Food and Enviromental Testing
iQ-Check™ Real-Time PCR Solution
Real-Time PCR Methods for the Detection of Food and Environmental Pathogens
Visit bio-rad.com/FoodScience for more information.
The iQ-Check Solution
Providing Peace of Mind for Food Safety Testing
F
Q
Based on PCR gene amplification and real-time detection,
iQ-Check Kits use DNA primers and fluorescent probes specific to
the target organism (Figure 1). Detection of a fluorescent signal for the Q
amplified target sequences indicates the presence of the pathogen F
in the sample being tested. An internal positive control is included to
Fig. 1. Illustration of the Bio-Rad Laboratories, Inc.
validate any negative results in each well (PCR inhibitor monitoring). fluorescent probe.
Instruments and Software
iQ-Check Prep System
■ Liquid handling platform for DNA extraction and PCR plate setup
iQ-Check Prep
™
■ Completely integrated solution for pathogen detection with iQ-Check Kits
■ ull sample traceability and bidirectional laboratory information management
F
system communication
!
CFX Opus Deepwell Real-Time PCR System
1 5 10 15 20 25 30
■ Delivers sensitive 5-color multiplex performance
■ Flexible connectivity for data management and instrument control
■ Open platform
CFX Manager Software, Industrial Diagnostic Edition (IDE)
CFX Opu
s Dee
Real-T
pwell
ime PCR
System ■ Easy-to-use sample setup
■ Secure and customizable reports
■ Ability to use open software for other laboratory tests
Validations
RESEARCH
INSTITUTE
NF Validation by
iQ-Check Method AFNOR Certification AOAC NordVal Health Canada MicroVal
Aspergillus •
Campylobacter spp. •
Cronobacter spp. •
Enterobacteriaceae •
Escherichia coli O157:H7 • •
Listeria monocytogenes • • • •
Listeria spp. • • •
Salmonella Enteritidis •
Salmonella spp. • OMA* • •
Salmonella Typhimurium •
STEC 7 major O-groups •
STEC virulence genes • •
Vibrio •
STEC, Shiga toxin–producing E. coli.
* Official Method of Analysis First Action, 2017.06.
2 | BIO-RAD LABORATORIES, INC.
Easy-to-Use Protocols
Manual Solution: Four-Step Protocol Automated Solution: iQ-Check Prep System Workflow
1. Enrich sample. 1. Load enriched samples
on an iQ-Check Deep Well
Microplate or in tubes
according to the
sample worklist.
2. Extract DNA: 2. Load consumables, iQ-Check Prep
™
collect enriched sample reagents, and samples
and lyse cells. and run the iQ-Check
Prep System.
1
!
5 10 15 20 25 30
3. Set up PCR run: 3. Run real-time PCR
add extracted DNA 1
2 3 4 5 6
7 8 9 10 11 12
and get your results.
to PCR wells.
A
B
C well
DeepSystem
CFX Opus e PCR
D
Real-Tim
E
F
G
H
4. Run real-time PCR
and get your results.
Perform up to 480 tests in a single 8 hr shift
using one iQ-Check Prep and one
well
DeepSystem
CFX Opus
Real-Tim
e PCR
real-time PCR system.
Applications
iQ-Check methods can be used as a powerful alternative to standard reference methods for the
detection of food and environmental pathogens.
■ afely remove free DNA with our nontoxic, easy-to-use
S ■ armonize enrichment for Salmonella and STEC detection in
H
iQ-Check Free DNA Removal Solution raw meat
■ Use PIF Supplement to harmonize enrichment for ■ Detect threshold levels of Salmonella in poultry and rinse samples
Enterobacteriaceae, Cronobacter, and Salmonella in milk ■ Use the iQ-Check Purification Reagent treatment for difficult
powder, infant formula, cereal, and raw flour matrices, such as spices, cocoa, or cannabis products
■ Screen for Listeria spp., Salmonella, or other targets as part of ■ ake advantage of the open software and system (genetically
T
your environmental monitoring program or HACCP plan modified organism, allergen, or norovirus detection)
For a complete list of applications or to discuss how the iQ-Check System can be
integrated into your lab, contact your local Bio-Rad food safety specialist.
BIO-RAD LABORATORIES, INC. | 3
Ordering Information
Catalog # Description Catalog # Description
iQ-Check Kits Chromogenic Media for Confirmation
12010806 iQ-Check Aspergillus Kit, 96 reactions 3564748 RAPID'E.coli O157:H7 Dehydrated Base, 100 g
3578135 iQ-Check Campylobacter Kit, 96 reactions 3564744 RAPID'Listeria spp. Dehydrated Base, 500 g
3578137 iQ-Check Cronobacter Kit, 96 reactions 3554012 RAPID'Enterobacteriaceae Agar, ready to use, 200 ml x 6 bottles
3578114 iQ-Check E.coli O157:H7 Kit, 96 reactions 3563694 RAPID'L.mono Agar, 90 mm x 20 plates
12003068 iQ-Check Enterobacteriaceae Kit, 96 reactions 3563971 RAPID'Sakazakii Agar, 90 mm x 20 plates
3578124 iQ-Check Listeria monocytogenes II Kit, 96 reactions 3563963 RAPID'Salmonella Agar, 90 mm x 120 plates
3578113 iQ-Check Listeria spp. Kit, 96 reactions 3563695 AL (Agar Listeria according to Ottaviani and Agosti),
3578123 iQ-Check Salmonella II Kit, 96 reactions 90 mm x 20 plates
3578142 iQ-Check S. Enteritidis Kit, 96 reactions Instruments and Software
12013174 iQ-Check STEC SerO II Kit, 32 reactions 17007991 CFX Opus Deepwell Real-Time PCR System, 96 wells
3578139 iQ-Check STEC VirX Kit, 96 reactions 3600037 CFX96 Touch Deep Well Real-Time PCR System, 96 wells
12004306 iQ-Check S. Typhimurium Kit, 96 reactions 3594911 iQ-Check Prep System
12006574 iQ-Check Vibrio Kit, 96 reactions 3593893 CFX Manager Software, Industrial Diagnostic Edition (IDE)
iQ-Design Assays Consumables
12015337 iQ-Design Assay Aspergillus flavus, 100 reactions HSP9655XTU Hard-Shell™ 96-Well Skirted PCR Plates, pkg of 50, low profile,
12015260 iQ-Design Assay Aspergillus fumigatus, 100 reactions thin walled, white shell, white wells
12015336 iQ-Design Assay Aspergillus niger, 100 reactions HSP9955XTU Hard-Shell 96-Well Skirted PCR Plates, pkg of 50, barcoded,
12018492 iQ-Design Assay Aspergillus terreus, 100 reactions low profile, thin walled, white shell, white wells
12013770 iQ-Design Assay E. coli O104, 200 reactions 3594900 iQ-Check Deep Well Microplates, 96-well plates, 50
12015330 iQ-Design Assay Aspergillus Identification Positive Control MLL9651XTU Multiplate 96-Well PCR Plates, low profile, unskirted, pkg of 25
12018493 iQ-Design Assay Aspergillus + terreus Positive Control TLS0851XTU 0.2 ml 8-Tube PCR Strips without Caps, pkg of 120, low profile,
Supplemental Reagents thin walled, white
3594970 iQ-Check Free DNA Removal Solution, 1 vial lyophilized TCS0803XTU Optical Flat 8-Cap Strips, pkg of 120, for 0.2 ml tubes and plates,
iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial ultraclear
of iQ-Check 10x Activation Buffer (9 ml) 2240110XTU Conical Tubes, pkg of 500, 1.5 ml, with installed O-ring
3578138 iQ-Check Lysis II Reagent screwcaps, sterilized
3578136 iQ-Check Lysis Beads 3593977 X-Pierce Films, pkg of 100
12012383 iQ-Check Purification Reagent, 1 vial to make 25 ml for 96 tests
12013322 PIF Supplement, 2 g Visit our website for more information and to see how our products
Media for Sample Enrichment can be incorporated into food testing workflows:
3564684 Buffered Peptone Water Plus, dehydrated, 500 g
3554179 Buffered Peptone Water Plus, 225 ml x 6 bottles iQ-Check Real-Time PCR Kits — bio-rad.com/iQCheck
3555795 Buffered Peptone Water Plus, 3 L x 4 bags RAPID'Chromogenic Media — bio-rad.com/RapidMedia
3555790 Buffered Peptone Water Plus, 5 L x 2 bags
12013259 Buffered Peptone Water Standard, dehydrated, 500 g BIO-RAD and HARD-SHELL are trademarks of Bio-Rad Laboratories, Inc. in certain
12013258 Buffered Peptone Water Standard, dehydrated, 5 kg jurisdictions. IQ-CHECK is a trademark of Bio-Rad Europe GmbH in certain jurisdictions.
12013260 Buffered Peptone Water Standard, 5 L x 2 bags All trademarks used herein are the property of their respective owner.
3555703 Listeria Special Broth (LSB), 225 ml x 6 bottles © 2023 Bio-Rad Laboratories, Inc.
3564703 Listeria Special Broth (LSB), dehydrated, 500 g
3564753 Listeria Special Broth (LSB), dehydrated, 5 kg
12017463 Listeria Special Broth II (LSB II), 225 ml x 6 bottles
12017388 Listeria Special Broth II (LSB II), dehydrated, 500 g
12017378 Listeria Special Broth II (LSB II), dehydrated, 5 kg
12007405 Vibrio Enrichment Broth, dehydrated, 500 g
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Laboratories, Inc.
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