Chemistry
Chemistry
SCHOOL INDORE
Internal’s Signature
Principal’s Signature
External’s Signature
School Stamp
Acknowledgement
I would like to state that this project
is my original work and I would like
to thank all those people who have
heartedly extended their cooperation
and support for making it possible to
complete this project on time.
2 INTRODUCTION
3 USES OF CAFFEINE
4 EFFECTS OF CAFFEINE
5 THEORY
6 MATERIALS REQUIRED
7 PROCEDURE
8 OBSERVATION TABLE
9 RESULTS
10 PRECAUTIONS
11 CONCLUSION
12 BIBLIOGRAPHY
AIM
To determine the amount of
Caffeine present in tea
samples
INTRODUCTION
Tea is the most commonly and widely
used soft beverage in the household. It acts as a
stimulant for CNS and skeletal muscles. That is
why tea removes fatigue, tiredness, and
headache. It also increases the capacity of
thinking. It is also used for lowering the body
temperature.
The principal constituent of Tea, which
is responsible for all these properties, is the
alkaloidcaffeine. The amount of caffeine in tea
leaves varies from sample to sample.
Caffeine constitute about 3% of the
tea’s dry weight. Tea also contain small amount
of theobromine and theophylline, which are
stimulants and xanthines similar to caffeine
Studies have found that the caffeine
contents of 1 kg black tea ranged from 22-28 mg
while in green tea ranges from 11 – 22 mg
reflecting a significant difference between the
two.
USES OF CAFFEINE
Tea samples
Red Yellow Green
Label Label Label
Weight of china 46.60 46.60 46.60
dish gms gms gms
Weight of china
47.20 47.15 47.05
dish with
gms gms gms
precipitate
2. Test
tubes and glass apparatus should be
handled with care.
3. Care
must be taken which heating the
samples.
5. There
should not be any error in the
apparatus.
CONCLUSION
The content of caffeine varies
depending on tea type, which is directly
attributed to their processing and leaf
maturity. White tea, made from the
youngest tea leaves contained the highest
caffeine content, and mate and roasted
mate tea, the lowest. The
spectrophotometric micro method proved
to be the best alternative for the
determination of caffeine content,
exhibiting the most similar results to the
HPLC analysis. According to the obtained
results, all studied teas exhibited high
antioxidant capacity, as opposed to
caffeine, indicating that the contribution of
caffeine to the antioxidant properties of
these beverages is irrelevant
BIBLIOGRAPHY
This project is made by using the
analytical data provided by the
following reference books and
websites:
https://
www.caffeineinformer.com/
caffeinecontent/tea-brewed
http://www.mayoclinic.org/
healthylifestyle/nutrition-and-
healthy-eating/indepth/caffeine/
art-20049372?pg=2
http://www.theteaspot.com/
about-tea.html