RAMA Medical College Hospital and Research Centre, Hapur, Uttar Pradesh

M.B.B.S. First Professional (Batch 2024-25)

Biochemistry
Part Completion Test
Timie: 02:00 PM – 04:00 PM Maximum Marks: 100
∙ The question paper contains two sections A and B
∙ Section A to be answered in first 20 minutes. Changing of answer (option) of MCQs is not
allowed and zero mark will be given for changing option.
∙ Attempt all questions and answer should be specific to the questions
∙ Draw neat diagram wherever it is necessary
 Section A
Multiple Choice Questions (20)
1. Enzyme used in treatment of myocardial infarction-
a) Streptokinase
b) Asparaginase
c) Creatine kinase 2
d) Creatinine transferase
2. Phosphofructokinase -1 of glycolysis is
allosterically activated by-
a. ATP
b. Fructose 1,6- bisphosphate
c. Citrate
d. Fructose2,6 bisphosphate
3. Enzyme involved in both glycolysis and
gluconeogenesis is-
a. Pyruvate kinase
b. Aldolase
c. Glucokinase
d. Glucose 6 phosphatase
4. Predominant clinical feature of pyruvate kinase
deficiency is-
a. CNS symptoms
b. Hemolytic anemia
c. Cataract
d. Bone marrow suppression
5. Tetany is a feature of-
a. Hypercalcemia
b. Hypocalcemia
c. Hyperkalemia
d. Hyperkalemia
6. Vitamin K dependent clotting factor is-
a. Factor I
b. Factor VII
c. Factor XI
d. Factor XII
7. Major storage and transport form of vitamin D3 is-
a. Cholecalciferol
b. 24,25, dihydroxy cholecalciferol
c. 1,25 dihydroxy cholecalciferol
d. 24 hydroxy cholecalciferol
8. Inhibition of glycolysis by oxygen is called-
a. Pasteur effect
b. Bohr effect
c. Hamburger effect
d. Anaerobic glycolysis
9. The following pathways occurs in cytosol except-
a. Glycogenolysis
b. Glycogenesis
c. Kreb cycle
d. Anaerobic glycolysis
10. The key regulatory step of fatty acid synthesis is
catalyzed by-
a. Malonyl transferase
b. Acetyl coA carboxylase
c. Enoyl reductase
d. Citrate lyase
11. Activation of acetoacetate to acetoacetyl CoA by
thiophorase reaction does not occur in-
a. Liver
b. Kidney
c. Brain
d. Adipocytes
12. During fatty acid synthesis acetyl CoA is shuttled into
the cytoplasm as-
a. Malate
b. Citrate
c. Succinate
d. Malonyl coA
13. Only fat-soluble vitamin that can serve as a coenzyme-
a. Vitamin A
b. Vitamin E
c. Vitamin D
d. Vitamin K
14. Which of the following function as both hormone
and visual pigment?
a. Thiamine
b. Retinal
c. Riboflavin
d. Folic acid
15. Which of the following process occurs in the
formation of disulfide bridge between two cystine
residues?
a. Reduction of sulfhydryl group
b. Electrostatic interaction
c. Oxidation of sulfhydryl group
d. Hydrogen bond formation
16. The enzyme amylase can break glycosidic linkages
between glucose monomers only if the monomers
are the alpha form, which of the following could
amylase breakdown?
a. Cellulose
b. Glycogen
c. Chitin
d. A & C only
17. Enzyme inhibition is applicable in all except:
a. Regulation of metabolic pathways
b. Use of drugs as enzyme inhibitors
c. Enhance the action of toxins
d. To check access effect of enzyme
18. All are mechanisms of enzyme regulation in
biological systems except:
a. Covalent modifications
b. End product inhibition
c. Enzyme induction
d. Enzyme denaturation
19. The structure of vitamin A contains-
a. Chromane ring
b. Beta ionone ring
c. Thiazole ring
d. Naphthoquinone ring
20. Acetyl CoA carboxylase is activated by-
a. Palmityl-CoA
b. CO2
c. Citrate
d. Malonyl CoA
 Section B
Clinical case- (15marks)
23-year-old man was given Antimalarial drug (Primaquine) for his fever. Soon after, he developed pain in his
abdomen and vomiting. His urine was dark yellow and sclera is also showed yellowish discoloration.
Laboratory investigations: -
Investigations Reference Range
Haemoglobin – 9.8 gm/dL 14-16 gm/dL
Serum bilirubin- 13.4 mg/dL 0.3-1.2 mg/dL
SGOT - 78 U/L up to 35 U/L
SGPT – 66 U/L up to 40 U/L
Peripheral smear examination showed evidence of hemolysis
a. Identify the deficient enzyme.
b. Why the above enzyme deficiency results in hemolysis?
c. Explains biochemical basis of all lab-investigations?
d. What is the significance of this pathway involved?
e. Does this disorder offer any advantage also?
 Explain why? (25 marks)
i. After extraneous exercise muscle cramps develop.
ii. HDL is called as good cholesterol.
iii. Cyanide is highly toxic for body.
iv. Excessive ketone bodies are generated in uncontrolled Diabetes mellitus.
v. TCA is said to be an amphibolic pathway.
Short notes- (25 marks)
i. Diagnostic importance of isoenzymes giving few examples.
ii. Visual cycle
iii. Familial hypercholesterolemia
iv. Reverse cholesterol transport
v. Special products generated from cholesterol.
Compare and contrast- (15 marks)
i. Glucogenic amino acid vs ketogenic amino acid
ii. Bile acid vs bile salts
iii. Synthase vs synthetase enzymes.