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Iat Enzy QP

This document outlines the internal assessment test for the B.E/B.Tech. degree in Biotechnology at Salem College of Engineering and Technology, focusing on Industrial Enzymology. It includes a structured examination format with three parts: Part A consists of short answer questions, Part B contains detailed questions with options, and Part C requires comprehensive answers. The test assesses various concepts related to enzymes, their mechanisms, kinetics, and factors affecting their activity.

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2 views2 pages

Iat Enzy QP

This document outlines the internal assessment test for the B.E/B.Tech. degree in Biotechnology at Salem College of Engineering and Technology, focusing on Industrial Enzymology. It includes a structured examination format with three parts: Part A consists of short answer questions, Part B contains detailed questions with options, and Part C requires comprehensive answers. The test assesses various concepts related to enzymes, their mechanisms, kinetics, and factors affecting their activity.

Uploaded by

reshmibiotech
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Reg. No.

SALEM COLLEGE
OF ENGINEERING AND TECHNOLOGY
B.E/B.Tech. DEGREE EXAMINATION, APR/MAY 2023
INTERNAL ASSESSMENT TEST – I
DEPARTMENT OF BIOTECHNOLOGY
BT3452 INDUSTRIAL ENZYMOLOGY Year/ Sem: II / IV
Date: Time: 3 hours Maximum: 100 marks
PART A-(10X2 =20 marks)
Answer All Questions
Q.
Question CO PO BTL
No.
1. Define Enzyme 1 1 1
2. Differentiate between cofactor and coenzyme 1 1 2
3. Describe the active site of the enzyme 1 1 1
4. Differentiate between absolute and stereochemical specificity 1 1 2
5. Describe the factor affecting enzyme activity 1 1 2
6. Write the equation for ES complex with its kinetics 2 1 2
7. Draw the LB plot for competitive inhibition 2 1 2
8. Define Km 2 1 1
9. Describe turnover number 1 1 1
10. Name the three plots used to determine Single substrate kinetics 2 1 2
Answer ALL Questions
PART B-(5X13=65 Marks)
11. a. Define enzyme and explain its history, importance and functions with
1 1 1
example (13)

(OR)
b. Write a note on Mechanism of enzyme action and its enzyme substrate
1 1 2
complex formation (13)
12. a. What are all the factors that affects the enzyme activity (13)
1 1 1
(OR)
b. Describe about enzyme catalysis and its mechanism (13) 1 1 2

13. a. Describe enzyme specificity and its types in detail (13)


1 1 2
(OR)
b. Derive Michaelis Mentons Equation of enzyme kinetics with graph (13)
2 2 3

14. a. Derive Lineweaver-Burk plot of enzyme kinetics with graph (13)


2 1 2

(OR)
b. Explain Eadie Hofstee plot and Hanes plot with graph (13)
1 2 3
15. a. Detail about types of enzyme inhibition with graph (13)
2 2 2

(OR)
b. Explain the effect of pH and temperature on enzymes and explain its
2 1 1
deactivation kinetics (13)
Answer ALL Questions
PART C-(1X15=15 Marks)
16. a. Detail about the nomenclature and classification of enzymes with example
1 2 2
(15)

(OR)
b. Explain multisubstrate enzyme kinetics with derivation (15)
2 2 3

BTL: 1-Remembering, 2- Understanding, 3- Applying, 4- Analyzing, 5- Evaluating, 6-Creating

SUBJECT INCHARGE HOD PRINCIPAL

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