USER’S MANUAL

For DU-8200 Series

Spectrophotometers

Drawell Scientific
 Contents

Chapter 1 Preface……………………………………………………………………………1
1. Safety …………… ………………………………………………………………… 1
2. General……………………………………………………………………………………1
3. Electrical … ………………………………………………………………………………1
4. Warning…………………………………………………………………………………………………….. 1
5. Performance……………………………………………………………………………………………….. 2
6. Radio Interference ………………………………………………………………………………………2

Chapter 2 Introduction of the Instrument.........................................................3

1. Brief Introduction of the instrument...................................................................3
2. Working Principle………………………………………………………………………4
3. Structure...........................................................................................4
4. Unpacking Instructions………………………………………………………………………………….5
5. Specifications……………………………………………………………………………………………….5

Chapter 3 Install Instrument……………………………………………………………6

1. Environment Required…………………………………………………………………6
2. Voltage Confirmation…………………………………………………………………6
3. Installation………………………………………………………………………………6

Chapter 4 Operation Introduction………………………………………………………7

1. Software Structure………………………………………………………………………7
2. Basic Operation…………………………………………………………………………8
2.1 Choose Photometric Mode……………………………………………………… 8
2.2 Set Wavelength …………………………………………………………………… 8
2.3 Set Parameters…………………………………………………………………… 8
2.4 Set the position of auto-cell holder……………………………………………… 8
2.5 Delete the Input Value…………………………………………………………… 8
2.6 Delete the test result and stored data……………………………………………8
2.7 Calibrate 100%T/0Abs…………………………………………………………… 8
2.8 Measure Samples………………………………………………………………… 8
2.9 Print the test result…………………………………………………………………9
3. Preparation before test………………………………………………………………9
3.1 Switch on the instrument and begin the system self-test………………………9
3.2 Pre-warm……………………………………………………………………………9
3.3 Check the cuvettes…………………………………………………………………9
4. Measurement……………………………………………………………………………9
4.1 Photometry…………………………………………………………………………9
4.2 Quantitative………………………………………………………………………11
4.3 Setup ……………………………………………………………………………17
 1) Power On/Off D2 Lamp………………………………………………………18
2) Power On/Off W Lamp………………………………………………………18
3) Set Date and time……………………………………………………………19
4) Calibrate Dark Current………………………………………………………19
5) Wavelength Calibration………………………………………………………20
6) Input the lamps changing Wavelength Point………………………………20
7) Reload Default…………………………………………………………………21
8) Version…………………………………………………………………………21
Chapter 5 Instrument Maintenance……………………………………………………22
1. Daily Maintenance……………………………………………………………………………………….22
2. Trouble shooting………………………………………………………………………………………….22
3. Sparepart Replacement………………………………………………………………………………..23
 CHAPTER 1 PREFACE
1, Safety
The safety statements in this manual comply with the requirements of the HEALTH
AND SAFETY AT WORK ACT, 1974.

Read the following before installing and using the instrument and its accessories.
This instrument should be operated by appropriate laboratory technicians.

2, General
The apparatus described in this manual is designed to be used by properly trained
personnel in a suitable equipped laboratory. For the correct and safe use of this
apparatus it is essential that laboratory personnel follow generally accepted safe
procedures in addition to the safety precautions called for in this manual.

The covers on this instrument may be removed for servicing. However, the inside of
the power supply unit is a hazardous area and its cover should not be removed under
any circumstances. There are no serviceable components inside this power supply
unit. For this instrument, avoid touching the high voltage power supply at all times.

Some of the chemicals used in spectrophotometry are corrosive and/or inflammable

and samples may be radioactive, toxic, or potentially infective. Care should be taken
to follow the normal laboratory procedures for handling chemicals and samples.

3, Electrical
Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Installation).

The power cord shall be inserted in a socket provided with a protective earth contact.
The protective action must not be negated by the use of an extension cord without a
protective conductor.

4, Warning
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.

Whenever it is likely that the protection has been impaired, the apparatus shall be
made inoperative and be secured against any unintended operation.

Note ： NEVER touch or handle the power supply on this instrument due to the high
voltage！
The protection is likely to be impaired if, for example, the apparatus

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 ▲Shows visible damage
▲Fails to perform the intended measurements
▲Has been subjected to prolonged storage under unfavorable conditions
▲Has been subjected to severe transport stresses

5, Performance
To ensure that the instrument is working within its specification, especially when
making measurements of an important nature ， carry out performance checks with
particular reference to wavelength and absorbance accuracy. Performance checks are
detailed in this manual.

6, Radio Interference
For compliance with the EMC standards referred to in the EC Declaration of
Conformity, it is necessary that only shielded cables supplied by us are used when
connecting the instrument to computers and accessories.

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 CHAPTER 2 INTRODUCTION OF THE INSTRUMENT
1. Brief Introduction of the Instrument
This instrument is a single beam, general purpose instrument designed to meet the
needs of the Conventional Laboratory, This instrument is ideal for various applications,
such as: Chemistry, Biochemistry, Petro-chemistry, Environmental Protection, Food
and Beverage Labs, Water and Waste Water Labs and other fields of quality control
and research.

This instrument incorporates a 128 × 64 dots matrix LCD display for photometric
results, easy operation and wavelength range of 190nm to 1100nm. This instrument is
ideal for measurements in the visible and ultraviolet wavelength region of the
electromagnetic spectrum.

Fig 2-1 Front View

Fig 2-2 Control Panel

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2. Working Principle
Different matter has different but special absorbance wavelength point. Also, when at
the fixed wavelength point, the absorbance has some relation to the substance’s
(Always transparent Solution) concentration and its thickness. The relation can be
concluded as the following Formula which is called Lambert-Beer Law.
T= I/Io A=KCL= -log I/Io
A Absorbance
C Concentration of the Solution
K Absorbance Coefficient of the Solution
L The length of the Solution in the light path
I The intensity of the light focused on the A/D after it permeate the
solution to be measured.
Io The intensity of the light focused on the A/D after it permeate the
Solution.
Note: When test, the solvent is usually taken as the Reference Solution and its
Transmittance is considered as 100%T. While the Transmittance of the sample to
be tested is a relative value which is got comparing to that of the Reference.

3. Structure
The spectrophotometer consists of five parts:
1) Halogen or deuterium lamps to supply the light;
2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted
second order radiation;
3) A sample compartment to accommodate the sample solution;
4) Detector to receive the transmitted light and convert it to an electrical signal; and
5) A digital display to indicate absorbance or transmittance. The block diagram (Fig
2-4) below illustrates the relationship between these parts.

Fig 2-4 Block diagram for the Spectrophotometer

In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The
grating disperses the light beam to produce the spectrum, a portion of which is focused
on the exit slit of the monochromator by a collimating mirror. From here the beam is
passed to a sample compartment through one of the filters, which helps to eliminate
unwanted second order radiation from the diffraction grating. Upon leaving the
sample compartment, the beam is passed to the silicon photodiode detector and
causes the detector to produce an electrical signal that is displayed on the digital
display.

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4. Unpacking Instructions
Carefully unpack the contents and check the materials against the following packing
list to ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer...................................................................... …….… 1
Power Cord................................................................................... …….… 1
1cm Glass Cuvettes………………………………………..…………… 1 Set of 4
1cm Quartz Cuvettes………………………………………...…………. 1 Set of 2
Operation Manual........................................................................ …….…. 1

5. Specifications

UV-VIS VIS
Wavelength Range 190-1100nm 320-1100nm
Band Width 4nm(2nm optional) 4nm
Wavelength Accuracy ±1nm
Wavelength Repeatability 0.3nm
Wavelength Setting Auto
Photometric Accuracy ±0.5%T
Photometric Repeatability 0.3%T
Photometric Display Range 0-200%T, -0.3-3.0A
Stability 0.002A/h @ 500nm
Stray Light ≤0.2%T@220nm, 360nm
Data Output Port USB
Display 128*64 Dots LCD
Lamps Deuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiode
Power Requirement 220V/50Hz or 110V/60Hz
Dimension (L*W*H) 431*318*195mm
Net Weight 12kg

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 CHAPTER 3 INSTALL INSTRUMENT
1. Environment Required
To ensure the best performance, the following conditions are required:

 The best working temperature range is 16-30℃ and the humidity is 45-80%.
 Keep it as far as possible away from the strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields.
 Set the unit up in an area that is free of dust, corrosive gases and strong
vibrations.
 Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
 The power requirement is 220±22V@50±1Hz or 110±11V@60±1 Hz。
 Use the appropriate power cord and plug into a grounded outlet.
 If the local voltage is not stable enough, a manostat is required.
 Be away from direct sunlight

2. Voltage Confirmation

Be sure to set the instrument’s Voltage Switch at your local power

supply, or severe damage may occur! The Voltage Switch is on the
bottom of the instrument. （The default value is 220V, you can see
the value on the Voltage Switch）

3. Installation
Step 1: Check the packing list
Unpack the contents, check the materials with the Packing List. Any damage or
Lost found, please contact us or the local dealer.
Step 2: Position
Place the instrument on the stable table carefully.
Step 3: Install printer (Optional)
Make sure the printer is power off; link the printer’s data cable to the
instrument’s parallel port.
Step 4: Link the power cord
Make sure the instrument’s power switch is in the Off condition, link the power
cord to the instrument and insert another end in the socket provided with a
protective earth contact.
Step 5: Switch on the power
Check again, make sure that all the links are right. Switch on the power. The
instrument can be used to measure after self-test and pre-warm.

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 CHAPTER 4 OPERATION INTRODUCTION
1. Software Structure
1.1 Menu Tree

Fig 4-1 Menu Tree

1.2 Firmware Functions
The firmware consists of 3 functions: Basic Mode, Quantitative, System Functions.
1) Basic Mode
Measure the Absorbance, Transmittance or Energy in a fixed wavelength point.
The test result can be stored in the RAM.
2) Quantitative
Standard Curve Method：Set up standard curve by standard samples; use the
new curve to measure the unknown sample’s concentration. The curves and the
test results con be stored in the RAM.

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 Coefficient Method: Input the values of the curve equation’s coefficient, and
then measure the unknown sample’s concentration.
3) Setup
Light source management; Get Dark Current; Calibrate Wavelength and Set time,
etc.

2. Basic Operation
2.1 Choose Photometric Mode

Press Arrow key to choose and to confirm.

2.2 Set Wavelength

In the measurement Interface，press to set wavelength，use Arrow

keyboard to input the value，press to confirm and set 100%T/0Abs

automatically.

2.3 Set Parameters

Press to go into the parameter setting interface，press Arrow Key to

choose the corresponding menu and use Arrow key to input the parameter, press

to confirm，press to return.

2.4 Set the position of auto-cell holder. （The Auto Cell Holder is optional）

In the measurement Interface, press first, then press the Arrow key of
1-8, and then the corresponding cell will be positioned at the light path.

2.5 Delete the Input Value

Press to delete the last character of the input.

2.6 Delete the test result and stored data

In the measurement Interface，press to delete the test result and

stored data.

2.7 Calibrate 100%T/0Abs

Put the reference in the light path, press to calibrate 100%T/0Abs。

2.8 Measure the samples

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 Put the samples in the light path and press to measure.

2.9 Print the test result

In the Measurement Interface, press to print the result..

3. Preparation before test

3.1 Switch on the instrument and begin the system self-test(Diagnostic
Program)
Make sure to remove all the blocks in the light path; Close the compartment lid
and switch on the power; Then the system will begin self-test.
Note: During the Diagnostic Program runs, please DON’T open the lid of
the compartment!
3.2 Pre-warm
When the diagnostic program finished, it goes into pre-warm condition. 20
minutes is required before measure.

3.3 Check the Cuvettes

The cuvette should be clear and there’s no solution remains on the transparent
surface. If the wavelength is below 340nm, Silicon Cuvette is a must.

4. Measure

4.1 Basic Mode

Step 1, Go into Basic Mode interface

In the main menu, use Arrow Key to choose “Baisc Mode”，press

to go into corresponding setting interface.（Fig. 4-2）
；

Fig. 4-2

Step 2, Set Wavelength

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 Press to set Wavelength，input the wavelength value by the Arrow

Keypad, then press to confirm.（Fig. 4-3）

；

Fig. 4-3

Step 3, Set Photometric Mode

Press to go into Parameter Setting Interface，press Array Key to

select “Abs”、“%T” or “Energy” mode，press to confirm. Press

to return（Fig. 4-4）；

Fig. 4-4

Step 4, Go into Continuous Measurement Interface

Press to go into continuous measurement interface（Fig. 4-5）；

Fig. 4-5

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 Step 5, Calibrate 100%T/0Abs

Put the Reference Solution in the light path，press to calibrate

100%T/0Abs；

Step 6, Measure Sample

Put the unknown concentration sample in the light path, press to

measure，and the result displays in the data sheet. At the same time, the test
result will be stored in the RAM automatically.
Repeat this step to finish all the samples measurement.

Step 7, Print the data

Press to print the test result.

Step 8, Delete the data

Press to delete the test data.

4.2 Quantitative

Step 1: Go into Quantitative Interface

In the main menu, press Arrow Key to choose “Quantitative”; Press

to enter the Quantitative method selecting Interface.（Fig. 4-6）；

Fig. 4-6

Step 2: Choose Unit

Move the cursor on “Unit”, press to go into concentration unit

choosing interface. (Fig. 4-7) Use Arrow Key to move the cursor on the unit

you want, and press to confirm.

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 Fig. 4-7

Step 3: Choose Methods

There are two methods to be selected: Standard Curve and Coefficient.
I. Standard Curve：
(1) Go into “Standard Curve”Interface
In “Quantitative” menu, press Arrow Key to choose “Standard Curve”;

Press to go into sub menu.（Fig. 4-8）；Here you can create

a new curve, or you can load a saved curve. If you want to delete a
saved curve, just choose “Delete Curve”.

Fig. 4-8

(2) Set Wavelength

★ Move the cursor on “Create Curve”, the press to go into

the sample quantity setting interface. (Fig. 4-9)

Fig. 4-9

★ Press to go into the wavelength setting interface，input the

WL. Value by the Arrow Keypad and press to confirm.（Fig.

4-10）
；

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 Fig. 4-10
★ Use the Arrow Key to input the quantity of the standard samples,

then press to confirm. The system will go into the

following interface. (Fig. 4-11)

Fig. 4-11
(3) Calibrate 100%T/0Abs

Put the Reference Solution in the light path，then press to

calibrate 100%T/0Abs；

(4) Input Standard Samples’ Concentration

★ Pull the 1# Standard Sample in the light path and input the value of

its concentration by the Arrow keypad, then press to confirm.

The system will go into the next interface.

Fig. 4-12
★ Pull the 2# Standard Sample in the light path and input its

concentration, then press to confirm.

★ Repeat the above step till the last sample’s concentration is

finished, press to confirm. Then the Standard Curve and its

equation will display on the screen automatically. (Fig. 4-13) At the

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 same time, the Curve Equation will be saved in the RAM
automatically.

Fig. 4-13
Note: If there’s some mistake during the operation, the system will beep
3 times and return the initial interface automatically. Of course, the
curve can not be displayed on the screen.
(5) Measure Samples

★ Pull the Reference solution in the light path and press to

go into the continuous measurement interface. (Fig. 4-14)

Fig. 4-14

★ Pull the unknown sample in the light path, then press to

measure. The result will be displayed on the screen one by one. At
the same time, the data will be saved in the RAM automatically. Total
200 groups of data can be saved.

(6) Load Curve

All the curve equations will be saved in the RAM automatically. If you
want to reload the saved curve, just follow the following steps:

★ Move the cursor on “Load Curve” and press to confirm.

Then the system will go into the curve equation record interface.(Fig.
4-15)

Fig. 4-15

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★ Move the cursor on the equation that you need and press to
confirm. Then the corresponding curve will display on the screen.
(Fig. 4-16)

Fig. 4-16

★ Press to go into sample continuous measure interface and

measure samples. (Fig. 4-14)
Note: When you reload the curves, the wavelength will be set at the
point where you creat the Standard Curve automatically.
(7) Delete Curve

★ Move the cursor on “Delete Curve” and press to confirm.

Then the system goes into the curve equation choosing interface. (Fig.
4-17)

Fig. 4-17
★ Move the cursor on the curve equation that you don’t need again

and press , then system will ask you to confirm again.(Fig.

4-18)

Fig. 4-18

★ Move the cursor on “Yes” and press to confirm, then the

curve equation will be deleted. If you don’t want to delete it, just

choose “NO” or press to return.

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II Coefficient Method：
(1) Go into “Coefficient” method.

Press Arrow Key to choose “Coefficient”, press to go into

pre-test interface, then press to go into the parameter setting

interface.（Fig. 4-19）；

Fig. 4-19

(2) Parameter Setting

★ Use Arrow Key to move the cursor on “Coefficient K” and press

to go into K setting interface.（Fig. 4-20）；Input K and press

to confirm ， the system will return the former interface

automatically.

Fig. 4-20

★ Input the value of B in the same way, press to go back to

the pre-test interface. （Fig. 4-19）

(3) Set Wavelength

Press to go into wavelength setting interface ， input the

wavelength value by the Arrow keypad and press to

confirm. (Fig. 4-21）

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 Fig. 4-21
(4) Calibrate 100%T/0Abs

Press to go into continuous measurement interface (Fig.

4-22). Pull the Reference Solution in the light path ， then press

to calibrate 100%T/0Abs；

Fig. 4-22
(5) Measurement

Place the samples to be measured in the light path, press to

measure，then the result will display on the screen . Repeat this step
to measure other samples. All the test results will be listed on the
screen and stored in the RAM automatically. They can be reload even
after a sudden power cut.

Step 4: Print the test result

In the continuous measurement interface, press to print the test

result.

Step 5: Delete the test data

In the continuous measurement interface, press to delete the test

result.

4.3 SETUP

In the main menu, press Arrow Key to choose “Setup” and press to go
into utility setting interface（Fig. 4-23）

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 Fig. 4-23

1) Power on/off D2 lamp

When the wavelength point needed is in the range of

340-1100nm ， the D2 lamp can be switched off to prolong its
life time.

Use Arrow key to choose “D2 Lamp” and press to go into D2 lamp
setting interface（Fig. 4-24）. Press Arrow key to choose “On ” or “Off” and

press to confirm. Press to return.

Fig. 4-24

2) Power on/off W Lamp

When the wavelength point needed is in the range of

190-339nm, the W lamp can be switched off to prolong its life
time.

Use the key of Arrow Key to choose “W Lamp”，press to go into

W lamp setting interface（Fig. 4-25）. Use the key of Arrow Key to choose

“On” or “Off” and press to confirm. Press to return.

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 Fig. 4-25

3) Set Date & Time

Press Arrow Key to choose “Date & Time” and press to go into
setting interface.（Fig. 4-26）Use Arrow Key to choose setting item and
input the value by Arrow keypad.（Hour：0-23，Minute：0-59，Second：0-59，

Year：0000-9999，Month：1-12，Day：1-31, Week: 1-7），press to

return.

Fig. 4-26

4) Calibrate Dark Current

When the working condition changes, A dark current

calibration is required before any measurement.

Use Arrow key to choose “Dark Current” and press to confirm. (Fig. 4-27）
Note: Remove the cuvettes from the light path before this action, don’t
open the lid of the compartment during the course of calibration.

Press to return when it finishes.

Fig. 4-27

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5) Wavelength Calibration

After some time, the energy of lights would drop off and there
will be some but slight influence on the test result. In this case,
users can reset the wavelength to compensate. We suggest the
users to reset the wavelength every one or two months.

Remove all the blocks in the light path and close the lid of the compartment,

press Arrow Key to choose “Calibrate WL.” and press to begin the
calibration（Fig. 4-28）. The lid of the compartment should not be opened
during the calibration. It returns automatically after calibration.

Fig. 4-28

6) Lamps Change (Input the lamps changing wavelength point)

The instrument permits the users to set the lamps changing

wavelength point. In the range of 300-400nm, users can
choose the lamp freely.

Press Arrow Key to choose “Lamp Change” and press to go into

corresponding setting interface (Fig.4-29). Input the value of lamps changing

wavelength point by the Arrow keypad（300—400nm），then press to

confirm and return.

Fig. 4-29

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7) Load Default

Press Arrow Key to choose “Load Default” and press to confirm.（Fig.

4-30）， press Arrow Key to choose “Yes” and press to confirm.

Then the system will recover the default setting when leave factory. Choose

“No” with pressed to cancel the operation and return.

Fig. 4-30

8) Version

Press Arrow Key to choose “Version” and press to confirm. You’ll

see the version of the software and hardware. (Fig.4-31). Press to

return.

Fig. 4-31

Note: For the reason of update, the version you see on the screen may be
different from the above one.

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 CHAPTER 5 Instrument Maintenance

To keep the instrument work in good condition, constant maintain is needed.

1. Daily Maintain
1) Check the compartment
After measurement, the cuvettes with sample solutions should be taken out of the
compartment in time. Or the volatilization of the solution would make the mirror
go moldy. Users must pay more attention to the corrosive sample and liquid easy
to volatilize. Any solution remains in the compartment should be wiped off
immediately.

2) Surface Clean
The cover of the instrument is with paint. Please use wet towel to wipe off the
drip on the surface immediately. Organic solution is forbidden to be used to clean
the cover. Please wipe off the dirt on the cover timely.

3) Clean the cuvettes

After every test or after a solution change, the cuvettes should be cleaned carefully,
or the remains on the surface would cause measuring error.

2. Troubleshooting
1) Dark Current Error when self-test
Possible Cause Solution
 Open the lid of the compartment Close the lid of the compartment
during the course of self-test. and switch on the power again.

2) No response after power on

Possible Cause Solution
 Bad contact in power supply Improve the contact
 Fuse melt Replace a new fuse

3) Printer not work, printer error

Possible Cause Solution
No power supply Switch on the power supply
Bad contact in power supply Improve the contact
Bad contact of the data cable Improve the contact

4) No Stable Reading
Possible Cause Solution
 No enough pre-warm Increase the pre-warm time

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  Glass cuvettes used in UV Range Use Silicon Cuvettes.
 No stable Sample Improve the sample
 Much higher sample concentration Dilute the sample
 Low voltage or unstable power supply Improve the power condition
 Lights defect Replace a new lamp
 Light used up Replace a new lamp

5) Worse Repeatability
Possible Reason Solution
 Unstable sample Improve the sample
 Cuvettes polluted Clean the cuvettes

6) Incorrect reading
Possible Reason Solution
 Dark Current Error Re-get the dark current
 Worse matching of the cuvettes Improve the matching of the cuvettes

3. Spare part replacement

Replace the Fuse

Danger! Be sure to switch off the power and unplug the socket
before replacement!

Step 1: Tools preparation

Prepare a 3×75 Flat Blade screwdriver
Step2：Switch Off the power supply
Switch off the power supply, and unplug the socket.
Step 3: Take out the Fuse Seat
Take out the fuse seat by the screwdriver. (Fig.5-1)

Fig. 5-1

Step 4: Replace a new fuse

Pick out the spare fuse and replace it to the working position. (Fig.5-2)

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 Fig. 5-2

Step 5: Reset the fuse seat

Replace the fuse seat in the power socket
Step 6: Switch on the power
Plug the socket and switch on the power

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Drawell International Technology Limited
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1506,Lane581 XiuChuan Rd.,PuDong New Area,Shanghai,China
Tel: 0086 21 54411195
Fax: 0086 21 33823261
Web : www.drawell.com.cn
Email : [email protected]

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