IVD
Achromobacter xylosoxidans Acinetobacter lwoffii Acinetobacter radioresistens Acinetobacter ursingii Ac
netobacter baumannii complex Acinetobacter baumannii Acinetobacter calcoaceticus Acinetobacter noso
mialis Acinetobacter pittii Actinomyces oris Aeromonas sp Aeromonas allosaccharophila Aeromonas cav
Aeromonas culicicola Aeromonas hydrophila Aeromonas ichthiosmia Aeromonas veronii Aeromonas sob
Alcaligenes faecalis Bacteroides_ovatus group Burkholderia gladioli Burkholderia multivorans Burkholder
cepacia complex Burkholderia ambifaria Burkholderia anthina Burkholderia cenocepacia Burkholderia cep
Burkholderia diffusa Burkholderia dolosa Burkholderia lata Burkholderia latens Burkholderia metallica Bur
deria pyrrocinia Burkholderia seminalis Burkholderia stabilis Burkholderia vietnamiensis Candida dublinie
Candida glabrata Citrobacter amalonaticus complex Citrobacter amalonaticus Citrobacter farmeri Citrobac
freundii complex Citrobacter braakii Citrobacter freundii Citrobacter gillenii Citrobacter murliniae Citrobac
werkmanii Citrobacter youngae Citrobacter rodentium Citrobacter sedlakii Citrobacter koseri Corynebacte
bovis Corynebacterium amycolatum Delftia_acidovorans group Eikenella corrodens Enterobacter aerogen
terobacter cloacae complex Enterobacter asburiae Enterobacter cancerogenus Enterobacter cloacae Enter
ter hormaechei Enterobacter kobei Enterobacter ludwigii Enterococcus gallinarum Escherichia coli Finego
magna Geotrichum capitatum Haemophilus haemolyticus Haemophilus influenzae Haemophilus parainflu
zae Hafnia alvei Klebsiella pneumoniae Klebsiella_oxytoca / Raoultella_ornithinolytica Kingella kingae Koc
kristinae Moraxella_sg_Branhamella catarrhalis Moraxella_sg_Moraxella osloensis Morganella morganii P
toea agglomerans Parabacteroides distasonis Parabacteroides distasonis Pasteurella multocida Pediococ
pentosaceus Propionibacterium acnes Proteus mirabilis Proteus vulgaris group Proteus hauseri Proteus p
Proteus vulgaris Providencia rettgeri Providencia stuartii Pseudomonas aeruginosa Pseudomonas fluores
group Pseudomonas oryzihabitans Pseudomonas azotoformans Pseudomonas brenneri Pseudomonas ce
Pseudomonas congelans Pseudomonas corrugata Pseudomonas extremorientalis Pseudomonas fluoresc
Pseudomonas gessardii Pseudomonas libanensis Pseudomonas mandelii Pseudomonas marginalis Pseud
nas migulae Pseudomonas mucidolens Pseudomonas orientalis Pseudomonas poae Pseudomonas rhode
Pseudomonas synxantha Pseudomonas tolaasii Pseudomonas trivialis Pseudomonas veronii Pseudomona
putida group Pseudomonas fulva Pseudomonas monteilii Pseudomonas mosselii Pseudomonas plecoglos
Pseudomonas putida Rothia dentocariosa Rothia mucilaginosa Salmonella sp Salmonella enterica ssp ari
Salmonella enterica ssp bongori Salmonella enterica ssp diarizonae Salmonella enterica ssp enterica Salm
lla enterica ssp houtenae Salmonella enterica ssp indica Salmonella enterica ssp salamae Serratia rubidae
rratia liquefaciens Serratia marcescens Stenotrophomonas maltophilia Streptococcus agalactiae Streptoc
anginosus Streptococcus constellatus Streptococcus dysgalactiae Streptococcus gallolyticus Streptococc
gordonii Trichosporon asahii Vibrio parahaemolyticus Vibrio vulnificus Yersinia enterocolitica Yersinia pse
MBT STAR®-Carba
IVD Assay
Rapid carbapenemase detection
MALDI-TOF
Innovation with Integrity
MALDI Biotyper
Enabling rapid detection of carbapenemase activity
The raise of multi-resistant bacteria is an
ever increasing health-care threat demanding
reliable, rapid and cost-efficient assays for
detection of resistances. Amongst the different
resistance mechanisms used by bacteria, an
important one is the enzymatic inactivation of
antibiotics by carbapenemases.
MALDI-TOF beyond microorganism identification
The MBT STAR-Carba IVD Kit
in combination with the MBT
STAR-BL IVD Module allows
rapid identification of bacteria
and detection of carbapenemase
activity in one workflow. This is the
first mass spectrometric resistance
assay expanding the application of
MALDI-TOF mass spectrometry
in clinical microbiology beyond
microorganism identification.
The MBT STAR-Carba Assay principle
The MBT STAR-Carba IVD Kit uses a This fully automated monitoring is performed
benchmark carbapenem antibiotic. Incubation by the MBT STAR-BL IVD Module – an addition
of carbapenemase producing bacteria with to the regular MBT Compass IVD software.
the antibiotic reagent results in cleavage Based on acquired mass spectra of the
(hydrolysation) of the antibiotic's ß-lactam ring, incubated solution, an automatic intensity calcu-
hence converting the antibiotic into an inactive lation of the antibiotic peaks and corresponding
metabolite. Incubation time is only 30 minutes ratio hydrolyzed / non-hydrolyzed is performed.
for Enterobacteriaceae and Pseudomonas spp. The resulting report is easy to interpret by
and 60 min for Acinetobacter spp. means of color codes, as shown in Figure 2.
After incubation, cleavage of the benchmark
antibiotic is monitored by detection of a specific
mass shift in the MALDI-TOF mass spectrum
(Figure 1).
R O R O R O
H H H H H
HN S HN S HN S
CH3 carbapenemase
CH3 - CO2
CH3
N CH3 O HN CH3 HN CH3
+ 18 Da - 44 Da
O H OH H H
OH OH OH
O O O
Figure 1: Hydrolysis of an antibiotic’s β-lactam ring leads to mass shifts that can easily be detected by MALDI-TOF
mass spectrometry
Figure 2 : Result report allowing easy and objective interpretation of the detection of carbapenemase activity
1
carbapenemase activity detected; 2 no carbapenemase activity detected
What is the MBT STAR-Carba Assay
good for?
The MBT STAR-Carba Assay is designed ...
... for rapid detection of prevalent Class A, B or D carbapenemase activity in Gram-negative
Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp.
And can be used for ...
• microorganisms derived from subculture plates
• microorganisms isolated as a bacterial pellet from positive blood culture, using the rapid
MBT Sepsityper IVD Kit workflow
• screening and confirmation purposes
And allows to ...
... rapidly identify bacteria and their carbapenemase activity on one platform in one workflow
Identification of microorganisms and
detection of their carbapenemase
activity on one system
2
3
bacteria suspen-
1 species ID
before / in parallel
sion in antibiotic
solution
with assay*
culture plate 30 min incubation
or positive
blood culture
(60 min for
Acinetobacter spp.) 4
9
result report to
physician / ward
centrifugation /
supernatant
target preparation
5
MBT STAR-BL
using supernatant
IVD Module
8 data analysis
MALDI-TOF MS
spectra acquisition
6
7
* The detection workflow is always coupled with identification of the respective microorganism.
This can be done after an initial identification run by selecting respective samples for subsequent
carbapenemase activity testing, or by parallel identification and carbapenemase activity testing.
Order Information
Pre-requisite for the MBT STAR-Carba IVD Assay is the MBT Compass IVD
(Part-No. 1832771).
Part-No. 1848467
MBT STAR-Carba IVD Kit
The MBT STAR-Carba IVD Kit provides all necessary reagents and components to
conduct the carbapenemase assay.
Part-No. 1850907
MBT STAR-BL IVD Module
Software for the analysis of co-incubation assays for the detection of carbapenemase
activity in bacteria. Incl. 2 licenses to install MBT STAR-BL IVD Module on the
acquisition system and on one additional client PC.
Part-No. 1834338
MBT Sepsityper IVD Kit
For the isolation of microorganisms from positive blood culture. Please refer to the
specific product information.
Quality control strains:
K. pneumoniae ATCC® BAA-1705™ (positive control) and K. pneumoniae
ATCC® 700603™ (negative control) are recommended for the performance of
the MBT STAR-Carba IVD Assay.
MALDI Biotyper ®, Sepsityper ® and MBT STAR® are registered trademarks of the Bruker group of companies.
Please contact your local representative for availability in your country.
Not for sale in the USA.
As of May 2021, Bruker Daltonik GmbH is now Bruker Daltonics GmbH & Co. KG.
Bruker Daltonics GmbH & Co. KG
© Bruker 05-2021, 1887705
Bremen · Germany
Phone +49 (0) 421-2205-0
[email protected] - www.bruker.com/microbiology
