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Nanoencapsulation
Technologies for the
Food and Nutraceutical
Industries
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Nanoencapsulation
Technologies for the
Food and Nutraceutical
Industries
Edited by
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or
mechanical, including photocopying, recording, or any information storage and retrieval system, without
permission in writing from the publisher. Details on how to seek permission, further information about the
Publisher’s permissions policies and our arrangements with organizations such as the Copyright Clearance
Center and the Copyright Licensing Agency, can be found at our website: www.elsevier.com/permissions.
This book and the individual contributions contained in it are protected under copyright by the Publisher
(other than as may be noted herein).
Notices
Knowledge and best practice in this field are constantly changing. As new research and experience broaden
our understanding, changes in research methods, professional practices, or medical treatment may become
necessary.
Practitioners and researchers must always rely on their own experience and knowledge in evaluating and
using any information, methods, compounds, or experiments described herein. In using such information
or methods they should be mindful of their own safety and the safety of others, including parties for whom
they have a professional responsibility.
To the fullest extent of the law, neither the Publisher nor the authors, contributors, or editors, assume any
liability for any injury and/or damage to persons or property as a matter of products liability, negligence or
otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the
material herein.
ISBN: 978-0-12-809436-5
3 Encapsulation by nanoliposomes 74
Mehmet Demirci, Muhammed Y. Caglar, Bilal Cakir, İbrahim Gülseren
3.1 Introduction 74
3.2 Design of liposomes 79
3.2.1 MFGM phospholipids 80
3.2.2 Marine lipids 81
3.2.3 Cholesterol 81
3.2.4 Phenolic compounds 82
3.3 Determination of encapsulation efficiency 83
3.4 Encapsulation of hydrophilic materials 84
3.4.1 Phenolic compounds 84
3.4.2 Bioactive peptides 89
3.5 Encapsulation of hydrophobic materials 90
3.5.1 Hydrophobic polyphenols 90
3.5.2 Bioactive lipids 90
3.5.3 Vitamins 91
3.6 Novel techniques in the design of nanoliposomes 91
3.6.1 LbL deposition 92
3.6.2 Supercritical CO2 technologies 92
3.6.3 Combinatorial methods 93
3.6.4 Drying of liposomes 93
3.6.5 Interactions with mucus 94
3.7 Phytosomes: highly efficient delivery of phytochemicals 94
3.8 Incorporation into food systems 96
3.8.1 Vitamins and minerals 96
3.8.2 Phenolic compounds 97
3.8.3 Enzymes 97
3.9 Bioactivities of nanoliposomal encapsulation systems 97
3.9.1 Antimicrobial activity 98
3.9.2 Anticarcinogenic activities 100
3.9.3 Other bioactivities demonstrated by liposomal dispersions 101
3.10 Digestion of bioactive bearing nanoliposomes 102
3.10.1 In vitro digestion 103
3.11 Conclusions and future perspectives 104
Acknowledgments 104
Contents xi
10
Nanocapsules formation by nano spray drying 346
Cordin Arpagaus, Philipp John, Andreas Collenberg, David Rütti
10.1 Introduction 346
10.2 Nano spray drying 350
10.2.1 Droplet generation 352
10.2.2 Drying of droplets 353
10.2.3 Particle collection 354
10.3 Optimizing the Nano Spray Drying Process Parameters 355
10.3.1 Influences of process parameters 355
10.3.2 Drying gas flow rate, humidity and temperature 359
10.3.3 Droplet size 361
10.3.4 Particle size 362
10.3.5 Solid concentration 365
10.3.6 Feed rate 366
10.3.7 Product yield 368
10.3.8 Organic solvent instead of water 371
10.3.9 Particle morphologies 371
10.3.10 Encapsulation efficiency and active compounds loading 375
10.3.11 Controlled release of active compounds 376
10.3.12 Stability of active compounds during nano spray drying 376
10.3.13 Storage stability 376
10.3.14 Challenges in nano spray drying 377
Contents xv
Index 591
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Front cover figure description
The prepared illustration on the book cover exhibits a nanospray drier in which
nanoparticles are achieved via spray drying by which particle sizes can be reduced
to 300 nm and yields up to 90% are possible, moreover the sample amount can be
as low as 1 mL. The bioactive compounds within the foods illustrated above this
device include: oleurupein for olive, lycopene for tomato, ascorbic acid (vitamin C)
for berries, quercetin for apple, and safranal for saffron, which is then entrapped in
the capsule. The capsule next to the device represents the process of encapsulation in
which our bioactive compound (safranal) is incorporated within the nanocapsule. The
scientific name of the flower in the first place of the capsule is Crocus sativus, com-
monly known as the “saffron crocus.” The name saffron comes from the Persian word
“za’faran”; it is this famous pistil made of three stigmas (filaments) which when dried
up gives the spice saffron. To date the undisputed center for saffron production is Iran,
where the production process dates back to over 3000 years ago. Iran manufactures
over 90% of the 250 tons produced globally annually, which has the potential to be
used in food, cosmetics, and traditional medicine areas. In the second place the chemi-
cal structure of safranal is illustrated, next the spiral structure of amylose molecule is
evident, which can be used as the host molecule to incorporate guest molecules within
it. Lastly, the 3D form of safranal is represented and included in the capsule as the
core material.
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List of contributors
Anilda Guri Canadian Research Institute for Food Safety, Guelph, ON, Canada
xxii List of contributors
Sanaz Haratifar Canadian Research Institute for Food Safety, Guelph, ON, Canada
T he Red Cross has been the Angel of Mercy to the soldier lying on
his fevered couch in hospital, for it brought to him succor and a
message of hope and cheer. But coming in contact with it at a time
when he was least able to apprehend the efforts which brought the
help so sorely needed, he is apt to regard the Red Cross as a field
institution and fails to appreciate the labor and sacrifice of the
women at home who made its work of mercy possible.
The work of the Nova Scotia Branch of the Canadian Red Cross
Society has been the raising of money, manufacturing and
distribution of goods, visiting and supplying the needs of hospital
ships, trains, military and convalescent hospitals, and arranging
concerts, drives and entertainments at private homes for returned
men. Its work did not terminate with the declaration of peace but
still goes on in almost as great a measure as in time of war, and will
continue to do so as long as one returned soldier remains in our
hospitals.
Many Nova Scotians, as well as returned men, do not appreciate
the magnitude of the task accomplished by the Nova Scotia Branch of
the Canadian Red Cross, and it is only possible here to give a brief
outline of its activities. The women of every city, town and village in
the Province gave the best of their thought, substance and action in
order that the citizen soldier of Nova Scotia, whether in the fighting
line or in hospital convalescing from wounds might have every
possible comfort. At the end of 1915 the Province had thirty-one
chartered and two hundred and sixty-eight auxiliary branches of the
Red Cross. Every village and hamlet had its workers who contributed
a steady stream of supplies and an enormous amount of labor
devolved upon the Provincial Branch at Halifax, which acted as a
Clearing House for all branches throughout the Province.
The officers of the Provincial Branch during the War, with slight
changes of office but not of personnel, were as follows:
Patrons.
President.
MRS. CHARLES
ARCHIBALD.
MRS. DENNIS.
Vice-Presidents.
Mrs. F. H. Sexton.
Mrs. F. B. McCurdy.
Mrs. Chas. Archibald.
Mrs. Hector McInnis.
Mrs. M. A. Curry (Hon.)
Hon. Secretary.
Hon. Treasurer.
H. E. Mahon, Ess.
Executive Committee.
Mrs. T. Benson.
Mrs. A. W. Jamieson.
Mrs. G. S. Campbell.
Mrs. F. Woodbury.
Mrs. W. J. Armitage.
Mrs. E. A. Kirkpatrick.
Mrs. N. Duffus.
Mrs. A. Costley.
Mrs. W. E. McLellan.
Mrs. F. B. McCurdy.
Mrs. H. W. Cunningham.
Mrs. L. J. Donaldson.
Mrs. G. A. MacIntosh.
Mrs. W. R. Foster, Dartmouth.
Mrs. A. P. Scarfe, Dartmouth.
Mrs. Crathorne, Dartmouth.
Mrs. W. S. Munnis.
Miss Jean Forrest.
Miss Constance Bell.
Mrs. Sedley Thompson.
Miss Ella Ritchie.
Mrs. McKay McLeod, Sydney.
Mrs. M. A. Curry.
Mrs. T. S. Rogers.
Mrs. Frank Hope.
Mrs. J. A. Clark.
Mrs. I. B. Schaffner.
Mrs. P. J. McManus.
Mrs. W. T. Allen.
Mrs. J. W. Longley.
Mrs. Geoffrey Morrow.
Also the President of each Chartered Branch.
Advisory Board.
Mr. J. L. Hetherington, Chairman.
Mr. J. A. Neville.
Mr. H. E. Mahon, Treasurer.
Mr. C. C. Starr.
Mr. Chas. V. Monoghan.
Mr. Emil Gaboury.
Mr. R. Corbett.
Mr. H. McF. Hall.
Mr. W. H. Dennis.
Mr. F. A. Gillis.
The annual report of the Provincial Branch for the year ended
October 31, 1916, shows a balance on hand at first of year of
$10,961.26 and receipts for the year of $52,667.62. Disbursements
amounted to $56,584.89, of which the principal items were:
Remittances to Head Office, Toronto $29,278
38
Remittances to endow 50 cots in Princess Patricia
Hospital 2,500 00
Material for surgical dressings and garments, and
wool for socks 18,163 17
Office maintenance and expenses at Shipping Pier 1,388 12
Clayton Military Convalescent Home 1,993 67
Contributions to Special Objects 1,141 21
$202,867 65
Annapolis $9,713 54
Antigonish 5,598 33
Cape Breton 42,150 29
Colchester 20,444 29
Cumberland 20,196 46
Digby 4,464 04
Guysboro 5,521 24
Halifax (City) 110,416 87
Halifax (County) 15,425 31
Hants 10,340 00
Inverness 2,903 67
Kings 10,650 00
Lunenburg 14,446 47
Pictou 40,235 27
Queens 6,341 61
Richmond 2,332 47
Shelburne 7,500 00
Victoria 2,463 38
Yarmouth 11,510 36
Special 1,048 17
Total $343,701 77
Expenses 9,525 37
During the year 1918 the public maintained their interest in the
Prisoners of War Department of the Red Cross. The amount collected
was $20,943.01, of which $19,013.00 was forwarded to headquarters
in London, England, for the support of prisoners. The food rations of
each prisoner of war were despatched regularly three times every
fortnight, which, considering there were 270 men on the list, was no
light undertaking. In addition to the food supply the prisoners
received two complete outfits of clothing, including everything from
shoes and socks to overcoats.
After the signing of the Armistice the work of repatriating
prisoners went steadily on, and each steamer brought men who had
spent many weary months in the prison camps, and they all testified
to the value of the work done by this Society and declared that it was
solely due to this that they were enabled to return to their homes.
This department of Red Cross work has been most ably conducted by
Mrs. Charles Archibald, nobly assisted by Miss Ritchie and Mrs.
Longley.
The beginning of this year was marked by the terrible Halifax
explosion, by which 1,635 persons lost their lives and 10,000 people
were rendered homeless. Much generous help was received by the
stricken city. Everyone knows the splendid aid sent by the American
Red Cross, and the people and Government of the United States in
despatching to Halifax train loads and boat loads of supplies,
together with surgeons and nurses. Their neighborly kindness will
never be forgotten.
But the help rendered by our own Canadian Red Cross is
perhaps not so widely known. The Chairman of the Executive in
Toronto wired to the shipping agent in St. John to render every
assistance possible in money and goods. The agent, Mr. Milburne,
immediately requisitioned a special train, and brought with him all
the Red Cross goods he had ready for shipment Overseas, making
two car loads in all. This train was the first assistance from outside
the Province to reach Halifax. Hearing that some of the injured had
been conveyed to Truro, Mr. Milburne put off cases of hospital
necessaries for their use at that station. A medical supply committee
of the Canadian Red Cross Society was immediately formed with the
sanction of the Halifax Relief Committee, Mr. Milburne being
appointed Chairman, and Mrs. Sexton, Vice-Chairman, with a Staff
of forty-four voluntary workers. Twice daily all the emergency
hospitals were visited and their wants noted and supplied the same
day. The number of these hospitals, dressing stations, etc., amounted
to sixty-two.
At the same time, gifts of clothing, food and money poured in
from Red Cross Branches all over Canada. Ottawa Branch shipped in
one day eight carloads of clothing. The Nova Scotia Branch, under its
President, Mrs. Dennis, co-operated heartily, practically every
Branch and Auxiliary in the Province sending substantial and
generous aid.
Special mention must be made of the work of the President of
the Windsor Red Cross, Mrs. P. M. Fielding, who organized a special
train which arrived the evening of December 6th, bringing doctors
and nurses from Kentville, Windsor, Truro and neighboring towns.
The Windsor Red Cross alone spent $422.74 on Red Cross supplies
and provisions for this trip, Hantsport and other Branches also
providing hampers of food, so that the doctors and nurses had their
meals en route and arrived in Halifax ready to go to work without an
instant’s delay, thereby saving many lives. Mrs. Fielding remained in
the city, established and equipped three dormitories, which
accommodated in all seventy-five nurses. What this meant to the
stricken city will never be computed, and the Red Cross feels that all
who helped can never be sufficiently thanked.
It was not until the end of March that the Red Cross was able to
resume its work for returned invalided soldiers. By that time the
hospital on Pier 2, wrecked by the explosion, had been repaired. The
hospital ships once more made their trips, and the Red Cross
storeroom on the pier was re-stocked. Large requisitions were filled
each month; sometimes only a few hours’ notice was given to get the
supplies on board—sometimes only a few minutes’ notice in the case
of a hospital train. On one trip the ship docked in the morning,
landed her men and went out again with her new supplies in the
afternoon. At another time 1,400 shipwrecked men from S.S. City of
Vienna were visited and supplied with filled kit bags, containing
toilet necessaries, pipes and tobacco. Directly after, the Committee
were called upon to minister to 300 influenza contact cases. This
necessitated the workers going into quarantine for two days, very
busy days, too, the telegrams alone requiring upwards of 300
telephone calls. The Y.M.C.A. and the Knights of Columbus Musical
Club were always at hand to cheer up men who were detained by the
Medical Board, arranging for their benefit concerts, entertainments,
motor drives and teas at private houses.
One thousand seven hundred and sixty-eight cases of goods
were sent Overseas, and the distribution of supplies on this side grew
very greatly during the year owing to the increasing number of
returned men and the opening of new convalescent homes.
The financial statement for the year ended October 31, 1918,
shows receipts of $36,848.65. Expenditures amounted to
$41,804.01, including the following items:
Purchase of materials, City and Provincial Hospitals $12,172
04
Kentville Sanitarium 1,014 42
Soldiers’ Reception Committee 9,000 00
Cigarettes 2,456 51
Soldiers’ Comforts 2,260 23
Libraries 319 79
Nurses 378 10
Furnishings 2,201 36
Clayton Hospital 175 00
Rental and Expenses at Headquarters, 314 3,858 89
Barrington St.
Office Expenses 1,789 85
X-Ray Machines, Kentville 2,396 43
Hospital Ships 823 63
Place. President.
Amherst Mrs. W. R. Fishleigh.
Arichat Mrs. C. D. Terrio.
Antigonish Mrs. D. G. Kirk.
Baddeck Mrs. F. W. McCurdy.
Barrington Mrs. Wilson Crowell.
Berwick Mrs. Alex. Anderson.
Bishop’s Mountain Mrs. C. O. Downie.
Brass Hill Mrs. F. Nickerson.
Brule Mrs. A. C. Cook.
Barney’s River Mrs. Wm. McDonald.
Boulardarie Mrs. J. Fraser.
Canso Mrs. C. O’Donoghue.
Cape North Miss Grace Gwynn.
Chester Dr. C. O. Hebb.
Dartmouth Mrs. W. R. Foster.
Dominion No. 6. Mrs. Anna B. Wight.
Goldboro Mrs. Edgar Silver.
Glace Bay Mrs. P. E. Ogilvy.
Halifax Mrs. Wm. Dennis.
Hazel Hill Mrs. Dunning.
Hantsport Miss Marcia Braine.
Lawrencetown Mrs. D. M. Balcom.
Lawrencetown S.S. Mrs. J. F. Brown.
Moser’s River Mrs. Walter Smith.
Mulgrave Mrs. L. C. Dixon.
New Glasgow Mrs. P. A. MacGregor.
North Sydney Mrs. J. J. Pallen.
Pictou Mrs. A. S. Stalker.
Port Morien Miss C. Macaulay.
Port Bickerton Mrs. George Taylor.
Point Tupper Mrs. James Swaine.
St. Peters Mrs. J. Kemp.
Sydney Mrs. H. A. Nicholson.
Sydney Mines Mrs. B. Archibald.
South Berwick Mrs. G. R. Nichols.
Tancook Island Miss Beulah Wilson.
Trenton Mrs. C. W. Stromberg.
Truro Mrs. Harold Putnam.
West Quoddy Mrs. Alex. Gamnon.
Westville Mrs. R. Fraser.
Windsor Mrs. P. M. Fielding.
Wolfville Mrs. Harold Barss.
Woodlawn Mrs. Jane Nauffts.
Westchester Station Mrs. W. O. Webb.
Yarmouth Mrs. W. D. Ross.
Officers Elected: