3Focusing the Specimen（Fig.18）

1.Focus the specimen with 10X objective. To avoid

the objective touching the specimen during focusing,
you should raise the mechanical stage to let the
specimen close to the objective at first, then slowly
① separate them to bring the specimen to focus.

Fig.17 Turn the coarse focus knob①conversely to lower the

specimen and search images in the 10×ocular
simultaneously, and thenuse the fine knob② to
focus. After that, you can replace with other
magnification objectives safely, and focus without
the risk of damaging the specimen.
②
① ③ To make the observation more convenient, you

Fig.18 can use the locking set③to fix the stage in a

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vertical direction.
 MB-135/MB-136

3.2.4Condenser Adjustment（Fig.19）

Turn the condenser focus knob①to move the

condenser up and down.Raise the condenser
when using the high magnification objective,
②
and descend it when using the low magnification
①
one.
1. Focus the specimen with 10× objective.
2. Adjust the condenser focus knob①to get a
clear image of the field iris diaphragm.
Fig.19 3. Turn the condenser centering knobs②to
center the image of field iris diaphragm in
the field of view.
The condenser and the objectiveare
coaxial. It has been adjusted before leaving
factory, so the user needn’t to adjust them by
selfThe highest position of the condenser
has been adjusted too. It also needn’t any
user’s operation. (The top surface of the
condenser is 0.03mm-0.4mm lower than the
stage top surface.)

3.2.5Aperture Iris Diaphragm

Adjustment（Fig.20）

Turn the aperture iris diaphragm stick①to adjust

the aperture iris diaphragm.

The aperture iris diaphragm is

designed for the adjustment of the numerical
aperture,not for the brightness.

Generally, setting the aperture iris

diaphragm to 70- 80% of the N.A. of the
objective in use will provide an image with
good contrast.If you want to observe the
①
image of the aperture iris diaphragm, remove
one eyepiece and look through the tube. You
will see a dark circle encroaching on the
bottom of the tube.

Fig.20 12
 MB-135/MB-136

3.2.6Adjusting the Interpupillary Distance

（Fig.21）

The interpupillary distance range:55mm～75mm。

While looking through the eyepieces, move both

eyepieces round until the left and right fields of view
coincide completely.

Fig.21
3.2.7Adjusting the Diopter（Fig.22）

1. Turn the eyepiece①to adjust the diopter while

looking through it.

★The diopter range of the eyepiece is±5

diopter.The number aligned to the line on the
viewing head is the diopter in use.

①
3.2.8Adjusting the tension adjustment
collar（Fig.23）

 Turn the tension adjustment collar①with your

fingers.When the collar is turned in the direction of
Fig.22 the arrow, thetension of the coarse adjustment
knob② increases. Turning the collar in the opposite
direction decreases the tension.

If the nosepiece descends on its own or if the

specimen gets out of focus quickly even when it is
brought into focus using the fine adjustment knob③,
it means the tension of the coarse adjustment knob is
too low. Turn the collar in the direction of the arrow
to increase the tension.
① ③
②

Fig.23
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 MB-135/MB-136

3.2.9Adjusting the Interpupillary Distance

（Fig.24）

The microscope with ECO：

ECO
The equipment can induct the front surroundings in
Fig.24 1m. At the moment, it light red and light source

keeping working.

When people left and no object within the front 1m,

the red light would flashed. After 15 minutes, light

source stop working.

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4．Technical Specifications MB-135/MB-136
4.1 Main Specifications
Optical System Limited optical system/Infinite optical system

Viewing Head Seidentopf Binocular Head, 30°Inclined,Interpupillary Distance 48-75 mm

Eyepiece WF 10X/18/WF 10X/20

Nosepiece Backward Quadruple Nosepiece

Objective Achromatic Objective/Infinite Semi-plan Objective4×，10×，40×、100×

Focusing Coaxial Coarse and Fine Adjustment, Moving Range 0.002mm，Fine Division 20mm

Condenser Abbe Condenser,NA1.25

Double Layers Mechanical Stage160×140，Moving Range74×50

Stage
TimingBelt Stage150×139mm，Moving Range 75×52mm

Illumination 1W S-LED /6V20W Halogen Lamp

4.2 Eyepiece and objectives

4.2.1 Achromatic Objective

NumericalAp
Focal distance Working Length
Magnification erture Focal Length(mm) Objective
（mm） （mm）
（NA）

4× 0.10 0.17 31.05 37.5 dry

10× 0.25 0.17 17.13 7.316 dry

40× 0.65 0.17 4.65 0.632 dry

100× 1.25 0.17 2.906 0.198 oil

4.2.2 Infinite Semi-plan Objective

Numerical
Focal distance Working Length
Magnification Aperture Focal Length(mm) Objective
（mm） （mm）
（NA）

4× 0.10 0.17 45 16.8 dry

10× 0.25 0.17 18 5.8 dry

40× 0.65 0.17 4.5 1.43 dry

100× 1.25 0.17 1.8 0.13 oil

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 MB-135/MB-136
4.2.3 Eyepiece

Focal Length
Eyepiece Magnification Field of View（mm）
（mm）

Wide field eyepiece 10× 24.95 Φ18

Wide field eyepiece 10× 25 Φ20

4.2.4 Total Magnification

Eyepiece 10× 10× 10× 10×

Objective 4× 10× 40× 100×

Total Magnification 40× 100× 400× 1000×

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5．Outfit MB-135/MB-136

Note：
“●”Standard Outfit, ○”optional

Standard Outfit
Item Specification Quantity
MB-135

Main Standard 1 ●

Main Standard（battery charging） 1 ○

Main Body Main Standard（ECO） 1 ○

Double Layers Mechanical Stage 1 ●

Condenser Holder 1 ●

Seidentopf Binocular Head 1 ●

Viewing Head
Seidentopf Trinocular Head 1 ○

Condenser Abbe Condenser, NA=1.25 1 ●

Nosepiece Backward Quadruple Nosepiece 1 ●

1W SLED 1 ●

6V20WHalogen Lamp 1 ○
Illumination
Blue, GreenFilter 1 each 〇

Spare Fuse 50T250V 500mA 2 ●

Eyepiece 10×Wide Field Plan Eyepiece 2 ●

Achromatic Objective4× 1 ●

Achromatic Objective10× 1 ●
Objective
Achromatic Objective40× 1 ●

Achromatic Objective100×（Oil,
1 ●
Spring）

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 MB-135/MB-136
Note：
“●”Standard Outfit, ○”optional

Standard Outfit
Item Specification Quantity
MB-136

Main Standard 1 ●

Main Standard（battery charging） 1 ○

Main Body Main Standard（ECO） 1 ○

Synchrone Belt Stage 1 ●

Condenser Holder 1 ●

Infinite Seidentopf Binocular Head 1 ●

Viewing Head
Infinite Seidentopf Trinocular Head 1 ○

Condenser Abbe Condenser, NA=1.25 1 ●

Nosepiece Backward Quadruple Nosepiece 1 ●

1W SLED 1 ●

6V20WHalogen Lamp 1 ○
Illumination
Blue, GreenFilter 1 each 〇

Spare Fuse 50T250V 500mA 2 ●

Eyepiece 10×Wide Field Plan Eyepiece 2 ●

Infinite Semi-plan Objective4× 1 ●

Infinite Semi-plan Objective10× 1 ●

Objective
Infinite Semi-plan Objective40× 1 ●

Infinite Semi-plan Objective100×（Oil,

1 ●
Spring）

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 6．Troubleshooting Guide MB-135/MB-136
6.1 Optical System
TROUBLE CAUSE SOLUTION

The nosepiece is not in the located position (objective and light path Locate the nosepiece properly where it
not coaxial) clicks
1. The edge of the
field of view is dark The surface of the lamp becomes black Change a new lamp bulb
or the brightness is
not uniform A lens (the objective, condenser, eyepiece or collector) is dirty. Clean it thoroughly

2. Dirt or dust is Dirt/dust on the specimen Replace with a clean specimen

visible in the field of
view Dirt/dust on the eyepieces Clean them

Specimen is not covered Add cover glass on it

Use standard cover glass with thickness

The thickness of the cover glass is not suitable
of 0.17mm

Specimen is placed reversely Turn it over

3. Visibility is poor
Dry objective has oil on it. (especially for 40X objectives) Wipe the oil
Image is not sharp;

A lens (the objective, condenser, eyepiece or collector) is dirty. Clean it

Contrast is poor;

Details are Immersion oil is not used with the 100x objective Use specified oil
indistinct
Air bubbles existed in the immersion oil Eliminate the bubble

Adjust the aperture iris diaphragm

The aperture iris diaphragm is stopped down too far
properly

Dirt or dust on the eyepiece Clean it

Center the condenser with the centering

Condenser is not properly centered
screw
4. One side of image
is blurred The nosepiece is not properly engaged Engage the nosepiece properly

The specimen is not clamped Clamp it with stage clips

The aperture iris diaphragm is too small Adjust it properly

5. The brightness is
not enough The condenser is too low Adjust it properly

A lens (the objective, condenser, eyepiece or collector) is dirty. Clean it

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 MB-135/MB-136
6.2 Mechanical System

TROUBLE CAUSE SOLUTION

1.Field of view of one

eye does not match that Interpupillary distance is incorrect Adjust interpupillary distance
of the other

The diopter is not proper Adjust the diopter properly

2. Observation is tiring
The brightness of the illumination is not
Adjust the lamp voltage
proper for eyes

6.3 Electrical System

TROUBLE CAUSE SOLUTION

No power supply Check the power cord connection

The pin of the bulb doesn’t insert

1.The bulb cannot light Insert the pin deeply
properly

The bulb broken Replace with a new one

2. The bulb burns out The bulb is not the specified one; Use the specified bulb;
suddenly The voltage is too high lower the voltage

3. the illumination is not

the voltage is too low Raise the voltage
bright enough

The bulb is about to burn out Replace with a new one

4. Image flicks
The bulb is not inserted deeply Check its connection

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