BIOLOGY CRASH

BATCH
COURSE FOR NEET

Biotechnology and its Applications

BASIC MATHS &
Strategies for Enhancement in Food
Production
By Vikas Shukla
1. What was the world’s first ever mammal to be successfully cloned
from an adult cell?
(A) Sheep (B) Monkey
(C) Cow (D) Calf
2. Humulin is the term used for
(A) A powerful antibiotic
(B) A new digestive enzyme
(C) New Research integrated to human welfare
(D) Human insulin
3. Which Vitamin has been transferred to golden rice?
(A) Vit-B12 (B) Vit-A
(C) Vit-D (D) Vit-C
4. The Ti plasmid used in genetic engineering is obtained from:

(A) Bacillus thuringiensis

(B) E. Coli
(C) Agrobacterium rhizogenes
(D) Agrobacterium tumefaciens
5. The test used to diagnose AIDS is:

(A) ELISA (B) Southern Blot

(C) ESR (D) PCR
6. Bacillus thuringiensis (Bt) strains have been used for designing
novel

(A) bio-fertilizers
(B) bio-metallurgical techniques
(C) bio-mineralization processes
(D) bio-insecticidal plants
7. Bt toxin kill the larvae of certain insects:

(A) by binding of activated toxin on mid gut epithelial cells, creating

pores, leading to swelling and lysis.
(B) by stopping transcription of larval cells.
(C) by altering central dogma taking place in the cells of gut of
larva.
(D) by stopping protein synthesis.
8. Cry protein is obtained from:

(A) Bacillus thuringiensis

(B) Bacillus subtilis
(C) Clostridium welchi
(D) E. coli
9. Cry-genes have been introduced in:

(A) cotton and corn

(B) rice
(C) potato and soyabean
(D) all of the above
10. Bt toxin is harmful to insects like:

(A) lepidopterans (tobacco budworm, armyworms)

(B) coleopterans (beetles)
(C) dipterans (flies and mosquito)
(D) all of the above
11. RNA interference (RNAi) technique has been devised to protect the
plants from nematode is silenced by ______ produced by the host
plant.

(A) dsDNA (B) ssDNA

(C) dsRNA (D) target proteins
12. Tobacco plants resistant to a nematode have been developed by the
introduction of DNA that produced (in the host cells):

(A) both sense and anti-sense RNA.

(B) a particular hormone.
(C) an antifeedant.
(D) a toxic protein.
13. C-peptide of human insulin is:

(A) a part of mature insulin molecule.

(B) responsible for formation of disulphide bridges.
(C) removed during maturation of pro-insulin to insulin.
(D) responsible for its biological activity.
14. The first human drug made using recombinant DNA technology was:

(A) glyphosate (B) tPA

(C) insulin (D) erythropoietin
15. The first clinical gene therapy was given in 1990 to a 4 years old girl
with enzyme deficiency of:

(A) adenosine deaminase (ADA)

(B) tyrosine oxidase
(C) monamine oxidase
(D) glutamate dehydrogenase
16. In some children, ADA deficiency can be cured by:

(A) bone marrow transplantation

(B) enzyme replacement therapy
(C) both (A) and (B)
(D) none of the above
17. The site of production of ADA in the body is:

(A) bone marrow (B) lymphocytes

(C) blood plasma (D) monocytes
18. Genes of interest can be selected from a genomic library by using:

(A) restriction enzymes

(B) cloning vectors
(C) DNA probes
(D) gene targets
19. DNA or RNA segment tagged with a radioactive molecule is called
___________.

(A) vector (B) probe

(C) clone (D) plasmid
20. The transgenic animals are those which have:

(A) foreign DNA in some cells.

(B) foreign DNA in all of their cells.
(C) foreign RNA in all of their cells.
(D) both (A) and (C)
21. Today, transgenic models exist for many human diseases which
includes:
(i) Cancer (ii) Cystic fibrosis
(iii) Rheumatoid arthritis (iv) Alzhiemer’s disease

(A) (i) and (iii) only

(B) (ii) and (iii) only
(C) (i), (ii) and (iii) only
(D) all of these
22. The protein -1 antitrypsin is used to treat the:

(A) cancer
(B) rheumatoid arthritis
(C) Alzheimer’s disease
(D) emphysema
23. Maximum number of existing transgenic animals is of:

(A) fish (B) mice

(C) cow (D) pig
24. GEAC stands for:

(A) Genome Engineering Action Committee

(B) Ground Environment Action Committee
(C) Genetic Engineering Approval Committee
(D) Genetic and Environment Approval Committee
25. How many varieties of rice has been estimated to be present in
India?

(A) 2,000 (B) 20,000

(C) 200,000 (D) 2,000,000
26. Which variety of rice was patented by a U.S. company even though
the highest number of varieties of this rice is found in India?

(A) Sharbati Sonara (B) Co-667

(C) Basmati (D) Lerma Roja
27. The use of bioresources by multinational companies and other
organizations without proper authorisation from the countries and
people concerned without compensatory payment is called:

(A) bioethics (B) biopiracy

(C) bioterror (D) bioweapon
28. Which step of Government of India has taken to cater requirement of
patent terms and other emergency provisions in this regard?

(A) Biopiracy act

(B) Indian patents bill
(C) RTI act
(D) Negotiable instruments act
29. Find out the incorrect statement.

(A) Human protein used to treat emphysema is -1 antitrypsin.

(B) Human insulin is being commercially produced from a
transgenic species of Agrobacterium tumefaciens.
(C) Rosie, the first transgenic cow, produced human protein
enriched milk.
(D) Cry I Ab endotoxins obtained from Bacillus thuringiensis is
effective against corn borers.
30. Which one of the following statement(s) is/are correct about Genetic
Engineering Approval committee (GEAC)?

(A) It will make decision regarding the validity of GM research.

(B) It will make the safety of introducing GM - organism for public
services.
(C) If genetic modification of organism can have unpredictable
results when such organisms are introduced into the ecosystem.
Therefore, the Indian government has set up organisation such
as GEAC.
(D) All of the above
31. Which one of the following statements is correct?

(A) The proteins encoded by the genes cry I Ac and cry II Ab control
cotton bollworms.
(B) Protein encoded by cry I Ab controls corn borer.
(C) Both (A) and (B)
(D) Proteins encoded by cry I Ac and cry I Ab control flies.
32. Which of the following is a correct statement?

(A) “Bt” in Bt-cotton indicates that it is genetically modified

organism produced through biotechnology.
(B) Somatic hybridization involves fusion of two complete plant
cells carrying desired genes.
(C) The anticoagulant hirudin is being produced from transgenic
Brassica napus seeds.
(D) “Flavr Savr” variety of tomato has enriched the production of
ethylene which improves its taste.
33. Which of the following statement(s) is/are correct?

(A) The procedure for chemical safety testing / toxicity is the same
as that used for testing toxicity of drugs.
(B) Transgenic animals are more sensitive to the toxic substances
than non-transgenic animals.
(C) Golden rice, a genetically engineered rice has high vitamin A
(retinol) content.
(D) All of the above
34. Which of the following statements is correct?

(A) The current interest in the manipulation of microbes, plants

and animal has raised serious ethical issues.
(B) One possible risk of genetic engineering is the accidental
production of dangerously resistant microorganisms.
(C) Although risks are possible, genetic engineering appears to
offer more of contribution to human welfare than threats.
(D) All of the above
35. Select the correct statement.

(A) Genetic engineering works only on animals and has not yet been
successfully used on plants.
(B) There are no risks associated with recombinant DNA
technology.
(C) The first step in PCR is heat which is used to separate both the
strands of target DNA.
(D) DNA from one organism will not bond to DNA from another
animal
36. Transgenic animals has been successfully used for producing:

(A) transgenic mice for testing safety of polio vaccine before use in
humans.
(B) transgenic models for studying new treatments for certain
cardiac diseases.
(C) transgenic cow – rosie which produces high fat milk for making
ghee.
(D) animals like bulls for farm work as they have super power.
37. Silencing of mRNA has been used in producing transgenic plants
resistant to:

(A) bollworms (B) nematodes

(C) white rusts (D) bacterial blights
38. A transgenic food crop which may help in solving the problem of
night blindness in developing countries is:

(A) golden rice (B) Bt soyabean

(C) flavr - savr tomato (D) starlink maize
39. Which of these is used as vector in gene therapy for SCID?

(A) Arbovirus (B) Rotavirus

(C) Retrovirus (D) Parvovirus
40. The genetic defect, adenosine deaminase (ADA) deficiency may be
cured permanently by:

(A) administering adenosine deaminase through injection

(B) bone marrow transplantation
(C) enzyme replacement therapy
(D) introducing isolated gene from marrow cells producing ADA
into the cells at early embryonic stages
41. Genetically engineered bacteria have been successfully used in the
commercial production of:

(A) human insulin (B) testosterone

(C) thyroxine (D) melatonin
42. Biotechnology deals with industrial scale production of
biopharmaceuticals and biological products using genetically
modified:

(A) microbes only

(B) fungi only
(C) plants and animals only
(D) all of the above
43. Bt toxin is:

(A) intracellular lipids.

(B) intracellular crystalline protein.
(C) extracellular crystalline protein.
(D) intracellular polysaccharide.
44. RNA interference involves:

(A) synthesis of mRNA from DNA.

(B) synthesis of cDNA from RNA using reverse transcriptase.
(C) silencing of specific mRNA due to complementary RNA.
(D) interference of RNA in synthesis of DNA.
45. The RNAi stands for:

(A) RNA inactivation (B) RNA initiation

(C) RNA interference (D) RNA interferon
46. What is the disadvantage of using porcine insulin (from pig) in
diabetic patients?

(A) It leads hypercalcemia.

(B) It is expensive.
(C) It may cause allergic reactions.
(D) It can lead to mutation in human genome.
47. In order for gene therapy to be most effective, genes should be
inserted in:

(A) WBC (B) RBC

(C) stem cells (D) all of these
48. Which of the following is based upon the principle of antigen-
antibody interaction?

(A) PCR (B) ELISA

(C) r-DNA technology (D) RNA
49. Match the following and choose the correct combination from the
options given below:

Column-I Column-II
A. Escherichia coli I. -lactalbumin
B. Bacillus thuringiensis II. Humulin
C. Rosie III. Bt toxin
D. Agrobacterium IV. Vector
tumefaciens

(A) A-II, B-I, C-IV, D-III (B) A-II, B-I, C-III, D-IV
(C) A-II, B-III, C-I, D-IV (D) A-IV, B-I, C-II, D-III
50. Match the items given in column – I with those in column – II and
choose the correct option.

Column-I Column-II
A. GMO I. Increased self life
B. Flavr – Savr tomato II. Bioresources
C. Biopiracy III. rDNA
D. E. coli IV. Insulin
(A) A-III, B-I, C-II, D-IV (B) A-II, B-I, C-III, D-IV
(C) A-II, B-III, C-I, D-IV (D) A-IV, B-I, C-II, D-III
51. Match column-I with column-II and identify the correct option.

Column-I Column-II
A. Gene therapy I. Rhizobium
B. Biofertilizer II. Cry gene
C. Bt cotton III. SCID
D. Humulin IV. Diabetes

(A) A-II, B-I, C-IV, D-III (B) A-III, B-I, C-II, D-IV
(C) A-II, B-III, C-I, D-IV (D) A-IV, B-I, C-II, D-III
52. India and China have more than 70% of world livestock population
and produce the following percentage of world farm.

(A) 10 % (B) 25 %
(C) 40 % (D) 50 %
53. When breeding is between animals of the same breed it is called
______________, while crosses between different breeds are called
______________.

(A) out-breeding; inbreeding

(B) inbreeding; out-breeding
(C) out breeding; cross-breeding
(D) cross-breeding; inbreeding
54. Inbreeding depression:

(A) usually increases fertility only.

(B) usually reduces productivity only.
(C) usually reduces fertility and productivity.
(D) usually increases fertility and productivity.
55. Which one of the following is a new breed of sheep developed in
Punjab by crossing Bikaneri ewes and Marino rams?

(A) Hisardale (B) White Leghorn

(C) Assel (D) Langshan
56. The 'mule' is the result of:

(A) inbreeding depression

(B) out-breeding
(C) cross-breeding
(D) inter-specific hybridization
57. MOET stands for:

(A) Multiple Ovulation Embryo Transfer Technology

(B) Multiple Ovulation Energy Transport Technology
(C) Method of Ovulation Energy Transfer Technology
(D) Method of Ovulation Energy Transport Technology
58. Which of the following stage is transferred to surrogate mothers in
livestock breeding experiments?

(A) Unfertilized eggs (B) Fertilized eggs

(C) 8 to 32 celled embryo (D) Frozen semen
59. A branch of science that deals with the maintenance of hives of
honeybees for the production of honey is called ______.

(A) aquaculture (B) pisciculture

(C) apiculture (D) sericulture
60. Which one of the following is a freshwater fish?

(A) Catla (B) Rohu

(C) Common carp (D) All of these
61. Which one of the following is/are marine fish?

(A) Rohu (B) Hilsa

(C) Mackerel (D) Both (B) and (C)
62. Which of the following statements about breeding is incorrect?

(A) By inbreeding, purelines cannot be evolved.

(B) Continued inbreeding, especially close inbreeding reduces
fertility and productivity.
(C) Cross-breeding allows desirable qualities of two different
breeds to be combined.
(D) Inbreeding exposes harmful recessive genes that are eliminated
by selection
63. Read the following statement(s) and answer the question.
(i) Animal husbandry is the industrial practice of breeding and
raising livestock.
(ii) Dairy farm management deals with processes which improve
the quality and quantity of milk production.
(iii) Poultry is the class of domesticated birds used for food or eggs.
Which of the following statement (s) is/are correct?

(A) Only (i) (B) Only (iii)

(C) Both (ii) and (iii) (D) All of these
64. Which of the following statement(s) is/are correct regarding
inbreeding?
(i) It is the mating of more closely related individuals within the
same breed for 4-6 generations.
(ii) It decreases homozygosity.
(iii) It exposes harmful recessive genes that are eliminated by
selection.
(iv) Inbreeding depression results due to continued Inbreeding,
especially close – inbreeding, may reduce fertility and productivity.

(A) (i) and (ii) (B) (iii) and (iv)

(C) (ii), (iii) and (iv) (D) (i), (iii) and (iv)
65. Which of the following includes all the characteristics described
given below?
(i) In this method of breeding, the mating of animals occurs with
same breed.
(ii) They have no common ancestors on either side of their pedigree
upto 4 - 6 generations.
(iii) It is best for those animals that are below average in
productivity in milk production, growth rate in beef cattle.
(iv) It helps to overcome inbreeding depression.

(A) Inbreeding (B) Out-breeding

(C) Out-crossing (D) Cross-breeding
66. Which of the following statement(s) is/are correct?
(i) Fishery is an industry, which deals with catching, processing
and marketing of fishes and other aquatic animals such as prawn,
crab, lobster, edible oyster, etc. that have a high economic value.
(ii) Both aquaculture and pisciculture are same.
(iii) The development and flourishing of the fishery industry is
called silver revolution.

(A) Only (i) (B) Both (ii) and (iii)

(C) Both (i) and (iii) (D) All of these
67. Which of the following statement(s) is/are correct?
(i) Out-breeding is the breeding of the unrelated animals which
may be between individuals of the same breed but having no
common ancestors for 4 - 6 generations or between different breeds
or different species.
(ii) Cross-breeding allows the desirable qualities of two different
breeds to be combined.
(iii) In interspecific hybridization, male and female animals of two
different related species are mated.

(A) Only (i) (B) Only (iii)

(C) Both (ii) and (iii) (D) All of these
68. Which of the following pair is correctly matched?

(A) Sericulture – Fish

(B) Pisciculture - Silk moth
(C) Aquaculture – mosquitoes
(D) Apiculture - Honey bee
69. Which of the following is the process of choosing parent organisms
for the characteristic that is wanted in their offspring?

(A) Active selection

(B) Reproductive selection
(C) Selective breeding
(D) None of the above
70. Hisardale is a new breed of sheep developed in Punjab by one of the
breeding techniques in which superior male of one breed is mated
with superior females of another breed. Identify the breeding
technique from the option given below.

(A) Inbreeding (B) Out crossing

(C) Out breeding (D) Cross breeding
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