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Selective and Differential Media Lab Report

The lab report details the examination of various media plates for bacterial growth from wound, fecal, nostril, and blood samples. The results indicate the presence of coliform bacteria in both wound and fecal samples, Staphylococcus aureus in the nostril sample, and beta hemolysis in the blood agar. The report also explains the selective and differential properties of MSA and EMB media, highlighting the role of specific chemical ingredients in suppressing certain bacteria and indicating fermentation through color changes.

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15 views

Selective and Differential Media Lab Report

The lab report details the examination of various media plates for bacterial growth from wound, fecal, nostril, and blood samples. The results indicate the presence of coliform bacteria in both wound and fecal samples, Staphylococcus aureus in the nostril sample, and beta hemolysis in the blood agar. The report also explains the selective and differential properties of MSA and EMB media, highlighting the role of specific chemical ingredients in suppressing certain bacteria and indicating fermentation through color changes.

Uploaded by

Ang
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Selective and Differential Media Lab Report

Name: Angela Acosta

Examine your plates and refer to the appropriate sections of the Photographic Atlas as you
complete
this report.
Wound Sample MSA PLATE: Are isolated colonies larger than pinpoints? If so, what is the color
of the agar surrounding these colonies?

On the MSA plate, the isolated colonies are larger than the pinpoints. I formed yellow-colored
colonies, and the agar is red.

EMB PLATE: Are isolated colonies larger than pinpoints?


If so, are these colonies the same color as the agar, or are they a distinctly different color?

No growth. The agar is still red.

CONCLUSION: bacteria in your wound sample:


(Circle appropriate bacteria): Staphylococcus aureus non-aureus Staphylococcus Coliforms
Pseudomonas

It seems to be coliform.

Fecal Sample
EMB PLATE: Are isolated colonies larger than pinpoints? If so, are these colonies the same
color as the agar, or are they a distinctly different color?

The EMB plates are pinpoints. The colonies are metallic green colonies.

HE PLATE: Are isolated colonies larger than pinpoints? If so, what color are the colonies? If the
colonies are blue-green, do they have black centers?

The HE plate turns green to orange. They are larger than pinpoints and do not have black centers.

CONCLUSION: bacteria in your fecal sample


(circle appropriate bacteria): Coliforms Salmonella Shigella

This is coliform bacteria

Nostril Sample
MSA PLATE Are isolated colonies larger than pinpoints? If so, what is the color of the agar
surrounding
these colonies?

The isolated colonies are pinpoints. The agar is yellow.

CONCLUSION: bacteria in your nostril:


(Circle appropriate bacteria): Staph aureus non-aureus Staph no Staph at all

Staph aureus

Blood Agar SAMPLE USED:


Is the agar discolored around any bacterial colonies? Is the agar clear around any bacterial
colonies? Is the agar unchanged around any bacterial colonies?

The agar is discolored around the bacterial colonies. The agar is clear.

CONCLUSION—circle type(s) of hemolysis shown by your bacteria:


(α) Alpha (β) Beta gamma/none (γ)— a nostril sample may have different bacteria, and multiple
types of hemolysis

Beta hemolysis is shown.

Answer these questions:


1. What chemical ingredient makes MSA selective?
The chemical ingredient makes MSA selective because it is made of Mannitol, phenol red, and
sodium chloride. Salt makes the environment suitable for certain bacteria.
2. This chemical ingredient makes MSA selective because:
Selectivity happens when the media suppresses the growth of certain bacteria to have other
bacteria grow better.
3. What chemical ingredients make MSA differential?
The chemical ingredient that makes MSA differential is mannitol and how it can make the
bacteria ferment or change the pH of the media. This can change the color of the media.
4. How (in your own words) do those chemical ingredients work to make this medium
differential?
Certain microbes can ferment due to the sugar in the media. The phenol red allows physical
changes to the media to indicate the change in pH.
5. Pick either of these media: (EMB or HE) and describe (again, in your own words) how the
chemical ingredients in that medium work to make it both “selective” as well as “differential”.
EMB medias are both selective and differential and it is used to isolate gram-negative microbes.
EMB can also help microorganisms to ferment. Methane-blue shows when the media is in
anaerobic state.
6. How do the erythrocytes in SBA make that medium “differential”? Explain also why SBA is
not considered a “selective” medium contemplated.

On SBA media, it is not considered selective because many different organisms can grow. Three
different types of hemolysis can grow. This helps the microbiologist identify the organism better.

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