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Upstream and Downstream Processing

Fermentation technology consists of two main stages: upstream processing, which involves strain selection, media preparation, sterilization, and inoculum development, and downstream processing, which includes cell separation, product extraction, purification, and drying and packaging. Upstream processing focuses on preparing for fermentation, while downstream processing deals with recovering and purifying the final product. Both stages are essential for efficient fermentation and product quality.

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13 views

Upstream and Downstream Processing

Fermentation technology consists of two main stages: upstream processing, which involves strain selection, media preparation, sterilization, and inoculum development, and downstream processing, which includes cell separation, product extraction, purification, and drying and packaging. Upstream processing focuses on preparing for fermentation, while downstream processing deals with recovering and purifying the final product. Both stages are essential for efficient fermentation and product quality.

Uploaded by

markhourai
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Upstream and Downstream Processing

Fermentation technology is divided into two main stages:

Upstream Processing

This includes all the steps leading to fermentation:

●​ Strain Selection and Improvement: Selecting or engineering high-yielding microbial strains.​

●​ Media Preparation: Providing essential nutrients like carbon, nitrogen, vitamins, and minerals.​

●​ Sterilization: Ensuring a contamination-free environment.​

●​ Inoculum Development: Scaling up microbial culture before inoculating the main fermenter.​

Downstream Processing

These are post-fermentation steps to recover and purify the product:

●​ Cell Separation: Removing microbial biomass using centrifugation or filtration.​

●​ Product Extraction: Solvent extraction, precipitation, or chromatography.​

●​ Purification: Refining the product to acceptable purity levels, especially for pharmaceuticals.​

●​ Drying and Packaging: Final processing for storage and distribution.

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