Biotechnology and Its Applications

1. The bacterium Bacillus thuringiensis 4. Transgenic models exist for human

produce ____ plants which reduces the diseases except.
amount of ____ used.
(a) Cancer
(1) disease resistant, insecticide
(b) Emphysema
(2) insect resistant, fertilizers
(c) Cystic fibrosis
(3) disease resistant, industrial enzyme
(d) Rheumatoid arthritis
(4) insect resistant, insecticide
(e) Alzheimer’s
2. Given below are two statements:
(f) SCID
Assertion (A) : Transgenic mice are
(1) b, c and d
being developed for use in testing the
safety of vaccines before they are used (2) b and f
on human. (3) c and d
Reason (R) : Transgenic mice are being (4) d, e and f
used to test the safety of polio vaccine.
5. Given below are two statements:
(1) Both (A) & (R) are correct but (R) is
not the correct explanation of (A). Statement-I : The first clinical gene
therapy was given in 1990 to a 4 year old
(2) (A) is correct but (R) is not correct. girl with Adenosine deaminase (ADA)
(3) (A) is not correct but (R) is correct. deficiency.
(4) Both (A) & (R) are correct and (R) is Statement-II : ELISA is based on the
the correct explanation of (A) principle of antigen antibody interaction.
3. Which committee takes decision In the light of the above statements,
regarding the validity of GM research choose the most appropriate answer from
and the safety of introducing GM- the options given below:
organisms for public services? (1) Both Statement-I and Statement-II are
(1) Indian Council of Medical Research incorrect.
(ICMR) (2) Statement-I is correct but statement-II
(2) Genetic Engineering Approval is incorrect.
committee (GEAC) (3) Statement-I is incorrect but Statement-
(3) Indian Institute of Science Education II is correct.
and Research (IISER) (4) Both Statement-I and Statement-II are
(4) Genetic Engineering Appraisal correct
Committee (GEAC)

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6. The advantage of beginning gene 11. The main challenge for production of
therapy prior to birth is- insulin using rDNA techniques was –
(1) The body would not reject it as it has not (1) Splitting A and B-peptide chains
yet recognised 'self'.
(2) Addition of C-peptide to proinsulin
(2) This would give the body plenty of time.
(3) Getting insulin assembled to mature
(3) The cell being extremely young are more form
receptive to gene therapy.
(4) Removal of C-peptide from active
(4) None of these insulin.
7. Cotton bollworm controlled by- 12. Why Bt toxin protein crystal does not
kill the Bacillus?
(1) Cry I Ac, Cry II Ab
(1) Bacteria encloses toxins in special sac
(2) Cry I Ac, Cry II Ac, Cry I Ab
(2) Bacteria are resistant to toxin
(3) Cry II Ac, Cry I Ab
(3) Toxin occurs as inactive protoxins in
(4) Cry I Ab
bacteria
8. Using Agrobacterium vectors,
(4) All of the above
nematode specific genes were introduced
into host plant and these produced :- 13. Which of the following transgenic
human protein products development are
(1) Sense - DNA
used to treat emphysema?
(2) Antisense - DNA
(1) -1 antitrypsin
(3) ds RNA
(2) -1 trypsin
(4) All of these
(3) -1 albumin
9. _____ is removed during the maturation
of proinsulin to insulin. (4) -1 globulin

(1) A-chain 14. Find incorrect statement with respect

to genetically engineered insulin :-
(2) B-chain
(1) 'C' peptide is not present in mature
(3) C-chain insulin
(4) Both (1) and (2) (2) 'A' and 'B' peptide chain produced
10. Bio patent means separately by Eli-Lilly
(1) Right to use an invention (3) Human insulin is produced by plasmid
of transgenic E. coli
(2) Right to use application are processes
(4) Chain A and B, that are linked by H-
(3) Both A and B
bond (hydrogen bond)
(4) None of these

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15. Given below are two statements: (4) A- radiation therapy, B- enzyme
replacement therapy
Statement-1 : RNAi takes place in all
eukaryotic organisms as a method of 18. In reference to genetically modified
cellular defence. plants, identify the correct uses of GM
plant
Statement-2 : This method involves
silencing of a specific m-RNA due to (a) Crops less tolerant to abiotic stresses.
complementary dsRNA molecule that
(b) Reduce reliance on chemical
binds to and prevents transcription of
pesticides.
the m-RNA.
(c) Helps to increase post harvest losses.
Choose the most appropriate answer
(d) Increased efficiency of mineral usage
(1) Both statement I and statement II are
by plants.
correct.
(e) Reduce nutritional value of food.
(2) Statement I is correct but statement II is
incorrect. Choose the correct answer from options
given below :
(3) Both statement I and statement II are
incorrect (1) a, b and c only
(4) Statement I is incorrect but statement II (2) a, c and e only
is correct. (3) b and d only
16. The first transgenic cow, Rosie (4) a, d and e only
produced
19. Cry II Ab and Cry I Ab produces
(1) Human calcium enriched milk (2.4 g/l) toxins that control -
(2) Human protein enriched milk (2.4 g/l) (1) Cotton bollworms and corn borer resp.
(3) Human calcium enriched milk (2.6 g/l) (2) Cotton bollworms and budworms resp.
(4) Human protein enriched milk (2.8 g/l) (3) Corn borer and cotton bollworms resp.
17. Adenosine deaminase (ADA) (4) Nematodes and tobacco budworms resp.
deficiency can be treated by ____ and
____ but it is not fully curative. Here A 20. Golden rice is genetically modified
and B can be crop plant with incorporated gene meant
for biosynthesis of -
(1) A- gene therapy, B- radiation therapy
(1) Vitamin-E
(2) A- bone marrow transplantation, B-
enzyme replacement therapy (2) Vitamin-K

(3) A- organ transplantation, B-hormone (3) Omega-3

replacement (4) Vitamin-A

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 ANSWER KEY
1. (4) 2. (1) 3. (2) 4. (2) 5. (4) 6. (3) 7. (1) 8. (3) 9. (3) 10. (3)
11. (3) 12. (3) 13. (1) 14. (4) 15. (2) 16. (2) 17. (2) 18. (3) 19. (1) 20. (4)

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