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BRCA1

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Lưu An An
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10 views2 pages

BRCA1

Uploaded by

Lưu An An
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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15:57 22/06/2024 Primer3 Output (primer3_results.cgi release 0.4.

0)

Primer3 Output

No mispriming library specified


Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 529 20 59.94 50.00 6.00 3.00 GGACAGGTCCCTTGTTTCAA
RIGHT PRIMER 702 20 59.98 55.00 2.00 1.00 CTGGTCTTTCCTTGCCTCTG
SEQUENCE SIZE: 840
INCLUDED REGION SIZE: 840

PRODUCT SIZE: 174, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 TTTGAAACTTGCCCACTCCATTTGAGTGGAAGCATGGCCTGATCACCCATGGCGTGCCTT

61 TATTGGCACTTTGGTTTTGGTTTGAATTGCTTGACAGGATTGGTCTTGGGAACTTGCCTA

121 CTCCATTTGAGTGGAAGTGTGGCCTGATCACCCATGGTGTGCCTCTCCTGGCACTTTGGT

181 TTTTATTTTTGACTTGACTTGGATTGCTTGATACTTTGGTTTTGGTTTTGACCTGGCTTG

241 GATTTCTGGATACTCTGATTTTGGTTTGGTGCAAACTGCAAAACTGTGTGTGCGCCCTTT

301 TTACCGGTTCTTTGTTTTGTGGTGTGTTTGTGGTGTGAGCGTGGTGTTTTGTCTTGAAGA

361 AGCATAGGTCAGGCACGAATAAGCCCACCCCACTAGGAACTATGTTGACAAATTTCAAAA

421 AAGAATCTGAGGGAGACTATGGAGTGCTATGACACCAGGAAAACTTAAAACTTTGTGTAA

481 GATAGACTGGCCAGCATTAGAGGTAGGTTGGCCATTAGAAGGAAGCCTGGACAGGTCCCT
>>>>>>>>>>>>

541 TGTTTCAAAGGTATGGCACAAGGTAACCTATAAGCCAAGGAACCCAGACCAGTTCCCTTA
>>>>>>>>

601 CAGAGACACTTGATTAGAGCTGGTTTTAGACCCCCCACCCCCAACACATAGTGGTTGAGA

661 GAACAGCAGCATAAGTGGCTGGCAGAGGCAAGGAAAGACCAGCAGAGAGAGAGAAAGGAA
<<<<<<<<<<<<<<<<<<<<

721 AGAGACAGAGAGGAAAAGAGGAAAAGAGAGAGAGGAAGAGACAGGAAGAGACAGACAGAG

781 AGTCAAGGATAGAGAGAGAGAGAGAGAGAGAGACACACACACACACACACACAGAGAGAG

KEYS (in order of precedence):


>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 529 20 59.94 50.00 6.00 3.00 GGACAGGTCCCTTGTTTCAA


RIGHT PRIMER 707 20 60.13 55.00 2.00 2.00 CTCTGCTGGTCTTTCCTTGC
PRODUCT SIZE: 179, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

https://bioinfo.ut.ee/cgi-bin/primer3-0.4.0/primer3_results.cgi 1/2
15:57 22/06/2024 Primer3 Output (primer3_results.cgi release 0.4.0)
2 LEFT PRIMER 321 20 60.05 55.00 2.00 2.00 GGTGTGTTTGTGGTGTGAGC
RIGHT PRIMER 536 20 60.25 55.00 6.00 0.00 ACCTGTCCAGGCTTCCTTCT
PRODUCT SIZE: 216, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

3 LEFT PRIMER 488 20 60.23 55.00 6.00 0.00 TGGCCAGCATTAGAGGTAGG


RIGHT PRIMER 707 20 60.13 55.00 2.00 2.00 CTCTGCTGGTCTTTCCTTGC
PRODUCT SIZE: 220, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

4 LEFT PRIMER 304 20 59.87 45.00 4.00 0.00 CCGGTTCTTTGTTTTGTGGT


RIGHT PRIMER 536 20 60.25 55.00 6.00 0.00 ACCTGTCCAGGCTTCCTTCT
PRODUCT SIZE: 233, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 6338 0 0 0 14 0 1957 2348 0 9 44 98 1854
Right 6398 0 0 0 16 0 2351 1962 0 1 44 97 1925
Pair Stats:
considered 471, unacceptable product size 454, high end compl 3, ok 14
primer3 release 1.1.4

(primer3_results.cgi release 0.4.0)

https://bioinfo.ut.ee/cgi-bin/primer3-0.4.0/primer3_results.cgi 2/2

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