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March, 2013/2603
,__-
· B.Sc. (MLT) 1s t
Year Supplcmentar1
. . I

BASIC PRINCIPLES OF BIOCHEMISTRY

Time: Three hours
M. Marks: 75 ..•• .· .
• ~.- , i •
Nofo: Answ~r all questions. .: . ,iy-{;};-: .
'
.
·j •
--,
• ,~ . ,_ .. 1
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Fart-A

/
o~r , Discuss phospholipids and thf:li impSrtant functions 10
q.-2_ Discuss briefly: · ··

fI
.,,,...-- . 2xs.==1 o . ·.:_ ! •
· P (,a) Str.uctu,rqf o._rganization of proteins l" ~0 < • •

~ ~- .:.
.. I- •• • ·" :...·_ • •
r (J-6') Polysaccharides ,' . ·:· :_ . }

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Q.3 Write short notes on:

,{rrfPrecipitation of proteins I_Jy various methods 4

r /'l. J., .
11
•}'--Absorption of carbohydrates '· · - · 4
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p _
P1to~'lsoeloctiic pH_ . · "' · _ •
0

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~rUh ). d.Mt'~ 4_,.,_/1-41i. ll'1 B ~ ,.La.,. • u.,/ t,~. ~
aj 1
-~ ·21 What is the 'buffer? Expla h various, typ¢'t, of bHffirs ..ll~~d. ~t'i .- -10 . ... . i • ..

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Q-:-2" (a) Define radio isotopef. "Explain the use of radio isotopes in , Ct9\abt1(J:d""t,J
-----v - . ✓
biochemistry. _

.
1,'f (b1Donnan membrane ;quilibrium.
. 5
(cj Explain various s ifety m2asures required to be followed in
'; 5
bi()~~e:r::stry la.h ratory.

\.V:ite- shcrt notes 0.1:

5x3=·15
fa1ivlbraiity and normalitt
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 Roll No ...... f~.~:=:~.~·-• ......
Oct, 2013/2603
I a. Sc. (MLT) 1 st
Year
BASIC. PRINCIPLES OF BIOCHEMIS
fRY
Time: Three hours
M. Marks: 75
Note: Answer all questions.

Part-A ~
;Q.1) / Mention • the principle, procedure ·
and observation .of positiv_e
V results for qualitative biochemical tests for ca,rbohydra
t~s.
1O

Q.2 Write short _notes on any five:

5x5==25
~) Ctassificapon of lipids with examp
les
..Jp) Different biochemical tests to·evaiuate quality·
fats of
(c) Dige~tion and absorption of dietary
carbohydrate
(d) Structur.e ·ot prot~ins . I
I
-~ ) Polysaccharides i
I

~ Phospholipids.
I

/
(g) Digestion and absorption of dietary fats
/
J.
Par t-8

Q...1. . Write In detail about laboratory hazar;d

. s and varipus steps to be
taken to combat it. •,
. 1O

-
Q.2 Defir,i~. standard deyiatiorz{§.91 Mention
medical 9iochemis.t iy lab ora to~
• ,. I
J •
.
it~ various applications in
. .
5
What do you me
9n by normal s~lim I Mention its uses in
laboratojy. Writ~1tl:Je steps to prepare 5 '
50( nl of normal ·salin~. ·.
\
Q.4
' .
Write short note on any four : 4x5==20
~) Laboratory waste disposal
·¥..;,J
(b) Buffer solutions
~) Anticoagulants
~) PreseNation of biological samples
ctLr / tvtl
(e) Principle and uses of spectrophok
neter
~R ou tin e qualit~tive analysis of urin
i
• V

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 ()1Li-os-

Roll No .. ./.6.f..$1.)..$. ... Oct, 2014/2603

l.;I T) 1st Year
8. Sc. (ML_

BASIC PRINCIPLES OF BlO9HEMISTRY

Time: Three hours· M. Marks: 7fJ

Note: Answ~r all questions.

Part-A

qj Explain the st!ucrure ·- ·a nd-properti~s c ( bl61ogic~I .memp.ran~s~.- · 10·

Enumerate various cell organelles and give _qne important function
for each organelle. . . .
~

Q.2 . Write short notes on any five: / 5x5=25

(a) Sorting of biomedical waste 1 . .
(b .olleGtion arid preservation of urine samples / .
Advantages and disadvanfages.ofusirig universal indicatof ;;_
Optical rotation of compounds ·. , • . •'<•
(.e) Universal precautions ' '
(f) .Sources. ...
and prevention of radiation hazard
•,•

Part-8

Q."3 Define lipids and enumerate their functions. Add a note on

· cta~ification ·and composition of compo,und lipids with suitable· 10
exampfes. ·
Define reference range of an analyte in a biological fluid. Write the
reference range for plasma glucose (fa~ng and post prandial),
blood urea, serum Creatinlne, serum sodium and potassium. .
. .,
o.5 Define molarity and normality of a solution . . Write the steps for 5
preparaing 500ml· of 4 molar solutiotJ of NaOH.~
o., I

Write short not~s on any 4: ·

/

5x4=;:=20 ·
(a) Similarities and differences between- .colori_meter. and
spectrophotometer .
,J(b) Characteristics of.a normal curve . .
(c) lsoelectric· plj .of proteins and its practi~al 'applicati.o.ns
(d) Principle and 9pplication of biuret test · .
).€) Use .of enzyme in diagnosis of liver dis~ases
..
 Roll No.P.-.'J.f.~1.~ ........ . Oct, 2016/2603
B. Sc. (MLT) 1st Year-Annual, 2016
BASIC PRINCIPLES OF BIOCHEMISTRY
Time: Three hours M. Marks: 75
Note: Answe r all questio ns.

Part-A
Mention th~ principle, procedure and observation of positive results - 10
for qualitat
. . -··-ive biochemical tests for£arbohydrates .
Q.2 Write short notes on an~ fi :e. 5x3=15
"''{a) Structure of proteins ·
"{b) Polysaccharides ·
~) Phospholipids
•'
'·,
Q.3 Write short notes on: 5x2=10 .
~ '-{a) Essential fatty acjds-
. 'it>) lsoelectric pH-:- '
Part-B

Q.4 Write in detail about laboratory hazards and V?rious st~ps. to be.
taken to comt;>at il 10
Q.5 What do you· mean by no·rmal saline? Men~ion. its uses in : 5
laboratory. Write the steps to ·prepare 500ml of normal .saline.
. _,....,/ - ----- -
Q.6 Write short notes on: ,,....--/ ·sx5=25
(a) Carcinogens
(b) Preparation of EDTA vial .
. .
.... (c) Similarities · and differenc~s between colorimeter . and·
spectrophotometer .
(d) ·Principle and application of biuret test
. ;(e) Buffer solutions ;.

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.... •.F.- -··.' J"--o'
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 ·Roll No,;S_.~_75.gl Sep, 2017/2603
· B.Sc. (MLT) 1st Year-Annual, 2011
BASIC PRINCIP~ES OF BIOCHEMISTRY
Time: Three hours M. Marks: 75
Note: Answer all questions.

Part-A
~ Describe various isomerism in carbohydrates. 10
~ Write short notes on:
5x3=15
• ~a) Oenaturation of proteins ·
_~) Phosp_holipJ.ds _ _ _ .. __ ..
.-. ~·-~, ,:~·· .~ ~lycoside~ ~nd the_ ir importa~ce. "·=,.-•,,._ ....

~rite s~ort notes on:

::ia) lsoel~ctric pH .
j\)) Lipid peroxidation
...
•
2 ½x4=10

~) Polysaccharides
~)' Essential amir\9 acids

Part-B

Write in detail about general safety measures to be followed in

clinical bioc~emistry laboratory. ~ 10
Write briefly on:
< •
5x3=15
~) Method of collection and preservation of blood
, ~ Protein free filtrate
~) Cleaning of glassware .

Q.6 ::W~rite short notes on: 1S,~01ld\ . .

Write normal values of urea, u~id and creatinine in blood 3xs=1s
What is proteinuria and enum·erate various causes of
'\. .proteinuria? ·
(c} Explain why creatinine clearance is better kidney function test
than urea clearance ·
(ti) Whai do you mean by direct and indirect bilirubin? Write· its
· "\. significance in diagnosis ot jaundice?
(~ What is <;,TT and briefly explain the procedure for doing this
test?

.
!

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~f - --:: . - / : ] ~ ~ ~ •
"f- -
 Sent up Examination
B.Sc. l\1LT 1st Year
\
Biochemistry
Time: 3 hrs
Max. Marks: 75 '\
Part-A, . ·
~scuss phospho!ipids and \heir important fu~cti~ns. I
8
o/Explain the following : . ·
4x4 = J6
/ a) Role of isotopes in.medicine
.,..b) Preparation of IN-H2S0 4
I IJ

c) Safety .rneasures for accidents in laboratory

d) s.r. units

Q.3 Discuss the foilowing:

4x4=16
. a) Collection and presentation of urine
,.-b) Advantages and disadvantages of urine unjyersal indicator ~p{

~~
c;) Different types of pipettes
@simple and analvtical balancl! . \:

Part-B

Q.4 a) What is the buffer? Explain various t, pes of buffers used in biochemistry lab.
7
b) Explain various types o·f glass~are, 3ed in laboratory. Discuss tbe cleaning,
care and ·maintenance ofglassware 7

Q.S·Explain briefly:
..
4 X 3 = 12
. a) Donnan inembrane equilibi Lim
b) Mo~ality and normality
, c) I,,ambc:_It-Beer law ·
"' @)Bcnedi~'s test ·

Q.6 Write notes 011 :

3x3;=,. 9
' a) Mean 2nd standai~d de1 iation

.@ Give.on account of preparation of p1 iJcin·free filtr, te of blood

. -
~
1

.••t) Disc~-~.~cg·iQ~l~ P~5~~ns to be ta]. ~n _to minimi: infectiops_ind~boi·~tory workers

• ' • ' , , "' ' • y -.. , • .,.;, - - • • . !.~-' ,.. . ...,..
. ... ~
 Biochemistry
tr
I
I
MsX· 1\1arks = 50 Time= 2 hrs

Q.l ,vrite the primary role of a 1!1edical lab technologisi along w:th 8
ethics and responsibilities.
\
~-~\'

Q.2 \Vrite first aid & emergency treatment in thermal injury. 7

Q.3 \Vhat is the difference between serum and plasma? Why serum is 7
.preferred over plasma?

Q.4 Expl('lin the use of various anticoagulants used in sample collection. 7

Q.5 What is standard deviation (SD). Illustrate the Sc!-me giving example. 7
'

Q.6 For interpretation of results. What are the reference values and normal 7

(reference) range?

Q.7 Describe corrosive chem1cals and their cffe ts on living tissues. 7 .

• .t.- 3 -
Lil£L"r,G3.,""UQll'S~.1'-~ ,:._;e e
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 Sent ~p Examination

B.Sc. MLT 1st Year

l• . Biochemistry
1

Time: 3 hrs. Max. Marks: so

•Part-I
·. . 'fiicance of compound lipids
Q.I Whnt are lipids. Classify them. Describe ilie sigm 8
with example.

Q.2 Discuss: 2x 4== 8

a) Polysaccharides
b) Conjugated proteins and·their significance.

C. Q.3 Write short notes on:

a) Acid base balance .
b) Role of isotopes in diagnosis and treatment of disease. ...
'
c) Digestion and absorption of carbohydrates

- Part-II ·
I ••

. . .
Q.4Write the first aid and emergency trea:tmenf in chemical injury. Describe the safety 8
( _. ·. measures for the storage of explosive and flammable chemicals. ·

Q.5 Discuss:
◄

a) Preservation and di~po~al o_f biological sample. 2x4 = 8

b) Role of medic~l lab tecbnologis~ in he_a lth care system.

Q.6 Write short notes on:

a) Units of tneasurement in clinic'a.l chemistry 3x3= 9
b) Coeffi~ient ofvariatibn (CY) .
c) Differentiate between normal range and refere~ce range. ·
 Ti111e: 2 hrs
Max. Marks== 50
II

I
I

Ql. Define carbohydrates, classify them with exarnples. Describe in brief various 15
properties of carbohydrates.

Q2. Describe:
5X 4 = 20
a) Role and ethics of a medical laboratory technologist.
. . J
b) Explain omega and delta systems of nomenclature of fatty acids. ~ t are
essential fatty a9ids.
c) Structural conformation of proteins.
r
d) Heteropolysaccharides
,-
& their significance.

Q3. \Vrite briefly about:

5 X 3 = 15
a) What is normal' saline? I-low will you prepare 200ml of rionnal saline.··
b) Role of isotopes in medicine & diagnosis.
c) '\Vhat is osmosis. Describe the process o_f donnan membrane equilibrium.

.
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 B.Sc. YlLT 1st Year
l -
I· B_:ochcmistry ·
I

)
I Time= 2 hrs
Max. Marks= 50

·- 10
) Q. l What are carbohydrates. Classify them. Differentiate between
homopolysaccharides and heteropolysaccp~rides

Q.2 Briefly describe: 5 each

a) PhospJ:iolipids and their signifj.cance. t

I
b) Lipoprotein{.

- Q.3 Discuss: 5 each

a) Structural organisation of proteins
b) Precipitation, denatur;tion and coag~lation.

Q.4 Discuss briefly: -

5 each
a) Digestion and absorption of carbohydrates·
b) Various fun~tions of proteins

Q_.S Write short notes on:

2.5 each
a) Essential anjino a~ids.
b) Diffusion

c) Donnan membrane equi·]ibri~rri

d) Significance of radioisotopes in medicine
 •

B.Sc. M.Lt 1st Year

Examination
Bioch_t,mistry
Max. l\1arks :::: 50
Time: 3:00 hrs ·

Q.1 What are lipids. Classify them. Descripe the significance of compound lipids 8
' with example.

· Q.2 Dehne radioisotopes. Explain the diagnostic and the ·apeutic uses <if radioisotopes. ·
Also describe the hazards of radioisotopes in bioch, .misti-y. · 8
Q.3 Write notes on:
-i) Absorption of carbohydrates 3x3=9
ii) Buffers
iii) Precipitation reaction of protein

j '
fart]I [ 8,ac~d~i]
-
,..Q_)l--Discuss the principle· of centrifugation a• .d different types of centrifuges used in
laborato1ies. 8
. ., ,

g,, Explain various tyj,es of_J?;!asswares

and maintenance of glassware.
1 sed in laboratories. Discuss th.e cleaning, care
• 8

Q.6 Write notes on: 3x 3 ~9

i) Hygroscopic chemicals .
il) Feccal occult blood ·

~) D;fine molarity-;;,,d n?n iality of a {c)iuti0\1, Write the steps for preparing
500ml of 4 molar solutiq ofNaOH. \. '.} \ · . .
/ ,
0 fr),
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l-S == l,5 I/ .
1· ~:
 MAHARAJ A AGRASEN MEDICAL COLLEGE, AGROHA (HISAR)

B.Sc. MLT 1st year Theory Examination

BIOCHEMISTRY
MIX l'vldf'kS 75 Time: 3 hours

PART A

l. (a) Define lipids. Classify them with suitable examples 5,5,4

(b) Describe colour reactions of carbohydrate.
(C) Use of rcJdioisotopes in medicine.

2. Discuss the following

(a) Explain strength of a solution and various ways to express it. How wfll you prepare
500 ml of IN-sodium hydroxide solution'? 3,5,5
(h) Principle of Centrifugation anci different types of centrifuges used in laboratory.
(c) Discuss VZlrious types of apparatus uc,ed 111 measuring volume in laboratory.

J Write Short notes on the follovving : 4,4,3,2

(a) S.1. Units
(b) Simple and analytical balance.
(c) Laboratory first-aid kit.
(d) Use of Rrown bottles in l:1boratory

PARTB

4 ( ,1) Define pl I. What nre the var·ious methods of its measurement in laboratory? 5, 5
(b) What arc the dlmormal con:;tttuc11ts 01 urinL'? Write the pathophvsiology of each
llJtlSlttlll'lll

S. (a) The V.iluc ol Serum lit et1 ol l SO pc1l1L'tlts ,lrt' l,1bul<1tL>d bL•low: 5,5,3
Urea (mg/di) :0- l U I 0-20 20-30 :rn-.io 40-50
No. of l\1Lients : 17 :rn 2fl 70 5
C1lrnlc1te tlw c1vc-r.igc sernrn urcc1 vctlUL'.

(b) l)rsrns<., the prrncipll ,111el '>1g11tf1c,111ce ul 1)1.ilv'>t~.

1

( r) .\:iritc down ti'.~ 1_101 m,11_ t ,lllgt> of /\lk;l1111e pl10sµlic1tc1se, AspclrcHe tr,rnsrninase,
I ot.11 protetn, I nglyccndc, Creatinine c111d Tot,,! bilirubin in serum.

6.
\Vli,tt is the clinic1I s1gnilic<111le ot C:liluricle cstim,1tion'? llo1,,v is it estimated in
I ,1ho1 ,1tu1y .' ~ ,
(Ii) \V1 tlL' c1 '>IH>tt 11ntL' 011 '>tor·c1•~c: of cheni1t ,llc., 4-,4, i
(l l \'v I rte po~'>1ble Ii 11.cll'dc., 111 IHoll1l'mist.- !.ih
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N ):e A n swer al! que::.t:0ns

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<= f
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I )I <J'Ci:lt 't.nctions of ro, 1 Ji~;-c:l a €•"" ' ·:· 1 I .r•o .

.:'(2,) Wrttu c. l1_ort no!_es 0_0~

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I ✓I I , 1 , 1 id 1n cl1n1c al ~c:--iem1st~? lat,
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 B.Sc. MLT 1st year Theory Examination
)

Max. Marks :50 marks B10-CI-tEMISTRY

Time: ·2 Hours
,,
Q. 1 Discuss collection & preservation of Biological Samples. What a the ·
methods of their safe disposal? (10)

Q. Z What are anticoagulatnts. Describe their types and uses in detail. (10)
Q. 3 Write notes on: - 6X5=3r)

a. Calibration of volumetric apparatus.

~. -- -
t --
Role of Medical Lab Ted,inologist.
- l l •

c. Difference between normality and TI1ol~1rit~,

d. Hygroscopic and light sensitive reagents.
----------
..e. Standard deviati~n and its application.
f. First aid and emergency treatm•~nt in thermal injury.
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