Introduction to Antimicrobial packing film
from Bio materials:
Nishank Das[21BCM0093] Ankit Mishra[21BCM0133]
Introduction to
Antimicrobial
Packing Film
The purpose of this project is to demonstrate how biofilms
are produced from bagasse, or sugar cane waste, and to
evaluate and apply anti-microbial capabilities by using
acidulants and essential oils of turmeric.
Background
1 Cellulose 2 Biofilm:-
Nanocrystal : - The food packaging,
electronics, coatings,
Cellulose Nanocrystals and biomedical
industries can all
are rod like
benefit greatly from
nanoparticles the use of bio-films
originates from the made from cellulose
nanocrystals, which
structural component are created by the
of plant cell walls , casting process.
cellulose . They are
crystalline in nature
and are the most
abundant natural
polymer on Earth.
Objectives of the Project
The primary objectives of this project are to develop and optimize a biomaterial-based antimicrobial
packing film, focusing on the following aspects: -
Objective 1:- Objective 2:-
To create a biofilm from sugarcane waste, evaluate its To determine antimicrobial qualities and
characteristics, and compare it to other biofilms made compare the various antimicrobial agents that
of related materials. are utilized.
Methodology
EXTRACTION 1
2 ALKALI TREATMENT
BLEACHING 3
4 DRYING
FTIR ANALYSIS 5
s used to check the content of cellulose, hemi-cellulose and ligmen using chemicals such as NaOH and NaOCl.
6 THERMAL PROPERTY ANALYSIS
Thermal property analysis of antimicrobial packaging films from biomaterials examines how these
films behave under different temperatures. Key aspects include melting point, glass transition
temperature, thermal stability, and heat conductivity. This ensures the film's durability and
XRD 7 effectiveness in packaging, especially in maintaining its antimicrobial properties and protecting food
during processing, storage, and transport.
X-ray Diffraction (XRD) in antimicrobial packing films from biomaterials is used to analyze the film's
crystalline structure, detect changes due to added antimicrobial agents, assess nanoparticle
dispersion, and understand how these factors affect the film's mechanical and barrier properties.
8 MORPHOLOGY ANALYSIS
Morphology analysis in antimicrobial packaging films from biomaterials examines the surface and
structure to understand how they influence antimicrobial effectiveness, typically using microscopy
techniques like SEM or AFM.
Tasks Completed
The development of antimicrobial packing film involved the selection of appropriate biomaterials and antimicrobial agents. The
biomaterials were chosen based on their film-forming properties, biocompatibility, and potential for antimicrobial activity.
Extraction: 1
2kg of dried bagasse was taken and size was reduced till
it sieved through a mesh of 0.45mm)the fibrous materials
were removed leaving the raw material. The raw material 2 Alkali Treatment:
was dried at 60*C for 72 h and milled into powder. the raw material was then treated with NaOH 2 Litre 4%
[W/V])or 80gms of Pure NaOH
Bleaching: 3
The solution was bleached using an aqueous NaOCl
solution for 60 min with constant magnetic stirring at
80℃. The resulting residue, was cleaned with deionized Drying:
4
water until a neutral pH was reached and the product
The product was cleaned using deionized water and then
was dried at 60 ℃ for 24-36 hr.
left for drying at 55-60 degrees for 24-36 hr.
Film Formation
The biomaterial chosen for film formation was a biodegradable polymer, chosen for its biocompatibility and potential for antimicrobial activity. The film was
produced using a solution casting method, where the polymer was dissolved in a suitable solvent, cast onto a flat surface, and allowed to dry, forming a thin,
flexible film.
First, collected byproducts are After that, it is heated and treated Treated material is bleached with Product is dried and then size reduced.
crushed to the proper size. with alkali [NaOH]) in a magnetic NaOCl and washed until Neutral pH.
stirrer.
H2SO4 hydrolyzation continues in a Water is added to end hydrolyzation Material is dialyzed for five days It is dried and sonicated before
magnetic stirrer with low heat.
and obtain a colloidal solution. after centrifugation. being used in a film.
Anti-Microbial Agents
The antimicrobial agent used in this project was a natural essential oil
extracted from cinnamon bark. This essential oil is known for its broad-
spectrum antimicrobial activity, inhibiting the growth of various bacteria and
fungi.
Turmeric: Essential Oil:
This essential bioactive component, Essential Oils comprise a very
important group among the
which accounts for many of antimicrobial agents utilized in
turmeric's antibacterial effects, has active container. EO, like lemon peel,
been found to interfere with bacterial Zataria multiflora Boiss , orange
leaves, clove, thyme, and
cell membranes and prevent the cinnamon among others, oregano
growth of dangerous pathogens. has been incorporated into the
protein films with antimicrobial
activity against pathogens and food-
spoilage microorganisms
Conclusion
The development of biomaterial-based antimicrobial packaging
films represents a significant advancement in food processing
technology. The integration of natural antibacterial substances with
environmentally friendly and biodegradable materials presents a
viable solution to address the urgent problems of enhancing food
safety and reducing environmental impact. Our study
demonstrates how these coatings can stop microbes from growing,
increase shelf life, and be a useful replacement for traditional
petroleum-based polymers. This pioneering method not only
satisfies customer demands for ecological packaging but also
contributes in minimizing food deterioration and waste, so
encouraging a more sustainable food supply chain. Going forward,
successful integration within the food business will require more
research aimed at improving material properties and scalability.
THANK YOU
