0% found this document useful (0 votes)
38 views5 pages

Molecular Research on Guinea Fowl Genetics

The document discusses the significance of molecular research in addressing various scientific and agricultural challenges, particularly focusing on guinea fowl. It highlights several studies on guinea fowl, including molecular sexing, taxonomic classification, and MHC class II region characterization, revealing insights into their genetic diversity and evolution. The findings underscore the importance of molecular techniques in improving breeding practices and conserving genetic resources in guinea fowl populations.

Uploaded by

Awe
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
0% found this document useful (0 votes)
38 views5 pages

Molecular Research on Guinea Fowl Genetics

The document discusses the significance of molecular research in addressing various scientific and agricultural challenges, particularly focusing on guinea fowl. It highlights several studies on guinea fowl, including molecular sexing, taxonomic classification, and MHC class II region characterization, revealing insights into their genetic diversity and evolution. The findings underscore the importance of molecular techniques in improving breeding practices and conserving genetic resources in guinea fowl populations.

Uploaded by

Awe
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

INTRODUCTION

Molecular Research have tremendous value not only in the investigation of basic scientific questions,
but also in application to a wide variety of problems affecting farm animals and the overall human
condition. Disease prevention and treatment, generation of new protein products, and manipulation
of plants and animals for desired phenotypic traits are all applications that are routinely addressed
by the application of molecular researches. Because of the wide applicability of these methods, they
are rapidly becoming a pervasive - some would argue invasive - aspect of our technologically based
society. The public concerns that address the application of these methods should be addressed by
informed public discussion and debate.

The following are few of the available molecular research works that were conducted on guinea
fowl.

1. Molecular Sexing and Taxonomic classification of Nigerian Guinea Fowl using Chromo
Helicase DNA Binding Gene and 12S mitochondrial rRNA gene
Sola-Ojo, F.E1*., Afolabi-Balogun, N.B2 ., Adeniyi C.A3 ., Adeyemi, K.D1 ., Ayorinde, K.L1 ., Alli, O.I1 ., Oni,
O.A2 ., Okeke, C.U4 ., Momoh E.O1 ., Adewara, J1 ., and Abdulkareem, I1 . 1 Quantitative, Molecular and
Functional Genomics Unit, Department of Animal Production, University of Ilorin, Ilorin, Kwara State, Nigeria. 2
Molecular Biology and Genetic Diversity Research Laboratory, Biochemistry Unit, Department of Chemical
Sciences, and Fountain University Osogbo, Osun State, Nigeria. 3 State Key Laboratory of Genetic Resources
and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China. 4 Department of
Prosthetics and Orthotics, School of Health Technology Owerri, Imo State, Nigeria.

A short background

Guinea fowl are birds derived from the African Guinea coast and are one of the economically
important bird originating from the southern part of the Sahara and majorly in West Africa and it is
widely domesticated in the region (Crawford, 1990). It is domesticated from wild helmeted guinea
fowl (Numida meleagris) and accounts for 3% of global poultry breeds (FAO, 2007). Apart from being
a valued source of meat, eggs and feathers, domesticated guinea fowl has value for pest control and
alerting intruders (Ayeni, 1983; Moreki and Seabo, 2012). Despite the economic importance of
guinea fowl, the extent of its genetic diversity remains an enigma (Adeola et al., 2015).

In Nigeria, Guinea fowl is one of the indigenous poultry and also an integral part of rural poultry. A
recent report indicated that it is the second most widely domesticated poultry after chicken (NBS,
2012) and found mainly in northern Nigeria. In spite of the abundance

THE RESEARCH IN SUMMARY

Sexing of birds at early age is very important for efficient selection and breeding; while
characterization and taxonomic identification is relevant in conservation of birds’ genetic resources.
This study used the genomic DNA of ten (10) guinea fowl keets to determine their sex using agarose
gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, they were also
characterize taxonomically using 12S rRNA mitochondria genes. The results of this study shows a
double band (ZW) for females and a single band (W) for males under Agarose gel electrophoresis
view, the Guinea fowl keets sequenced showed some deletions and were closer to Gallus_CHD12 in
the phylogenetic tree. The Taxonomic classification result shows that the sequenced guineafowl
keets were most related to the Numida meleagris 12S mitochondrial ribosomal RNA. This study
corroborate the fact sex of guineafowl keet can be easily identified at genomic DNA level and they
can be characterized taxonomically using the 12SrRNA mitochondrial genes
2. Molecular characterization of MHC class II region in guinea
Fowl

S K Singh 1, J Mathew, J Gupta, S Mehra, G Goyal, D Sharma

1. The MHC class II gene was amplified, cloned and sequenced in guinea fowl.

2. The NumeMHC II sequence of 754 nucleotides included complete exon 1 (91 nt), exon 2
(270 nt), exon 3 (282 nt) and exon 4 (110 nt).

3. The size of β(1) and β(2), domains were 89 and 93 amino acids, respectively in guinea
fowl.

4. High amino acid variability (38·2%) was observed within guinea fowl in β(1) domain,
while in β(2) domain, amino acid variability (6·3%) was low.

5. Among poultry species, the percent amino acid identity between guinea fowl and chicken,
quail, pheasant and duck was 38·8, 42·2, 44·4 and 58·8 in β(1) domain; and 13·8, 17·0, 13·8
and 27·6 in β(2) domain, respectively.

6. Sequence alignment with mammalian and avian MHC showed that many of the conserved
features of MHC class II glycoprotein was conserved in guinea fowl.

7. Within-species genetic distances (Poisson correction) based on cumulative amino acid


variability in β(1) domain and β(2) domains was 0·141 in guinea fowl.

8. Guinea fowl showed low and similar genetic distances with all the poultry species (0·255-
0·268) except duck (0·456).

9. Guinea fowl made separate branch within the major cluster having chicken, quail and
pheasant, showing equal distance from these poultry species, whereas duck MHC II clustered
separately.

3. A guinea fowl genome assembly provides new evidence on


evolution following domestication and selection in galliformes
Alain Vignal 1, Simon Boitard 1, Noémie Thébault 1, Guiguigbaza-Kossigan Dayo 2, Valentine Yapi-
Gnaore 2, Issaka Youssao Abdou Karim 3, Cécile Berthouly-Salazar 4 5, Nóra Pálinkás-
Bodzsár 6, Daniel Guémené 7, Francoise Thibaud-Nissen 8, Wesley C Warren 9 10, Michèle Tixier-
Boichard 11, Xavier Rognon 11

The helmeted guinea fowl Numida meleagris belongs to the order Galliformes. Its natural range
includes a large part of sub-Saharan Africa, from Senegal to Eritrea and from Chad to South
Africa. Archaeozoological and artistic evidence suggest domestication of this species may have
occurred about 2,000 years BP in Mali and Sudan primarily as a food resource, although
villagers also benefit from its capacity to give loud alarm calls in case of danger, of its ability to
consume parasites such as ticks and to hunt snakes, thus suggesting its domestication may
have resulted from a commensal association process. Today, it is still farmed in Africa, mainly
as a traditional village poultry, and is also bred more intensively in other countries, mainly
France and Italy. The lack of available molecular genetic markers has limited the genetic studies
conducted to date on guinea fowl. We present here a first-generation whole-genome
sequence draft assembly used as a reference for a study by a Pool-seq approach of wild and
domestic populations from Europe and Africa. We show that the domestic populations share a
higher genetic similarity between each other than they do to wild populations living in the same
geographical area. Several genomic regions showing selection signatures putatively related to
domestication or importation to Europe were detected, containing candidate genes, most
notably EDNRB2, possibly explaining losses in plumage coloration phenotypes in domesticated
populations.
REFERENCES

Adeola, A.C., Ommeh, S.C., Murphy, R.W., Wu, S.F., Peng, M.S., Zhang, Y.P. (2015). Mitochondrial
DNA variation of Nigerian domestic helmeted guinea fowl. Animal Genetics. 2015 Oct ; 46 (5):576-9.
doi: 10.1111/age.12324. E pub 2015 Jul 7. PMID: 26153528. Awotwi, E. K. (1975). Some aspects of
the reproductive physiology of male and female guinea fowls Msc. Thesis. Ghana: University of
Ghana.

Ayeni J.S.O. (1983) Studies of grey breasted helmet guinea fowl (Numida meleagris galeata Pallas) in
Nigeria. World Poultry Science Journal 59, 143–51. Cerit, H. and Avanus, K. (2007). Sex identification
in avian species using DNA typing methods. Journal of World's Poultry Science 63, 91-100.

Chen, W., He, B., Li, C., Zhang, X., Wu, W., Yin, X., Fan B., Wang, J. (2007). Real-time RT-PCR for H5N1
avian influenza a virus detection. Journal of Medical Microbiology, 56:603-607.

Clinton, M., Haines, L., Belloir, B., Mcbride, D. (2001) Sexing chick embryos: a rapid and simple
protocol. British Poultry Science 42, 134-138.

Crawford R.D. (1990) Poultry biology: origin and history of poultry species. In: Poultry Breeding and
Genetics (Ed. by R.D. Crawford), pp. 1–42. Elsevier Science Publishing Company, Amsterdam and
New York, NY.

Dubiec M. and Zagalska-Neubauer (2006). Molecular techniques for sex identification in birds,
Biological Lett. 2006, 43(1): 312.

Delgado, J. V., Barba, C., Camacho, M. E., Sereno, F.T.P.S., Martinez, A. and Vega Pla, J. L. (2001).
Livestock characterisation in Spain. AGRI. 29:7-18.

Ellegren H. (1996). First gene on the avian W chromosome (CHD) provides a tag for universal sexing
of nonratite birds. Proc. R. Soc. B Biol. Sci., 263 (1996), pp. 1635-1641 FAO (2007). Fisheries Technical
Paper. No. 497. Rome, FAO. 2007. 510p. Study and analysis of feeds and fertilizers for sustainable
aquaculture development.

Gholidazeh, M., Mianji, G.R., Zadeh, H.S. (2008): Potential use of molecular markers in the genetic
improvement of livestock. Asian Journal of Animal and Veterinary Advances 3(3), 120-128.

Gizaw, S., Komen, H., Hannote, O., Van, A. J. A. M., Kemp, S., Haile, A., Mwai, O. and Dessie, T.
(2011). Characterization and conservation of indigenous sheep genetic resources: A practical
framework for developing countries. ILRI Research Report No 27. Nairobi, Kenya.

Griffiths, R., Double, M.C., Orr, K., Dawson, R.J. (1998). A DNA test to sex most birds. Molecular
Ecology, 7 (1998), pp. 1071-1075 Halima, H.M. (2007): Phenotypic and genetic characterization of
indigenous chicken populations in northwest Ethiopia, Ph. D. Thesis, University of the Free State,
Bloemfontein, South Africa, 175 pages.

Harvey, M.G., Bonter, D.N., Stenzler, L.M. & Lovette, I. J. (2006) A comparison of plucked feathers
versus blood samples as DNA sources for molecular sexing. Journal of Field Ornithology 77(2), 136-
140.

Kalina, J., Mucksová, J., Yan, H. & Trefil, P. (2012) Rapid sexing of selected Galliformes by Polymerase
Chain Reaction. Czech Journal of Animal Science 57 (4), 187- 192

Moreki J.C. and Seabo D. (2012) Guinea fowl production in Botswana. Journal of World’s Poultry
Research 2(1), 1–4.
AN INVESTIGATION INTO THE
MOLECULAR RESEARCH
WORKS DONE ON GUINEA
FOWL

A COMPILATION BY

AWE OLANREWAJU OLUWASEUN

UIL/ PG2020/086

DEPARTMENT OF ANIMAL PRODUCTION

UNIVERSITY OF ILORIN, NIGERIA.

December 19, 2022

Common questions

Powered by AI

Molecular research contributes to the improvement of poultry breeds, such as guinea fowl, by enabling identification of genetic diversity and sexing at an early stage. By using genomic techniques such as agarose gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, researchers can determine the sex of guinea fowl keets efficiently. This aids in selective breeding and conservation of genetic resources . Moreover, molecular research helps in elucidating genetic relationships and evolutions post-domestication, as observed in the genetic similarity studies between domestic and wild guinea fowl populations .

The advantages of using genomic DNA techniques for sexing guinea fowl include increased accuracy and efficiency over traditional methods, as they allow for early sex identification using chromo helicase DNA (CHD) binding genes and 12S rRNA mitochondrial genes. This enables more precise and quicker selection and breeding strategies, ultimately improving productivity. However, challenges may include the costs and technical expertise required for such molecular techniques, as well as the need for facilities capable of performing these analyses in less technologically advanced regions .

The application of molecular research techniques to animal breeding and conservation has broad societal implications, particularly in addressing public concerns regarding the invasiveness of these techniques. On one hand, it offers the potential for significant advancements in breeding efficiency and genetic conservation, ensuring sustainable practices in agriculture and food production. On the other hand, there are ethical considerations relating to genetic modification and biodiversity conservation that require informed public debate and policy-making to address potential risks and cultural impacts .

Identifying new genetic markers in guinea fowl is significant for future genetic studies and breeding programs as it provides critical tools for assessing genetic diversity, evolutionary relationships, and selection traits. These markers can facilitate the development of advanced breeding strategies to enhance desirable traits such as disease resistance, productivity, and adaptability to different environments. Furthermore, they can serve as benchmarks for tracking genetic changes over time, assisting in maintaining biodiversity and targeting conservation efforts effectively .

The genomic similarities between domesticated guinea fowl populations suggest that they might exhibit consistent traits prized across various global markets, such as meat quality or disease resistance. This genetic consistency can ease the global distribution and utilization of domesticated guinea fowl as it ensures expected performance across different farming systems and geographic conditions. However, it also poses a risk of reduced genetic resilience if confronted with widespread pathogens, which can be mitigated by fostering genetic diversity among breeding stocks .

High genetic similarity among domestic guinea fowl populations implies potential vulnerability to diseases and environmental changes due to reduced genetic diversity. This can lead to a lack of resilience against new or rapidly changing threats, necessitating focused genetic conservation efforts to introduce or preserve genetic variability. Conservation strategies could include the introduction of genetic material from wild or less related populations to enhance genetic diversity and adaptability, ensuring long-term sustainability of these bird populations .

The study on the taxonomic classification of guinea fowl using 12S rRNA mitochondrial genes provides insights into their evolutionary relationships by comparing their genomic sequences with those of other birds. The results indicate that guinea fowl have a close genetic relationship with Numida meleagris 12S mitochondrial ribosomal RNA and show phylogenetic closeness to birds like Gallus gallus. Such findings help clarify evolutionary lineages and evolutionary divergences among poultry, contributing to a broader understanding of avian biodiversity and evolutionary history .

Genetic diversity studies are pivotal in the conservation of guinea fowl populations as they provide insights into the genetic health and adaptability of these birds. Understanding the genetic variability allows conservationists to develop better-informed strategies that maintain or even increase diversity, thus enhancing the populations' resilience to environmental changes and diseases. Identifying genetic diversity also aids in recognizing distinct lineages, which can be crucial for preserving unique traits and ensuring sustainable utilization of guinea fowl as a resource .

The domestication process has influenced the genetic makeup of guinea fowl by selecting for traits beneficial to human cohabitation and farming needs. Domesticated populations of guinea fowl have shown higher genetic similarity among each other compared to their wild counterparts living in the same regions, which is indicative of selective breeding practices. Additionally, certain genomic regions associated with domestication, such as those affecting plumage coloration, have been identified, which may have been selectively bred for ease of management and aesthetic reasons .

Genetic variability in the MHC class II genes is crucial for guinea fowl production and health management as it influences the immune response diversity. High amino acid variability in the β(1) domain of the MHC class II gene indicates a potential for diverse immune response capabilities, which can enhance disease resistance in populations. Consequently, understanding this genetic variability can guide breeding programs aimed at improving disease resilience, thus reducing dependency on medical interventions and increasing overall poultry production efficiency .

You might also like