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Technical Data
Tryptone Glucose Extract Agar M014
(Tryptone Glucose Yeast Extract Agar)
Intended use
Recommended for enumeration of bacteria in water, air, milk and dairy products.
Composition**
Ingredients g/L
Tryptone 5.000
Yeast extract 3.000
Dextrose (Glucose) 1.000
Agar 15.000
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 24.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates or
dispense as desired.
Principle And Interpretation
Tryptone Glucose Yeast Extract Agar was originally developed by Bowers and Hucker (1) which they called as Tryptone
Glucose Skim Milk Agar. Later on it was modified to the present composition for the cultivation and enumeration of
bacteria in air, water (2), milk and dairy products (3). Various authors have studied different aspects of this medium like
study of thermophilic bacteria in milk (4), influence of incubation temperature (5) etc. It is used as a standard medium for
the bacteriological plate count of milk and dairy products (6).
Tryptone, yeast extract provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamin B complex and
other essential growth nutrients. Glucose is the energy source. For the enumeration purposes, pour plate method is
suggested. Medium must be quickly poured into Petri dishes if milk sample is to be tested, because the milk may get
flocculated if the medium remains hot for longer period of time.
Type of specimen
Dairy samples; Water samples
Specimen Collection and Handling:
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards
(2). For dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3). After use,
contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established
guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
2. Further biochemical identification is necessary for confirmation.
3. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the
medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 2.4% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18- 48 hours.
Organism Inoculum Growth Recovery
(CFU)
** Bacillus spizizenii 50-100 good-luxuriant >=70%
ATCC 6633 (00003*)
# Klebsiella aerogenes 50-100 good-luxuriant >=70%
ATCC 13048 (00175*)
Escherichia coli ATCC 50-100 good-luxuriant >=70%
25922 (00013*)
Enterococcus faecalis ATCC 50-100 good-luxuriant >=70%
29212 (00087*)
Lactobacillus casei ATCC 50-100 good-luxuriant >=70%
9595
Pseudomonas aeruginosa 50-100 good-luxuriant >=70%
ATCC 27853 (00025*)
Staphylococcus aureus 50-100 good-luxuriant >=70%
subsp.aureus ATCC
25923 (00034*)
Key : (*) Corresponding WDCM numbers.
(#) Formerly known as Enterobacter aerogenes **Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact
with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
Reference
1.Bowers and Hucker, 1935, Tech. Bull., 228, N.Y.State Agr. Expt. Station.
2.Lipps WC, Braun-Howland EB, Baxter TE,eds. Standard methods for the Examination of Water and Wastewater, 24th
ed. Washington DC:APHA Press; 2023.
3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
4.Pickett, 1928, Tech. Bull. 147, N.Y. State Agr. Expt. Station.
5.Dennis and Weiser, 1937, J.Dairy Science, 20 : 445.
6.Abele C. A., Am. J. Pub. Health, 1939, 29: 821.
7.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
8.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual
of Clinical Microbiology, 11th Edition. Vol. 1.
Revision : 03/2024
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and
other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best
of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related
to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further
manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or
implied, and no liability is accepted for infringement of any patents.
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