DAVAO CENTRAL COLLEGE

Juan dela Cruz St., Toril, Davao City, Philippines

ACSCU-ACI ACCREDITED
College of Education and Liberal Arts

S BIO 3 Microbiology and Parasitology

PRELIM EXAMINATION
Prepared By: Jay Ar A. Robles, LPT

Name: Date:
Year/Course: Score:

I. Multiple Choice
Write the CAPITAL LETTER of your choice on the space provided before every item. (1 point each)
______ 1. He resolve the concept of biogenesis, the claim that living cells can arise only from preexisting living
cells in 1861.
A. Rudolf Virchow
B. Louis Pasteur
C. Hans Christian Gram
D. John Tyndall

______ 2. The first to observe live microorganisms from his own feces and semen.
A. Robert Hooke
B. Anton van Leeuwenhoek
C. Zacharias Janssen
D. Ernst Karl Abbe

______ 3. Designed to suppress the growth of unwanted bacteria and encourage the growth of the desired
microbes.
A. Differential Media
B. Selective Media
C. Reducing Media
D. Chemically Defined Media

______ 4. The ability of the Microscope lenses to distinguish fine detail and structure.
A. Brightfield illumination
B. Resolution
C. Refractive Index
D. Objectives

______ 5. All of the statements below describe Cellular Respiration EXCEPT.

I. An ATP-generating process in which molecules are oxidized.

II. The final electron acceptor is (almost always) an inorganic molecule.
III. Do not need a NAD+ and NADP+ in the process.
IV. 1 type of respiration, depending on whether an organism is an aerobe, which uses oxygen, or an anaerobe.

A. I & II
B. I & III
C. I & IV
D. I only

______ 6. Using microbes to clean up pollutants and toxic wastes produced by various industrial processes.
A. Microbial Ecology
B. Bioremediation
C. Biofilms
D. Vaccination
______ 7. The removal of electrons (e−) from an atom or molecule, a reaction that often produces energy.
A. Oxidation
B. Reduction
C. Oxidation-reduction Reaction
D. Dehydrogenation

______ 8. The study of fungi.

A. Immunology
B. Virology
C. Parasitology
D. Mycology

______ 9. The two of the most important coenzymes in cellular metabolism.

A. Flavin Mononucleotide (FMN) and Flavin Adenine Dinucleotide (FAD)
B. Nicotinamide Adenine Dinucleotide (NAD+) and Adenosine Triphosphate (ATP)
C. Nicotinamide Adenine Dinucleotide Phosphate (NADP+) and Adenosine Triphosphate (ATP)
D. Nicotinamide Adenine Dinucleotide (NAD+) and Nicotinamide Adenine Dinucleotide Phosphate (NADP+)

______ 10. Factors influence the activity of an Enzyme.

I. Temperature
II. pH
III. Substrate concentration
IV. The presence or absence of inhibitors

A. I only
B. I & II
C. I, II, III
D. I, II, III, IV

______ 11. It means coloring the with a dye that emphasizes certain structures.
A. Fixing
B. Staining
C. Smearing
D. Dying

______ 12. The cell walls of this bacteria consist of one or a very few layers of peptidoglycan and an outer
membrane.
A. Gram-negative Bacteria
B. Gram-positive Bacteria
C. Gram-negative Archaea
D. Gram-positive Archaea

______ 13. Arrange the following Staining Procedure in order in classifying gram-positive and gram-negative
bacteria.
I. Alcohol wash (decolorization)
II. Application of crystal violet (purple dye)
III. Application of iodine (mordant)
IV. Application of safranin (counterstain)

A. I-II-III-IV
B. I-II-IV-III
C. II-III-I-IV
D. II-I-III-IV
______ 14. The stains that are most frequently used for bacteria are the Gram stain and the acid-fast stain.
A. Acid-fast Stains
B. Differential Stains
C. Simple Stains
D. Negative Stains

______ 15. It using practices and procedures to prevent contamination from microorganism.
A. Vaccination
B. Chemotherapy
C. Aseptic Technique
D. Antibiotics

______ 16. The statement/s that describes Catabolic reactions.

I. The energy transferred by the particles in the collision

II. Provide building blocks for anabolic reactions
III. Explains how certain factors affect the rates of those reactions
IV. Furnish the energy needed to drive anabolic reactions.

A. I & II
B. I & IV
C. II & III
D. II & IV

______ 17. It is used to examine live microorganisms that cannot be stained by standard methods, or are so
distorted by staining that their characteristics then cannot be identified.
A. Darkfield Microscope
B. Phase-contrast Microscope
C. Electron Microscope
D. Compound Microscope

______ 18. The nonprotein component of an enzyme.

A. Coenzyme
B. Apoenzyme
C. Cofactor
D. Holoenzyme

______ 19. It basically consists of interpreting the action of a sound wave sent through a specimen.
A. Differential Interference Contrast (DIC) Microscopy
B. Scanning Acoustic Microscopy
C. Confocal Microscopy
D. Fluorescence Microscopy

______ 20. A technique in light microscopy used to reconstruct three-dimensional images.

A. Differential Interference Contrast (DIC) Microscopy
B. Scanning Acoustic Microscopy
C. Confocal Microscopy
D. Fluorescence Microscopy
II. Identification
A. Identify the Group of Microorganism described by the following statements. (1 point each)
Bacteria Archaea Fungi Protozoa Algae Viruses

_____________1. May be unicellular or multicellular that is look somewhat like plants, but cannot carry
out photosynthesis.

_____________2. They have cell walls that lack peptidoglycan.

_____________3. Microorganisms categorize as Prokaryotes.

_____________4. They are photosynthetic eukaryotes with a wide variety of shapes and both sexual
and asexual reproductive forms.

_____________5. They are enclosed in cell walls that are largely composed of a carbohydrate and
protein complex called peptidoglycan.

_____________6. There are generally reproduce by dividing into two equal cells; this process is called
binary fission.

_____________7. Their particle contains a core made of only one type of nucleic acid, either DNA or
RNA.

_____________8. They are often found in extreme environments.

_____________9. They are acellular and their size is so small that most can be seen only with an
electron microscope.

_____________10. They are unicellular eukaryotic microbes that have pseudopods, flagella, or cilia for
locomotion.

B. Identify the Parts/Organelles of a Microbial Cell described by the following statements.

(2 points each)

_____________1. A sticky outer protective covering found in the microbial cells, in addition to the cell
wall. Usually made of polysaccharide.

_____________2. The gel-like fluid inside the cell where the genetic materials and organelles of a
microbial cell is found.

_____________3. The sites of protein synthesis.

_____________4. A long filamentous appendages used by microbes of movement.

_____________5. The site where the synthesis of phospholipids, fats and steroids (estrogens and
testosterone) taking place.

_____________6. Special extension of bacterial cell which are made for the transfer of DNA to other
microbial cell.

_____________7. A space or cavity in the cytoplasm of a cell that is enclosed by a membrane called a
tonoplast function as the temporary storage organelles for substances such as water,
proteins, sugars, organic acids, and inorganic ions.
_____________8. A short, hair-like structures that cover the surface of microbial cells in large numbers
that is used for movement and gather information about their environment.

_____________9. A membrane-enclosed structure that contains both the pigment chlorophyll and the
enzymes required for the light-gathering phases of photosynthesis

_____________10. It contains a single long, continuous, and frequently circularly arranged thread of
double-stranded DNA called the bacterial chromosome.

III. Discussion
1. What are the relationships between the Normal Microbiota and the Host? (5 points)

IV. Bonus
1. DCC was burnt to ashes in two massive fires. Provide the complete date of one of the fire incidents?
[Month, Day, Year] (3 points)

"Go forth and set the WORLD ON FIRE!"

St. Ignatius of Loyola
† Ad Majorem dei Gloriam †