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04-06 Task

The document outlines a lab assignment on electrophoresis, detailing the differences in DNA among individuals, the PCR process, and its applications in forensic science. It includes questions on DNA amplification, gel electrophoresis, and potential errors in DNA fingerprinting. The assignment requires students to analyze DNA evidence and compare it with a victim's DNA.

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30 views2 pages

04-06 Task

The document outlines a lab assignment on electrophoresis, detailing the differences in DNA among individuals, the PCR process, and its applications in forensic science. It includes questions on DNA amplification, gel electrophoresis, and potential errors in DNA fingerprinting. The assignment requires students to analyze DNA evidence and compare it with a victim's DNA.

Uploaded by

makalagreene0
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Name: Makala Greene School: Miracle Academy

Date: December 2 , 2024 Facilitator: Ms.Bush

4.06 Electrophoresis Lab (35 points)


1. List four ways DNA can differ between people. (2 points)
a. single pairs
b. deletions
c. insertions
d. duplication

2. Describe the process of PCR. (3 points)


It is a process that allows the copying of small select strands of DNA.

3. Explain why, when PCR is used to amplify the same region of DNA from two different
people, the size of the DNA fragment(s) generated may be different. (3 points)
At some locations individuals DNA differs. The different s can be in single pairs of
DNA or in the insertion delectation or duplication of DNA sequences

4. After one round of PCR, there are two copies of the targeted DNA sequence. After two
rounds, there are four copies. How many copies of the targeted DNA sequence should be
present after six rounds of PCR? Show how you arrived at your answer. (3 points)
There will be 64 copies of the DNA sequence because each PCR cycle
doubles the number of DNA molecules. After 6 cycles, you will have 2 raised
to the power of 6, which equals 64 copies. 2x2x2x2x2x2=64
5. How is PCR useful in forensic science? (3 points)
PCR allows us to take small amounts of evidence and copy it several times for analysis.
It also allows us to create DNA fragments of different lengths to help distinguish between
individuals.

6. Describe the process of gel electrophoresis. (3 points)


Gel electrophoresis separates DNA fragments by size using an electric current. DNA,
being negatively charged, moves through a gel matrix towards a positive electrode.
Smaller fragments travel faster, resulting in bands visible after staining.

7. What characteristic of the DNA molecule allows us to separate DNA molecules by size?
(2 points) The negative charge on the DNA molecules allows us to use the electric
current to separate the DNA molecules.

© ACCESS Virtual Learning 2021


8. Use the evidence from the virtual lab to create a graphic of the DNA fingerprints. You
may draw and insert a picture or use the table below as a template. (6 points)
Suspect 2
Suspect 1
Victim
Evidence

9. Based on the evidence, is either suspect tied to Devin’s assault? Explain your answer. (3
points)

10. Compare the pattern of the evidence DNA with the victim's DNA. Is the evidence DNA
contaminated with the victim's DNA? (1 point)

11. If the evidence was contaminated with the victim’s DNA, how would the banding pattern
differ? (3 points)

12. Where is there possibility for error in the gel electrophoresis / DNA fingerprinting
process? (3 points)

© ACCESS Virtual Learning 2021

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