Microbiology Reviewer

Serratia
Hafnia
Gram Positive
→ Catalase ←

(+) (-) Citrate (+)

“Pero Sa CHEEKS”
Staphylococcus Streptococcus
Micrococcus Providencia
Serratia
Citrobacter
CPON Hafnia
→ Catalase (+); Oxidase (-) ← Ewingella
Enterobacter
Staphyloccocus Klebsiella
All Entero except Pleisomonas Salmonella enteritidis
Francisella
Oxidizer : Stenotrephomonas, Acinetobacter
→ Catalase ←

CPOP (+) (-)

→ Catalase (+); Oxidase (+) ← “LBC CAN” “WELGA”

Microccocus Listeria Weissella conpusa

Neisseria, Haemophilus Bacillus Erysipelothrix
Pleisiomonas, Aeromonas, Vibrio Corynebacterium rhusiopathiae
Oxidizer : Burkholderia, Pseudomonas Clostridium Lactobacillus
Actinomyces Gardnerella vaginalis
Nocardia Arcanoacterium

DNASE (+)
“SSSMo”
All bacilli are Gram (-), except :
S. pyogenes “MCCBELLANGA”
S. aureus
Serratia marcescens Mycobacterium
Moraxella Corynebacterium
Clostridium
Bacillus
Erysipelothrix
Indole (+) Listeria
“May PEKPEC Po” Lactobacillus
Actinomyces
Morganella Nocardia
Proteus vulgaris Gardnerella
E. coli Arcanobacterium
Klebsiella oxytoca
Providencia
Edwardsiella tarda
Citrobacter koseri All bacilli are Gram (-), except :
Plesiomonas “MCCBELLANGA”

Mycobacterium
Corynebacterium
VP (+) Clostridium
“KEESH” Bacillus
Erysipelothrix
Klebsiella Listeria
Ewingella Lactobacillus
Enterobacter Actinomyces
Microbiology Reviewer

Nocardia Late Lactose Fermenter

Gardnerella → A or K/A ←
Arcanobacterium “Cit Ser Yer Haf Sa Shi is Late”

Citrobacter
Serratia
All cocci are Gram (+), except :
Yersinia enterocolitica
“NeVer Mind”
Hafnia
Salmonella arizona
Neisseria
Shigella sonnei
Veillonella
Moraxella

Non Lactose Fermenter

→ K/A ←
Slow Urease Producers
“Sa Shi PPM Edward Erwin”
“CKEYS”
Salmonella
Citrobacter
Shigella
Klebsiella
Proteus
Enterobacter gergoviae
Providencia
Yersinia enterocolitica
Morganella
Serratia
Edwardsiella
Erwinia

Rapid Urease Producers

“TPUNCH”
H2S (+)
T. mentagrophytes “SPACE”
Providencia, Proteus, Morganella
Salmonella
Ureaplasma
Proteus
Nocardia
- mirabilis : indole (-)
C. neoformans
- vulgaris : indole (+)
H. pylori
Arizonae
Citrobacter freundii
Edwardsiella tarda
Congenital Infection
“ToRCHES”

Toxoplasma Capsule
Rubella “Even Some Super Killers Have Pretty Nice
Cytomegalovirus Big Capsules”
Herpes
Escherichia coli
Syphilis
Streptococcus pneumoniae
Salmonella
Klebsiella pneumonia
Rapid Lactose Fermenter Haemophilus influenzae
→ A/A H2S (-) ← Pseudomonas aeruginosa
“EKE” Neisseria meningitidis
Bacteroides fragilis
Escerichia Cryptococcus neoformans
Klebsiella
Enterobacter
Bipolar bodies inclusions
“Bipolar is BAY”

B. pseudomallei
Actinomycetemcomitans
Microbiology Reviewer
Alpha : S. pneumoniae
Yersinia pestis
Beta : Group A (large) = S. pyogenes
Group B (small) = S> agalactiae
Group C = S. dystagalactiae; S. equi
SPUTUM Gamma : Group D

S. pneumoniae : rust colored K antigen

K. pneumoniae : currant jelly - Capsular
P. aeruginosa : blue - green - Heat labile and polysaccharide

O antigen
Neutral Red - Somatic
“SMAC” - Heat sable and lipopolysaccharide

SSA : fermenter (red) H antigen

MacConkey : nonfermenter (colorless) - Flagellar
- Denatured by heat / alcohol / proteins

Growth at 42C
Phenol Red
“CAP”
“MX CUT”
Campylobacter jejuni
MSA
Acinetobacter baumann
XLD
Pseudomonas aeruginosa
CTA
Urease Growth at 65C
TSI
Legionella
**fermenter (yellow)
**nonfermenter (no color change; orange to red) <10C : Cold Enrichment

Listeria monocytogenes
Yersinia enterolitica
Bromothymol Blue
“CHOT”

Citrate Growth at pH 3
Hektoen
Oxidation fermentation Lactobacillus acidophilus
TCBS
Growth at pH 8 - 10
**fermenter (yellow)
**nonfermenter (no color change; blue green) Vibrio

★ Gentamicin BAP : S. pneumoniae

★ Bacitracin CAP : H. influenzae Listahan ng mga BULL - BULL
★ Cystine Trypticase agar : Neisseria “BEECCY”
★ McCoy cells : C. trachomatis
★ TSB : Brucella B. burdorferi : bull’s eye rash
E. histolytica : bull’s eye amoeba
Enterobacter : fisheye colonies (EMB)
Novobiocin Test C. diphtheria : bull’s neck infection
→ Differentiate CONS Cushing’s syndrome : buffalo hump
(coagulase neg - staph) ← Y. enterocolitica : bull’s eye colonies (CIN)
→ (+) PYR ←

R (<16 mm) : S. saprophyticus

S (>16 mm) : S. epidermidis
Microbiology Reviewer

Wag na mag - SWIMMING N. elongata : smooth, glistening and claylike

“PMSLAV”

P. aeruginosa : swimmer’s ear

Odor and Color Appearance
M. marinum : swimming pool granuloma
Schistosoma : swimmer’s itch
Proteus : chocolate cake or burnt chocolate smell
Legionella : shower hear
B. hemolytic strep : butter odor
Aeromonas : fresh water
B. cepacia : dirt like odor
Vibrio : salt water
A. Faecalis : apple or strawberry odor
C. violaceum : ammonium cyanide (almond);
violet
★ S. pneumoniae : lancet, bullet shape,
P. multocida : musty / mushroom
capnophilic, lobar pneumonia N. animaloris : popcorn
C. difficile : horse stable / barnyard odor
★ S. agalactiae : neonatal, meningitis,
H. influenzae; Pasteurella : musty odor
endometritis in mother S. aureus : old socks
P. aeruginosa : fruity, grape - like, corn, tortilla
★ S. pyogenes : toxic shock syndrome, erysipelas
E. corrodens : bleach
★ Viridans : cavities, dental infection, SBE in Nocardia : freshly - flowered grass
S. merscencens : brick red
damage heart valves
Kluyvera spp. : blue
★ Enterococci : multi - drug resistance, P. melaninogenica : brown - black
C. diphtheriae : gum - metal black
nosocomial infection, PHV
Pits the agar : “CEK”
➔ Cardiobacterium
➔ Eukenella
Motility ➔ Kingella

T. pallidum : corkscrew
Campylobacter : darting
Inclusion Bodies
Capnocytophaga : gliding
Proteus : swarming
Negri bodies : rabies virus
L. monocytogenes : tumbling (at RM)
Virchow bodies : leprosy
E. rhusiopathiae : brush pattern
Asteroid bodies : Sporothrix schenckii
Vibrio cholerae : shooting star
Cowdry A : herpes virus
Kingella kingae : twatching
Cowdry B : polio virus
Guarneri bodies : smallpox
Torres councilman bodies : yellow fever
Colony Morphology Medlar bodies / sclerotic bodies :
chromoblastomycosis
P. aeruginosa : green metallic sheen on BAP; Semilunar bodies : P. faciparum
serrated confluent Bollinger bodies : Fowlox
E. coli : green metallic sheen on EMB Donovan bodies : K. granulomatis /
Enterobacter : fisheye on EMB Corynebacterium
Diphtheroids : dry and waxy rough eyes Clue cells : gardnerella vaginalis
Y. enterocolitica : bull’s eye on CIN Morula bodies : Ehrlichia
H. influenzae : dew drops / satellite colonies Halberstaedter prowazek : chlamydia
B. pertussis : mercury droplet on Regan Lowe; Owl’s eye : CMV
shiny half pearls on blood agar Sulfur granules : Nocardia
B. anthracis : medusa head n BAP Babes ernst : corynebacterium
Actinomyces : molar tooth Koilocyte : HPV
Mycoplasma : fried egg Aschoff bodies : rheumatic fever
MTB : cauliflower Elementary bodies : reproductive bodies of
Salmonella : snowflakes on BG agar chlamydia
Moraxella : hockey pack colonies
Nocardia : brittle, crumbly, wrinkled
S. aureus : buttery / creamy yellow
Microbiology Reviewer

Fever Mycoplasma

Pontiac fever : Legionella

Malta fever : Brucella
Herpesviridae
Glandular fever : EBV
Rat fever : Spirillum, Streptobacillus moniliformis
HHV 1 HSV 1 (cold / oral sores)
Oroya fever : Bartonella bacilliformis
Shipping fever : Pasteurella HHV 2 HSV 2 (genital warts)
Rabbit fever : Francisella
Relapsing fever : Borrelia recurrentis HHV 3 Varicella zoster (VZV)
Scarlet fever : S. pyogenes
Pretibial / Fort bragg fever : Leptospira HHV 4 Epstein barr (EBV)
autumnalis
7 day fever : Leptospira hebdomanis HHV 5 Cytomegalovirus (CMV)
Marsh fever : Leptospira grippotyphosa
Beaver fever : G. lamblia HHV 6 Exanthem subitum
Boutonneuse / Mediterranean fever : Rickettsia
conori HHV 7 Roseola infantum

HHV 8 Kaposi's sarcoma

Diarrhea

EHEC : hamburgers Bacterial Meningitis

EIEC : Diarrhea with WBCs “AIMS”
ETEC : Traveller’s diarrhea / Montezuma’s
revenge Streptococcus agalactiae
EPEC : Infantile diarrhea Haemophilus influenzae
Norwalk virus : adult gastroenteritis Neisseria meningitidis
Coronavirus : Summer gastroenteritis Streptococcus pneumoniae
Rotavirus : Infantile gastroenteritis (winter period)
Salmonella : chicken
Vibrio parahaemolyticus : summer diarrhea Viral Meningitis
“EPEC”

Enterovirus
Childhood Disease Poliovirus
Echovirus
1st 14 day measles Rubeola Coxsackievirus
2nd Scarlet fever S. pyogenes

3rd German measles rubella Quality Control Sched

4th Filatow - dukes / S. aureus Each use Gas pak jar, ONPG
SSS
Daily Gram stain, refrigerator, oxidase,
5th Erythema Parvovirus B19 catalase
infectiosum
Weekly Antibiotic disks, reagents, autoclave
6th Exanthema HHV - 6
subitum Monthly Biosafety cabinet

Semi - RPM of centrifuge

annual
Atypical Pneumonia
Annual Biosafety airflow, balance
Listeria
Francisella
Chlamydia psittaci
Microbiology Reviewer

Tests “Listahan ng mga YOSI boys”

Forshay test : Francisella tularensis S. schenkii : cigar shaped (yeast)

Dick’s test : Streptococcus pyogenes T. mentagrophytes : cigar shaped (microconidia)
(susceptibility) E. histolytica : cigar shaped (chromatoid bodies)
Schick & Eleck test : C. diphtheria M. leprae : cigar packets
Ocular test of anton : Listeria monocytogenes C. diphtheria : cigarette packet / picket fences
Francis test : Streptococcus pneumoniae
Ascoli test : Bacillus anthracis
Frei test : chlamydia
“Listahan ng mga ITIM”
String test : Vibrio, K. pneumoniae
Machadi test : T. cruzi P. falciparum : black water fever
Schultz - charlon test : S. pyogenes (diagnostic) L. donovani : black fever
Widal test : Salmonella Y. pestis : black death / plague
Weil - felix test : Rickettsia
Lepromin test : Mycobacterium leprae
Urea breath test : H. pylori
Whiff and schiff test : Gardnerella vaginalis “Listahan ng mga Ka - loka - like”
Casoni test : E, granulosus
Bachmann : T. spiralis Leishmania : mistaken for histoplasma
Moan test : A. lumbricoides Blastomyces : resembles myelin globules in
Mantoux test : M. tuberculosis sputum
C. neoformans : resembles lymphocytes in CSF.

“Hindi sweet si Jason AMOA”

→ Assachrolytic ← “Listahan ng mga Patatas ni Sarah”
No sugar (not utilize sugar)
B. pertusis : potato blood glycerol (mercury
Acinetobacter iwoffi droplets colony)
Moraxella Fungi : potato dextrose agar; potato flake agar
Oligella ➔ Potato : enhance sporulation of fungi
Alcaligenes S. odorifera : potato odor
Source of V. factor : potato extract
➔ S. aureus
➔ Neisseria
Curved ➔ S. pneumoniae
“HAC” ➔ Yeast extract
Helicobacter
Anicobacter ★ Botox : most potent toxin
Campylobacter ★ Tetranoplasmin : 2nd most potent toxin
★ Corynebacterium : 3rd most potent toxin

Biochemically inert
Causes of Meningitis by Age Group
Shigella (enterobacteriaciae)
E. corrodens (HACEK) Neonates S. agalactiae Pinaka
M. ulcerans (Mycobacterium) → buruli ulcers (0 to 1 mo) ma-AGAng stage
ng buhay

Children H. influenza Mabilis

“Listahan ng mga UBAS” (1 to 5 yrs) maimFLUENZA
han
S. aureus : cocci in grapelike clusters
T. mentagrophytes : microconidia in grapelike Adolescent N. Di tayo nagging
clusters (5 yrs to 29 meningitidis MEN
Adenovirus : viral CPE of swollen grape-like yrs)
cluster
Geriatrics S. Tumatandnag may
Microbiology Reviewer
BSL - 2
pneumoniae NYUMONYA
- Organism acquired through ingestion,
Immunoco L. imMONOcompro
mpromised monocytogene mised percutaneous, and mucous membrane
s exposure
- Example : Salmonella, HBV, enterics,

Bacteria with PILI Shigella, S. aureus, HIV

“PENS BYC”

Pseudomonas BSL - 3
E. coli - Organisms possible for aerosol transmission
Neisseria
Salmonella (inhalation)
Bordetella - Example : MTB, Rickettsia, Brucella,
Yersinia
Campylobacter Arbovirus, C. immitis

★ Lag : adjustment BSL - 4

★ Log : exponential - Organism may expose extreme risk to lab
★ Stationary : plateau workers
★ Declines : death - Organism that cause life - threatening
disease
Motility Lecithin Lipase Lactose Glucose - Example : EBOLA
ase

C. - + - + +
Categories of Potentially
perfri
ngens Infectious Agents of Bioterrorism

C. - - + - -
tetani Category A Agents

C. + - - - + - Agents that pose the greatest health threat,

botuli easily transmitted and highly infectious; high
num
mortality rate
C. + - - - + - Example : B. anthracis, F. tularensis
diffici
le
Category B Agents
Biosafety Level - Moderately easy to disseminate or transmit;
BSL - 1 moderate morbidity and low mortality.
- Organism with no known potential of - Example : Brucella
infecting healthy people
- Example : B. subtilis (lab contaminant) Category C Agents
M. gordonae (tap water bacillus) - Organisms that can be engineered for mass
dissemination in the future. High morbidity
and mortality rates
Microbiology Reviewer
- Example : Nipah, Influenza virus, EPEC
Hantavirus - Infantile diarrhea
- Nosocomial
Glucose Maltose Lactose Sucrose Fructos - Outbreak of diarrhea among hospital nurseries
e

N. + - - - -
gonor EHEC
rhoea - Severe bloody diarrhea
e
- Hemolytic uremic syndrome
N. + + - - -
meni
ngitid
is Rickettsiaceae
N. + + + - - Spotted Fever Group
lacta “CRAS”
mica
R. conorri : cat flea ricketsia
N. + + + + R. rickettsia
sicca R. akori
R. sibirica
M. - - - - -
catar
rhalis
Typhus Fever Group
“PT”
★ C. tetani : spastic paralysis
★ C. botulinum : flaccid paralysis R. prowazekii
R. typhi
★ C. diphtheriae : palatal paralysis

★ R. akari and D. tsutsugamushi : mite bites ★ Capnophiles : 5% - 10%

★ R. prowazekii : louse feces ★ Mesophiles : 35 - 37C

★ R. typhi : flea feces ★ Psychrophiles : 0 - 20C

★ R. conorii : tick bites ★ Thermophiles : 50 - 125C

★ Halophiles : 8% - 10%

ETEC
- traveler’s diarrhea
- Turista; cholera - like ★ S. aureus : food poisoning, toxic shock syndrome,

- Montesuma’s revenge characterized by scalded skin syndrome, ritter’s disease

profused watery diarrhea ★ S. epidermidis : UTIm stitch abscess, prosthetic heart

valve infection

EIEC ★ S. saprophyticus : most commonly cause of UTI,

- Bloody stool pyelonephritis, cystitis in those with indwelling

- Invades intestinal epithelium causing catheters

shigella - like infection ★ S. lugdunensis : endocarditis

Microbiology Reviewer
★ Smallest DNA virus: Parvoviridae
RNA Virus
★ Largest DNA virus: Poxviridae
Togaviridae - Double stranded DNA except:
Coronaviridae Parvoviridae (ssDNA)
Retroviridae - Icosahedral Capsid except: : Poxviridae
Picornaviridae - Replicate in Nucleus except: Poxviridae
Caliciviridae
(cytoplasm)
Flaviviridae
Reoviridae
Orthomyxoviridae
Paramyxoviridae NAKED
Rhabdoviridae “Pa Pa Aa”
Bunyaviridae
Papovaviridae
Arenoviridae
Filoviridae Parvoviridae
Adenoviridae

★ Single-stranded RNA except: Reoviridae

★ Replicate in Cytoplasm except : Retroviridae & Enveloped
Orthomyxoviridae → Labile : ether sensitive ←
★ Helical Capsid except: “Po He He”
○ Reoviridae
○ Togaviridae Poxviridae
○ Picornaviridae Herpesviridae
○ Caliciviridae Hepadnaviridae
○ Retroviridae
○ Flaviviridae National Standard Reporting Scale
(RITM/ DOH)
NAKED O No AFO seen in 300 visual fields
“PRC”
+n 1-9 AFO seen in 100 fields
Picornaviridae
Reoviridae 1+ 10-99 AFO seen in 100 fields
Caliciviridae
2+ 1-9 AFO seen per field in at least 50
fields
★ Smallest RNA virus : Picornaviridae 3+ More than 10 AFO seen per field at
★ Largest RNA virus: Paramyxoviridae least 20 fields

- Rhabdoviridae : has Helical Symmetry but

★ Autoclave : 121 deg for 15 mins at 15 psi
is shaped like a bullet.
★ Infectious waste : 132 deg C for 30 mins at 15
psi
DNA Virus ★ Boiling water : 100 deg C for 15 mins
“HHAPPPy” ★ Pasteurization : 63 deg C for 30 mins

Herpesviridae Types of Flagella

Hepadnaviridae ● Peritrichous : Flagella on all sides
Adenoviridae ● Amphitrichous : Flagella in both sides
Papovaviridae ● Lopotrichous: Tuft of flagella on one or both
Parvoviridae sides
Poxviridae ● Atrichous : Lacks flagella
● Monotrichous : has one flagella
Microbiology Reviewer

Oxidase (+)
“PVNCHAP”

P. aeruginosa
V. cholerae
Neisseria
Campylobacter
Helicobacter
Aeromonas
P. shigelloides
Moraxella

★ Streptolysin (s) : O2 stable-exposed;

Non-immunogenic
★ Streptolysin (o): O2 labile- not exposed :
Immunogenic
 Hemolytic Pattern
Common Causes of Loefler’s Syndrome
● Alpha-hemolysis : S. pneumoniae, Viridans
group (S. mutans, S. mitis) “TASHTT”
● Beta-hemolysis : S. pyogenes, S. agalactiae - Strongylous
● Gamma-hemolysis : E. faecalis, S. bovis - A. Lumbricoides
● Alpha hemolysis: occurs with prolongs - S. Stercoralis
refrigeration (24C) - Hookworm
- T. canis
- Pyoverdin : green pigment - T. cati
- Pyocyanin: blue pigment
- Pyomelanin: brown / black pigment Agents of Pelvic Inflammatory Disease (PID)
- Pyorobin : red pigment
● C. trachomatis
“MYCOBACTERIAL” ● M. hominis
● N. gonorrhea
Group 1 : Photochromogens

“MASK” Blood Bag Contaminants

- Maritum (cutaneous infection from water
exposure) ● Pseudomonas fluorescens
- Asiaticum ● Y. enterocolitica
- Simae (pulmonary disease “rare”) ● Serratia liquefaciens
- Kansasii (pulmonary disease #2 AIDS, A.K.A.
Yellow Bacillus)

Group 2: Scotochromogens GRAM STAIN

- In strep, prepare Bacterial Smear
“SSG has the X factor” - Decolorizer: most critical step.
- Scrofulaceum (cervical lymphadenitism children
- Szulgai (Pulmonary disease
- Gordonae (Lab contaminant; AKA Tap water
Reagent Purpose Gram + Gram -
Bacillus)
- Xenopi (Pulmonary disease) Crystal Violet Primary Violet Violet
- Fiavesceris (contaminant / normal flora) stain

Gram’s Iodine Mordant Violet Violet

Group 3 : Non Chromogen
- Avium (pulmonary disease, #1 AIDS) 95% Alcohol / Decolorizer Violet Colorless
- Intracellulare ( Non pathogenic, AKA Battery Acetone
Bacillus)
Safranin Counterstai Violet/purp Red
n le
Group 4: Rapid Growers

“Fast Craft Sa Pier”

- Fortuitium (pulmonary disease) Agents of Otitis Media
- Chelonae (pulmonary disease) - M.catarrhalis
- Smegmatis - S. pneumoniae
- Prilei - H. influenzae
 Vp tt Pink to red

Urease test Red

Lysine Ornithine Arginine Moellers Purple
E. cloacae - + + LIA Purpe
E. aerogenes + + - Deaminase Phenylalanine : green,
Tryptophan: brown
E.coli + + -
Citrate Blue
Cirtobacter - + -
Malonate utilization Blue
K. pneumoniae + - -

Pseudomonas + - -

B. cepacia + - -

S. maltophilia + - -
Agent of NonGonococcal Urethritis
- C. trachomatis
- M. hominis
- D. urealyticum

All about Aids

● AIDS (overall): C. albicans

● AIDS (tuberculosis): M. ovium, intracellular
complex
● AIDS Pneumonia: P. jirovecii
● AIDS meningitis: C. neoformans
DIFFERENT TESTS ● AIDS dementia: T. gondii
● Alps diarrhea : C. parvum
Niacin Yellow
MILK BACTERIOLOGY
Nitrate Reduction Initial: REd , with Zinc :
No color change
● BLUE MILK: Pseudomonas syncyanea
Iron Uptake test Rusty brown ● YELLOW MILK: Flavobacterium synxatum
● BLUE GREEN MILK: Pseudomonas aeruginosa
Heat stable catalase Vigorous bubbling
● RED MILK: Serratia Marcescens
Arylsulfatase test Pink ● SOURING OF MILK: Streptococcus lactis
● SLIMY/ ROPY MILK: Alcaligenes viscolactis
Tween 80 hydrolysis Pink ● PROTEOLYTIC ACTION ON COAGULATED
MILK: B. subtilis Haybacillus
Tellurite Reduction Black

ONPG Yellow
CYSTIC FIBROSIS Px.
Indole Red ring
"Most to Least Pathogen"
MR test Red
 ● CVA: Campylobacter spp.
- P. AERUGINOSA ● CIN: Yersinia an Aeromonass upp.
- B. CEPACIA ● CAP: Haemophilus spp & Neisseria
- C. AUREUS ● CMG: Clostridium spp.
● TWEEN 80: Chlamydospore of Candida
Albicans
POSITIVE COLOR RESULTS. ● CTBA: Corynebacterium Diphtheriae
● CTA: Neisseria
RED "IMVUN" ● DEOXYCHOLATE AGAR: Enterobacteriaceae
● DEOXYCHOLATE CITRATE: Salmonella
- Indole test Shigella
- Methyl red ● DIEUDONNE'S MEDIA: Vibrior
- Voger Proskauer
- Urease
- Nitrite Reduction
STREPTOLYSIN O
YELLOW “TOT - Labile
- TSI - Antigenic
- ONPG - Sub-surface
- TCBS - Incubated anaerobically

BLUE, GREEN" CAMA"

- Citrate STREPTOLYSIN S
- Acetate
- Maunate - Stable
- Acetamide - Non-antigenic
- Surface
CULTURE MEDIA - Incubated Aerobically

● AMIE’S MEDIA: Transport Media; Maintain

Viability
● ALKALINE PEPTONE WATER: Vibrio SELECTIVE MEDIA::FOR ISOLATION
● BBE: Bacteroides
● BEA: Group D Streptococci and Enterococci - THAYER MARTIN: VCN
● BEA-V: cultivation of Vancomycin resistant - MODIFIED THAYER MARTIN: VCN-T
Enterococci - MARTIN LEWIS VCA-T
● BHI: High protein Media - NEW YORK CITY: VCA-T
● BSA: Salmonella typhi: Black colonies - GC-LECT: VCA-TL
● BA: Cultivation of Fastidious Microorganism
● BCDA: Francivella Tularensis
● BGM: Bordetella Pertussis NOTE:
● BGA: Salmonella other than S. typhi
● BCTE-A Legionella upp. - Vancomycin : prevent gram positive.
● CSA: Staphylococcus - Colistin : prevents gram negative.
● CBA: Campylobacter upp. - Nystatin : prevent Fungi | anti-Fungal
● CTB: Recovery of Campylobacter spp. - Anisomycin : anti-fungal
● CBM: Transport Media - Amphotericin B: prevent fungi
● CM: Nocardia Streptomyces - Trimetoprim: prevent swarming organism
 - Lincomycin further inhibit gram positive ● S. marcescens
organism ●

ORGANISM WITH HALO

AGENT OF PELVIC INFLAMMATORY DISEASE. "SMSACS"
(PID) ● S. aureus
● Moraxella
"CMN" ● S. marcescens
- C. trachomatis ● A. hydrophila
- M. hominis ● C. minituima
- N. gonorrhea ● S. pyogenes
●
AGENT OF ONS MEDIA:
PAD: PPMC
"HMS"
- H. influenzae ● Proteus
- M.catarrhalis ● Providencia
- S. pneumoniae ● Morganella
● Cronobacter sakazakii
AGENTS OF BACTERIAL VAGINOSIS:

"MMPPP"
- Mobilindus LACTOSE + : SEEKECC
- M. hominis
- Prevotella ● S. odorifera
- Peptostreptococcus ● E.coli
- Porphyromonas ● Ewingella
● Klebsiella
● Enterobacter
UREASE: PPM KEYC ● C. sakazakii
● Citrobacter
● Proteus
● P. rettgeri LYSINE DECARBOXTLASE + :
● Morganella
● Klebsiella “SHEEPSEK”
● E. cloacae - Salmonella
● Yersinia - Hafnia
- Escherichia
NON MOTILE: SKY - E. aerogenes
● Shigella - Plesiomonas
● Klebsiella - Serratia
● Yersinia (except Y. enterocolitica) - Edwardsiella
- Klebsiella
GELATINAVE + : 4°C PPSS

● P. mirabilis
● P. vulgaris ARGININE DECARBOXYLASE + :
● S. odorifera
“SPECC” DIFFICULT TO DIFFERENTIATE:
- S. enteritidis
- Plesiomonas 1. Taenia
- E. cloacae 2. Hymenolepis eggs
- C. Jakozakii 3. Opisthorchis vs Clonorchis
- Citrobacter 4. Clonorchis us Heterophages Vs Metagonimus.
5. Ascarid (A. lumbri, T. cati, T.'cans)
ORNITHINE DECARBOXYLASE + : 6. P. westermani Vs D.latum
7. Fasciola vs Fasciolopsis
“SHEEP SYMPECC” 8. Hookworm & strongy
- S. sonnei
- Hafnia
- E.coli MTB COMPLEX
- Edwardsiella
Nitrate Niacin TCH
- Plemonas
- S. marcescens M. TB + + +
- Yersinia
- Morganella M. Bovis - - -
- P. mirabilis M. Africanum V V V
- Enterobacter
- Citrobacter
- Cronobacter

SHIGELLA SEROTYPES:

- A. dysenteriae
- Flexneri
- Buydii
- Sonnei

TOP 5 INFECTIONS IN THE WORLD:

● E. coli
● S. aureus
● Enterococcus
● Coag Neg staph
● P. aeruginosa

HEXACANTH

● TAENIA (Best)
● ECHINOCOCCUS
● HYMENOLEPIS