Scribd
Upload
16 views34 pages

Genomics and Proteomics in Drug Discovery and Development

The document discusses the lengthy and costly process of drug discovery, highlighting the role of genomics and proteomics in identifying new drug targets and optimizing lead compounds. It explains the differences between genomics, which studies genes and their functions, and proteomics, which focuses on protein analysis and its dynamic nature. The integration of these technologies is expected to enhance drug discovery strategies and improve understanding of human diseases.

Uploaded by

Namira Shaikh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
16 views34 pages

Genomics and Proteomics in Drug Discovery and Development

The document discusses the lengthy and costly process of drug discovery, highlighting the role of genomics and proteomics in identifying new drug targets and optimizing lead compounds. It explains the differences between genomics, which studies genes and their functions, and proteomics, which focuses on protein analysis and its dynamic nature. The integration of these technologies is expected to enhance drug discovery strategies and improve understanding of human diseases.

Uploaded by

Namira Shaikh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 34

Genomics and Proteomics

in Drug Discovery and


Development
Drug Discovery

 It is a lengthy and a highly expensive process

 For the pharmaceutical industry, the number of years to bring a drug from
discovery to market is approximately 15 years, costing up to US$500
million per individual drug.

 A total of 40% of the compounds fail due to poor pharmacokinetics and


11% due to preclinical toxicity.

 It requires variety of tool from diverse fields.

 Several biotechnologies, including genomics, proteomics, cellular and


organismic methodologies have been developed.
Genomic and Proteomic technologies

 Genomic and Proteomic technologies have been developed over the last several years.

These methods are aimed at:


a) discovering new genes and proteins
b) quantifying and analyzing gene and protein expression
c) assigning functionality

 Being able to compare levels of gene and protein expression between diseased and normal
cells or cells treated with compounds, which vary in their efficacy and toxicity, could prove
valuable:
a) identifying new drug targets
b) optimizing the choice of lead compound candidates by more closely predicting their
success or failure
Genomics

 Study of genes and their function


 Aims to understand the structure of the genome (mapping of genes
and sequencing the DNA)
 Seeks to exploit the finding from the sequencing of the human and
other genomes to find new drug targets
Proteomics

 Proteomics is essentially protein analysis


 Could be described as a broad classification for a set of technology and
bioinformatics platforms aimed at the comprehensive molecular description of the
actual protein complement of a given sample.
 Presently, it is typically associated with systems biology.
 Progress in characterizing rapid posttranslational protein modifications
Difference between Genomics and
Proteomics

 Genome- relatively static


 Proteome- not static (it changes constantly in response to tens of thousands of intra-
and extracellular environmental signals).

 The proteome varies with various factors like health or disease, the nature of each
tissue, the stage of cell development, and effects of drug treatments.

 Proteomics runs parallel to genomics in many ways:


 Genomics starts with the gene and makes inferences about its products (proteins)
 Proteomics begins with the functionally modified protein and works back to the
gene responsible for its production
Process of drug discovery and
development
Steps of Drug Discovery Process

Target identification Biochemical assays and Lead optimizing to


and selection further testing improve potency

Cytotoxicity testing,
Target isolation and Docking of small
Toxicological
purification molecules
investigations

Analyse structures for


Structure
potential ligand Drug candidate
determination
binding sites
Identity comparison for new nucleic
acid sequences

Basic Local Alignment Search Tool (BLAST)


compare

New gene sequence known drug target


experimentally obtained genes

For example, tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a


known member named apoptosis ligand 2 (Apo2L) in the TNF family, was discovered
by searching homologs of the TNF family proteins in the expressed sequence tag
database.
Databases of model organisms

 There are over 30 model organisms for which whole genomes were sequenced, and
more than hundreds of organisms are being sequenced.
 The obtained data resources are helpful for functional predictions of genes,
especially for pathogenic organisms, and are useful for screening drug targets.
 For instance, this kind of whole-genome sequence information, integrated from
multiple pathogenic organisms, can be used to screen:
 race -specific genes
 virulence genes
 common genes of pathogenic organisms (conserved genes) specific bacterial or viral enzyme
genes
 bacterial membrane -translocation proteins
tissue-
specific
expression

analyze regulatory
gene databases network of
function a gene

select
candidate
drug target
genes
Analysis of gene expression profiles

Gene microarray – gene expression levels between two group of samples.


Analyze different cellular mRNA or reverse transcription products that are derived from different
individuals (healthy persons and patients), tissues, cell cycles, development periods,
differentiation periods, pathological change conditions, or stimulation conditions (including
different induction and treatments).
Analysis of the genes related to drug
action

 DNA microarray containing 97% of the predicted open reading frames to monitor
changes in Mycobacterium tuberculosis gene expression in response to the anti-
tuberculosis drug isoniazid.
 The results showed that isoniazid induced several genes that encode proteins that are
physiologically relevant to the drug’s mode of action, including an operonic cluster
of 5 genes encoding type II fatty acid synthase enzymes and fbpC, which encodes
trehalose dimycolyl transferase.
 Other genes, not apparently within directly affected biosynthetic pathways, also
were induced. These genes, efpA, fadE23, fadE24, and ahpC, likely mediate
processes that are linked to the toxic consequences of the drug.
 Insights gained from this approach may define new drug targets.
Gene reporter assays

Branched DNA amplification assay

Scintillation proximity assay

Rapid analysis of gene expression (RAGE)

Microarrays

Serial analysis of gene expression (SAGE)

Northern Blotting

PCR
CONCLUSION

 Knowledge of all the human genes and their functions may allow effective
preventive measures.
 The cause of common fatal diseases has been identified by genomics and it shows
the potential to identify individuals who are particularly susceptible to a given
disease long before that disease becomes apparent.
 It has positively impacted the drug research strategy and drug discovery
development processes.
 The process has been made simpler and economical. Further innovations in this
area are expected, which should take drug discovery research to a new level.
Genomic Approach to Drug
Discovery
Application of proteomics in drug
target discovery

 Proteomics is the large-scale study of the proteins in a cell, tissue, or entire


organism.
 Compare changes in protein levels in normal and diseased tissue.
 One established technique for comparative proteomics is based on labelling
proteins from normal and diseased tissues with different fluorescent dyes (Cy3
and Cy5), mixing the proteins together, and then separating them by isoelectric
point and molecular weight (difference in-gel electrophoresis).
 The sequencing of the human genome has increased interest in proteomics
because while DNA sequence information provides a static snapshot of the
various ways in which the cell might use its proteins, the life of the cell is a
dynamic process.
 This new data increase the interest of proteomics in the field of science, medicine,
and most notably – pharmaceuticals
 Proteomics can analyze biomarkers by quantifying individual proteins and show the
separation between one or more protein “spots” on a scanned image from two-
dimensional gel electrophoresis; for example, proteomic differences between early
and advanced stages of an illness can be observed.
Quantitative Proteomics

 Technique to determine the amount of proteins in the


sample.
 Mainly performed by 2D- gel electrophoresis and Mass
spectrometry.
Quantification using
spectrophotometry

 Concentration of a certain protein in a sample may be determined


 Concentration of a protein can be determined by measuring the OD at 280 nm on a
spectrophotometer, which can be used with a standard curve assay to quantify the
presence of Tryptophan, Tyrosine, and Phenylalanine.
DISADVANTAGES :
 Not the most accurate technique: composition of proteins can vary greatly and this
method would not be able to quantify proteins that do not contain the mentioned
amino acids.
 This method is also inaccurate due to the possibility of nucleic acid contamination.
 Other more accurate spectrophotometric procedures for protein quantification
include the Biuret, Lowry, BCA, and Bradford methods.
Quantification using two
dimensional electrophoresis

 2-DE provides information about the protein quantity, charge, and


mass of the intact protein.
 It has limitations for the analysis of proteins larger than 150 kDa or
smaller than 5kDa and low solubility proteins.
Quantification using mass
spectrometry

 Mass spectrometry (MS) represents one of the main technologies for


quantitative proteomics with advantages and disadvantages.
 Quantitative MS has higher sensitivity but can provide only limited information
about the intact protein.
 Quantitative MS has been used for both discovery and targeted proteomic
analysis to understand global proteomic dynamics in cells, tissues or organisms.
Quantitative Proteomics

stable isotope labeling with amino acids in isotope-coded affinity tags


cell culture
isobaric tags for relative and absolute
quantification
CONCLUSION

 Using the combination of genomics and proteomics, scientists can now


see every dimension of their biological focus, from genes, m RNA,
proteins and their subcellular localization.
 This will greatly assist our understanding of the fundamental
mechanistic basis of human disease and allow new improved and
speedier drug discovery strategies to be implemented.

You might also like