KENDRIYA VIDYALAYA SANGATHAN, LUCKNOW REGION

Pre-Board Examination

Biotechnology (045)

Class XII (2024-25)

Max.Marks:70 Time allowed: 3 hours

General Instructions:

i) All questions are compulsory.

ii) The question paper has five sections. All questions are compulsory.

iii) Section–A contains 12 Multiple choice questions and 4 Assertion-Reasoning based questions
of 1 mark each; Section–B has 5 short answer questions of 2 marks each; Section –C has 7 short
answer questions of 3 marks each; Section-D has two case-based question of 4 marks; Section-E
has three long answer questions of 5 marks each.

iv)There is no overall choice. However, internal choices have been provided in some questions. A
student has to attempt only one of the alternatives in such questions.

Section A (MCQ)

1. The natural source of enzyme barnase and barstar, a system used to

Achieve male sterile plant is

(A) Bacillus subtilis

(B) Bartonella henselae

(C) Bacillus amyloliquefaciens

(D) Barnesville coli.

2. Artificial seeds are produced by encapsulating the somatic embryos at the

___________ Stage in a protective coating. 1

(A) Torpedo

(B) Globular

(C) Cotyledon

(D) Triangular

3. Body builders prefer to drink buffalo milk to build muscle mass. Determine the reason for this?

(a) Easier to digest

(b) Lower fat content

(c) Higher calcium and phosphorus content

(d) Balanced calorie source

4. Being a researcher, you want to improve the deficiency of certain amino acids in cereals and
legumes. Choose the technique out of the following which will be the best to achieve your goal:

(a) Plant tissue culture

(b) Adding fertilizers to soil

(c) Protein engineering

(d) Vegetative Propagation

5. An important plant secondary metabolite produced through tissue culture which has found use
in antifertility drugs is _________________

(a) Berberine

(b) Quinine

(c) Shikonin

(d) Diosgenin

6. An example of secondary metabolites produced by microbial cells include

(A) Vitamins

(B) Alcohol

(C) Acids

(D) Antibiotics

7. The peptide hormones and growth factors to promote healthy growth of animal cells in vitro
are often derived from

(A) Phenol red

(B) Antibiotics

(C) Blood serum

(D) Amino acids

8. Embryonic stem cells derived from blastocyst stage of the embryo are

___________ In nature.

(A) Totipotent (B) Pluripotent

(C) Multipotent (D) Bipotent -

9. ___________ cultures can be maintained for a prolonged period of time by repeated sub-culturing.

(A) Ovary

(B) Protoplast

(C) Callus

(D) Mass cell

10. Transgenic plants which over-express compounds like sugars, mannitol and proline

(a) Are pest resistant

(b) Are abiotic stress tolerant

(c) Produce more secondary metabolites as drugs and other important metabolites

(d) Are weedicide tolerant?

11. Which of the following statement is not correct about DNA sequencing by Sanger Method?

(a) Primers can only be extended using the single strand DNA as template.

(b) Four ddNTPs ddATP, ddUTP, ddCTP and ddGTP are needed.

(c) DNA polymerase enzyme is needed.

(d) ddNTPs are added to terminate chain (DNA) extending.

12. Alcaligenes eutrophus is associated with the production of

(a) Streptomycin

(b) Penicillin

(c) Hepatitis antigen

(d) Poly 3-hydroxybutyrate

Question No. 13 to 16 consist of two statements – Assertion (A) and Reason (R)

Answer these questions selecting the appropriate option given below:

A. Both Assertion and Reason are true and the reason is the correct explanation of the assertion

B. Both Assertion and Reason are true but the reason is not the correct explanation of the
assertion

C. Assertion is true but Reason is false

D. Both Assertion and Reason are false

13. Assertion: Foaming is a problem in most microbiological processes.

Reason- It is caused due to the presence of fatty acids and silicones in the culture medium.

14 Assertion: Protoplasts are plant cells without cell wall.

Reason (R): Protoplasts can be utilized for producing somatic hybrids.

15.Assertion: The regulation of pH is essential for survival of mammalian cells.

Reason (R): Animal cell cultures mostly make use of Bicarbonate carbon dioxide buffering system
to maintain pH.

16. Assertion: During plant tissue culture, the explants are treated with sodium hypochlorite.

Reason (R): Sodium hypochlorite helps in sterilization of the regenerated plants.

Section B (2 marks)

17. What information can be obtained from the following Database retrieval tools?

(a) Locus link

(b) Taxonomy browser

18. Name the technique that helps to study the entire protein profile from a given cell type. Briefly
explain the principle of this method.

19. Differentiate between Primary cell cultures and Secondary cell cultures?

20. For genetic transformation vector, which two DNA sequences are cloned in the T-DNA region
of Ti-plasmid in place of unwanted DNA sequences?

OR Define BV, PER

21. How does the metagenomics approach help in identifying novel genes? Explain the process.

How will you perform laboratory screening for micro-organism producing an antibiotic?

Section C (3 marks)

22. Write a note (Any three)

a) Erythropoietin
b) OKT3
c) Herceptin
d) Interleukin 2
23. How does the charge relay system operate in chymotrypsin?

Or

(i) An oncologist is performing colony formation assay on tumour cells from a patient. What is
he trying to determine?

(ii) Animal cells growing in culture show the property of contact inhibition. Relate this to what
happens in an adult human body.

24. A. Why has sickle cell trait been selected in population where malaria is endemic?

B. What is the molecular basis of sickle cell anaemia?

C. How can we differentiate between a normal RBC and Sickle cell RBC?

Or

What is BLAST? Write the principles that underlie BLAST search.

25. Illustrate essential steps involved in raising a chimeric mouse using stem cell technology.

26. In animal cell culture, osmolarity of the culture medium has significant role in cell growth and
function. Justify. Which ingredients decides osmolality of the medium?

27. SNPs can be used to understand genomics variation in response to medicine Explain.

OR How can CML patients can be identified by the FISH technique?

28. Transgenic crops are facing challenges globally. What are the major concerns about these
GM crops?
Section D

29. Carefully read the below mentioned flow-chart and answer the questions that follow

Flow-chart scheme for isolation of Protein A is as given below:

(a) Write whether Protein A is of intra or extra cellular origin. 1

(b) Which step in the given purification scheme is metabolite specific? 1
(c) Give the purification scheme for isolation of Humulin from E. coli 2
OR
(c) Why is it advisable to use lesser number of steps for downstream Processing?

30. Animal cell culture

Animal cells can be grown in glass or plastic vessels with nutrient medium. These need to be
given fresh medium periodically. The infant animal cells grow only to adulthood and not any
further. While growing, they show phenomenon of contact inhibition, but

(i) What is contact inhibition? 1

(ii) Which property of cells is studied by oncologists to determine whether cells are normal or
cancerous? 1

iii) As in vivo environment is different from in vitro environment, how does it affect cells grown
in culture? Mention any two. 2
OR

(iii) Mortality is associated with all normal animal cells. Justify giving reason.
Section E (5 marks)

31. (i) Explain the reason for therapeutic use of whey proteins. 2
(ii) Name any two diseases that have been treated with whey. 2
(iii) Curd is advised to be administered with antibiotics. Why? 1

Or

Explain the technique of Sanger’s DNA sequencing mentioning the role of ddNTPs in it.

32. (i) According to WHO estimates, approximately 228 million children are affected due to
Vitamin A deficiency. How has genetic engineering provided/developed a solution to the same?
Explain.
(ii) Why is nutritional quality of cereals and legumes limited?
(iii) Name two essential amino acids which are deficient in pulses. 3+1+1
Or

(a) Illustrate the important parts of a mass spectrometer with the help of a suitable diagram.

(b) Explain how proteins are volatilised as well as analysed by a mass spectrometer.

(c) What is the major attraction for using this technique as a characterization tool for proteins.

33. A. Write the basic steps with principles (starting from an explant) involved in obtaining a
regenerated plant. Suggest any two applications of plant tissue culture.

OR

B. Indicate how intergeneric somatic hybrids can be made. Give an example. What are cybrids?
How are edible vaccines advantageous over recombinant vaccines produced by bacterial
fermentation?