30 Risk Assessment Guidance 2,4-dinitrophenylhydrazine
2,4-dinitrophenylhydrazine 2,4-DNPH (used to prepare Brady’s reagent) (NO2)2C6H3NHNH2 (198.14)
Flammable solid [H228]. Harmful if swallowed [H302]. Some suppliers may also indicate one or more
of: Causes skin irritation [H315]. Causes serious eye irritation [H319].
2,4-dinitrophenylhydrazine is dangerous in contact with:
• OXIDISING AGENTS. METAL OXIDES. Vigorous or explosive reactions may occur.
• IGNITION SOURCE/SPARK. Risk of dust explosion if sufficient quantity of dry powder is in the air.
The storage and use of 2,4-DNPH is affected by the current Explosives Regulations.
DANGER See Fireworks and explosives. Contact CLEAPSS for further advice.
Storage code: T (Organic) [Orange-red solid]
• The solid is supplied ‘wetted’ to minimise the risk of explosion. To keep the solid damp, stand the
bottle inside a larger (labelled) container that also contains a little tap water (~ 1 cm depth).
Storage
Protect the bottle’s label.
• If incorrectly stored, the solid may have become dry and more sensitive to shock or friction.
Do NOT attempt to open the bottle; contact CLEAPSS.
Phenylhydrazine C6H8N2 (108.14)
Do NOT use this compound.
Toxic if swallowed, in contact with skin & if inhaled [H301, H311, H331]. Causes skin and serious eye irritation [H315,
H319]. May cause an allergic skin reaction [H317]. Suspected of causing genetic defects [H341]. May cause cancer
DANGER [H350]. Causes damage to organs through prolonged or repeated exposure [H372]. Very toxic to aquatic life [H400].
Storage code: T (Organic) [Colourless-yellow liquid/solid (melting point is ~ 20 oC)]
Storage
• Storage is prior to disposal, W1.
Follow standard procedures (see Emergency Hazcards and GL 120). NOTE:
Emergencies
• General spills, 2,4-DNPH: Keep moist. Scoop into a suitable container. Thoroughly rinse spill area.
Read this Hazcard in conjunction with About Hazcards (guide GL 120) Hazcards 2016 Edition ©CLEAPSS (Updated: 11/19)
30 Risk Assessment Guidance 2,4-dinitrophenylhydrazine
The storage and use of 2,4-DNPH is affected by the current Explosives Regulations. See Fireworks and explosives.
Contact CLEAPSS for further advice.
Detailed guidance on specific activities and techniques involving these substances can be found at: www.cleapss.org.uk
General use of: Hazard information User Suggested general control measures and guidance
• Wear eye protection.
2,4-dinitrophenyl • Take particular care to avoid skin contact (see GL 120). Wear gloves
hydrazine, TT when transferring the solid or preparing/dispensing Brady’s reagent.
2,4-DNPH DANGER Note: Skin is stained yellow which may be followed by dermatitis.
(See reverse) • Protect the bench and working area from stains.
Other notes; see also detailed activity-specific guidance:
• Preparation of Brady’s reagent (TT): See Recipe 33. Prepare only
when required and just sufficient for the lesson. Do not store any
unused reagent, dispose of it promptly.
Brady’s reagent See Other notes. Y12 • Identifying carbonyl compounds using Brady’s reagent: See activity-
specific guidance for detailed method and disposal of test residues.
Disposal: W1, 2,4-DNPH
W7, unused Brady’s reagent; see below.
Follow general guidance in About Hazcards (GL 120). NOTE for small amounts only:
Disposal • W7 (unused Brady’s reagent): Dilute the solution in a full bucket of water and pour the contents
down a foul-water drain. Flush away with more water.
Read this Hazcard in conjunction with About Hazcards (guide GL 120) Hazcards 2016 Edition ©CLEAPSS (Updated: 11/19)
