XCELIS® Saccharification & Fermentation

DISTI L L ASE® CS
Saccharifying enzyme for ethanol
production

Description Application recommendations

DISTILLASE® CS is an optimized, concentrated blend of DISTILLASE® CS enzyme is used to saccharify liquefied starch
enzymes that produces glucose from liquefied starch. from various sources including wheat, corn, sorghum, barley, rye,
DISTILLASE® CS contains glucoamylase (or amyloglucosidase) rice and cassava. The resultant glucose product is fermented by
produced with Trichoderma reesei and alpha-amylase also yeast to yield ethanol.
produced with Trichoderma reesei. The glucoamylase in
DISTILLASE® CS enzyme catalyzes the release of successive
Recommended operational conditions
glucose units from the nonreducing ends of soluble dextrins and
oligosaccharides, while the alpha-amylase at the same time can
Optimum pH range 4.0–5.0
generate those oligosaccharides and dextrins.
Effective pH range 3.2–5.8
Typical characteristics Effective temperature 28–65°C (83–149°F)
Activity 570 GAU/g (minimum)
Appearance Clear brown liquid 1–16 hours
Pre-saccharification
pH 4.0–4.5 at 60–65°C (140–149°F)
Specific gravity 1.13–1.16 Please contact your IFF Technical
Formulation Food-grade DISTILLASE® CS dosage Representative for recommendations
specific to your liquefaction process
One Glucoamylase Unit (GAU) is the amount of enzyme that
will liberate one gram of reducing sugars, calculated as glucose
per hour, from a soluble starch substrate under the specified Dosage recommendations
conditions of the assay. A detailed assay method is available upon DISTILLASE® CS enzyme is generally added at a level of 0.055 to
request. 0.075% w/w (starch, dry solid basis). This corresponds to a level
of 0.375 to 0.50 kg DISTILLASE® CS / MT grain ‘as is’. The actual
Performance benefits enzyme requirement is dependent on the temperature, reaction
DISTILLASE® CS enzyme provides the following benefits to time and pH of the individual plant saccharification/fermentation
ethanol producers: process, and its desired results. Fermentation performance
• Increased ethanol yield by complete saccharification of may be enhanced by the addition of a fungal protease like
liquefied starch FERMGEN®.
• Less unfermented starch in stillage and DDGS
• Improved performance in pre-saccharification processes Complete saccharification of starch
• Increased fermentation rates and shorter fermentation times DISTILLASE® CS enzyme converts more starch to fermentable
sugars compared to other comparable products.
Figure 1
Effect of initial fermentation pH on conversion efficiency with DISTILLASE® CS enzyme Storage
(whole ground wheat, 30% DS, 32°C) To ensure maximum retention of activity, store DISTILLASE® CS
enzyme under refrigerated conditions with the container closed.
Relative conversion efficiency

100%
Prolonged storage at elevated temperature should be avoided.
80%

60%
Modern biotechnology
The enzymes are manufactured by fermentation of
40% microorganisms that are not present in the final product. The
microorganisms have been optimized by means of modern
20%
biotechnology. This product does not contain genetically
0% engineered material from the microorganisms.
3.0 3.5 4.0 4.5 5.0 5.5 6.0

Initial pH
Safety & enzyme handling
Avoid generation of dust and aerosols. Avoid breathing dust or
Figure 2 spray mist. In case of contact with skin or eyes, promptly rinse
Effect of DISTILLASE® CS enzyme and conventional glucoamylase on residual starch
of fermented wheat mash with water for at least 15 minutes. Use of appropriate personal
protective equipment is recommended. For additional detail
0,8
Unfermented starch in stillage

0,7
conventional GA regarding safe enzyme handling, please refer to the appropriate
0,6
Safety Data Sheet (SDS), the Working Safely with Enzyme
0,5
DISTILLASE® CS handbook published by the Enzyme Technical Association
(% DS)

0,4 (ETA) and the Guide to the Safe Handling of Microbial Enzyme
0,3 Preparations published by the Association of Manufacturer and
0,2 Formulators of Enzymes (AMFEP). These references are available
0,1 from IFF.
0

DISTILLASE® CS
(0.40 kg/MT)
conventional enzyme
(0.48 kg/MT)
Technical service
IFF is committed to working with ethanol producers to achieve
Glucoamylase used in SSF
their goals. More detailed information about the application of
this product is available upon request. If you have any questions,
Regulatory status please call us and let us know how we can be of assistance.
Please contact your IFF representative to learn more about this
product’s regulatory status.

Packaging
DISTILLASE® CS enzyme is available in various package sizes.
Please consult your IFF representative for detailed information.

www.xcelis.com

©2021 International Flavors & Fragrances Inc. (IFF). IFF, the IFF Logo, and all trademarks and service marks denoted with
™, SM or ® are owned by IFF or affiliates of IFF unless otherwise noted. The information provided herein is based on data
IFF believes, to the best of its knowledge, reliable and applies only to the specific material designated herein as sold by IFF.
The information contained herein does not apply to use of the material designated herein in any process or in combination
with any other material and is provided at the request of and without charge to our customers. Accordingly, IFF cannot
guarantee or warrant such information and assumes no liability for its use. Other than as may be expressly set forth in a
contract of sale, IFF makes no warranty, express or implied, as to the material set forth herein, including the warranty of
merchantability or fitness for a particular use.

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