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neofleX TOF TOF_Spec Sheet_06-2024

The neofleX MALDI-TOF/TOF MS System is a benchtop mass spectrometer featuring ultrafast scanning and advanced imaging capabilities. It includes a BRUKER smartbeam™ 3D laser, a mass range of up to 500,000 m/z, and high mass accuracy and resolution for both linear and reflector modes. Designed for research use only, the system is equipped with a robust vacuum system and offers significant sensitivity for various applications.

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62 views

neofleX TOF TOF_Spec Sheet_06-2024

The neofleX MALDI-TOF/TOF MS System is a benchtop mass spectrometer featuring ultrafast scanning and advanced imaging capabilities. It includes a BRUKER smartbeam™ 3D laser, a mass range of up to 500,000 m/z, and high mass accuracy and resolution for both linear and reflector modes. Designed for research use only, the system is equipped with a robust vacuum system and offers significant sensitivity for various applications.

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AXIAL-TOF MS

neofleX MALDI-TOF/TOF MS System


Technical Specifications
Benchtop MALDI TOF/TOF Mass Spectrometer System with ultrafast scanning
Smartbeam 3D™ laser with up to 20 pixels per second at 20 µm, enhanced imaging
detectors in linear and reflector mode for imaging of MALDI HiPLEX-IHC and general
high-end MALDI applications.

Part Number 1907227

Technical Data
Size (L×W×H) Bench top:
▪ 157 cm × 71 cm × 73cm
▪ 62 in × 28 in × 29 in

Weight ▪ 270 kg / 595 lb

Vacuum System ▪ Turbomolecular pump with oil free pre-vacuum pump

MALDI Ion Source ▪ scoutMTP – microtiter plate format


▪ Self-aligning lens-pack for maximum uptime and easy maintenance
▪ Wrench-free source cleaning
▪ Ion acceleration up to ±20 kV

Laser ▪ BRUKER scanning smartbeam™ 3D laser, 355 nm wavelength


▪ Enabling True Pixel MALDI Imaging
▪ Minimum number of 1.0∙1010 laser shots
▪ Repetition rate up to 10 kHz

Ionization mode ▪ Positive and Negative Ion Modes

Ion Optics Effective Flight Length


▪ 124 cm Linear Mode
▪ 260 cm Reflector Mode

Acquisition Workstation hp Z4 / Win10 / FlexCompass 2025

System Certification CE, ETL


Level
Analytical Performance
Mass Range Up to 500,000 m/z

Mass Accuracy Linear Mode Reflector Mode


≤ 50 ppm with external calibrant ≤ 6 ppm with external calibrant
≤ 60 ppm with internal calibrant ≤ 1.5 ppm with internal calibrant

Resolving Power Linear Mode (Positive) Reflector Mode (Positive)


(FWHM) ≥ 5,000 @ m/z 2,465 (ACTH_clip [1-24]) ≥ 30,000 @ m/z 3,147 (Somatostatin)
Linear Mode (Negative) Reflector Mode (Negative)
≥ 4,000 @ m/z 2,465 (ACTH_clip [1-24]) ≥ 15,000 @ m/z 1,568 (Glu-Fib)

Mass Resolution* Linear Mode (Protein*) Reflector Mode (Peptide*)


(Protein/Peptide) ≥ 700 for Insulin (m/z 5,734) ≥ 12,000 for Bradykinin [2-9] (m/z 904)
≥ 1,000 for Myoglobin M2+ (m/z 8,476) ≥ 14,000 for ACTH_clip [1-17] (m/z 2,093)
≥ 1,200 for Cytochrome C (m/z 12,361) ≥ 20,000 for ACTH_clip [1-24]) (m/z 2,932)
≥ 1,100 for Myoglobin (m/z 16,952) ≥ 25,000 for ACTH_clip [7-38]) (m/z 3,657)
≥ 25,000 for ACTH_clip [1-39] (m/z 4,539)

Sensitivity Linear Mode Reflector Mode


▪ BSA (m/z 66,431) @ S/N ≥ 100:1 for ▪ [Glu1]-Fibrinopeptide B (m/z 1,570) @
< 500 fmol shown with 1,000 laser shots S/N ≥ 200:1 for 250 amol shown on
Bruker AnchorChip™ target with 2,000
laser shots

Imaging Up to 20 px/s at 20µm spatial resolution with enhanced imaging detectors.

TOF/TOF Acquisition
Max. usable laser speed 5000 Hz

MS/MS Sensitivity [Glu1]-Fib fragment (m/z 1,056) @ S/N ≥ 40:1 for 250 amol shown on Bruker
AnchorChip™ target with 2,000 laser shots

MS/MS Mass Accuracy Average Mass Error ≤ 0.025 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z
1,570)

MS/MS fragment ≥ 1,500 for [Glu1]-Fib fragment m/z 175


resolution ≥ 3,000 for [Glu1]-Fib fragment m/z 684
≥ 5,000 for [Glu1]-Fib fragment m/z 1,056
≥ 7,000 for [Glu1]-Fib fragment m/z 1,441

Pre Cursor Ion Selector Precursor ion selection has a resolution of ≥ 500 FWHM
Resolution

High-energy CID-Method The following d-ions will appear in 1 pmol Substance P (m/z 1,347) spectra

Fragment d3(K) d5(Q) d6(Q) d10(L)

Mass 297.3 Da 522.4 Da 650.4 Da 1,129.6 Da

S/N of d6-fragment ≥ 30:1

* Mass resolution over broad mass range measured simultaneously with PAN™ technology.

RUO: For Research Use Only. Not for use in clinical diagnostic procedures.
We are continually improving products and reserve the right to change specifications without notice.
© Bruker Daltonics GmbH & Co. KG 06–2024

Bruker Daltonics GmbH & Co. KG Bruker Scientific LLC Online information
Bremen · Germany Billerica, MA · USA bruker.com
Phone +49 (0) 421-2205-0 Phone +1 (978) 663-3660

[email protected] – www.bruker.com

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