Statistical Analysis
Statistical Analysis
x x
2 Legend:
S = standard deviation
s X = raw score
n
X = Mean
n = no. of scores
• A hen laid eight eggs. Each egg was weighed
and recorded as follows:
60 g, 56 g, 61 g, 68 g, 51 g, 53 g, 69 g, 54 g
Using the information from the above table, we can see that
x x = 320
2
n=8
In order to calculate the standard
deviation, we must use the following
formula:
x x
2
s
n
320
=
8
= 6.32 grams
1. Enter the data in column
2. Type the equal sign (=)
3. Type or Double click STDEVA
4. Highlight the data including the Mean and
type close parentheses
5. Click Enter
Common Inferential
Statistical Tools
How to add “Data Analysis”
in your Microsoft Excel for
Computer-Aided Instruction
1. Open Microsoft Excel, go to file and click
“Options”
2.Click Add-ins, Select Analysis ToolPak and
Click Go.
3. Select Analysis ToolPak and then click OK.
4. Click “Data” and find Data Analysis.
Tentative answer to a research problem
prior to experimentation
• Based your hypothesis from specific
objectives
Effect of Chicken Manure on the Growth of Mung Beans
Alternative Hypothesis
1. There is a significant difference among the different amount
of chicken manure as bio-fertilizer on the height and number
of leaves of mung beans.
2. There is a significant relationship between the height and
number of leaves of mung beans.
1. Formulate either null hypothesis or
alternative hypothesis or both
Research Objective
Is there a significant difference between
the glass with Silica microparticles and the
glass with Sodium carbonate in terms of
their weight and compressive strength?
Null Hypothesis
There is no significant difference between the
glass with Silica microparticles and the glass with
Sodium carbonate in terms of their weight and
compressive strength.
Alternative Hypothesis
There is significant difference between the
glass with Silica microparticles and the glass with
Sodium carbonate in terms of their weight and
compressive strength.
2. Set the level of significance (0.05; 0.01)
Level of Significance
• The probability of making an error in a
test
• A 5% significance level (0.05 α) means that
you can accept about 5 chances in 100 that
you would reject the null hypothesis. It
implies that you are 95% confident that
you have made the right decision
How about 1% significance level (0.01 α)?
• A 1% significance level means that
you can accept about 1 chance in 100
that you would reject the null
hypothesis. It implies that you are
99% confident that you have made the
right decision.
3. Compute the required statistical
test
• May use SPSS (Statistical Package
for the Social Sciences) or Microsoft
Excel ToolPak for the computation
• Commonly used inferential tools are
t-test for two dependent means, t-
test for two independent means
and One-way ANOVA
1. t-test
• Used to establish the
significance of the difference
between the means of the two
samples
t-test for two independent means
• Compares the mean score of two
different or independent groups
(treatment/control)
• The sample size is less than 30
There is no significant
difference between the glass with
Silica microparticles and the glass
with Sodium carbonate in terms of
their weight and compressive
strength.
Step 1:Enter the Data for A and B.
Step 2: Click tools on the Menu bar and select
Data Analysis
Step 3: The Data Analysis dialogue box
appears. Select t-test: Two Sample Assuming
Equal Variances and click OK.
Step 4: Enter the values of the Variable Range 1
and Variable Range 2. Enter the level of
significance of 0.05
Step 5: On the output option select output range
and click anywhere in the blank worksheet and
click OK.
Weight t-Test: Two-Sample Assuming Equal Variances
A B
Variable 1 Variable 2
Variable 1 Variable 2
Variable 1 Variable 2
Mean 6100 5833.333
Variance 10000 23333.33
Observations 3 3
Pooled Variance 16666.67
Hypothesized
Mean Difference 0
df 4
Tcalc t Stat 2.529822
P(T<=t) one-tail 0.032338
t Critical one-tail 2.131847
P(T<=t) two-tail 0.064677
Ttab t Critical two-tail 2.776445
Null Hypothesis
There is no significant difference
between the glass with Silica microparticles
and the glass with Sodium carbonate in
terms of their weight and compressive
strength.
Weight Tcalc (1.77) < Ttab (2.78)
Compressive Strength Tcalc (2.53) < Ttab (2.78)
Decision: Accept the null hypothesis
5. Summarize the result
Result shows that the glass with
Sodium sulfate microparticles is
comparable to the glass with Sodium
carbonate because the calculated t is lower
than the tabular t for the two
aforementioned parameters; therefore,
Sodium sulfate microparticles as a
component in glass making could be used
as a cheaper alternative to Sodium
carbonate.
t-test for two dependent means
• Compares the mean score of the
same group before (pretest) and
after (posttest) the treatment
There is no significant difference on the
total dissolved solids (TDS) of the
wastewater before and after the
introduction of water lettuce as
bioremediating agent.
TDS
Variable Variable
1 2
Mean 43.36667 25.8
Variance 1.163333 0.01
Observations 3 3
Pooled Variance 0.586667
Hypothesized
Mean Difference 0
df 4
DNA Concentration
Fcalc (7.83323) > Ftab (3.47805)
p= 5
Df = 10
p 2 3 4 5
TREATMENTS Difference Rp
T1 - T5
38.53 > 22.40 Significant Difference
90.13 - 51.60
T3 - T5
42.87 > 23.41 Significant Difference
94.47 - 51.60
T4 - T5
44.70 > 24.00 Significant Difference
96.30 - 51.60
T2 - T5
48.83 > 24.39 Significant Difference
100.43 - 51.60
T3 - T1 No Significant
4.34 < 22.40
94.47 - 90.13 Difference
T5 (1000 µL DNAzol) = 51.60 µg/µL
T1 (250 µL fish mucus) = 90.13 µg/µL p 2 3 4 5
T3 (750 µL of fish mucus) = 94.47 µg/µL rp 3.151 3.293 3.376 3.430
T4 (1000 µL fish mucus) = 96.30 µg/µL Rp 22.40 23.41 24.00 24.39
T2 (500 µL fish mucus) = 100.43 µg/µL
TREATMENTS Difference Rp
T4 - T1 No Significant
96.30 - 90.13 6.17 < 23.41 Difference
T2 - T1 No Significant
10.3 < 24.00
100.43 - 90.13 Difference
T4 - T3 No Significant
1.83 < 22.40
96.30 - 94.47 Difference
T2 - T3 No Significant
5.96 < 23.41
100.43 - 94.47 Difference
T2 - T4 No Significant
4.13 < 22.40
100.43 - 96.30 Difference
5. Summarize the result
Duncan’s Multiple Range Test (DMRT)
shows that the epidermal mucus from
Tilapia at different amount (250, 500, 750
and 1000 µL) yielded a significant higher
DNA concentration than the DNAzol
(positive control). Moreover, there is no
significant difference among the different
amount of epidermal mucus from O.
mossambicus in terms of the DNA
concentration.