CaptaVision+ Software Instruction Manual

for CaptaVision+ v2.4.1.10

ACCU-SCOPE, Inc. 73 Mall Drive, Commack, NY 11725

• 631-864-1000 (P) • [email protected] •
www.accu-scope.com

1
 Contents
General Introduction…………………………………… 3 Video Record …………………………………………25 Calibration……………………………………………49
1. Starting Interface……………………………………… 4 Video & Voice Recording………………………26 Export Calibrations…………………………………50
2. Windows……………………………………………………6 Delay Capture………………………………………27 Measure Layer………………………………………51
Status Bar ………………………………………………6 Trigger……………….…………………………………28 Metrics Flow…………………………………………52
Control Bar ……………………………………………7 Image Process….….…………………………………29 Graphics Property…………………………………53
Preview Window ……………………………………8 Save Settings……………………………………30 Manual Class Counting…………………………54
Data Bar …………………………………………………8 Light Frequency …………………………………31 Scale Property………………………………………55
Image Bar ………………………………………………9 Other Settings………………………………………31 Ruler Property………………………………………56
Short Cut Keys………………….……………………10 Auto Focus………………………………………31 Grid Property…………………………………………57
3. Capture…………………………………………………11 4. Image………………………………………………………32 Save Settings.…………………………………………58
Resolution……………………………………………11 Control Interface……………………………………32 Fluorescence Intensity……………..………….59
Binning………………………………………………11 Image Adjust…………………………………………33 Cursor Property……………………………………60
Exposure Control……………………………………11 Image Dye……………………………………………. 34 6. Report………………………………………………………61
Bit of Depth……………………………………………12 Fluorescence…………………………………………35 Template Report…….......………………………61
White Balance………………………………………13 Advanced Computational Imaging…………36 Report………………………………………………….62
Histogram………………………………………………14 Binarization…………………………………………37 7. Display………………………………………………………63
Image Adjust………………………………………15 Histogram…………………………………………….. 38 8. Config…………….…………………………………………64
Background Correction………..………………..16 Smooth…………………………………………………39 Capture / Image / Measure…………..……..64
Temperature Control….…………………………17 Filter/Extract/Inverse Color……………………40 Jpeg……………………………………………………..65
File Save…………………………………………………18 Deconvolution…………………….…………………41 9. Info……………………………………………………………66
ROI………………………………………………………19 Automatic Counting……..…………….…………42 Preferences……………………………………………66
Cover……………………………………………………20 Automatic Counting Property…….…………45 Help………………………………………………………66
Imaging Stitching(Live)…………………………21 5. Measure……………………………………………………46 About……………………………………………………67
EDF(Live)……………………………………..……….22 Control Interface……………………………………46 10. Limited Warranty…………………………..………68
Dark Field/Fluorescence Imaging…………23 Measure Tool…………………………………………46

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 General Introduction

CaptaVision+ is a powerful software that integrates the micro-imaging camera control, image calculation and
management, image processing into a logical workflow for acquisition, processing, measuring and counting to give
› Contents scientists and researchers a more intuitive operating experience.
› General Introduction
CaptaVision+ drives and controls our Excelis portfolio of cameras, to give you the best performance in your microscopy
› Starting Interface
imaging applications. Through its user-friendly and logical design, CaptaVision+ helps users maximize the potential of
› Windows their microscope and camera system for their research, observation, documentation, measurement and reporting tasks.

› Capture
CaptaVision+ allows users to customize their desktop within the application according to their application and need.
› Image Users can turn features on or off, and arrange the menus to follow your workflow. With such control, users are assured
› Measure of completing their imaging work with more efficiency and efficacy, generating results faster and with greater confidence
than ever before.
› Report

› Display Thanks to its powerful real-time calculating engine, CaptaVision+ achieves superior quality images with less effort by the
user. The real-time stitching feature allows the user to capture a super wide Field of View (an entire slide if desired)
› Config
simply by translating a specimen on the mechanical stage of a microscope. In about 1 second, the real-time Extended
› Info Depth of Focus (“EDF”) feature can rapidly assemble in-focus features of a specimen as the focal plane passes through it,
resulting in a 2-dimensional image containing all of the detail of the 3-dimensional sample.
› Warranty

CaptaVision+ was developed from the user’s perspective, assuring the best operating procedures through the
implementation of its all-new camera operating workflow with modular menus for efficient image acquisition → Image
processing and editing → measurement and counting → reporting of findings. In conjunction with the latest image
processing algorithms, the workflow saves time from the moment the imaging process begins to the delivery of a report
at the end.

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 Starting Interface

› Contents

› General Introduction When starting CaptaVision+ for the first time, a biological or industrial
application option box will display. Select the desired application type to finish launching
› Starting Interface
the software. CaptaVision+ will automatically optimize parameter settings based on your
› Windows choice. This setting will be remembered by CaptaVision+ the next time you launch the
software.
› Capture • [ Biological ]. The default is to use automatic white balance with gamma value 2.10 and
the mode of exposure to the right.
› Image
• [ Industrial ]. The default color temperature value is set to 6500K. CaptaVision+ is set to
› Measure use area white balance with a gamma value of 1.80 and middle exposure mode.

› Report You may also change the application type preference through [Information] > [Options] >
[Microscope] in the upper right part of the menu bar.
› Display

› Config

› Info

› Warranty

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 Starting Interface

› Contents

› General Introduction

› Starting Interface

› Windows

› Capture

› Image

› Measure CaptaVision +
› Report

› Display

› Config

› Info

› Warranty
Note:
1) The CaptaVision+ software launches very quickly, typically within 10 seconds. It may take longer for particular cameras e.g., MPX-20RC.
2) If no camera is detected or an unsupported camera is connected when CaptaVision+ launches, the software will only support image
processing, measurement operations and report operations.

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 Windows

› Contents
Main Interface
The CaptaVision+ software interface consists of 5 main areas:
› General Introduction
① Status Bar
› Starting Interface
There are eight main modules in the status bar: Capture / Image /
› Windows Measure / Report / Camera List / Display / Config / Info. Click on the
module tab and the software will switch to the related interface.
› Capture

› Image CaptaVision+ v2.3 supports multiple camera connections and hot

swapping of cameras. For USB3.0 cameras, please use
› Measure
computer's USB3.0 port for the hot swap, and do not unplug or
› Report plug in the camera when the camera list is refreshed.
In the camera list, the recognized camera model is displayed. Click
› Display the camera name to switch to that camera.
When the current camera is removed, it will automatically switch to
› Config
another camera, or display no camera.
› Info
① Status Bar ② Control Bar ③ Preview Window ④Data Bar ⑤Image Bar
Note: Hot swap is not supported in the MacOS version of
› Warranty CaptaVision+.

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 Windows

② Control Bar
› Contents

› General Introduction To display the available functions and controls within a module, click the
button to expand the function. Click the button to collapse the
› Starting Interface
display of the functions.
› Windows

› Capture

› Image

› Measure

› Report

› Display

› Config

› Info

› Warranty

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 Windows

› Contents ③ Preview Window

› General Introduction

› Starting Interface

› Windows

› Capture

› Image

› Measure

› Report

› Display

› Config
To display live and captured images.
④ Data Bar
› Info With the cursor placed over the image, use the wheel of the mouse to zoom in
and out of the image, show the magnified area around the cursor in the middle
› Warranty of the screen.
Hold down the left button / right button / scroll wheel of mouse to drag the
image display area. Displays the measurement and statistics tables. This is where
Click the control button at the edge of the window: , , , measurements, calibrations and counts will be collected and
to show or hide the corresponding operating bar. available to apply (e.g., calibrations) or export.
Click the button to save the currently selected picture as another format The measurement table supports the export of custom templates.
(see the “Save image” dialog figure in upper right). The software supports four For specific instructions, please refer to the Report chapter.
image formats for saving or saving as: [JPG] [TIF] [PNG] [DICOM]*.
*DICOM format is not available in the Macintosh version of CaptaVision+.

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 Windows

⑤ Image Bar
› Contents
a) Click the button to locate the saving path of the file, · Click the button to add the current saving path
› General Introduction The Image Bar displays select the desired directory from which the image will be to the favorites folder for fast access the next time.
thumbnails of all captured opened, and the interface changes to the following view. · Click the button to return to the upper directory.
› Starting Interface images and videos from all
saving paths. Click on any
› Windows · The button in the upper right
thumbnail and the interface
automatically switches to the corner of the dialog box allows
› Capture
[Imaging] window for image you to choose the thumbnail
› Image processing. display size.

› Measure

› Report

› Display
· Select the files-saving path on the left side. Click the
› Config button to close the window.
b) Right click on an image or on the blank
› Info
area of the interface to display the
operation menu, and choose from the
› Warranty
operations to perform: “Select All”,
“Deselect All”, “Open”, “New Folder”,
“Copy”, Paste”, “Delete” and “Rename”. You can also use
Ctrl+c and Ctrl+v shortcut keys to copy and paste images. ;
Select the files-saving path on the left side. Click the
button to close the window.
· The saving path and all the images under this path will
display on the right side of the window.

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 Windows

› Contents

› General Introduction b) Right click on an image to choose from

operations such as “Rename”, “Close”,
› Starting Interface
“Close all”, “Delete” and “Compare”.

› Windows

› Capture
After choosing “Compare”, the user can choose “Dynamic” or
› Image “Static”.
Dynamic compares a live preview image with a saved image. With a
› Measure
live preview image active, place the cursor over a saved image in the
› Report picture bar and right-click, then choose [Contrast]. The live preview
image displays on the left side, and the saved image on the right.
› Display Saved images can be changed any time.
⑥ Shortcut Keys
Static compares two saved images. Place the cursor over a saved For convenience, CaptaVision+ provides the following shortcut key functions:
› Config
image in the picture bar, right-click the mouse and choose [Contrast]. Function Key Remarks
› Info Repeat with a second saved image. The first selected image will
appear on the left. To replace an image, click on it in the viewing Capture F10 Take and automatically save image
› Warranty window, then move the cursor to the picture bar to choose another
Record video F11 Press to start recording; press again to stop
image. recording
Click in the upper right corner to exit Contrast viewing.
Close all F9 Closes all image thumbnails in picture bar
The Contrast view can also be saved.
Save image as F8 Specify image format or save location

Pause F7 Pause/Resume the live view. When the

preview is paused, a pause state prompt is
displayed as shown here:

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 Capture

Capture
› Contents
➢ Click the camera button to capture an image of the live view. Also supports continuous
› General Introduction click.
Resolution
› Starting Interface
Resolution Setting
› Windows ➢ Resolution: select the resolution of the preview image and captured image. A lower
preview resolution will typically provide a better image when moving the sample (faster
› Capture camera response).
Note:
› Image
• High Speed binning resolution of monochrome cooled camera can increase the frame rate:
• Compressed resolution of HDMI series cameras will increase frame rate and increases CPU
› Measure usage.

› Report Binning
If supported by your camera, Binning mode can improve the sensitivity of the image
› Display
particularly in low light applications. The larger the value, the greater the sensitivity.
› Config Binning works by adding signal in adjacent pixels and considering it as one pixel. 1x1 is the
default setting (1 pixel by 1 pixel).
› Info Note: This function performs sum binning based on the original data, the amount of data does
not change, so the frame rate does not change.
› Warranty
Exposure Control
Set the exposure time of camera and calculate the real-time frame per second (fps) will be
displayed.
➢ Target Value: Adjusting the target value changes the automatic exposure brightness of
the image. Target value range for MPX series is 10~245; HDMI (HD, HDS, 4K) series is 0-15.

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 Capture

Exposure Control (continued)

› Contents
Auto Exposure: Check the box before [Auto Exposure] and the software automatically
› General Introduction adjusts the exposure time to achieve the appropriate brightness level. The automatic
exposure time range is 300µs~350ms. Exposure Time and Gain are not available to change
› Starting Interface
in Auto Exposure mode.
› Windows Area Exposure: Check the [Area Exposure], the software automatically adjusts the exposure
time according to the image brightness in the area.
› Capture Manual Exposure: Uncheck the box next to [Auto Exposure] and the software enters
[Manual Exposure] mode. The user can manually enter exposure time into the boxes, then
› Image
click [OK] button to apply, or manually adjust the exposure time with the slider. The
› Measure manual exposure time range is 130µs~15s.
➢ Gain: User can select the most suitable gain setting depending on application and needs
› Report
for generating a good image preview. Higher gain brightens an image but may also
› Display produce increased noise.
➢ Default: Click the [default] button to restore this module's parameters to the factory
› Config default. The default setting is [auto exposure] .

› Info
Bit Of Depth (Bit Depth)
› Warranty
ONLY FOR MONOCHROME CAMERA WITH COOLING
Where supported by the camera, the user can choose standard (8 bit) or high (16 bit)
bit depth. Bit depth is the number of levels in a channel and is noted as the
exponent to 2 (i.e. 2n). 8 bit is 28 = 256 levels. 16 bit is 216 = 65,536 levels. Bit depth
describes how many levels can be distinguished between black (no signal) and white
(maximum signal or saturation).
Default bit depths: Monochrome cooling camera: 16 bits; USB 3.0 color camera: 8
bits; USB 2.0 camera: 8 bits (16 bit not supported)

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 Capture

› Contents
White Balance

› General Introduction White Balance provides for more consistent images, accommodating for changes in
light composition and its impact on the sample.
› Starting Interface
➢ White balance: By adjusting the ratio of the three individual components of red,
› Windows
green and blue, the camera can reflect the true image color under various
› Capture illuminating conditions. The default setting of the white balance of the camera is
Auto-white balance (enabled when [Lock WhiteBalance] is unchecked). To
› Image manually set white balance, uncheck [Lock White Balance], move the sample out of

› Measure the light path or place a white or neutral gray paper under the camera, then
recheck [Lock White Balance] to lock the current white balance setting.
› Report ➢Area white balance: In Biology mode and when [Area White Balance] is selected, Color temperature(CCT): The current close color temperature can
a region for measuring white balance opens on the preview image. be achieved by adjusting the three gains which are Red, Blue and
› Display Green (as above). It can also be manually adjusted and matched to
In Industry mode, an area white balance box is displayed on the preview image.
The size of area white balance box is adjustable. Under a stable lighting approximate the color temperature of the illuminating environment.
› Config
environment, drag the area white balance box to any white part of the image, Manually setting the white balance is more accurate at achieving
› Info adjust its size, and check [Lock White Balance] to lock the current white balance the correct color temperature. Color temperature setting range is
setting. 2000K to 15000K.
› Warranty ➢ Gray: Check this box to convert a color image to a monochrome image. ➢ Default: Click the [Default] button to restore this module's
➢ Red, Green, and Blue(Gain): Manually adjust the gain values of the red, green, parameters to the factory default. The default setting of white
and blue channels for suitable white balance effect, adjusting range is 0~683 balance is [Auto-white balance].

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 Capture

Histogram

› Contents Color level adjustment can lead to more realistic images for observation and analysis.
Red (R), green (G) and blue (B) color levels can be adjusted in each channel, and the
› General Introduction associated pixel values distributed accordingly. Adjust the color level (gradation) to
increase or decrease the range of the highlight area in the image. Alternatively, the
› Starting Interface
color components of the individual RGB channels can be adjusted separately.
› Windows When used with white balance and a neutral target, each color channel of the
histogram will overlap as illustrated in the figure to the right. The values for Max and
› Capture Gamma will vary by camera series.

› Image ➢ Manual Color Level: Manually adjust the image's dark tone (left gradation), the
gamma and highlight brightness level (right gradation) on the histogram to regulate
› Measure the image's tones, such as the contrast, shading and image layers, to obtain the Example histogram of an empty field with proper white
balancing. All color channels overlap exactly.
desired balance of the whole image.
› Report ➢ Auto Color Level: Check [Auto Min] and [Auto Max] to automatically adjust the
brightest and darkest pixels in each channel as white and black, and then re-distribute
› Display
the pixel values in proportion.
› Config ➢ Gamma: Non-linear adjustment of the median of the color level, often used to
“stretch” darker areas in the image to see more detail. Setting range is 0.64 to 2.55
› Info ➢ Line or Logarithm: The histogram supports Linear (Line) and logarithmic display.
➢ Default: Click [Default] button to restore the module's parameters to the factory
› Warranty default setting. The default of color level adjusting is manual, and the default gamma
value is 2.10.

Note: a) The composing and displaying of the histogram curve is the result of the
software’s running real-time data statistics, so some the resources of the software
will be used. When this module active, the camera frame rate may be affected and
drop slightly. When the module is not used (set to Default), the data statistics are
turned off and the frame rate of the camera can reach the maximum based on other
camera settings. b) After cancelling the automatic color level adjusting, the level value
Example histogram of a sample with color. Note the multiple
will be the default (0 and max). peaks as compared to the empty field example above.

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 Capture

› Contents
Image Adjust

› General Introduction The user may perform real-time dynamic adjustment of images to achieve the
desired image effect. Parameter ranges may be different by camera series.
› Starting Interface
➢ Hue: Adjusts the shade of the color, adjusting range from 0 to 360.
› Windows
➢ Saturation: Adjusts the intensity of the color, the higher the setting, the more
› Capture vivid the color. A setting of “0” is essentially monochromatic. Setting range is 0~255.
➢ Light: The brightness and darkness of the image, setting range is 0~255
› Image ➢ Contrast: The difference in brightness level between the brightest white and the
darkest black in the light and dark areas of an image, setting range is 0~63. Default
› Measure
is 33.
› Report ➢ Sharpness: Improves clarity of feature edges in the image.
➢ Permeability: The sharpness effect of the image, setting range is 0~48 for MPX
› Display Image Adjust menu for MPX-20RC camera.
series cameras. Default is 16.
› Config ➢ DPC: Reduce bad pixels on camera.
➢ Black level: ONLY FOR MONOCHROME CAMERA WITH COOLING. Adjust the gray
› Info value of a dark background, range is 0-255. Default is 12.
➢ 3D Noise reduction: Automatically averages adjacent frames of images to filter
› Warranty out non-overlapping information (“noise”), thereby producing a cleaner image.
Setting range is 0-5 frames for MPX-20RC. Default is 3.
➢ Default: Click the [Default] button to restore this module's parameters to the
factory default ones.
The factory default values of some parameters (settings) for image capturing
(acquisition) are as followed: Hue:180/ Contrast:33/ Saturation:64/ Brightness:64/
Permeability:16/ [Image Enhancement Save] on uncheck/ Image Enhancement:1/ Image Adjust menu for Excelis HD series
Noise reduction:1 cameras.

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 Capture
Image Adjust: Background Correction
➢ Flat Field Calibration: In microscopy applications, live and captured images may
› Contents contain uneven illumination, shading, vignetting, color patches or dirty spots due to
microscope illumination, microscope alignment, optical path systems and alignment
› General Introduction or dirt in the optical system (objectives, camera couplers, camera window or sensor,
internal lenses, etc.). Flat field correction compensates for these types of image
› Starting Interface defects in real-time through a reduction of repeatable and predictable artifacts to
deliver an image with a more uniform, smoother and realistic background.
› Windows
Operation:
› Capture a) Click [Flat Field Calibration Wizard] to initiate the process. Move the specimen out
of the camera’s field of view (FOV) to a blank background, as shown in the right
› Image
figure (1). It is recommended to move the sample/slide completely out of the FOV.
› Measure See Note c) below for reference to reflected light applications;
b) Click [Next] then move the first background to another new blank background,
› Report click [OK] to apply the Flat Field Calibration function, as shown in the right figure(2);
c) Select [ uncheck] to exit the flat field correction mode. If you need to apply it
› Display again, re-check it, no need for repeating the wizard procedures again.
➢ Default: Click the [Default] button to restore this module's parameters to the
› Config
factory default ones.
› Info
Note:
(1)
a) The Flat Field Calibration requires manual setting of the exposure time, so that the
› Warranty
image brightness will not overflow up or down, and all the pixel values are range
from 64DN to 254DN (i.e., the background should not be white, rather slightly gray).
b) The brightness of the two backgrounds being used for correction should be similar,
and some different spots on the two backgrounds is acceptable. (b)
c) Plastic, ceramic or professional white balance paper are recommended as the
standard samples for flat field correction in reflected light applications.
d) For optimal results, Flat Field Correction requires backgrounds with uniform or
predictable illumination. NOTE: Repeat Flat Field Correction for every
lens/objective/magnification change. (2)

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 Capture

Temperature Control
ONLY FOR MONOCHROME CAMERA WITH COOLING
› Contents
CaptaVision+ supports the temperature adjustment of cameras with cooling;
› General Introduction optimal noise reduction can be achieved by reducing the working temperature of
the camera sensor.
› Starting Interface ➢ Current: Displays the current temperature of the camera sensor.
➢ Cooling: Offers three options – Normal Temperature, 0°, Low Temperature. The
› Windows
user can choose a Cooling setting that best fits the imaging experiment.
› Capture ➢ Fan Speed: Control the fan speed to increase/decrease cooling and to reduce
noise from the fan. Default setting is High and is adjustable to medium and low
› Image speed. NOTE: Slower fan speeds reduce noise but provide less effective cooling. This
feature is only for Monochrome Cameras with cooling.
› Measure ➢ Default: Restores current settings to factory default settings – Low Temperature
and High fan speed.
› Report

› Display Note: When the external environment temperature is too high, a high temperature
warning prompt message may appear, and the indicator light on the camera will
› Config flash red. This feature is only for Monochrome Cameras with Cooling.

› Info

› Warranty

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 Capture

File Save
Capture the currently needed data from the real-time video data stream and record
› Contents it into image format for later development and analysis.
Click the button to capture a preview image and display the File
› General Introduction
Save dialog.
› Starting Interface
➢ Use Dialog: After clicking Capture, CaptaVision+ opens a Windows File Explorer or
Finder dialog window for naming and saving the image file.
› Windows
➢ Use File name: The name of the file to be saved is "TS" by default and can be
› Capture readily edited by the user.
The software supports file name suffix format of
› Image "custom + time-stamp". There are four formats
of time-stamp naming available, and numerical
› Measure suffix augmentation (nnnn). ➢ Path: The destination path for saving images. User can click
Note: The file name cannot contain special characters \/:*?”<>|. Using these characters
› Report the [Browse] button to change the saving path. The default
will generate an error message as below:
saving path is C:/Users/Administrator/Desktop/Image. The path
› Display cannot contain special characters \/:*?"<>|, or an error message
will be displayed.
› Config ➢ Format: Images may be saved as JPG\TIF\PNG\DICOM files. The default format is
➢ Saved with time format: The capture time will be displayed
TIF. The formats may be checked individually or in multiples. Captured images
and burned into the lower right corner of the image.
› Info saved in multiple formats will be displayed together.
➢ 3D Denoise Save: Obtain low-noise images by acquiring and
1) JPG: an information-losing and compressed image saving format, its image size is
comparing image data of several consecutive frames, and then
› Warranty small, but the image quality is degraded compared with the original.
filtering out non-overlapping information. The number of frames
2) TIF: a Lossless image saving format, saves all the data transmitted from the
can be customized. The range is 1~99, the default is 10.
camera to your storage device without losing data. TIF format is recommended
Note:
when high image quality is required.
• To get 3D denoise image, so it will take longer time than
3) PNG: Portable Network Graphics is a lossless but compressed bit-image format
taking a general image.
that uses a compression algorithm derived from LZ77 with a high compressing ratio
• 3D Denoise Save is only suitable for capturing static scenes;
and a small file size.
• 3D noise reduction has no effect on video recording.
4) DICOM: Digital Imaging and Communication Of Medical, an international standard
format for medical images and related information. It defines a medical image
format that can be used for data exchanging and meet the requirements of clinical
practices and applications. Not available on Macintosh versions of CaptaVision+.

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 Capture

› Contents ROI
ROI (Region of interest) allow the user to define a window area of interest within the
› General Introduction
effective and sensitive detecting area of the camera sensor. Only image information
› Starting Interface within this defined window will be read out as the image view and, as such, the
image is smaller than capturing an image with the full camera sensor. A smaller ROI
› Windows
area reduces the amount of information and task of image transfer and computer

› Capture processing resulting in a faster frame rate of the camera.

› Image Regions of interest can be defined using two methods: draw using a computer
mouse and specify the X and Y pixel locations (starting point with height and width).
› Measure
➢ Select ROI: Using a computer mouse, check the box next to “Selecting regions of
› Report
interest(ROI)”, then move the cursor to the preview. Click and drag to define the
› Display window area to use as the ROI -- the window area will display the coordinate values
and resolution of the current selection. Click on the [√] below the cursor to apply the
› Config ROI settings.

› Info
➢ Select Area and Coordinate of ROI：The user can manually enter the starting
› Warranty point coordinate values and the resolution size (height and width) to define the
exact ROI area. Enter the actual point offset position of the rectangular area as well
as the width and height, then click [OK] to apply the ROI settings.

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 Capture

› Contents Mask (previously called Cover)

Almost opposite to ROI, the Mask feature is useful to block an area of the image
› General Introduction
being viewed to allow the user to concentrate on the area of interest without
› Starting Interface distraction. Mask does not reduce the area of the camera sensor doing the imaging
nor the amount of data being transferred and, therefore, does not provide any
› Windows
increase in frame rate or imaging speed.

› Capture
Masked areas can be defined using two methods: draw using a computer mouse and
› Image specify the X and Y pixel locations (starting point with height and width).

› Measure
➢ Select Mask: Using a computer mouse, check the box next to “Select Mask”, then
› Report move the cursor to the preview. Click and drag to define the window area to use as
the Mask -- the window area will display the coordinate values and resolution of the
› Display current selection. Click on the [√] below the cursor to apply the Maksk settings.

› Config
➢ Set Area and Coordinate of Mask: The user can manually enter the starting point
› Info coordinate values and the resolution size (height and width) to define the exact
Mask area. Enter the actual point offset position of the rectangular area as well as
› Warranty the width and height, then click [OK] to apply the Mask settings.

➢ Transparency: Manually select the opacity of the unselected area.

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 Capture

Imaging Stitching(Live)*
› Contents
Real-time image stitching acquires individual images with overlapping and adjoining
› General Introduction positions on the specimen or sample and combines them into a stitched image to present a
larger view or the entire specimen at higher resolution than could be obtained with the
› Starting Interface microscope set up.

➢ Stitching Speed: Two options: High Speed (default) and High Quality.
› Windows
➢ Background Color: The default background color of the unused area on the stitched-to-
composed image is black. If desired, click on to choose another color for the
› Capture background. This color background is visible in the final stitched image.
➢ Start Stitching:
› Image 1. Click [Start Stitching] and a reminder prompt figure (1) is displayed; The cache
memory of the computer will be used to save image data during the stitching
› Measure procedure. To maximize performance, close all applications not in use. Figure (2)
shows the current field (left) and assembled stitched image during the stitching (1)
› Report process.
2. Move the specimen to another new position (keeping about 25% overlap with the
› Display former position) and then pause, the navigation frame in the stitching window will
NOTE:
turn from yellow to green (figure (3) indicating that the new position is being Image Stitching
› Config stitched to the previous. (2) (Live) is not
3. Repeat the process until the stitched area meets your expectation. If the navigation
› Info frame turns red as shown in the right figure (4), the present position is too far from a
supported by
previous position to be stitched – to correct this, move the specimen position 32-bit operating
› Warranty towards a previously stitched area, the navigation frame will change to yellow then systems.
green and stitching will proceed. (3)
4. Click [Stop Stitching] to end the stitching, and a stitched composite image will be NOTE:
generated in the image gallery. Image Stitching
Note: a) Dynamic stitching is not supported by 32-bit operating systems. is not available
b) It is recommended to conduct a white balance correction and flat field correction before
(4)
on Excelis 4K
starting the stitching to ensure the best quality images.
c) Ensure exposure time is 50ms or lower for best performance.
when using
d) Stitched images are very large in size and occupy considerable memory resources of the Ethernet mode.
computer. It is recommended to use Image Stitching with a computer with sufficient
memory volume. A 64-bit computer is required. c) When the stitching process uses 70% of
the computer memory volume, the stitching module will stop working automatically.

*not available on Excelis 4K when using Ethernet mode

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 Capture

› Contents EDF(Live)*

› General Introduction EDF (Extended Depth of Focus) merges in-focus images across multiple focus planes to
generate a 2-dimensional images with everything in focus. EDF is ideally suited for “thicker”
› Starting Interface specimens or samples (e.g., an insect as opposed to a thin tissue specimen). The EDF image
allows for easy observation of sample detail all at once.
› Windows
NOTE: EDF is not suitable for use with Greenough-style stereo microscopes as the EDF
› Capture
function will produce a “smeared” image due to the optical design of the microscope.
NOTE: Extended Depth of Focus (EDF) is not
› Image When using EDF with Galilean-style (a.k.a. Common Main Objective, CMO or Parallel Light
Path) stereo microscopes, the objective must be moved to an on-axis position.
supported by 32-bit operating systems.
› Measure NOTE: EDF is not available on the Excelis 4K
➢ Quality: High quality setting acquires and merges images at a slower speed but generates when using Ethernet mode.
› Report
higher image quality in the final EDF image.

› Display
1. Click the [Start EDF ] button to run.
› Config 2. Continuously turn the fine focus knob of the microscope to focus through the specimen,
the software automatically merges the obtained focus plane images and shows out the
› Info current result in a live preview.
3. Click the [Stop EDF] button to end the stacking and merging process, a new merged
› Warranty image including all the depth focusing information will be generated in the image gallery.

*not available on Excelis 4K when using Ethernet mode

Left: EDF image. Right: As seen

through the microscope.

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 Capture

› Contents
Realtime Dye (Fluorescence Imaging)

› General Introduction This mode is usually used when acquiring fluorescence images with a monochrome
camera to automatically add color to the monochrome image. The “dyed” image can
› Starting Interface later be used to synthesize a composite fluorescence image overlaying multiple dyes.

› Windows
Select the desired color (representative of a selection of dyes), click [Apply] to apply
› Capture selected color to pictures, and click [Cancel] to cancel currently applied color. The
false-colored image can be saved and used for creating polychromatic/multi-channel
› Image fluorescent image at a later time.

› Measure
➢ Current: This window displays the currently available colors that can be selected by
› Report the user, there are the seven commonly used colors. Click to display the full color
palette for a much broader selection of color choices. After selecting the color, click
› Display
[OK] to accept the color.
› Config You can click [Add to New Dyes] to add a color to your pallet for later use. Simply select
your color, then click the [Add to New Dye] button. Your new color will appear in the
› Info New Dye dropdown list.
➢ Add to New Dyes: Adds selected colors on the palette into the New Dye list.
› Warranty ➢ Dye Type: Displays list of common dyes, each associated with a color as observed in
the microscope.
➢ New Dye: Displays user-defined dye colors.
➢ Delete: Deletes the selected user-defined dye color.
➢ Apply: Applies the selected dye color to the image.
➢ Cancel: Cancels the application of certain dyes added through custom mode.

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 Capture

› Contents
Dark Field/Fluorescence Imaging (continued)

› General Introduction Select the desired color (representative of a selection of dyes), click [Apply] to apply
selected color to pictures, and click [Cancel] to cancel currently applied color. The
› Starting Interface false-colored image can be saved and used for creating polychromatic/multi-channel
fluorescent image at a later time.
› Windows
➢ Current: This window displays the currently available colors that can be selected by
› Capture the user, there are the seven commonly colors. Click to display the full color
palette for a much broader selection of color choices. After selecting the color, click
› Image [OK] to accept the color.
You can click [Add to Custom Colors] to add a color to your pallet for later use. Simple
› Measure
set or select a color and click the [Add to Custom Colors] button.
› Report ➢ Add to New Dyes: To add selected colors on the palette into the new dyes.
➢ Cancel: To cancel a certain kind of dyes added through custom mode.
› Display
➢ Dye Type: The user may be able to quickly select a color based on the fluorochrome
› Config used in the specimen staining process and apply that color to the monochrome image.

› Info

› Warranty

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 Capture
Video Record & Voice Record

› Contents Click on [Video Record], save the image data in a video format for play back to observe
the sample/specimen movement or change over time. Check [Voice Recording] to
› General Introduction record audio with the video (requires microphone).

› Starting Interface ➢ Encoder: The software provides two compressing formats: [Full frame (No
compression)] and [MPEG-4]. MPEG-4 videos are typically much smaller files than
› Windows without compression, and the user should choose the format that best suits his need.
➢ Auto Stop: Check the Auto Stop box to activate options for capturing a designated
› Capture number of frames or for a specific time duration.
➢ Total Frame: Capture images according to how many frames are desired to be
› Image
captured, setting range is 1~9999 frames. Camera will operate at the frame rate shown
› Measure in the Exposure Control menu.
➢ Total Time(s): The length of time of video capture at the frame rate shown in the
› Report Exposure Control menu, setting range is 1~9999 seconds.
➢ Delay time: Assign a delay in capturing images, then capture per total frames or total
› Display time. Choose minute, second and millisecond. The delay time range is 1 ms to 120 min.
➢ Playback Rate: Records video according to the designated playback frame rate.
› Config ➢ Save to RAM: The image data is temporarily saved in the
➢ Video Format: AVI\MP4\WMA are supported, the default is AVI format. For smaller
computer’s RAM, then transferred to the hard drive after image
videos, choose MP4.
› Info capture is complete. Select Save to RAM and enable the RAM to
➢ Voice Recording: Check this box to record audio with the video. Audio will be
save images. The software calculates and displays the maximum
recorded after clicking the [Video Record] button.
› Warranty number of images that can be saved to RAM based on available
➢ Save to Hard Disk: The video file is saved directly to the hard disk. Since the
capacity. This mode allows a high transmission speed of the
computer takes time to write files to the hard drive, the transmission of data from the
images, but is limited by the available RAM capacity, therefore it is
camera to the hard drive is reduced. This mode is not recommended for capturing
not suitable for long video recording or high volumes of captured
video at fast frame rates (quickly changing scenes or backgrounds), but it is suitable for
images.
long capture periods.
➢ Video Record: Click this button to initiate video capture
according to the settings above.

➢ Default: Click the [Default] button to restore the module's

parameters to the factory default. The default is the compressed
mode with full resolution frame, 10 total frames, and a 10 second
capture time, with image data saved to the local hard drive.

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 Capture
Voice Recording

› Contents Found in the Video Record menu, check the box next to click the check box next to
[Voice Recording] to activate this feature. When recording a video, an audio track will
› General Introduction be saved with the video track.

› Starting Interface NOTE: The user must have a microphone on the computer.

NOTE: It is highly recommended to save the video in MP4 format or WMA format.
› Windows
AVI videos with audio are very large (10 second video = 1.5GB).
› Capture NOTE: Recordings with audio will only be saved to the hard disk.

› Image

› Measure

› Report

› Display

› Config

› Info

› Warranty

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 Capture

Delay Capture
› Contents
Also known as time lapse, Delay Capture allows the user to specify the number of
› General Introduction
frames to capture and the time period between frames. The captured images will
› Starting Interface be saved in a video format.

› Windows ➢ Total Time: Capture images according to the time set (in seconds). Default is 1
second, the setting range is 1-9999s.
› Capture ➢Total Frame: Capture images according to the number of desired frames, system
default is 10 frames, setting range is 1~9999 frames.
› Image
➢ Playback Rate: Set the frame rate at which the video will play back. The default is
› Measure 25 with a range of 1-10000 frames per second.
➢ Interval Time: Set the time between images. The default interval time is 0
› Report minutes. Three time units may be selected: Minutes, seconds and milliseconds. The
available range is 0ms-120min. The minimum value is zero meaning images will be
› Display
captured as fast as possible depending on the camera, processing speed and
› Config memory of the computer.
➢ Delay time: Applies a delay before acquiring the first image of the series. Set the Note: There is a quantitative relationship between the total
› Info time (delay) before the first image will be captured. Time units: minutes, seconds time, the total number of frames, and the playback frame rate.
and milliseconds; the range is 1 millisecond to 120 minutes. Total time is calculated as below, then rounded down.
› Warranty
➢ Video Format: Choose a file format for the video. AVI\MP4\WAM are supported.
The default format is AVI. 𝑡𝑜𝑡𝑎𝑙 𝑛𝑢𝑚𝑏𝑒𝑟 𝑜𝑓 𝑓𝑟𝑎𝑚𝑒𝑠
➢ Capture Frame: Capture and save frames/images according to the settings in Total time =
𝑝𝑙𝑎𝑦𝑏𝑎𝑐𝑘 𝑓𝑟𝑎𝑚𝑒 𝑟𝑎𝑡𝑒
entered in the Delay Capture dialog. Click [Stop] to terminate the capture process
early, before all of the frames have been captured.
➢ Capture as Video: Capture multiple frames/images according to the set
parameters and save them directly as a movie (AVI file is the default). Click [Stop] to
terminate the capture process before its conclusion.

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 Capture

Trigger
› Contents
ONLY FOR MONOCHROME CAMERA WITH COOLING
› General Introduction
Two output modes are available: Frame mode and Flow (stream) mode.
› Starting Interface ➢ Frame mode: The camera is in external trigger mode and outputs images by
triggering the frame capture. This can be done with a Hardware trigger or Software
› Windows trigger.
➢ Flow mode: real-time preview mode. Data flow is the output mode. Embed image
› Capture
data in the stream. The image is output circularly like flowing water.
› Image ➢ Hardware setting:
▪ “Off” mode: Indicates that the hardware trigger mode is off at this time, and
› Measure the camera is producing a live image.
When the “On” mode is selected, the camera switches to the trigger waiting mode,
› Report
and imaging is paused. Only when the trigger signal is received will the camera
› Display capture an image.
▪ “On” mode: Turn on the hardware trigger and enter the standard trigger
› Config
mode. There are several configuration modules (Exposure and Edge):
› Info ➢ Exposure: Note:
▪ Time: The exposure time is set by the software. 1) Changing between Hardware “On” or “Off”, the settings for
› Warranty ▪ Width: Indicates exposure time is set by input level width. Exposure, Edge and Exposure Delay take effect immediately.
➢ Edge: 2) When you close the software, the software will reopen the
▪ Rising edge: Indicates that the trigger signal is valid for rising edge. next time in the same mode and settings.
▪ Falling edge: Indicates that the trigger signal is valid for falling edge. 3) Hardware “On” external trigger support can control the start
➢ Exposure Delay: Indicates the delay between when the camera receiving a trigger and end of image acquisition.
signal and when the camera captures an image. 4) Trigger module with external trigger overrides any resolution,
➢ Software Trigger mode: In the software trigger mode, click [Snap] and the bit depth, ROI and video recording settings.
camera is instructed to capture and output one image with each click.

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 Capture

Image Process
› Contents
ONLY FOR MONOCHROME CAMERA WITH COOLING
› General Introduction
➢ Frame Integral: Captures continuous multi-frame images according to the
› Starting Interface settings. Integration can be previewed in real time. Integration can improve image
brightness in low brightness situations.
› Windows ▪ Integral by Frames: Captures and averages the selected number of frames.
▪ Integral by Time: Captures and averages all frames over selected period of
› Capture
time.
› Image ▪ Preview: Displays the effect of the integration settings in real time, allowing
the user to make adjustments for the best results.
› Measure
Note: 1) Set an appropriate number of accumulated frames or the resulting image
› Report
may be too bright or distorted.
› Display 2) Frames and Time cannot be used simultaneously.

› Config
➢ Dark Field Correction: Corrects for variation in background non-uniformity.
› Info ▪ By default, Correction is disabled. It is only available only after the correction
coefficients are imported and set. Once imported and set, the box is
› Warranty automatically checked to enable the dark field correction.
▪ Click the [Correct] button and follow the pop-up prompt. Click next to
calculate the correction coefficient automatically.
▪ The default frame number is 10. Range is 1-99.
▪ Import and Export are to import/export correction coefficients, respectively.
▪ Repeat dark field correction whenever exposure time or scenes/samples are
changed.
▪ Closing the parameter group or software will remember the frame number.
Closing the software will clear the imported correction coefficient – this will
need to be imported again to enable correction.

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 Capture
Save Settings
› Contents CaptaVision+ provides the ability to save and recall imaging experiment parameters,
whether the camera is used for a different application or on a different platform.
› General Introduction Camera and imaging parameters (settings) can be saved, loaded and applied to new
experiments saving set up time, providing workflow efficiency and ensuring
› Starting Interface experiment process reproducibility and result generation.
All parameters mentioned previously in this manual can be saved except for flat field
› Windows
correction (this requires exact imaging conditions which are impossible to reproduce).
› Capture Parameter groups can also be exported for use on other computers for maximum
convenience to reproduce experimental conditions and generate uniform results
› Image across multiple platforms.
➢ Group Name: Enter the desired parameter group name in the text box and click
› Measure [Save]. This is the name you want to call this collection of settings. The computer
will show similar group names to avoid overwriting parameter files that have
› Report
already been saved.
› Display ➢ Save: To save the current parameters into a named parameter group file. Up to 50
sets of parameters can be saved.
› Config ➢ Load: Click the drop-down arrow to view previously saved parameter files, select
the parameter group for recall, then click [Load] to recall and apply those
› Info parameter settings.
➢ Export: Save the files of the parameter groups to another location (e.g., USB drive
› Warranty for import to another computer).
➢ Import: To load the selected files of parameter group from the selected folder.
➢ Delete: To delete the currently selected files of a parameter group.
➢ Reset all: Deletes all Parameter Groups and restores parameters to the factory
default.
➢ Load Fluorescence Mode: After clicking this button, the exposure mode is switched
to manual exposure, the exposure time is 100ms, and the automatic level is
automatically checked.

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 Capture

Light Frequency
› Contents
The frequency of the electric current can sometimes be observed in the live image.
› General Introduction Users can select a light source frequency that corresponds to the actual condition.
This will not correct for stroboscopic phenomena seen on live images. The default
› Starting Interface light source frequency is direct current (DC).

› Windows
Other Settings
› Capture
➢ Negative: Inverts the color of the current image.
› Image
➢ HDR: Click to stretch the dynamic range to reveal more image detail. Use as
› Measure needed for the application.

› Report

› Display Auto Focus (only for Auto Focus camera)

› Config ➢ Continuous Focusing: Select the area to be focused in the preview screen. The
camera will focus continuously on the selected area until it is in focus. When the
› Info
focal length is changed due to movement of sample or camera, camera will
automatically refocus.
› Warranty
➢ One-Shot AF: Select the area to be focused in the preview screen. The camera
will focus one time on the selected area. The focus position (focal length) will stay
unchanged until the user performs One-Shot AF again, or manually focuses using the
microscope.
➢ Focusing Location: Focusing location can be manually positioned. The focus
position (focal length) of camera will change according to location change.
➢ C-Mount: Automatically moves to the C interface position.

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 Image

› Contents
Control Interface
The following image processing functions are available: Image Adjust, Image Dye,
› General Introduction
Fluorescence, Advanced Computational Imaging, Binarization, Histogram, Smooth,
› Starting Interface Filter/Extract/Inverse Color.

Click to save picture in any format of JPG\TIF\PNG\DICOM; saving window will be

› Windows
popped out as shown below.
› Capture Note: DICOM is not available for MacOS.

› Image Click screenshot button on the right upper corner of the preview window to crop
picture, to select the interested area in preview image with mouse, then double left click
› Measure or double right click the mouse to complete screenshot. The screenshot will appear on
right picture bar, click to save current screenshot. If there is no need to save the
› Report
screenshot, right click to exit crop window.
› Display

› Config

› Info

› Warranty

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 Image

Image Adjust
› Contents
Adjust the image parameters to revise the effects of the captured images
› General Introduction
➢ Brightness: Allows adjustment of the image brightness, the default value is 0,
› Starting Interface adjusting range is -255~255.
➢ Gamma: Adjust the balance of darker and lighter regions on the monitor for
› Windows bringing out details; the default value is 1.00, adjusting range is 0.01~2.00.
➢ Contrast: The ratio between the darkest areas and brightest areas of the image,
› Capture
the default value is 0, adjusting range is -80~80.
› Image ➢ Saturation: The intensity of color, the higher value of the saturation, the more
intense the color, the default value is 0, adjusting range is -180~180.
› Measure ➢ Sharpen: Adjusts the appearance of edges in the image to appear more in focus,
may result in more vivid color in a specific area of the image. The default value is 0,
› Report
and adjusting range is 0~3.
› Display After completing the parameter adjustments for the image, Click [Apply As A New
Image] to accept all new settings and apply them to a copy the original image – this
› Config preserves the original image. The new image should be saved with a different file

› Info name to preserve the original image (data).

➢ Default: Click the [default] button to restore the adjusted parameters to the
› Warranty factory default.

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 Image

Image Dye
› Contents
Allows the user to apply color (false color or pseudo color) monochromatic images.
› General Introduction
Originating from a customer’s request, a user may select the desired color
› Starting Interface (representative of a selection of dyes), click [Apply As A New Image] to apply the
selected color to a copy of the original image. Click [Cancel] to cancel currently
› Windows applied color.
➢ Current: This window displays the currently available colors that can be selected
› Capture
by the user. Click to display the full color palette (Select Color) for a much
› Image broader selection of color choices. After selecting the color, click [OK] to accept the
color. Refer to the discussion on Capture > Fluorescence for more detail on
› Measure selecting and saving colors.
➢ Add to New Dye: To add selected colors on the palette into the new dyes.
› Report
➢ Dye Type: The user may be able to quickly select a color based on the
› Display fluorochrome used in the specimen staining process and apply that color to the
monochrome image.
› Config ➢ Cancel: To cancel a certain kind of dyes added through custom mode.

› Info

› Warranty

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 Image
Fluorescence

In the biological sciences, different fluorochromes are used to label different cell or
› Contents
tissue structures. Specimens may be labeled with as many as 6 or more fluorescent
probes, each targeting a different structure. The complete composite image of this
› General Introduction
type of specimen shows potential relationships between the stained tissue or
› Starting Interface structures. The spectral properties of fluorescent probes and the low efficiency of color
cameras don’t allow all of the probes in a specimen to be imaged simultaneously in one
› Windows color image. Therefore monochrome cameras (being more sensitive) are typically used,
and images of the specimen with illumination (and filters; the combination may be
› Capture referred to as “channels”) for the different fluorescent probes are used. The
Fluorescence module allows the user to combine these single channels, specific to a
› Image
single fluorescent probe, into one multi-color image representative of multiple probes.
› Measure
Operation:
› Report a) Select the first fluorescence image from the directory and open it,
b) Click the box next to [Start Color Composite] to start the process. The operating
› Display directions window will be displayed, as shown in figure(1).
c) Using the image gallery on the right, check an image to select it for combining, as
› Config
shown in the figure(2), then the combined image will display for you to preview, as
(1)
shown in the figure(3). Select other images with the same observation field as the first.
› Info
A maximum of 4 images can be combined.
d) Click [Apply As A New Image] to add the combined image to the image gallery. This
› Warranty
new image is displayed in the center workspace of the software interface, and the
fluorescence combining process is complete.

➢ Offset: Light traveling from the specimen to the camera can be shifted by mechanical
vibrations in the microscope system, or variations in the dichroic mirror or emission
filters from one filter set cube (channel) to another. This can lead to images that, when
combined, don’t overlap perfectly. Offset allows the user to correct any pixel drifting
by adjusting the X and Y position of one image in relation to another. One correction
(2) (3)
unit stands for one pixel. Click on [0,0] to restore to the original position.

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 Image

Advanced computational imaging

› Contents
CaptaVision+ software offers users three advanced post-process computational
› General Introduction image technologies that work by merging batches of images.

› Starting Interface ➢ Extend Depth of Field (EDF): Generates a 2-dimensional image using the in-focus
detail from a focus stack (multiple focus depths) from a sample. The module
› Windows
automatically creates a new image from a selection of images acquired at different
focus planes.
› Capture
➢ Image Stitching: Performs stitching of images acquired at adjacent fields from the
› Image same sample. Image frames should have approximately 20-25% overlap with an
adjacent image frame. The result is a large, seamless, high-resolution image.
› Measure ➢ High-Dynamic Range (HDR): This post-processing tool creates an image that
reveals more details in the sample. Basically, the module merges images acquired
› Report with different exposures (low, medium, high) into a new image with high dynamic
range.
› Display
(1)
› Config Operation:
1) Select the processing method to use by clicking the radio button next to it. A
› Info wizard function then guides the user through the process. The following describes
the process using EDF as an example:
› Warranty After selecting EDF, the first display window directs the user to select the images to
use in this processing application, as shown in the figure(1);
2) Click on [Combination] at the bottom of the interface;
3) The process may take some time to analyze and combine the images, and the
window displays the progress, for example: EDF 4/39
4) At the conclusion of the process, a thumbnail of the combined image is
generated and displayed in the left menu bar, as shown in the figure(2);
5) Click [Apply As A New Image] button and the new combined image is added to
(2)
the image gallery and displayed in the center workspace of the software interface,
and the combing process is complete.

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 Image

› Contents
Binaryzation

› General Introduction CaptaVision+ software can perform image binarization in which a full color sample can
be segmented and viewed as two classes according to differences in brightness. The
› Starting Interface user moves the threshold slider until the desired segmentation is observed – other
features are excluded. The grayscale value of the pixels of the image range from 0 to
› Windows
255, and by adjusting the threshold to observe one feature, the image is presented with
› Capture a distinctive black and white effect (based on the threshold, gray levels above the
threshold will appear as white, and those below will appear as black). This is often used
› Image
to observe and count particles or cells.
› Measure ➢ Default: Click the default button to restore the module's parameters to the factory
default.
› Report ➢ Apply: After making adjustments, click [Apply] to generate a new image, the new
image can be saved as desired.
Before
› Display
➢ Cancel: Click the Cancel button to stop the process and exit the module.
› Config After
› Info

› Warranty

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 Image

› Contents
Histogram

› General Introduction ➢ Color Scale Adjustment: Refine R/G/B color scales separately, then proportionally
redistribute the pixel value among them. The adjustment of picture’s color scale may
› Starting Interface highlight features and brighten the image – it may also darken an image. Each color
channel can be adjusted separately to change the color of picture in corresponding path.
› Windows
➢ Manual Color Scale: Users can manually adjust the dark shade (left color scale),
› Capture gamma and highlight brightness level (right color scale) to calibrate picture shade tone,
including contrast, shade and image hierarchy, and to balance the color of the picture.
› Image
➢ Automatic Color Scale: Check Automatic, customize the brightest and darkest pixel in
› Measure each path as white and black, and then proportionally redistribute the pixel values
between them.
› Report ➢ Apply: Apply current parameter setting in picture and generate a new picture. The
new picture can be saved as separately.
› Display
➢ Cancel: Click [Cancel] button to cancel parameter of the module.
› Config

› Info

› Warranty

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 Image

› Contents Smooth

› General Introduction CaptaVision+ software provides users with three image smoothing techniques for
reducing the noise in images, often improving the observation of detail. These
› Starting Interface computation techniques, often called “blurring”, include: ①Gaussian Blur, ②Box Filter,
and ③Median Blur.
› Windows
➢ Radius: Use the Radius slider to adjust the radius of the computational area for the
› Capture selected technique. The setting range is 0~30.
➢ Default: Click the [default] button to restore the module's parameters to the factory
› Image default.
➢ Apply: After selecting the desired smoothing technique and adjusting the Radius, click
› Measure
[Apply] to generate a new image using those setting, and the new image can be saved as
› Report desired.
➢ Cancel: Click the [Cancel] button to stop the process and exit the module.
› Display

› Config

› Info

› Warranty

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 Image

› Contents
Filter/Extract/Inverse Color

› General Introduction CaptaVision+ software allows users with methods to Filter/Extract/Inverse Color
in previously acquired still images (not videos) as needed for the application.
› Starting Interface
➢ Color: Select Red/Green/Blue.
› Windows ➢ Filter Color: Can be useful to check the color level information in each channel of a
color image and combine images with complementary colors. The combined image will
› Capture always be brighter. The filter selectively removes the selected color from the image.
➢ Extract Color: Extract the certain of color from the RGB color group. Extract removes
› Image
the other color channels from the image, keeping only the color that was selected.
› Measure ➢ Inverse Color: Invert the colors in the RGB group to their complementary colors.
➢ Apply: After choosing the settings, click [Apply] to apply those settings to a copy of
› Report the original image and generate a new image, then save the new image as desired.
➢ Cancel: Click the [Cancel] button to cancel process and exit the module.
› Display

› Config Original Filter Blue Extract Blue

› Info

› Warranty

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 Image

› Contents
Deconvolution

› General Introduction Deconvolution may aid in reducing the impact of artefacts in an image.
➢ Iterations: Choose the number of times to apply the algorithm.
› Starting Interface ➢ Kernel Size: Define the size of the kernel (“field of view” of the convolution) for the
algorithm. A lower value uses fewer nearby pixels. A higher value extends the range.
› Windows
➢ Calculation: After choosing the Kernel Size and the number of Iterations, click
› Capture [Calculation] to see the result on the image.
➢ Apply: After Calculation, click [Apply] to apply the deconvolution process to the
› Image
image. This cannot be undone.
› Measure ➢ Cancel: Click [Cancel] to cancel or undo the calculation process.

› Report Note: Be sure to click to save the image and name it accordingly.

› Display

› Config (before)

› Info

(after)
› Warranty

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 Image

Automatic Counting
› Contents
➢ Start Counting: Click button to begin automatic counting mode.
› General Introduction
➢ Region: All: Selects the entire image for the counting area.
› Starting Interface ➢ Region: Rectangle: Choose the Rectangle to define a rectangular area in the image
for counting. Left-click to select two endpoints to draw a rectangular shape on the
› Windows image.
➢ Region: Polygon: Choose Polygon to select an area that cannot be adequately
› Capture
selected using the Rectangle option. Left-click multiple times to place corners of a
› Image polygon on the image. Double-click to end the drawing.
➢ Restart Counting: Clears the region and returns to the Start Counting interface.
› Measure ➢ Next: Advances to the next step.

› Report
Segmentation
› Display ➢Auto Bright: Automatically segments bright objects from the dark background.
➢ Auto Dark: Automatically segment dark objects from bright background.
› Config
➢ Manual: Manual segmentation is based on the histogram distribution of the image,
› Info which can be adjusted by dragging the two vertical lines on the left and right in the
histogram, by adjusting the lower and upper limit values using the up/down arrows, or
› Warranty by directly entering the upper and lower limits in the boxes.

Morphological Processing
➢ Dilate: Change the size of the cells in the image to expand the borders of bright cells
and shrink the borders of dark cells.
➢ Erode: Change the size of the cells in the image to expand the borders of dark cells
and shrink the borders of bright cells.
➢ Open: Change the distinction between cells. For example with a bright cell on a dark
background, clicking Open will smooth the cell boundary, separate connected cells, and
remove small black holes in the cell.
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 Image

› Contents
➢ Close: The opposite of Open above. For example with a bright cell on a dark
› General Introduction
background, clicking Close will fill the gap of a cell, and may stretch and highlight an
› Starting Interface adjacent cell.
➢ Fill holes: Fills holes in cells in the image.
› Windows ➢ Restart Counting: Clears the region and returns to the Start Counting interface.
➢ Back: Goes back to the previous operation process.
› Capture
➢ Next: Advances to the next step.
› Image
Display
› Measure ➢ Contour: Use contour lines to represent the divided cells.

› Report ➢ Region: Use padding to represent divided cells.

› Display Cutting Mode

➢ Auto Cut: Draws cell boundaries according to the contour of the cell.
› Config
➢ Manual: Manually select multiple points on the image to separate cells.
› Info ➢ No Cut: Do not divide the cells.
➢ Merge: Merge separate cells into one cell.
› Warranty
➢ Include Boundary: When calculating the number of cells, cells with incomplete
boundaries in the image will not be counted.

➢ Restart Counting: Clears the region and returns to the Start Counting interface.
➢ Back: Goes back to the previous operation process.
➢ Next: Advances to the next step.

Continued on next page →

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 Image

› Contents
Target Data Settings:
› General Introduction
➢ Add: Add the type of calculation from the Target Data Settings to the statistical
› Starting Interface result.
➢ Delete: Remove a type of calculation.
› Windows ➢ Minimum: Set the minimum value for each Data Type for separated cells. Cells
smaller than the minimum value will not be counted.
› Capture
➢ Maximum: Set the maximum value for each Data Type for separated cells. Cells
› Image greater than the maximum value will not be counted.
➢ OK: Start counting cells according to the criteria.
› Measure
NOTE: Refer to the tabs below the preview window to see the results of automatic
› Report counting. The Automatic Counting Table shows the calculations according to the Target
Data Settings. Default settings are Area, Roundness, Long Axis and Short Axis.
› Display Automatic Counting Statistics shows the statistical values for each of the Target Data
Settings above.
› Config ➢ Draw Target: check this option after the automatic counting is completed. Allows
you to manually draw the missed cell on the interface.
› Info
➢ Export Report: Export statistical cell data to Excel file. The exported data is inclusive
› Warranty of the Automatic Counting Table and Automatic Counting Statistics.
➢ Restart Counting: Clears the region and returns to the Start Counting interface.
➢ Back: Goes back to the previous operation process

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 Image

› Contents
Automatic Counting Property
Adjust the properties of the text and drawings/borders in the image during Automatic
› General Introduction
Counting.
› Starting Interface ➢ Font: Set font and size, default is Arial, 9, click to open the font menu to select
the desired font.
› Windows ➢ Font Color: Set font color, default is green, click to open the color palette to
select the desired color.
› Capture
➢ Target Color: Set cell display target color, the default is blue, select it and click
› Image to open the color palette to select the desired color.
➢ Contour Width: Adjust the cell display outline width, default is 1, range 1~5.
› Measure ➢ Precision: Set the number of decimal places for the maximum and minimum values.

› Report By default, 3 decimal places are reserved. The range is 0~6.

› Display

› Config

› Info

› Warranty

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 Measure
Control Interface
CaptaVision+ provides tools for measuring features in images. Measurements are
› Contents typically performed on saved, static images, but CaptaVision+ allows the user to
perform measurements on live previews of samples to provide real-time
› General Introduction information of the sample. CaptaVision+ provides a rich set of measurements for
image analysis. The principle of the measurement functions is based on image
› Starting Interface
pixels as the basic execution unit and, with calibration, the resulting measurements
› Windows can be very accurate and repeatable. For example, the length of the line feature is
determined by the number of pixels along the line, and with calibration, pixel-level
› Capture measurements can be converted to more practical units such as millimeters or
inches. Calibration is performed in the Calibration module.
› Image

› Measure Measure Tool

› Report Begin all measurements by clicking the desired measure tool in the module window.
Line: Click in the image to draw a line segment graphic and complete the
› Display drawing with another click. Arrows are displayed at the endpoints.
H Shape Straight Line：Draw a line segment graphic and then finish drawing
› Config with one more click, vertical lines at endpoint.
Three Dots Line Segment: Draw graphic with three dots line segment, finish
› Info
drawing when click for the third time.
Multiple Dots Line Segment: Draw graphic with multiple dots at the same
› Warranty
direction,
single click to draw and double click to end drawing.
Parallel Line: Click in the image to draw a line segment, left click again to draw
its parallel lines, then double-left-click to finish drawing.
Vertical Line: Click in the image to draw a line segment, left click again to draw
its vertical line, then double-left-click to finish drawing.
Polyline: Click in the image and draw a line segment, left click again to add a
new line segment to the existing polyline, then double-left-click to finish drawing.

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 Measure
Measure Tool (continued)

› Contents Rectangle: Click in the image to start drawing, drag the shape down and to
the right, then double-left-click to complete the drawing. Measurements include
› General Introduction length, width, perimeter and area.
Polygon: Click in the image to start drawing the shape, left click to draw each
› Starting Interface additional face, then double-left-click to finish drawing.
Ellipse: Click in the image, drag the shape down and to the right, then double-
› Windows
left-click to finish. Measurements include perimeter, area, major axis, short axis,
› Capture and eccentricity.
Roundness: Select the first and second segment passing through the center
› Image of the circle on the contour of the measured circle. After drawing, the value of the
degree to which the cross section is close to a theoretical circle is obtained (closer
› Measure to “0” means closer to perfect circle.
Radius Circle: Click in the image to select the center of the circle, click again
› Report
to define the radius length, then click again to finish drawing. 3Point Angle: Click to set the end point of one arm of the
Diameter Circle: Click in the image, drag to enlarge the circle, then click again angle, click to set the vertex (inflection point), then click again
› Display
to finish drawing. after drawing the second arm and to finish drawing.
› Config 3Point Circle: Click in the image to define one point on the perimeter, move 4Point Angle: Click in the image the angle between two
and click to set another point, then move and click a third time to finish drawing. unconnected lines. Click to draw the endpoints of the first line,
› Info Concentric Circles: Click in the image to draw the first circle with its radius, in then click to draw the endpoints of the second line. The software
or out and click to define the next circle, then double-click to finish drawing. will extrapolate and determine the smallest angle between the
› Warranty 4Point Double Circle: (like drawing two radius circles) Click to position the two lines.
center of the first circle, then click to define the radius of the first circle. Click Dot: Click in the image where you want to place a dot e.g., for
again to position the center of the second circle, then click again to define the counting or to mark a feature. Displays XY coordinate of the dot.
radius of the second circle. Free Draw: Click in the image and draw line of any shape or
6Point Double Circle: (like drawing two 3point circles) Click three times to length.
select three points on the first circle, and click another three times to select the Arrow: Click in the image to start the arrow, click again to end
three points of the second circle, then end the drawing. the drawing.
Arc: Click in the image to select the starting point, drag and click again to set Text: Click in the image and type to add a text note.
the second point on the arc, then click again to finish the drawing. All 3 points will
be on the arc. Continued on next page →

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 Measure

› Contents
Measure Tool
Within graphics drawing mode, right-click the mouse to switch to the selecting mode.
› General Introduction Right-click again to return to the drawing mode.

› Starting Interface Select: Click in the image window to select an object or annotation. The
mouse cursor changes to , use the to move the object or annotation.
› Windows Delete: To delete the drawing, measurement or annotation.
Undelete: Undo the last delete operation.
› Capture
Clear All: Delete all the drawn and measured graphics or texts on the current
› Image layers.
Combine: When saving the image, the drawings, measurements and annotations
› Measure will be permanently added (“burned into”) the image. By default, Combine is active.
➢ Data Type: Each graphic has its own available data types to display, such as length,
› Report
perimeter, area, etc. (see example to right of the available Data Types when the
› Display Ellipse tool is selected). When drawing the graphic, the data will also display. Hover
the cursor over the data display for a graphic and right-click the mouse to display the
› Config
Data Type options to choose to display for that graphic.
› Info When the mouse is in the state, use the mouse scroll wheel to zoom in/out on
the image. Hold down the left mouse button to drag/reposition the drawn graphic or
› Warranty annotation. Position the cursor on an endpoint of a graphic , then click and drag
to change the shape or size of the graphic.
When the mouse is in the state, use the mouse scroll wheel to zoom in/out on
the image. Place the cursor on a graphic and click and drag to move the image.
Position the cursor on an endpoint of a graphic, then click and drag to change the
shape or size of the graphic.
All drawing and measurement graphic data will be added to the measurement table.
Click [Export to Excel] or [Export to TXT] to transfer the data information to EXCEL
form format or TXT document format. Click [Copy] to copy the entire table for pasting
into another document.

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 Measure
Calibration

When performing calibrations, it is recommended to use a stage micrometer or other

› Contents
device with standardized measurement markings.
› General Introduction ➢ Create a calibration table: Saves a series of measurements used to convert the
number of pixels into standard units of measure. Click [Draw], draw a straight line on
› Starting Interface the image (press [ctrl] to draw horizontal or vertical lines). If using a stage micrometer,
begin on the left side of the micrometer, click on the left edge of a tick mark and, for
› Windows
maximum accuracy, drag the line to the far right of the images, then click on the left
› Capture edge of another tick mark (see figure(1)). Enter the actual Length of the object in the
image. Enter a logical Name for the calibration measurement (e.g., “10x” for a
› Image measurement with a 10x objective), confirm the unit of measure, then finally, click
[Apply] to accept the entries and save the calibration.
› Measure
Note: Acceptable units of measure: nm, μm, mm, inch, 1/10inch, 1/100inch, 1/1000inch.
› Report ➢ View/Edit Calibration Table: Multiple groups of calibrations can be created to
facilitate measurements under different application scenarios. The individual
› Display calibrations can be viewed and edited in the calibration table as shown in the figure (2).
➢ To change to a different calibration (e.g., after changing objective magnification), (1)
› Config
click in the checkbox in the [Current] column next to the desired calibration, then apply
› Info this calibration to new measurements on images acquired at that magnification.
➢ Select a calibration in the table and right-click to open the file options window (see
› Warranty figure (3)). Click [Delete] to delete the selected calibration – the currently active
(checked) calibration cannot be deleted while active. Click [Load] to locate and import
a previously saved calibration table. Click [Save As] to save and export the entire (2)
calibration table with an assigned name for future recall and loading.
Resolution is the preview resolution of the new calibration ruler. Switching the
resolution, calibration ruler and measurement data will be automatically converted.
Note: Calibration processing can be performed more accurately with a micrometer.
Using an incorrect calibration table will cause inaccurate measurements. Special (3)
attention must be used to select the correct calibration table before making
measurements on images.
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 Measure
Calibration

Calibrations can be easily exported and imported in case of changing

› Contents
computers or for use on identical imaging configurations.
› General Introduction 1. After calibrating the camera for the objectives, click on any of the
calibrations in the calibration table to activate it (it will appear
› Starting Interface highlighted in blue). Right-click the mouse and choose “Save As”..
2. Choose the location where the calibration file will be saved and click
› Windows
“Save”. The file will be saved as type “.ini”.
› Capture 3. To import the calibration file, navigate to the Calibration Table in the
Measurement section of CaptaVision+, and click on the default
› Image calibration to activate it (it will be highlighted in blue). Right-click the
mouse and choose “Load”.
› Measure
4. In the pop-up window, navigate to the location where the calibration
› Report file was saved. The dialog window will filter to show only “.ini” files.
5. Select the calibration file to import and click “Open”.
› Display 6. Confirm that the calibrations have been loaded into the table.

› Config
NOTE: It is NOT RECOMMEMDED to use the same calibration data between
› Info microscopes and cameras. Despite microscopes and cameras similarities
and even identical configurations, minor variations in magnification are
› Warranty present, thereby invalidating the calibrations if used on instruments other
than on which the calibrations were first measured.

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 Measure
Measure Layer

› Contents ➢ Multiple layers can be created on the image allowing multiple measurement
approaches to be created, applied or shown individually or in multiples.
› General Introduction This layer creation module meets the needs of many image measuring and image
processing applications by allowing quick access to measurements depending upon the
› Starting Interface
image, magnification or application.
› Windows
Once a measurement has been made, the layer creation function automatically assigns

› Capture the original image without measurements as “Background”, then names the
measurement layer as “Layer 01”, which will show the corresponding measurement
› Image results.

› Measure Click the checkbox in the [Current] column to activate a layer for measurement.
Measurements made on that layer will be associated with that layer.
› Report
Measurement data from different layers may be displayed individually by layer or by
› Display multiple layers. Click the checkboxes in the [Visible] column of the layers you wish to
display.
› Config
Click [New] to create a new layer. The default layer naming convention is to augment
› Info the suffix of the layer by 1 as “Layer 01”, “Layer 02”, “Layer 03”, and so on.

› Warranty Rename a layer two ways. When a layer is Current, click the [Rename] button and
enter the desired name for the layer. If a layer is not Current, click the layer name in
the [Name] column (it will highlight in blue), click [Rename] and enter the desired name
for that layer.

Click [Delete] to delete the selected (checked) layer.

Click [Rename] to rename the selected (checked) layer or selected layer name.

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 Measure
Metrics Flow

The Metrics Flow feature of CaptaVision+ provides powerful, semi-automated

› Contents
measurements especially for pass-fail quality inspection of devices or parts in industrial
› General Introduction manufacturing environments. Metrics Flow adds convenience and improves speed and
accuracy of inspection.
› Starting Interface 1) Open a group of device or part images saved in the image gallery.
2) Select the image of the standard sample to calibrate and set the tolerances for later
› Windows
measurements and observations; this will be called the reference image in this manual.
› Capture 3) Click [Start Building A Metrics Flow] checkbox to create a new metrics template.
4) Use the various measurement and annotation tools to measure or draw any desired
› Image shape(s) on the reference image previously opened. The software will record the entire
measuring process and save the measurement results or drawn graphics as the
› Measure
reference specifications, as shown in figure (1).
› Report 5) After recording the reference measurements and annotations on the template,
assign a name to the template and click [Save].
› Display
6) Click [Start Applying A Metrics Flow], select the created template, click the [Run]
› Config button to apply the template, click [Delete] to delete the template.
7) Select the image for inspection/observation and follow the steps as when creating
› Info the template. Draw the first measurement. The Metrics Flow will automatically
advance to the next measurement tool. Continue until every measurement in the flow
› Warranty has been made.
8) After the software applies the template, the [Run] button will be released and a (1)
window showing the results is displayed, as shown in figures (2) (3).
9) Click [Export to PDF/Excel] to save the results in PDF format or export in Excel format
with the detecting results.
10) Continue to click [Run] and select other images for inspection/observation, then
repeat steps 7, 8, and 9 as above.
11) After finished analyzing all the images, click [Stop Applying A Metrics Flow] to stop
(2) (3)
the Metrics Flow process.

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 Measure

Graphics Properties
› Contents CaptaVision+ allows users to manage and adjust graphics properties for their
application.
› General Introduction ➢ Create or change a name in the blank text field in the Value column next to the
Name row.
› Starting Interface
➢ Show Name: Check the False checkbox if you do NOT want the Name to be
› Windows displayed.
➢ Precision: Choose the precision (characters after the decimal point) of any values
› Capture being displayed. The default value is 3, the range is 0~6.
➢ Line Width: Adjust the width of the current measurement tools on the image.
› Image
The default is value 1, the range is 1~5.
› Measure ➢ Line Style: Select the line style of the current measurement tools on the image.
The default style is a solid line. Other available styles are dashed lines, dotted lines,
› Report and double dotted lines.
➢ Graphics Color: Select the color of the lines of the measurement tools on the
› Display
image. The default color is red; other colors can be chosen by clicking the color box
› Config and then the button.
➢ Font: Choose the text font for the current measurement data. The default format
› Info is [Arial, 20]. Click the “A” in the Font:Value field to select another font and/or size.
➢ Font color: Choose the color for the current measurement data on the image.
› Warranty
The default color is blue; other colors can be chosen by clicking the color box and
then the button.
➢ No Background: Check or uncheck the checkbox next to True. Checked box =
transparent (no) background; unchecked box = with background. Transparent
background is the default setting.
➢ Background Color: Select the background color for the current measurement data
on the image. Click the color area and then the button to choose the desired
background color, the default background color is white.
➢ Apply to All: Apply all the graphics properties to the measurement graphics.
➢ Default: Revert to and apply the default graphics settings.

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 Measure
Manual Class Counting

› Contents The Manual Class Counting function allows the user to manually count objects in the
sample (e.g., cells) based on a feature or detail. Multiple features (Classes) may be
› General Introduction specified based on color, morphology, etc. as needed for the user’s application. Up to
seven classes are possible.
› Starting Interface
➢ Name: Double-click the category button (e.g., Class1) to name the category.
› Windows ➢ Color: Double-click the color dot in the Color column to select another color for the
class.
› Capture ➢ Size: Double-click the number to set the size of points. Default is 10; range is 1-500.
➢ Click [Add New Class] to create a new class.
› Image
➢ Click [Delete Class] to remove a class from the lists.
› Measure ➢ Click [Undo] to undo the last action.
➢ Click [Clear All] to clear all classes in the table in one click.
› Report
➢ Click the [Start Class Counting] checkbox to select a class to use, then left-click the

› Display mouse on targets in the image to count. The counted results are automatically
displayed in the class counting table, as shown in figure(1) and figure(2).
› Config (1)
After counting is finished with one or more classes, the counting results are displayed in
the counting table. Export the data by selecting [Export to Excel] (see figure(2)), then
› Info
choose the destination in which to save the file.

› Warranty

(2)

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 Measure

Scale Property
› Contents
CaptaVison+ allows users to set the scale properties based on need or application.
› General Introduction
➢ Show Scale: Click the checkbox to display the scale bar on the image. The default setting is to NOT
› Starting Interface display the scale bar. When displayed, the scale bar will automatically be placed in the top-left of the
image. Use the mouse to drag the scale bar to another position anywhere on the image.
› Windows ➢ Type: Select Manual or Automatic display type. The default is automatic.
➢Click on the Value side to open dropdown list to choose Auto or Manual
› Capture
➢ Align: Sets alignment of the value to the scale. Choose left, center, and right alignment. Default is
› Image center.
➢ Orientation: Set the display direction of the current scale. Choose horizontal or vertical. Default is
› Measure horizontal.
➢ Name: Create name for the scale in the current image. The default setting is blank.
› Report
➢ Length: The default value is 100 units, according to the calibration file selected. After choosing
› Display Manual for the Type (see above), the Length value can be modified by entering new value.
➢ Color: Choose the line color for the current scale bar on the image. The default color is red; other
› Config
colors can be chosen by clicking the color box.
› Info ➢ Width: Adjust the width of scale bar on the image. The default is value 1, the range is 1~5.
➢ Text Color: Choose the color for the current scale bar on the image. The default color is red; other
› Warranty colors can be chosen by clicking the color box.
➢ Text Font: Choose the text font for the current scale bar. The default format is [Arial, 28]. Click the
“A” in the Font:Value field to select another font and/or size. Background Color: Select the background color for the
➢ Border Color: Choose the color for border of the scale currently displayed on the image. The scale on the image. The default color is white; click the
default color is red; other colors can be chosen by clicking the color box. color box to choose another background color.
➢ Border Width: Adjust the width of the border surrounding the scale. The default value is 5, range ➢ Apply to All: Apply settings to all scales
1~5. ➢ Default: Revert to and apply the default settings for
➢ No Background: : Check or uncheck the checkbox next to True. Checked box = transparent (no) the scale on the image.
background; unchecked box = with background. Transparent background is the default setting.

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 Measure

Ruler Property
› Contents
CaptaVision+ allows users to set the ruler properties according to need or application.
› General Introduction ➢ Show Ruler: Click the checkbox to display the crosshair-style ruler on the image.
The default setting is unchecked to not display the crosshair.
› Starting Interface
➢ Unit Interval: Set and apply cross-ruler interval distance on the image.
➢ Ruler Height: Set and apply height of the cross-ruler on the image.
› Windows
➢ Ruler Color: Choose the color for the current crosshair on the image. The default
› Capture color is black; other color options are available by clicking the color box.
➢ Ruler Font: Set the font and font size for the crosshair.
› Image ➢ No Background: Uncheck the checkbox for a transparent background. Check the
checkbox to apply a background to the ruler. The default setting is a transparent
› Measure background.
➢ Background Color: Select the background color for the current ruler displayed on
› Report
the image. Click the color box to choose another background color. The default
› Display background color is white.
➢ Default: Revert to and apply the default ruler settings.
› Config

› Info

› Warranty

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 Measure

Grid Property
› Contents
CaptaVision+ allows users to set the grid properties on the image according to need
› General Introduction or application. The grid is simply a series of vertical and horizontal lines dividing the
image into rows and columns.
› Starting Interface ➢ Show Grid: Check the Show Grid checkbox to display the grid on the image. The
default setting is to NOT show the grid.
› Windows ➢ Type: Select the way to define the grid to apply to the current image, either by Line
Number or Line Interval.
› Capture ➢ Row/Column: When Type is defined as Line Number, enter the number of
horizontal (row) lines and vertical (column) lines to show on the image. The default is
› Image 8 for each.
➢ Line Interval : If you choose to define the grid by the line interval, you can enter
› Measure the number of grids you need into the blank of Line Interval, the default number of
line interval is 100.
› Report ➢ Line style: Choose the line style for the grid to apply on the image there are 5 styles
of grid could be chosen from, the solid lines, dashed lines, dotted lines, dotted lines,
› Display and two dotted lines.
➢ Line Color: Choose the color for the grid to apply on the image, the default color is
› Config
red, Click on […] to choose the desired grid color.
➢ Default: Resort and apply the default parameters settings to the grid on the image.
› Info

› Warranty

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 Measure

Save Settings
› Contents
Copy the parameter file and load it on another computer. By transferring parameters
› General Introduction between platforms and imaging systems, the user's experimental conditions are kept
as consistent as possible.
› Starting Interface
➢ Group Name: Set the parameter name, it can also be viewed and loaded through
the drop-down menu.
› Windows
➢ Save: Click [Save] to save the settings.
› Capture ➢ Load: Click [Load] to load the selected settings Group into CaptaVision+.
➢ Delete: Click [Delete] to permanently remove the selected settings file.
› Image ➢ Export: Click [Export] the selected settings file.
➢ Import: Click [Import] to add a saved settings file into the Group drop-down menu.
› Measure ➢ Reset All: Clear all user settings and restore to the software factory settings

› Report

› Display

› Config

› Info

› Warranty

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 Measure

Fluorescence Intensity
› Contents
CaptaVision+ allows users to measure the gray value of the image using a line or Note:
› General Introduction rectangle. • Draw a line across the image and complete the drawing with a
➢ Switch from the preview mode to measurement mode, or open an image, and second click. A plot of fluorescence intensity across the line
› Starting Interface will be displayed.
check [Start] to enable the function. Uncheck [Start] to reset.
➢ Select Line or Rectangle for the shape from which to measure gray values. • To draw a rectangle, left-click to select two endpoints to draw
› Windows
➢ Draw a line or rectangle to select the area for gray value measurement. Left-click a rectangle graph, which can measure the average
› Capture the mouse to set the starting point. Drag the mouse to draw the line or rectangle. fluorescence intensity data of each column of pixels within the
Left-click the mouse again to stop drawing and display the fluorescence intensity in rectangle.
› Image the chart.
➢ Click [Save] to save the current measurement data in Excel format to the local hard
› Measure drive.

› Report

› Display

› Config

› Info

› Warranty

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 Measure

Cursor Property
› Contents
The user can adjust the properties of the measurement cursor based on need or
› General Introduction preference. The setting interface is shown to the right.
➢ Width: Sets the thickness of the cross cursor line segment. Setting range is 1~5,
› Starting Interface
and the default value is 2.
➢ Cross Style: Set the line style of the cross cursor. Choose either solid or dotted line.
› Windows
The default is solid line.
› Capture ➢ Cross Length: Select the length (in pixels) of the cross cursor currently displayed on
the image. The default is 100.
› Image ➢ Pickbox Length: Select the width and length of the cross cursor that is currently
displayed on the image, the default is 20 pixels.
› Measure ➢ Color: Select the line color of the currently applied cross cursor on the image. Click
the color box to open a dialog box with a color palette to select the desired color.
› Report

› Display

› Config

› Info

› Warranty

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 Report

CaptaVision+ provides report formats to export measurement data to working report

› Contents documents. Reports can also be exported in real time when in the preview window.
Custom templates allow users to modify the report for specific needs and are only
› General Introduction
supported in Excel format.
› Starting Interface
Template Report
› Windows
Use to export custom measurement templates, measurement data modules and batch
› Capture export reports.
➢ Report Templates: Choose the desired report template from the dropdown list.
› Image
➢ Add: Add a custom template. The custom template must be modified from the
› Measure default template and the final template format is Excel. The default template is in the
[templates] file under the software installation path. Use the # identifier to indicate the
› Report content that needs to be displayed. When the ## identifier appears, it means that the
header of the data table is hidden.
› Display
➢ Delete: Delete the selected template.
› Config ➢ Open: Preview the selected template.
➢ Export Report: Export the current report, the format is Excel.
› Info ➢ Batch Export: Check [Batch Export], the user can select the pictures to be exported,
then click [Batch Export] to export the report. The image name are searchable.
› Warranty

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 Report

CaptaVision+ provides user the convenience to export measurement data as a report

› Contents document.
➢ Report Templates: Choose the desired report template.
› General Introduction
➢ Project Name: Enter a customized name for the project. This name will appear on
› Starting Interface the report.
➢ Sample Name: Enter the name of the sample in this project. This name will appear
› Windows
on the report.
› Capture ➢ User Name: Enter the name of the user or operator.
➢ Notes: Enter any notes that provide context, supplement and detail for the project.
› Image ➢ Image Name: Enter the file name of the image referenced in this report. The image
can the be automatically loaded into the report.
› Measure
➢ Image information: Click the Image Information checkbox to show information of the
› Report image selected above. Uncheck the checkbox to hide the image information.
➢ Measure Data: Click the checkbox to display and include in the report the
› Display measurement data table for the selected image.
➢ Class Counting: Click the checkbox to display and include in the report the class
› Config
counting table for the selected image.
› Info ➢ Export Report: Export current report into a PDF document.
➢ Print: Print the current report.
› Warranty ➢ Cancel: Cancels the report creation operation. All entries are cleared.

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 Display

➢ Zoom In: Magnify the current image and display it larger than its original size.
› Contents
➢ Zoom Out: Reduces the current image and displays it smaller than its original size.
› General Introduction ➢ 1:1: Displays the image in its 1:1 original size.
➢ Fit: Adjusts the display size of the image to fit the software operating window.
› Starting Interface
➢ Black Background: The image will be display in full screen and the background of the image is
› Windows black. Press [ Esc ] button of the computer keyboard or click on the Back Arrow symbol
on the lower right corner of the software window to exit the black background mode.
› Capture ➢ Full Screen: Displays the image in a full screen. Press the [ Esc ] button of the computer

› Image keyboard or click on the Back Arrow symbol on the lower right corner of the software
window to exit the full screen mode.
› Measure ➢ Horizontal Flip: Flips the current image horizontally, like a mirror (not rotation).

› Report ➢ Vertical Flip: Flips the current image vertically, like a mirror (not rotation).
➢ Rotate 90°: Rotates the current image clockwise 90°degrees with each click.
› Display

› Config

› Info

› Warranty

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 Config

› Contents

› General Introduction

› Starting Interface

› Windows

› Capture

› Image

› Measure

› Report

› Display

› Config

› Info Capture / Image / Measure

Use Config to show/hide and order software functions
› Warranty
➢ Visible: Use the checkboxes in the Visible column to show or hide a function module in
the software interface. A checked box indicates that the module will be visible. All
modules are checked by default. Use this function to hide modules that are not used.
➢ Up: Click the up arrow to move the module up in the list of modules displayed in the
software interface.
➢ Down: Click the down arrow to move the module down in the list of modules displayed
in the software interface.

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 Config

› Contents
JPEG
› General Introduction
Jpeg image format size can be preset in CaptaVision+. When Jpeg is selected as the image
› Starting Interface type in the file saving function, the image size will be generated according to the set
format when taking pictures.
› Windows Default: When Default is selected, the generated image keeps the current camera image
resolution.
› Capture
➢ Resize: When selected, image dimensions can be specified by the user.
› Image ➢ Percentage: Choose Percentage to adjust image dimensions using a percentage of the
original image dimensions.
› Measure ➢ Pixel: Choose Pixel to specify the number of pixels in the horizontal and vertical
dimensions of the image.
› Report
➢ Horizontal: Enter the desired size of the image in the horizontal (X) dimension.
› Display ➢ Vertical: Enter the desired size of the image in the vertical (Y) dimension.
➢ Keep Aspect Ratio: To prevent image distortion, check the Keep Aspect Ratio box to lock
› Config the aspect ratio of the image when setting the size.

› Info

› Warranty

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 Info

› Contents Preferences
➢ Language: Select the preferred software language. The software must be
› General Introduction
restarted to take the language setting into effect.
› Starting Interface ➢ Microscope:
• Biological. The default is to use automatic white balance with gamma
› Windows value 2.10 and the mode of exposure to the right.
• Industrial. The default color temperature value is set to 6500K.
› Capture
CaptaVision+ is set to use area white balance with a gamma value of 1.80
› Image and middle exposure mode.
The software will need to be restarted for any changes to Preferences to take effect.
› Measure

› Report Help
› Display The Help feature displays the software instruction manual for reference.

› Config
About
› Info The About dialog displays more information about the software and operating
environment. Information may include the connected camera model and operating
› Warranty
status, the software version and operating system information.

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 Info

› Contents About
The About dialog displays more information about the software and operating
› General Introduction
environment. Information may include the connected camera model and operating
› Starting Interface status, the software version and operating system information.

› Windows

› Capture

› Image

› Measure

› Report

› Display

› Config

› Info

› Warranty

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 Limited Warranty

› Contents Digital Cameras for Microscopy

This digital camera is warranted to be free from defects in material and workmanship for a period of one (1) year from the date of invoice
› General Introduction
to the original (end user) purchaser.
› Starting Interface This warranty does not cover damage caused in-transit, damage caused by misuse, neglect, abuse or damage resulting from either
improper servicing or modification by other then ACCU-SCOPE or UNITRON approved service personnel. This warranty does not cover any
› Windows routine maintenance work or any other work that is reasonably expected to be performed by the purchaser. No responsibility is assumed
for unsatisfactory operating performance due to environmental conditions such as humidity, dust, corrosive chemicals, deposition of oil
› Capture
or other foreign matter, spillage or other conditions beyond the control of ACCU-SCOPE Inc. This warranty expressly excludes any liability
› Image by ACCU-SCOPE INC. and UNITRON Ltd for consequential loss or damage on only grounds, such as (but not limited to) the non-availability
to the End User of the product(s) under warranty or the need to repair work processes.
› Measure All items returned for warranty repair must be sent freight prepaid and insured to ACCU-SCOPE INC., or UNITRON Ltd., 73 Mall Drive,
Commack, NY 11725 – USA. All warranty repairs will be returned freight prepaid to any destination within the Continental United States of
› Report
America. Charges for repairs shipped back outside this region are the responsibility of the individual/company returning the merchandise
› Display for repair.

› Config To save your time and expedite service, please prepare the following information in advance:

› Info 1. Camera model and S/N (product serial number).

2. Software version number and computer system configuration information.
› Warranty 3. As much detail as possible including a description of the problem(s) and any images help to illustrate the issue.

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