Power Pac HV BioRad
Power Pac HV BioRad
Power Supply
Instruction Manual
Catalog Numbers
164-5056
and
164-5059
Technical Support
For Technical Support contact your local Bio-Rad office. In the US call 1-800-424-6723.
Please have the following information available.
Model number: Located on the sticker on the bottom of the unit.
Serial number: Located on the sticker on the bottom of the unit.
Firmware version: The PowerPac HV displays the software version momentarily after
switching the power ON.
State clearly the error code, error message or anomaly, and the conditions that originated
the problem, including run parameters (volts, amperes and watts) as well as electrophoresis
cell and buffer system.
Calibration and Service agreements are available through Technical Support.
! ! Caution
!
This product conforms to the Class A Standards for electromagnetic emissions, intended
for laboratory equipment applications. It is possible that emissions from this product may
interfere with some sensitive appliances when placed near or on the same circuit. The user
should be aware of this potential and take appropriate measures to avoid interference.
*The EN 61010 is an internationally accepted electrical safety standard for laboratory instruments.
Table of Contents
Page
Section 1
Introduction
1.1 Overview
The PowerPac HV power supply is designed to provide constant voltage, current, or power
for a wide range of electrophoresis applications, including IEF and SDS PAGE. It also
provides temperature control for DNA sequencing with the Sequi-gen cell.
The PowerPac HV operates at the constant value specified by the user. If a non-constant
parameter becomes limiting, the PowerPac HV will automatically cross over and continue
operating at the limiting parameter.
Output range
Voltage 20–5,000 Volts (V)
Adjustable in 0.1 V increments from 20.0 to 99.9 V
Adjustable in 1 V increments from 100 to 5000 V
Current 0.05–500 mA.
Adjustable in 0.01 mA steps from 0.05 to 9.99 mA
Adjustable in 1 mA increments from 10 to 500 mA
Power 0.1–400 W
Adjustable in 0.1 Watt increments from 0.1 to 9.9 W
Adjustable in 1 W increments from 10 to 400 W
Output jacks Four sets of output jacks are provided to facilitate connection of up
to four identical electrophoresis cells simultaneously
1 2 abc 3 def
EDIT 0 CE
FRONT PANEL
OUTPUT JACKS
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PowerPac HV – Instruction Manual
1.2 Features
The PowerPac HV has the following features:
• Constant voltage, voltage ramping, constant current or constant power operation with
automatic crossover and temperature control mode
• Backlit LCD screen
• Continuous display of all parameters
• Continuous, timed and Volthours run control
• Nine (9) programmed runs, each containing up to nine steps
• Pause mode for editing running parameters
• Automatic detection of no-load, short circuit, rapid resistance change, ground leak, fan
failure and system overheating
• Automatic completion (if desired) of a run interrupted by AC power failure
• Stackable case with adjustable viewing angle (Figure 3)
• Adjustable LCD display contrast
• EN 61010 international safety certification
• Input power 100–120/220–240 VAC, 50/60 Hz, auto-switching
• Four output terminals
1 2 abc 3 def
EDIT 0 CE
LEGS
(LOWERED POSITION)
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PowerPac HV – Instruction Manual
1.3 Unpacking
When the power supply is received, carefully inspect the container for any damage which
may have occurred in shipping. Severe damage to the container may indicate damage to the
power supply itself. If you suspect damage to the unit, immediately file a claim with the carrier
in accordance with their instructions before contacting Bio-Rad Laboratories.
Unpack the PowerPac HV. Remove the plastic film from the translucent green top case.
The plastic film may leave a residue. If so, clean with a soft, damp cloth.
Contents include:
• PowerPac HV power supply
• Power cord
• Instruction manual
• Warranty card
• Declaration of conformity
If any part is missing or damaged, contact Bio-Rad Laboratories immediately.
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PowerPac HV – Instruction Manual
Section 2
Control Panel
1 2 abc 3 def
SETUP KEY
IR PORT
FUNCTION KEYS
EDIT KEY CURSOR KEYS
Key Description
Run/Pause Starts or pauses a run. When paused, the run parameters for the
current or subsequent step(s) may be edited. The modified method may
be stored to permanent memory by pressing SAVE at the end of the run.
Stop/Home Terminates the run in progress and displays the final run parameters, or
if no run is active, changes the display to the Home screen.
Note: If a method has been edited during a run, SAVE must be pressed
at the end of the run to save the changes.
Setup The SETUP menu allows the operator to set preferences. Press Setup
key again to exit the SETUP menu.
Preferences include: power failure detection, rapid resistance change
detection, no load detection, IR Data acquisition, contrast, key sound,
temperature probe check date and time settings (see Section 3.2).
EDIT Key Toggles soft key assignments between those used to set the run mode
(constant voltage, constant current, or constant power), run
EDIT
limits (voltage, current, or power) and time mode (hours, volt-hours, or
untimed).
Up & down Used to scroll through method list or method protocol. An asterisk (*) is
arrows and used to identify the selected method or step. The up arrow is used to
decimal point enter decimal point during parameter programming.
Alphanumeric Used to enter parameter values and method names. When method
keypad names are entered, the manner in which keys are pressed determines
the characters entered and their placement. Rapid repetitive strokes on a
single key, toggles the character displayed at the cursor position. The
cursor position advances each time a different key is pressed or when
there is a pause between strokes of a single key. The “0” key accesses
the “pH” and hyphen “–” sequence.
Function or The function of the three soft keys is indicated by the legend displayed
Soft keys directly above the key. This will vary with the menu tree.
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PowerPac HV – Instruction Manual
Section 3
Product Setup
3.1 Power Supply Connections
This section describes how the PowerPac HV power supply is connected to an electrophoresis
cell or cells.
Step Procedure Description
1. Connect the Connect one end of the included power cord to the
power cord rear of the instrument. Connect the other end to a
compatible power source.
2. Connect cells Insert power leads into one of the output terminals
located on the front of the power supply (shown to
the left). Note that the symbol indicates high
voltages and that the
power leads must be inserted perpendicular to the
curve of the case (see below).
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PowerPac HV – Instruction Manual
Step Procedure Description
3. Turn power on Use the switch located on the right side of the power
supply to power on. Initially, the unit will display the
firmware version and serial number for a few seconds,
then go to the Home screen. The Home screen soft
keys are used to select operation modes; Manual
(Section 4.1), IEF Methods (Section 4.3), and Basic
Methods (Section 4.2).
2. Select the SETUP Scroll through the SETUP preferences using the
preference arrow keys. The asterisk indicates the selected
preference.
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PowerPac HV – Instruction Manual
3.3 Explanation of Safety Features and Setup Options
3.3.1 Pfd: Power Failure Detection
Preference Options Description
PFd OFF OFF: Turns power failure detection OFF.
Power Failure Detection (Default) In this mode, the run is terminated if a
NEXT RUN power failure occurs.
ON
NEXT RUN: Turns power failure
SETUP detection on for a single run. PFd will
* PFd OFF show on the upper right corner of the
RRCd ON
Run screen.
NLd ON
CHANGE EXIT RESET ON: Turns power failure detection mode
on in all runs. If there is a power failure in
this mode, then the run will resume when
the power is restored. PFd will show on
the upper right corner of the Run screen.
Warning: Turning the power supply off
(Stopping a run in progress with PFd
enabled, is regarded as a power failure.)
The "interrupted" run will resume
automatically when the power is
restored.
1. If a power failure occurred during the run with
PFd enabled, the MORE soft key is displayed
on the Run Completed screen.
Press MORE to display details about the most
recent power outage and when power was
restored.
Press RERUN to restart the run.
Press BACK to return to the Run Completed
screen. Press EXIT to go to the Method screen.
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PowerPac HV – Instruction Manual
3.3.2 RRCD: Rapid Resistance Change Detection
Preference Options Description
RRCd ON ON: Detection of a sudden resistance
Rapid Resistance Change (Default) change greater than 20% will cause an
detection OFF alarm to sound, the run to pause, and
error message code 09 to be displayed.
(See Section 6.3)
OFF: All resistance changes are ignored
and the run does not stop. When running
in this mode, the symbol is displayed
on the run
screen as a reminder that this safety
feature has been turned off.
Note: Completion of certain
electrophoresis applications using cells,
such as the DCode™ and Rotofor®, may
require RRCd to be turned off.
3.3.3 Nld: No Load Detection
NLd ON ON: A drop in the current below a set
No Load Detection (Default) threshold will cause the run to pause,
OFF an alarm to sound and the error code 01
to be displayed (see Section 6.3). For
example, if an electrophoresis cell is not
connected or the current is too low (less
than 0.02mA for Manual or Basic
Methods and 0.005mA for IEF methods).
OFF: All load changes are ignored and
the run does not stop. When running in
this mode, the symbol is displayed
on the Run
screen as a reminder that this safety
feature has been turned off. Some IEF
applications may run at a
current lower than 0.005 mA, therefore,
the No Load detection may need to be
disabled in order to complete the run.
IRDA Port ON ON: Enables IR port communication.
Infrared (IR) port (Default)
communication OFF OFF: Disables IR port communication to
prevent communication interference with
other nearby IR devices, e.g., another
PowerPac power supply
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PowerPac HV – Instruction Manual
KEY CHIRP ON Activates the audible signal for keystroke.
(Default)
OFF
IRDA ON
CONTRAST HI
* KEY CHIRP ON
CHANGE EXIT RESET
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PowerPac HV – Instruction Manual
Section 4
Operation
The PowerPac HV has four operation modes:
2. Basic Methods: Nine (9) methods with up to nine steps can be stored. (see Section 4.2)
3. IEF Methods: Nine (9) methods with up to nine (9) steps can be stored. This mode
allows direct programming for high-voltage, low-current applications; also allows voltage
ramping and run delay. (see Section 4.3)
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PowerPac HV – Instruction Manual
4.1 Manual Mode
Manual mode allows the user to program and start a single-step method with a minimum
number of keystrokes. This single-step method is not stored in permanent memory.
This section describes how to program and perform a run in Manual mode.
SELECT MODE
METHODS
MANUAL IEF BASIC
EDIT CONSTANT
100 V 500 mA
400 W
TIME:UNTIMED____________
CONSTV CONSTA CONSTW
EDIT
EDIT LIMITS
100 V 500 mA
400W
TIME: UNTIMED _______
CONSTV LIMITA LIMITW
EDIT
200 V 500 mA
500W
TIME: UNTIMED____________
HRS VHRS UNTIMED
RUN
100 V 52 mA
5.2 W
00:00/00:30 00:OO HRS____
PAUSE
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PowerPac HV – Instruction Manual
4.1.1 Create, Edit and Run in Manual Mode
Step Procedure Description
1. Set up the power Refer to Section 3 for instrument setup.
supply
2. Select the mode From the Home screen, press the MANUAL soft
key to perform a single-step run in Manual mode.
SELECT MODE
When in doubt, press EDIT to toggle between
screens without losing entered values.
METHODS
MANUAL IEF BASIC
3. Select a constant Use the soft keys to select which parameter will be
parameter constant and the alphanumeric keypad to enter its
value.
EDIT CONSTANT
500 mA CONSTV: Operates with constant voltage.
400 W
TIME: UNTIMED CONSTA: Operates with constant current.
CONSTV CONSTA CONSTW
CONSTW: Operates with constant power.
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PowerPac HV – Instruction Manual
Step Procedure Description
6. Start the run Press to start a run. A run may be started from
any
screen of the EDIT loop as long as a valid
constant parameter value has been entered.
7. Monitor the run The run screen displays the elapsed time versus the
programmed time (timed runs), elapsed time
(untimed run), voltage, current and power values.
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PowerPac HV – Instruction Manual
4.2 Basic Methods Mode
Basic Methods Mode is used to create, run and store basic methods. It has the same
programming options (edit loop) as the Manual mode. The method list stores up to nine
methods, with up to nine steps each. These methods may also be transferred to or from a
PDA, through the PowerPac IR port as described in Section 5.
SELECT MODE
METHODS
MANUAL IEF BASIC
METHOD LIST
* SDS PAGE
IEF
…EMPTY 3…
____________________________
OPEN DELETE NEW
EDIT EDIT
200 V 500 mA
400 W
TIME: UNTIMED___________
CONSTV CONST CONSTW
EDIT
200 V 500 mA
400W
TIME: UNTIMED _______
CONSTV LIMITA LIMITW
Enter
EDIT Limits
EDIT
200 V 2500 mA
500W
TIME: UNTIMED _______
HRS VHRS UNTIMED
RUN: S2/4
216 V 235 mA
50 W
00:02/00:30 01:32HRS
PAUSE
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PowerPac HV – Instruction Manual
4.2.2 Create, Edit and Run a Basic Method
This section describes how to create, open, modify and run Basic Methods.
Step Procedure Description
1. Set up the power supply Refer to Section 3 for instrument setup.
METHODS
MANUAL IEF BASIC
4. Name the Method Press the up arrow key to select the method
name field.
METHOD: NAME
Use the CE button to clear the name field and the
* S1 (EMPTY)
alphanumeric keypad to enter a method name.
Refer to Section 2 for help using the keypad.
METHODS Press OK to accept the new name and return to
the METHOD SCREEN with new or edited run
name.
EDIT NAME: UNTITLED
S1 (EMPTY) Press CANCEL to abort naming the method and
return to the METHOD SCREEN.
CANCEL OK
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PowerPac HV – Instruction Manual
Step Procedure Description
5. Method steps Press EDIT to enter the edit loop.
New method • Press the up and down arrows to select an
METHOD: NAME existing step or select the (EMPTY) step to add a
new step
* S1 (EMPTY)
METHOD: NAME
Existing method
* S1
S2
100V 00:30 HRS
(EMPTY)
6. Set the constant Use the soft keys to select the constant parameter
parameter for the and the keypad to enter its value. Note that the
current step non-constant values are listed on the right side of
the screen and the time mode at the bottom.
The S#/# indicates which step is being modified. In
this case, S1/1 means editing step 1 of 1.
CONSTV: Operates with constant voltage.
CONSTA: Operates with constant current.
CONSTW: Operates with constant power.
Press EDIT to continue.
7. Enter limits for the Use the soft keys to select a parameter and the
non-constant alphanumerical keypad to enter its value.
parameters for the
current step The soft keys CONST_ or LIMIT_ change according
to the constant parameter selected in the EDIT
CONSTANT screen.
S1/1 EDIT LIMITS
500 mA Note: The constant parameter value can be
400 W
TIME: UNTIMED
changed at this point. Press its soft key and enter a
CONSTV LIMITA LIMITW new value.
LIMITV Change the voltage limit (or constant)
(CONSTV): value.
LIMITA Change the current limit (or constant)
(CONSTA): value.
LIMITW Change the power limit (or constant)
(CONSTW): value.
Press EDIT to continue.
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PowerPac HV – Instruction Manual
Step Procedure Description
8. Select the time mode Use the soft keys to select a time mode and the
alphanumeric keypad to enter the run time.
S1/1 EDIT TIME HRS: Run time is in units of hours.
500 mA
400 W VHOURS: Run time is in units of volt-hours.
TIME: UNTIMED
UNTIMED: Run is continuous.
HRS VHOURS UNTIMED
Note: Steps programmed after an untimed step are
ignored.
Press EDIT to return to the Method Screen.
9. Add a new step or To program additional steps press the down arrow
save the method key to select the empty step and repeat Steps 5–8.
If the method will not be saved, go directly to Step 10.
Press SAVE and then go to Step 10.
Press DELETE to delete a selected step.
Press the METHOD soft key to return to the Method
List.
11. Monitor the run The RUN screen is used to monitor run parameters
or to pause a run. The current step, Volts, mA and
Watts values, elapsed time versus the programmed
time, and total elapsed time are displayed on the
RUN screen. The method step is also indicated.
This run is executing Step 1 of 2.
The run may be paused by pressing the PAUSE
soft key or the key.
To edit the run parameters during PAUSE see
Section 4.5. Edits made during the run may be
saved, if the SAVE soft key is pressed at the end of
the run. Otherwise the method will remain as
originally created.
To terminate the run, press the key.
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PowerPac HV – Instruction Manual
4.3 IEF Methods Mode
The IEF Method mode is used to create, run and store Isoelectric focusing (IEF) methods.
The method list stores up to nine methods, with up to nine steps each. Special programming
options such as voltage ramping and run delay in the first step, allow better control during
IEF separations. These methods may also be transferred to or from a PDA, through the
PowerPac IR port as described in Section 5.
SELECT MODE
METHODS
MANUAL IEF BASIC
EDIT EDIT
2000 V V-SLOPE
LINEAR
TIME: 0:00 HRS
V-MAX V-SLOPE (DELAY)
EDIT
2000 V 0.00 mA
0.1 W
TIME: 0:00 HRS
V-MAX LIMITA LIMITW
EDIT
2000 V 0.10 mA
EDIT 0.1 W
TIME: 00:30 HRS___________
HRS VHRS
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PowerPac HV – Instruction Manual
4.3.1 Create, Edit, and Run an IEF Method
This section describes how to create and edit methods.
Step Procedure Description
1. Set up the power supply Refer to Section 3 for instrument setup.
2. Select the operation At the Home screen, press the IEF METHODS soft
mode key to display the IEF Method List screen.
SELECT MODE
METHODS
MANUAL IEF BASIC
METHOD: pH-3-10
S1 500V 00:05 HRS
S2 5000V 05:00 HRS
*S3 500V 00:30 HRS
METHODS DELETE
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PowerPac HV – Instruction Manual
Step Procedure Description
4. Method name Name a method.
• Press the up arrow key to select the method
name field.
• Use the CE button to clear the name field and the
alphanumeric keypad to enter a method name.
Refer to Section 2 for help using the keypad.
• Press OK to accept the new name and return to
the METHOD SCREEN with new or edited run
name.
Press CANCEL to abort naming the method and
return to the METHOD SCREEN.
6. Set the Voltage profile • Use the V-MAX soft key to enter the maximum
voltage value with the keypad for the current step
S1/1 VOLT PROFILE • Use the V-SLOPE soft key to select the voltage
V-SLOPE ramping (slow, linear or rapid).
RAPID
TIME: 01:00 HRS Press EDIT to proceed to step 8, Edit Limits.
V-MAX V-SLOPE DELAY
Use the DELAY soft key, available only in the
first step of a method, to program a run delay. No
voltage is applied during the duration of the step.
7. Set time delay • Enter the time delay and press OK to accept, and
go to the method steps screen (step 5) to edit
S1/1 EDIT TIME DELAY additional steps.
• CANCEL to return to the Volt profile screen.
DELAY: 00:00 HRS
CANCEL OK
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PowerPac HV – Instruction Manual
Step Procedure Description
8. Enter parameter limits Current or power limits must be set according to
application instructions to prevent overheating of the
IEF device .
Use the soft keys to select a limit parameter and the
keypad to enter its value. Once the limit mA or W
has been entered, the complementary value will be
automatically calculated by pressing the LIMIT soft
key or EDIT key.
V-MAX: Change the voltage limit value.
LIMITA: Set the current limit value.
LIMITW: Set the power limit value.
Press EDIT to continue.
9. Select the time mode Use the soft keys to select a time mode and the
keypad to enter the run time.
HRS: Run time is in units of hours.
VHOURS: Run time is in units of volt-hours.
Press EDIT to continue.
10. Add a new step or save To program additional steps press the down arrow
the method key to select the empty step and repeat steps 5–9.
If the method will not be saved, start the run. Press
SAVE and then start the run.
Press DELETE to delete a selected step.
Press the METHOD softkey to return to the Method
List.
12. Monitor the run The run screen is used to monitor run parameters
or to pause a run. The current step, voltage ramping,
mA and W, elapsed time versus the programmed
time, and total elapsed time are displayed on the
run screen. The method step is also indicated. This
run is executing Step 1 of 2.
The run may be paused by pressing the PAUSE
soft key or the key.
To edit the run parameters during PAUSE, see
Section 4.5. Edits made during the run can be
saved by pressing the SAVE soft key at the end of
the run.
To terminate the run press the key.
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PowerPac HV – Instruction Manual
Step Procedure Description
13. The Run Completed or Run Terminated screen is
displayed at the end of a run. Press RERUN to
repeat the run or EXIT to display the method step
list.
Connect Temperature
Probe to rear of
instrument
TEMPERATURE MODE
50 °C
______________________
EDIT
EDIT PARAMETERS
55 W 5000 V
500 mA
TIME: UNTIMED 50°C _
LIMITV LIMITA CONSTW
RUN
EDIT 216 V 235 mA
50 W
00:02/00:30 01:32HRS_____
EDIT TIME
PAUSE
55 W 5000V
500 mA
TIME: UNTIMED 50°C _
HRS VHRS UNTIMED
EDIT
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PowerPac HV – Instruction Manual
4.4.1 Create, Edit and Run in Temperature Method
Step Procedure Description
1. Set up the power supply Refer to Section 3 for instrument setup.
3. Attach probe to the Use the suction cup to attach the temperature probe
electrophoresis cell to the electrophoresis cell. Make sure the suction
cup remains attached during the run.
5. Set temperature limit Use the alphanumeric keypad to enter the desired
temperature (30°–90°C).
SET TEMPERATURE LIMIT Press the EDIT soft key to continue.
EDIT
6. Enter parameters Use the soft keys to select a parameter and the
keypad to enter its value. To change the constant
value, press its soft key and enter a new value.
EDIT PARAMETERS
5000 V LIMITV: Change the voltage limit value.
500 mA
TIME: UNTIMED 50¡C LIMITA: Change the current limit value.
LIMITV LIMITA CONSTW CONSTW: Enter the power constant value.
Note: The constant power value entered will never
be exceeded even if the cell fails to reach the set
temperature.
Press EDIT to continue.
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PowerPac HV – Instruction Manual
Step Procedure Description
7. Select the time mode Use the soft keys to select a time mode and the
alphanumeric keypad to enter the run time.
EDIT TIME
HRS: Run time is in units of hours.
55 W 5000 V
500 mA VHOURS: Run time is in units of volt-hours.
TIME: UNTIMED 50¡C
HRS VHOURS UNTIMED UNTIMED: Run is continuous.
9. Monitor the run The RUN screen is used to monitor run parameters
or to pause a run. The current step, V, voltage
ramping, mA and W, elapsed time versus the
programmed time, and total elapsed time are
displayed on the run screen.
The run may be paused by pressing the PAUSE
soft key or the key.
To edit the run parameters during PAUSE see step 6.
To Edit preference see Section 3. Edits made
during the run may be saved if SAVE soft key is
pressed at the end of the run. Otherwise the method
will remain as originally created.
To terminate the run, press the key.
Note: If the temperature reaches 5° over the set
temperature, an alarm will sound intermittently until
the situation is resolved. That is, until the power is
reduced or the temperature of the gel drops. The
alarm sounds similar to the end of run, but repeats.
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PowerPac HV – Instruction Manual
4.5 Edit a Paused Run
The PowerPac HV permits editing the run parameters during a pause in all operation
methods modes: Manual, Basic, IEF Methods, and Temperature Mode. The edit capability
during Pause is limited to the current and subsequent steps, and follows the same edit
sequence used when programming the original run. If the current step started as untimed it
cannot be changed to hours or volt-hours.
RUN: S2/4
216 V 235 mA
50 W RUN PAUSED AT:
00:02/00:30 :32HRS____
02/18/03 16:11:09
PAUSE
216V 235mA 50W
Time: 00:01 Vh: 00011___
EDIT CONTINUE
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PowerPac HV – Instruction Manual
4.5.1 Editing a Paused Run
Step Procedure Description
1. Pause the run Press the PAUSE soft key or to pause a run
that is in progress. The S#/#
indicates this run is
performing Step 1 of 2 steps.
2. Edit the method The pause window displays the current run
parameters as well as the date and time that the run
RUN PAUSED AT:
was paused.
05/27/03 12:29:00
100V 53mA 5W Press the EDIT soft key or front panel EDIT button
Time: 00:00 Vh: 00000 to edit the run parameters. Follow the instructions in
CONTINUE EDIT the appropriate section.
• Section 4.1 for Manual run editing
• Section 4.2 for Basic Methods editing
• Section 4.3 for IEF Methods editing
• Section 4.4 for Temperature mode run editing
Use the CONTINUE soft key or to resume the
run.
3. Exit the Run Completed After the run is finished, press EXIT to return to the
screen method screen to save the edits (step 4).
Press RERUN to restart the run, keeping the run
parameters the same as the last edit.
4. Save the changes From the Method screen, press SAVE to save any
changes made during the run.
Note: Pressing SAVE will overwrite the original
method.
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PowerPac HV – Instruction Manual
Section 5
Data Transfer Using the Infrared (IR) Port and Data Transfer
Software
5.1 Overview
The PowerPac HV is capable of transmitting methods and data through an infrared (IR)
port located on the front of the power supply (Figure 13). This optional PowerPac Data
Transfer Software (DTS)(catalog #164-5067) includes the PowerPac Remote application
for a PDA.
1 2 abc 3 def
EDIT 0 CE
FRONT PANEL
OUTPUT JACKS
IR port
Methods can be transmitted to and from the PDA. Run data can be transmitted to a personal
computer (PC), either directly from the power supply or through a PDA.
Using the PDA with PowerPac Remote, methods can be created, stored and beamed to
and from the PowerPac HV. The PDA may also be used as the intermediary to store and
transfer data from PowerPac to the PC (Figure 14).
The PC data transfer software function will receive, store and process run data. The PC
data transfer software will not transfer data back to the PDA or PowerPac HV.
PowerPac
Power Supply
Methods
Data
Data
PC PDA
PALM
Data
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PowerPac HV – Instruction Manual
5.2 System Requirements
Minimum PC specifications
• Windows 2000 or Windows XP Operating System
• 400 MHz processor
• 256 MB RAM minimum, 512 MB recommended
• 1,024 x 768 screen resolution, with true-color mode (24 or 32 bits)
• 10 GB hard drive
• CD-ROM drive
• Infrared (IR) port or adaptor
Minimum PDA specifications
• Palm Operating System, Version 4.0
• 8 MB memory
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PowerPac HV – Instruction Manual
Section 6
Maintenance and Troubleshooting
6.1 Maintenance
The PowerPac HV requires little maintenance to ensure reliable operation. To clean the
case, first unplug the power supply. Use a soft, damp cloth to wipe the outer case.
Keep the PowerPac HV away from liquids. Prevent them from entering the case.
Inspect the power cord regularly. Replace it if the cord is damaged or the prongs are bent
or corroded (catalog #165-9005).
Replace the temperature probe if the cord is damaged or the connectors are bent or
corroded (catalog #165-5058).
NOTCHES
Note: If the fuses need to be replaced regularly, there may be a hardware failure. Contact
Bio-Rad Technical Support.
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PowerPac HV – Instruction Manual
6.1.2 Replacement Parts
Catalog
Number Description
900-8933 Replacement Fuse, 8 A, 250 V, 5 x 20 mm, type T
165-9005 Power Cable
165-5058 Temperature Probe
6.2 Troubleshooting
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PowerPac HV – Instruction Manual
6.3 Error Messages
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PowerPac HV – Instruction Manual
Error Message Possible Cause Solution
ERROR STOP: CODE 09 1. Loose output 1. Verify all electrical
RAPID CHANGE IN R connections leading connections.
CORRECT AND CONTINUE to intermittent
RUN PAUSED connection to the
loads.
2. Cells added or 2. Pause power supply prior
removed during to adding or removing
the run. electrophoresis cells
3. Change in buffer 3. Verify buffer levels are
levels appropriate.
32
PowerPac HV – Instruction Manual
Error Message Possible Cause Solution
ERROR STOP: CODE 18 The set temperature cannot
TEMP REG ERROR be reached because:
CORRECT AND CONTINUE 1. The temperature probe is 1. Make sure the temperature
RUN PAUSED no longer connected to probe is securely fastened to
the electrophoresis cell the electrophoresis cell.
or the temperature probe
made poor contact with
the electrophoresis cell.
2. The programmed power is 2. Adjust the power so it is
insufficient to reach the set sufficient to reach the set
temperature. temperature.
Press CONTINUE after the
appropriate corrections are made.
Press CANCEL to terminate the
run
ERROR CODES: 14, 15, 17, Hardware Turn power supply off and on then
19–99 malfunction restart run. If problem persists,
contact Bio-Rad Technical
Support.
33
PowerPac HV – Instruction Manual
Appendix A
Technical Specifications
Output (programmable)
Voltage 20–5,000 V
Current 0.05–500 mA
Power (maximum) 1–400 W
Output regulation Constant voltage, constant current, constant
(with automatic crossover) power, or constant temperature
Timer control 1 min to 99 hr, 59 min
Volt-hour control Yes, 99,999 V-hr
Pause/resume function Yes
Display functions 128 x 64 pixel, yellow-green backlit graphics
LCD
Programming Up tp 9 methods, each with up to 9 steps each
Real-time editing Yes
Real-time clock Yes
Automatic recovery after power failure Yes, user-selectable; setup values maintained
Data transfer/archiving Yes
Temperature control Yes, via temperature probe; 30–90°C ± 2°C
Microampere readout and control Yes
Safety features No-load detection, sudden load change
detection, ground leak detection,
overload/short circuit protection, overvoltage
protection
Operating temperature 0–40°C
Operating humidity 0–95%
Stackability Yes
Output jacks 4 sets in parallel
Regulatory EN 61010, CE
IQ/OQ protocols Yes
Input power (actual) 90–120 or 198–264 VAC, 50 or 60 Hz,
autoswitching
Dimensions (W x D x H) 27.5 x 34 x 10 cm
34
Appendix B
Warranty and Ordering Information
Warranty
The PowerPac HV power supply is covered by a standard Bio-Rad Laboratories warranty.
Contact your local Bio-Rad representative for details of the warranty. If any defects should
occur during this warranty period, Bio-Rad Laboratories will replace the defective parts
without charge. However, the following defects are specifically excluded:
• Defects caused by improper operation.
• Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized
agent.
• Use with cables or connectors not specified by Bio-Rad Laboratories for this power supply.
• Deliberate or accidental misuse.
• Damage caused by disaster.
For inquiry or request for repair service, contact your local Bio-Rad office.
Warranty Information
Model:
Serial Number:
Date of Delivery:
Warranty Period:
Ordering Information
Catalog
Number Description
164-5056 PowerPac HV Power Supply, 100–120/220–240 V, includes power cord
164-5059 PowerPac HV Power Supply with Temperature probe, 100–120/220–240 V,
includes power cord
164-5097 PowerPac Data Transfer Software for PC and PDA
165-5098 PowerPac HV IQ/OQ Protocol Binder and Test Box
165-5099 PowerPac HV IQ/OQ Protocol Binder
35
Bio-Rad
Laboratories, Inc.
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