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Hudson K - DNA Structure and Replication POGIL

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188 views5 pages

Hudson K - DNA Structure and Replication POGIL

Uploaded by

hudstud26
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DNA Structure and Replication

How is genetic information stored and copied?


 
Why?
Deoxyribonucleic acid or DNA is the molecule of heredity. It contains the genetic blueprint for life. For
organisms to grow and repair damaged cells, each cell must be capable of accurately copying itself. So how
does the structure of DNA allow it to copy itself so accurately?

1. Refer to the diagram in Model 1.


 

a. What are the three parts of a nucleotide?


 
 
 
  phosphate, Nitrogen-containing, deoxyribose sugar
b. What kind of sugar is found in a DNA nucleotide?
 
  deoxyribose sugar
 
c. Which nucleotide component contains nitrogen?
 
  the base
 
d. Name the four nitrogen bases shown in Model 1.
 
 
  adenine, thymine, guanine, cytosine
 
 
  DNA Replication 1
 
2. DNA is often drawn in a “ladder model.” Locate this drawing in Model 1.
a. Circle a single nucleotide on each side of the ladder model of DNA.

b. What part(s) of the nucleotides make up the rungs of the “ladder”?


  nitrogen base
 
c. What parts of the nucleotides make up the sides (backbone) of the “ladder”?
  phosphate and sugar
 
d. Look at the bottom and top of the “ladder” in Model 1. Are the rungs parallel (the ends
of the strands match) or antiparallel (the ends of the strands are opposites)?
 
anti-parallel
 
3. On the ladder model of DNA label each of the bases with the letter A, T, C or G.
4. Refer to Model 1. When one nucleotide contains adenine, what type of base is the adenine
attached to on the opposite nucleotide strand?
  thymine
 
5. The two strands of DNA are held together with hydrogen bonds between the nitrogen bases.
These are weak bonds between polar molecules. How many hydrogen bonds connect the two
bases from Question 4?
  2
6. Refer to Model 1. When one nucleotide contains cytosine, what type of base is the cytosine
attached to on the opposite nucleotide strand?
 
  guanine
7. How many hydrogen bonds connect the two bases from Question 6?
 
3
 
8. With your group, use a complete sentence to write a rule for how the bases are arranged in the
ladder model of DNA.
 
  The two strands are anti-parallel and the when one strand has adenine the other side
  has thymine and when one side has cytosine the other has guanine.
 
Read This!
Erwin Chargaff (1905–2002), an Austrian-American biochemist, investigated the ratio of nucleotide bases
found in the DNA from a variety of organisms. From his research, as well as research by Rosalind Franklin
and Maurice Wilkins, Watson and Crick developed the complementary base-pair rule during their
race to discover the structure of DNA. The complementary base-pair rule states that adenine and thymine
form pairs across two strands, and guanine and cytosine form pairs across two strands.
 
9. Fill in the complementary bases on the strand below according to the base-pair rule.
A T C C A G
 
  T A G G T C
10. The ladder model of DNA is a simplified representation of the actual structure and shape of a
DNA molecule. In reality, the strands of DNA form a double helix. Refer to the double
helix diagram in Model 1 and describe its shape using a complete sentence.

  2 for Biology
Model 2 – DNA Replication

 
11. Examine Model 2. Number the steps below in order to describe the replication of DNA in a cell.
 
4 Hydrogen bonds between nucleotides form.
 

1 Hydrogen bonds between nucleotides break.


 
2 Strands of DNA separate.
 
3 Free nucleotides are attracted to exposed bases on the loose strands of DNA.
 

12. Locate the DNA helicase on Model 2.


 

a. What type of biological molecule is DNA helicase?


 
  enzyme
 
b. What is the role of DNA helicase in the replication of DNA?
 
 
to break the hydrogen bonds
 
13. DNA polymerase is another important enzyme in DNA replication. DNA polymerase adds
free nucleotides to exposed bases on the loose strands of DNA. Indicate on the upper strand of
DNA in Model 2, where DNA polymerase would be active.

14. What rule is used to join the free nucleotides to the exposed bases of the DNA?
 
  base-pairing rule
 
 
  DNA Replication 3
 

15. This type of replication is called semi-conservative replication. Considering the


meaning of these words (semi—half; conserve—to keep), explain why DNA replication is called
semi-conservative.
each new strand is using half of the old strand

16. DNA molecules can be tens of thousands of base pairs in length. Mistakes in DNA replication
lead to mutations, which may or may not be harmful to an organism. How does semi-conservative
replication help prevent mutations during DNA replication?
 
  it uses pre-existing strands that don't contain problems
 
 
 
 
 
17. The proportions of the bases are consistent within a species; however they do vary between
species. Using the base-pair rules, complete the following table to show the percentage of each
type of base in the five different organisms.
 

19 31

22 28

29 21
23 23 27
19 31

  4 for Biology
Extension Questions
Model 3 – Timing of DNA Replication

18. According to Model 3, what term refers to loose DNA inside of a nucleus?
 
 
 
19. During what part of the cell cycle is the DNA in a cell’s nucleus replicated?
 
 
 
20. During what part of the cell cycle is the DNA in a cell condensed into chromosomes?
 
 
 
21. Replicated chromosomes are often illustrated as an X shape to match how they look in real life
just before cell division.
 

a. According to Model 3, which of the following diagrams correctly show an original set of
homologous chromosomes (grey) and their sister chromatids (black)—the replicated portion?
 
 
 
 
 
 
b. What structure holds the two sister chromatids together as they prepare for cell division?

  DNA Replication 5

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