lec 5 - oven
lec 5 - oven
Hot air oven is advice is used in the laboratory for drying and sterilizing glass and
metal containers used for tests in the laboratory, surgical tools.
The temperature range covered by ovens is between 50-250 °C . Dry heat sterilization of
clean material is conducted at 180 °C for two hours in the oven. Upon being heated by high
temperature dry air, humidity is evaporated from glassware and thus the possibility of any
remaining biological activity is eliminated.
Dry heat sterilization technique requires longer exposure time (1.5 to 3 hours) and
higher temperatures than moist heat sterilization.
MECHANICAL PARTS
The coat (The external chamber) : made of aluminum or stainless steel, covered with a
protective film of electrostatic paint.
Fiber glass: used to maintain the internal temperature since it is a good insulator of heat
(prevent the access of heat from inside the device to the outside ).
The chamber: The internal chamber is made of aluminium or stainless steel material with very
good heat transference properties. It is isolated from the external chamber by insulating
material which maintains high temperature conditions internally and delays the transference of
heat to the exterior.
The shelves (mesh): The shelves are plates on which the objects are placed. made of stainless
steel grids so that air circulates freely around objects requiring drying or dry heat sterilization.
ELECTRICAL PARTS
Heat is generated through sets of electrical resistors transferring this thermal energy to
the chamber. These resistors are located in the lower part of the oven and heat is transferred
and distributed by natural or forced convection (in oven with internal ventilators).
Sterilizing by dry heat is accomplished by conduction. The heat is absorbed by the
outside surface of the item, then passes towards the Centre of the item, layer by layer. The
entire item will eventually reach the temperature required for sterilization to take place.
ADVANTAGES
1. A dry heat cabinet is easy to install and has relatively low operating costs.
2. It penetrates materials.
3. It is nontoxic and does not harm the environment.
4. it is noncorrosive for metal and sharp instruments.
DISADVANTAGES
1. Time consuming method because of slow rate of heat penetration and microbial killing.
2. High temperatures are not suitable for most materials.