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Demisu Shimelis Experimental Design and Research Methodology Assignment

The document presents an experimental design assignment focused on comparing four media for bacterial growth using ANOVA. The results indicate significant differences in colony growth, with medium M3 showing the highest mean. It is recommended to use medium M3 for optimal bacterial growth based on the analysis conducted.

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21 views5 pages

Demisu Shimelis Experimental Design and Research Methodology Assignment

The document presents an experimental design assignment focused on comparing four media for bacterial growth using ANOVA. The results indicate significant differences in colony growth, with medium M3 showing the highest mean. It is recommended to use medium M3 for optimal bacterial growth based on the analysis conducted.

Uploaded by

Gadisa
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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TECHNICAL AND VOCATIONAL TRAINING INSTITUTE

Agro-Food Processing
Dairy processing Technology Department (MSc.)
Experimental Design and Research Methodology
Individual Assignment
Demisu shimelis ID NO. TTRM/135/15

Submission date 21 12 2023 G.C

Submitted to:- Instructor:-


Chala. G (Assistant Professor)

[email protected]
Experimental design assignment

A microbiologist would like to compare four media each replicated five times, which in the
final analysis help him identify the best media for bacterial growth

The experiment was conducted in the laboratory (homogeneous condition). The data on
numbers of colony growth was collected and summarized as in following table

Replication

Medium I II III IV V

M1 12 15 16 20 13

M2 10 9 11 15 10

M3 15 18 17 18 14

M4 9 8 7 8 6

Based data given above calculate the following

1. Calculate ANOVA and summarize in the form of table

2. Standard errors of treatment means

3. Standard error of differences SE(d)

4. Critical difference Least significant difference (LSD)

5. Rank the mean based on calculated (LSD)

6. CV(%)

7. Finally give brief conclusion and reasonable recommendation

1
Answer

Grand total (GT) = sum of all observations for a particular observations

GT = 76 + 55 + 82+38 = 251

Correction factor (C.F.) = (GT)2 ∕ rt = where, r = number of replication and t = number of


treatment

Correction factor (C.F.) = 2512 /5*4

=3150.05

Total sum of squares (Total SS):

(Total SS) = SS of each observation – C.F.

Total SS = ∑∑Xij 2 –C.F.

Total SS = (12)2 + (15)2 + … + (6)2 – 3150.05

= 322.95

Sum squares of treatment (SS t)

(SSt) = ∑(Ti2)/r – C.F

(SSt) = 762 + 55 2 + 822+ 382 – 3150.05

=243.75

Sum squares of error (SS E)

(SSE) = Total SS - SSt

= 322.95 - 243.35

= 79.2

2
Mean square of treatment,

MSt = SSt/(t-1)

= 243.75/3 = 81.25

Mean square of error,

MSE = SSE/t(r-1)

=79.2/16= 4.95

F-calculated for treatment,

F-cal = MSt/MSE

= 81.25/4.95 = 16.41

Table: - Analysis of variance for CRD with four treatments and of five repeats

SUMMARY
Groups Count Sum Average Variance
M1 5 76 15.2 9.7
M2 5 55 11 5.5
M3 5 82 16.4 3.3
M4 5 38 7.6 1.3

ANOVA
Source of
Variation SS df MS F-cal P-value F crit
3.86E-
Treatment 243.75 3 81.25 16.4141414 05 3.238872
Error 79.2 16 4.95

Total 322.95 19
Standard error of mean,
MS E
SE (m) = = √4.95/5 = 0.99
ri

Standard error of differences, SE(d),

SE (d) = 2MS E = √2(4.95)/5 = 1.4


r
3
Critical difference, CD or Least significant difference (LSD)

LSD = SE (d) x t-at 0.05

= 1.4 x 1.745 = 2.44

Ranking the means based on LSD:

M3 > M1 > M2 > M4

Based on LSD value we can assign different letter for each mean

7.6 c 11b 15.2a 16.4a

Coefficient of variation (CV%)

Coefficient of variation (CV%) = MS E *100


GM
= √4.95/12.55 *100

=17.7%

Conclusion and Recommendation:

Based on the ANOVA results, there is a significant difference in bacterial growth among the
four media (F-ratio = 16.41, p < 0.05). The media M3 has the highest mean number of
colony growth, followed by M1, M2, and M4. The LSD value of 2.44 indicates that a
difference in means must be at least this large to be considered significant at a 95%
confidence level. Therefore, it is recommended to use medium M3 for bacterial growth as it
showed the highest mean number of colony growth and had a significant difference
compared to other media.

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