MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Course Name: Biochemistry
Mode: Offline Credits: 5(3T+2P)
BMBT1101
Aim of the Course: The aim is to achieve knowledge of the fundamentals of biochemistry and basic
knowledge of metabolism
Course Objectives: The course is aimed to build knowledge in fundamentals of Biochemistry, their
relation to the metabolisms. It also considers different structural and reaction of different simple
macromolecules. Upon completion of this course, students should understand the structural,
functional and metabolic aspects of carbohydrates, lipids, amino acids and proteins along with nucleic
acids, . They should understand the structure and functional details of biological membranes.
Sl Graduate attributes Mapped
modules
CO1 The student will get an introduction to the discipline of biochemistry and M1
structural organisations of carbohydrates and different reactions etc.
The students will get a clear concept on structure and properties of
CO2 Amino acids & Proteins, M2
The student shall get a concept of lipids, Biological membrane, and the
CO3 Membrane Transport. M3
The details about Biologically important nucleotides are the knowledges
CO4 students will learn. M4
The student shall master the Enzymatic Kinetics, including reaction rates
CO5 and mechanisms. The student will be able to analyze reactions of M5
enzymes.
The students will understand carbohydrate , lipid , amino acids,proteins
CO6 and nucleic acid metabolisms. M6
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Learning Outcome/ Skills:
The candidates should demonstrate fundamental knowledge and insight into Biochemistry in order
for the candidate to be able to understand and solve problems related to the macromolecular
structures, their function and importance.
Knowledge and understanding should be demonstrated in the areas of: (1) carbohydrate, (2) lipid (3)
amino acids, (4) nucleic acids (5) structures, (6) metabolisms and related knowledges.
Module Content Total % of Bloom Level Remarks, if
Hours questions ( applicable) any
Number
THEORY
M1 Historical perspectives, and 5 7 1,2 NA
carbohydrates
M2 Amino acids and proteins 7 15 1,2,3 NA
Lipids and biological membranes
M3 5 8 1,2 NA
Nucleic acids Double helical model
M4 of DNA 5 10 1,2,3 NA
Chemical and Enzymatic Kinetics
M5 8 20 2,3,4 NA
Metabolisms
M6 15 40 2,3,4 NA
Total 45 100
Theory
Practical
30
TOTAL
75
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Detailed Syllabus
Module 1:
Introduction to Biochemistry
Introduction to Biochemistry and carbohydrates-
A historical prospective.
Carbohydrates:-–
Introduction & Occurrence, Classification of Carbohydrates, Determination of Structural of Glucose and
Fructose, Pyranose and Furanose forms of Glucose, Reducing & Non-reducing Sugars, Oxidation and
reduction reaction of glucose and Fructose, Reaction with Phenylhydrazine, Epimerization, Mutarotation,
Ascending and Descending the Sugar series, Inter-conversion of Monosaccharides, Determination of ring size
of Carbohydrate.
(Total Hours: 5
Module 2:
Amino acids & Proteins:
Structure & Function. Structure and properties of Amino acids, Types of proteins and their classification,
Forces stabilizing protein structure and shape. Different Level of structural organization of proteins, Protein
Purification. Denaturation and renaturation of proteins. Fibrous and globular proteins.
Total Hours: 7
Module 3:
Lipids and Biological membranes
Lipids : Structural aspects – General introduction, Classification & Structure of Simple & Compound lipids, Properties of
Lipid aggregates (elementary idea), Biological membrane, Membrane protein – structural aspects, Lipoproteins
(elementary idea).
Total Hours: 5
Module 4:
Nucleic Acids:
Nucleic acids: Structure and functions: Physical & chemical properties of Nucleic acids, Nucleosides &
Nucleotides, purines & pyrimidines,. Biologically important nucleotides, Double helical model of DNA
structure and forces responsible for A, B & Z – DNA, denaturation and renaturation of DNA
Total Hours: 5
Module 5:
Chemical & Enzymatic Kinetics
Chemical & Enzymatic Kinetics - An introduction to enzyme; How enzyme works; Reaction rate; Thermodynamic
definitions; Principles of catalytic power and specificity of enzymes, Order of reactions; Enzyme kinetics – Approach to
mechanism.
Total Hours: 8
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Module 6:
Metabolisms
Carbohydrates Metabolism: Reactions, energetics and regulation. Glycolysis: Fate of pyruvate
under aerobic and anaerobic conditions. Pentose phosphate pathway and its significance, Gluconeogenesis,
Glycogenolysis and glycogen synthesis. TCA cycle, Electron Transport Chain, Oxidative phosphorylation. ß-
oxidation of fatty acids.
Lipid Metabolism – Structures and roles of Fatty acids & Glycerols, beta oxidation of saturated
fatty acids, oxidation of unsaturated fatty acids, oxidation of odd chain fatty acids, energy yield, Ketone
bodies.
Amino acid Metabolism – Amino acid breakdown (amino acid deamination, Urea cycle, metabolic breakdown
of individual amino acids – glucogenic & ketogenic amino acids), amino acids as biosynthetic precursors
(haem biosynthesis & degradation, biosynthesis of epinephrine, dopamine, seretonin, GABA, histamin,
glutathione); biosynthesis of essential & non-essential amino acids.
Nucleotide Metabolism – biosynthesis of purine & pyrimidine (de novo & salvage pathway); degradation of
purine & pyrimidine.
Total Hours: 15
PRACTICAL
Credit: 2
Total Hours: 30
BMBT1191
[Lab on Biochemistry and Metabolism] Credit 2 (Wherever wet lab experiments are not possible the principles and
concepts can be demonstrated through any other material or medium including videos/virtual labs etc.)
1. Estimation of protein by Folin Lowry method
2. TLC separation of Amino acids /sugars
3. Determination of Iodine number of a fat
4. Estimation of DNA by diphenyl amine method
5. Qualitative tests for Carbohydrates, lipids and proteins
6. Testing of Blood Sugar
7. Testing of Liver Function Test (Bilirubin, SGOT, SGPT, Alkaline Phosphatase, Albumin,
8. Testing of Renal Function Test (Urea, Uric acid, Creatine, Creatinine)
Suggested Readings:
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman and Co.
2. Buchanan, B., Gruissem, W. and Jones, R. (2000) Biochemistry and Molecular Biology of
Plants.American Society of Plant Biologists.
3. Nelson, D.L., Cox, M.M. (2004) Lehninger Principles of Biochemistry, 4th Edition, WH Freeman and
Company, New York, USA.
4. Hopkins, W.G. and Huner, P.A. (2008) Introduction to Plant Physiology. John Wiley and Sons.
5. Salisbury, F.B. and Ross, C.W. (1991) Plant Physiology, Wadsworth Publishing Co. Ltd.
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Course Name: Cell Biology
Mode: Offline Credits: 5(3T+2P)
BMBT1102
Aim of the Course: The aim is to achieve Basic knowledge of the fundamentals of cell structure and
basic knowledge of different functions.
Course Objectives: The course is aimed to build knowledge in fundamentals of cell Biology, the
basic concepts of the components of prokaryotic and eukaryotic cells. It also considers how cells
function. Upon completion of this course, students should understand the basic knowledge underlying
cell cycle and develop an understanding of apoptosis or cell death. They should be able to
characterize properties of different component of cell systems and relate them with different functions
Sl Graduate attributes Mapped
modules
CO1 To acquaint students with basic knowledge of Cell Biology, Historical M1
perspectives- Discovery of cell and Cell Theory
CO2 Students will learn about the structure and organization of Prokaryotic Cell and M2
different cellular organelles.
Students will get a concept on Comparison between plant and animal cells, cell
CO3 compartmentalization, cell fractionation as well as the basics of Cell Membrane M3
and Permeability .
The student shall get a detailed concept of structure, function biogenesis of
CO4 Membrane, Vacuolar system, cytoskeleton and cell motility, microtubules, M4
Microfilaments, Intermediate filaments. ER, Golgi complex etc.
Students will be acquainted with the structure, function and biogenesis of
CO5 Lysosomes and microbodies, Structure and functions Ribosomes,. M5
Mitochondria. Chloroplasts, Nucleus
The student shall master the knowledge about Eukaryotic Cell cycle and its
CO6 regulation; cell division, Programmed cell death, Apoptosis and its relation M6
with Cancer.
Learning Outcome/ Skills:
The candidates should demonstrate fundamental knowledge and insight into cell biology and the will
will be also able to understand problems related to the structure of cell organelles, their functions
and importance.
Knowledge and understanding should be demonstrated in the areas of: cell membranes , their
permeabilities, cell divisions and apoptosis related knowledges.
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Module Content Total % of Bloom Level Remarks, if
Hours questions ( applicable) any
Number
THEORY
M1 Basic knowledge of Cell Biology, 3 6 1 NA
Historical perspectives- Discovery of
cell and Cell Theory
M2 Structure and organization of 7 10 1,2 NA
Prokaryotic Cell and different cellular
organelles.
Comparison between plant and
M3 animal cells, cell 5 14 1,2,3 NA
compartmentalization, cell
fractionation, Cell Membrane and
Permeability
Structure, function biogenesis of
M4 Membrane, Vacuolar system, 9 20 1,2,3 NA
cytoskeleton and cell motility,
microtubules, Microfilaments,
Intermediate filaments. ER, Golgi
complex.
Structure, function and biogenesis
M5 of Lysosomes and microbodies, 9 25 2,3 NA
Structure and functions
Ribosomes,. Mitochondria.
Chloroplasts, Nucleus
Eukaryotic Cell cycle and its
M6 regulation; cell division, 12 25 2,3,4 NA
Programmed cell death, Apoptosis
in relation with Cancer
Total 45 100
Theory
Practical
30
TOTAL
75
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Detailed Syllabus
Module 1:
Basic knowledge of Cell Biology
Basic knowledge of Cell Biology, Historical perspectives- Discovery of cell and Cell Theory
Total Hours: 3
Module 2:
Structure and organization of Prokaryotic Cell
Structure and organization of Prokaryotic Cell: Prokaryotic Cell size, shape and arrangement, glycocalyx:
capsule and slime layer, flagella, endoflagella, fimbriae and pili. Cell-wall: Composition and detailed structure
of Gram-positive and Gram-negative cell walls, Gram staining mechanisms, Unique cell wall structure of Acid
fast bacteria, sphaeroplasts, protoplasts, and L-forms. Composition of Archaebacterial cell wall and cell
membranes. Cytoplasm: Ribosomes, mesosomes, inclusion bodies, nucleoid, chromosome and plasmids.
Endospore: Structure, formation and stages of sporulation.
Total Hours: 7
Module 3:
Comparison between plant and animal cells
Comparison between plant and animal cells; cytosol, compartmentalization of eukaryotic cells, cell
fractionation. Cell Membrane and Permeability: Chemical components of biological membranes, organization
and Fluid Mosaic Model, membrane as a dynamic entity, cell recognition and membrane transport. Cell wall;
Plasma membrane; Modification of plasma membrane; Extra cellular matrix and cell matrix interactions, Cell-
Cell Interactions - adhesion junctions, tight junctions, gap junctions, and plasmodesmata (only structural
aspects); Protoplasm.
Total Hours: 5
Module 4:
Membranes and organelles:
Membrane, Vacuolar system, cytoskeleton and cell motility : Structure and function of microtubules,
Microfilaments, Intermediate filaments. Endoplasmic reticulum: Structure, function including role in protein
segregation. Golgi complex: Structure, biogenesis and functions including role in protein secretion.
Total Hours: 9
Module 5:
Ribosomes, Mitochondria, chloroplast, Nucleus
Lysosomes: Vacuoles and micro bodies: Structure and functions Ribosomes: Structures and function
including role in protein synthesis. Mitochondria: Structure and function, Genomes, biogenesis. Chloroplasts:
Structure and function, genomes, biogenesis, Nucleus: Structure and function, chromosomes and their
structure.
Total Hours: 9
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY, WEST BENGAL
(Formerly West Bengal University of Technology)
Syllabus of B. Sc in Biotechnology
(Effective from 2023-24 Academic Sessions)
Module 6:
Cell cycle, Cell division, Apoptosis
Eukaryotic Cell cycle and its regulation; cell division -Mitosis and Meiosis; Programmed cell death
(Apoptosis), intrinsic & extrinsic pathways of cell death, Apoptosis in relation with Cancer.
Total Hours: 12
PRACTICAL
Credit: 2
Total Hours: 30
BMBT1192
[Lab on cell biology] Credit 2 (Wherever wet lab experiments are not possible the principles and concepts can be
demonstrated through any other material or medium including videos/virtual labs etc.)
1. 1 Preparation of Mitotic Chromosome from onion root tip.
2. Preparation of Meiotic Chromosome from Rhoeo discolor or onion sp.
3. Preparation and study of polytene chromosome from Drosophila salivary gland.
4. Study of sex chromatin through preparation of Barr body from buccal epithelium .
5. Study of plasmolysis and de-plasmolysis.
.
Suggested Readings:
1. Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th edition,Pearson
Benjamin Cummings Publishing, San Francisco
3. The Cell: A Molecular Approach (7th Edition), Geoffrey M. Cooper, Robert E. Hausman.
4. Essential Cell Biology, Bruce Alberts, Dennis Bray, Karen Hopkin, Alexander D.Johnson, Julian Lewis, Martin
Raff, Keith Roberts, and Peter Walter.
5. Cell Biology, Genetics, Molecular Biology, P.S.Verma
