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Eppendorf Reference® 2

Service manual - English

Copyright© 2020 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of
this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf Brand Design are registered trademarks of Eppendorf AG, Germany.

epT.I.P.S.®, and Reference® 2 are registered trademarks of Eppendorf AG, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

U.S. Patents are listed on www.eppendorf.com/ip

4920 920 018-03/072020

 Table of contents
Eppendorf Reference® 2 3/72
English (EN)

Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Purpose and scope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4.1 Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4.2 Scope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5 Version overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Product overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.1 Pipette models. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Information on liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Setting the volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2 Attaching the pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.3 Optimum immersion depths . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4 Forward pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4.1 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4.2 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4.3 Ejecting the pipette tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.5 Reverse pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5.1 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5.2 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5.3 Ejecting the pipette tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.6 Storing the pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Control button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.3 Pipette tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4 Tip cone. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

6 Disassembly/assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.1 Disassembling the single-channel pipette lower part up to 1000 μL . . . . . . . . . . . . . . . . . . . . . 19
6.2 Assembling the single-channel pipette lower part up to 1000 μL . . . . . . . . . . . . . . . . . . . . . . . 20
6.3 Disassembling the lower part of the single-channel pipette from 2 mL upward . . . . . . . . . . . . 21
6.4 Assembling the lower part of the single-channel pipette from 2 mL upward . . . . . . . . . . . . . . 22
6.5 Disassembling the lower part of the multi-channel pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.6 Assemble the lower part of the multi-channel pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.7 Removing the upper part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.8 Installing the upper part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
 Table of contents
4/72 Eppendorf Reference® 2
English (EN)

7 Alignment/adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1 Adjusting the pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1.1 General information on user adjustment and the factory adjustment . . . . . . . . . . . . . 31
7.1.2 Changing the user adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.1.3 Changing the factory adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
7.1.4 Reference 2 - Changing the factory adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
7.2 Changing the volume for changed user adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.2.1 Changing volume for single-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.2.2 Volume change for multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

8 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
9.1 Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
9.1.1 Single-channel pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
9.1.2 Multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
9.2 Check after repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
9.2.1 Checks overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
9.2.2 Perform checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
9.3 Shipping the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

10 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
10.1 Increments of the single-channel and multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . 45
10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
10.3 Single-channel pipette with fixed volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
10.4 Single-channel pipette with variable volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
10.5 Multi-channel pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10.6 Errors of measurement in accordance with ISO 8655-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
10.7 Test conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
11.1 Single-channel lower parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
11.1.1 Ejector sleeves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
11.1.2 Piston, piston spring and piston mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
11.1.3 Protective filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
11.2 Multi-channel lower parts with fixed cone spacing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
11.2.1 O-rings and replacement channels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
11.3 Spare parts for single-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
11.3.1 Ejector sleeves for single-channel pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
11.3.2 Additional spare parts for single-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
11.4 Spare parts for multi-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
11.5 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
11.6 Service spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
11.6.1 Adjustment plug . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
11.6.2 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
11.6.3 Stroke system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
11.6.4 Counter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
11.6.5 Housing case, multi-channel lower part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
11.6.6 Housing lid, multi-channel lower part . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
11.6.7 O-ring tip cone, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
 Table of contents
Eppendorf Reference® 2 5/72
English (EN)

11.6.8 Ejector spring, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

11.6.9 Service tools stroke system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11.6.10 Sealing washers piston . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

12 Technical information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
 Table of contents
6/72 Eppendorf Reference® 2
English (EN)
 Operating instructions
Eppendorf Reference® 2 7/72
English (EN)

1 Operating instructions
1.1 Using this manual

 Make sure that the service manual and the operating manual are available in the latest versions. To do
so, compare the version numbers. The latest versions of the manuals can be downloaded from
www.eppendorf-support.com.
 Read the service manual before commencing work on the device.
 Read the chapters "Installation" and "Operation" of the operating manual.
 Observe the safety instructions in the operating manual.

1.2 Danger symbols and danger levels

The safety precautions in these instructions have the following danger symbols and danger levels.

1.2.1 Danger symbols

Biohazard Toxic substances

Hazard point Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning
1. Actions in the specified order
2.
 Actions without a specified order
• List
Direction of movement
Text Display or software texts
Additional information
 Operating instructions
8/72 Eppendorf Reference® 2
English (EN)

1.4 Purpose and scope

1.4.1 Purpose

This document describes all prerequisites and actions necessary to perform installation, diagnosis, repair
or maintenance of the product named on the title page.

1.4.2 Scope

This document applies to all service organizations of Eppendorf AG, service providers commissioned by
Eppendorf AG, as well as certified Eppendorf AG service technicians.

1.5 Version overview

Version Date of issue Chapter Change
03 2020-07 1, 9 Version history and function test supplemented.
 Product description
Eppendorf Reference® 2 9/72
English (EN)

2 Product description
2.1 Product overview
Abb. 2-1: Single-channel pipette and multi-channel pipette

1
2
3
4
17

Reference 2
5 AD
J
Reference 2

AD
J
6
7

000
0000

000
000
0

8
9
10 14
18

15
11 19
16
12

13

Fig. 2-1: Single-channel pipette and multi-channel pipette

1 Volume display 11 Ejector sleeve

2 Color code 12 Tip cone
3 Control button 13 Pipette tip
4 Volume catch 14 Lever
5 Single-channel upper part with nominal 15 Multi-channel lower part
volume
16 Switch spring load (On/Off)
6 User adjustment
17 Multi-channel upper part with nominal volume
7 User adjustment display
18 Housing lid
8 Serial number
19 Latch
9 RFID chip
10 Single-channel lower part with nominal
volume
 Product description
10/72 Eppendorf Reference® 2
English (EN)

2.2 Features

The Reference 2 pipette is a piston-stroke pipette for aspirating and dispensing liquids. The pipette
operates according to the air cushion principle. A suitable pipette tip must be fitted prior to use. The
dispensing, volume setting ring and ejector are controlled with the control button. Depending on the
model, volumes between 0.1 μL and 10 mL can be dispensed.

2.2.1 Pipette models

Different variants are available:

• Single-channel pipettes with fixed volume (fix)
• Single-channel pipettes with variable volume setting
• Multi-channel pipettes with 8 or 12 channels and variable volume setting
 Safety
Eppendorf Reference® 2 11/72
English (EN)

3 Safety
3.1 User profile

The specialist entrusted with maintenance, repair or testing must meet the following prerequisites:
• Successful participation in service trainings with certification by Eppendorf AG for the product that is to
be maintained, repaired or tested.
• Qualification as a specialist with knowledge of the applicable local and international standards.
• Qualification to evaluate the work assigned to him.
• Recognition of potential hazards and how to prevent or correct them.

3.2 Information on liability

The Eppendorf service organization responsible for servicing, repair, or testing is liable for ensuring that all
work is performed in a professional manner.

 The constructive condition of the device must not be altered as a result of repair, servicing, or testing.
The safety of the original condition must be maintained.
 Only accessories and original spare parts recommended by Eppendorf as well as test and measuring
equipment recommended by Eppendorf may be used for servicing, repair, and testing.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations,
the biosafety level of your laboratory, and the manufacturers' Safety Data Sheets and
application notes.
 Wear your personal protective equipment.
 For comprehensive regulations about handling germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health
Organization, Laboratory Biosafety Manual, in the currently valid version).

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.

 Wear your personal protective equipment.

 Observe the national regulations for handling these substances.
 Observe the material safety data sheets and manufacturer's application notes.

WARNING! Infection by contaminated material

There may be contaminated material on the device and the accessories. You may become
infected by contaminated material.

 Find out more about the risk of contamination before starting work.
 Check the device decontamination certificate of the device.
 Work on decontaminated devices only.
 Wear your personal protective equipment (protective gloves, protective goggles).
 Safety
12/72 Eppendorf Reference® 2
English (EN)

CAUTION! Poor safety due to incorrect accessories and spare parts

The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of accessories and spare parts other
than those recommended or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

 Operation
Eppendorf Reference® 2 13/72
English (EN)

4 Operation
4.1 Setting the volume

Set the volume from a high to a low value. If necessary, exceed the desired volume and then
go back.
1. Keep the volume catch pressed.
2. Turn the control button.
0 0 0 0
-

4.2 Attaching the pipette tips

The pipette tip can be attached by hand or mounted directly from a tip holder (tray) with the pipette.

The pipette tips are single-use items.

The control button and the trays are color-coded. The color marks the pipette size and the
volume of the pipette tip (epT.I.P.S.).
1. Insert the tip cone into the pipette tip using light
force.
 Operation
14/72 Eppendorf Reference® 2
English (EN)

4.3 Optimum immersion depths

Volume Immersion depth
0.1 μL – 1 μL 1 mm
1 μL – 100 μL 2 mm – 3 mm
100 μL – 1000 μL 2 mm – 4 mm
1 mL – 10 mL 3 mm – 5 mm

4.4 Forward pipetting

4.4.1 Aspirating liquid

Prerequisites
• The pipette tip has been attached.

To ensure maximum precision and accuracy, we recommend initially wetting each new tip by
aspirating and dispensing the liquid one to three times.

1. Press down the control button to the first stop.

2. Immerse the pipette tip vertically in the liquid.
3. Maintain the immersion depth and let the control button slide back slowly.
Liquid is aspirated into the pipette tip.
4. Wait until the liquid has been aspirated.
5. Remove the pipette tip from the liquid.

If necessary, wipe the pipette tip against the tube inner wall.

4.4.2 Dispensing liquid

1. Place the pipette tip on the tube inner wall at a steep angle.
2. Press down the control button to the first stop.
The liquid is dispensed.
3. Wait until the flow of liquid stops.
4. Press down the control button to the second stop.
The pipette tip is completely emptied.
5. Hold down the control button and wipe the pipette tip on the tube inner wall.

4.4.3 Ejecting the pipette tip

 Push the operating lever all the way down.

The pipette tip is ejected.
 Operation
Eppendorf Reference® 2 15/72
English (EN)

4.5 Reverse pipetting

An additional volume (blow out) is aspirated when performing reverse pipetting. This can improve
dispensing results for viscous or foaming liquids. Volume limits may occur when using filter tips.

4.5.1 Aspirating liquid

1. Press down the control button to the second stop.

2. Immerse the pipette tip vertically in the liquid.
3. Maintain the immersion depth and let the control button slide back slowly.
Liquid is aspirated into the pipette tip.
4. Wait approx. 3 seconds.
5. Remove the pipette tip from the liquid.

If necessary, wipe the pipette tip against the tube inner wall.

4.5.2 Dispensing liquid

1. Place the pipette tip on the tube inner wall at a steep angle.
2. Slowly press down the control button to the first stop.
The liquid is dispensed.
3. Wait until the flow of liquid stops.
4. Hold down the control button and wipe the pipette tip on the tube inner wall.
Residual liquid remains in the pipette tip.
During dispensing, the additional volume (blow out) does not belong to the dispensing volume.

4.5.3 Ejecting the pipette tip

1. Press down the control button to the second stop.

The residual liquid is dispensed.
The residual liquid can be discarded.
2. Press the control button down all the way.
The pipette tip is ejected.

4.6 Storing the pipette

The pipette can be stored in a pipette carousel, a wall mount or in horizontal position.
 Operation
16/72 Eppendorf Reference® 2
English (EN)
 Troubleshooting
Eppendorf Reference® 2 17/72
English (EN)

5 Troubleshooting
5.1 Control button

Problem Cause Solution

Control button The lower part is contaminated. Clean and grease the lower part.
jams.
The seal is defective. Replace seal.
The protection filter is blocked. Replace the protection filter (2 mL –
10 mL).

5.2 Dispensing

Problem Cause Solution

Liquid drips. Pipette tip is loose.  Firmly attach the pipette tip.
 Deactivate the spring-loaded action.
 Use the epT.I.P.S. pipette tip.
The seal is defective. Replace seal.
O-ring defective. Replace o-ring.
Dispensed liquid has a high vapor Pre-wet the pipette tip several times.
pressure.
The tip cone is damaged.  Replace the lower part
(single-channel pipette).
 Replace the channel (multi-channel
pipette).
Incorrect Dispensed liquid has a high vapor Adjust the pipette for the liquid used.
dispensing volume. pressure or a different density.

5.3 Pipette tip

Problem Cause Solution

Pipette tip is loose. The pipette tip is not compatible.  Use epT.I.P.S. pipette tips.
 Use a suitable size.
Higher attachment forces are required.  Firmly attach the pipette tip.
 Deactivate the spring-loaded action.
 Troubleshooting
18/72 Eppendorf Reference® 2
English (EN)

5.4 Tip cone

Problem Cause Solution

No spring-loaded The spring-loaded action is blocked.  Remove the locking ring
action of the tip (single-channel).
cone.  Remove the locking clip
(multi-channel).
Spring loading is switched off Switch on spring loading.
(multi-channel).
 Disassembly/assembly
Eppendorf Reference® 2 19/72
English (EN)

6 Disassembly/assembly
6.1 Disassembling the single-channel pipette lower part up to 1000 μL

Please complete the following steps in the order described:

Made in Made in Made in

Germany Germany Germany

PUSH TO RELEASE

1 2 3 4

1. Hold the control button completely pressed down.

2. Remove the ejector sleeve 1 .
3. 2 and 3 : On the lower part, push the ring marked "PUSH UP TO RELEASE" up by about 5 mm until the
lower part comes off.
4. 4 : Remove the lower part from the upper part.
1. Gently press together the stop pins at the piston mount (1).
1 2. Remove the piston mount.
3. Remove the piston (2) and the piston spring (3). The piston is
under spring tension.
2

4
 Disassembly/assembly
20/72 Eppendorf Reference® 2
English (EN)

6.2 Assembling the single-channel pipette lower part up to 1000 μL

Please complete the following steps in the order described:

1. Carefully guide the piston and the piston spring into the cylinder.
1 Make sure that the piston is guided correctly in the piston spring
and the cylinder. There must not be any perceptible resistance.
Stop pushing as soon as any resistance is felt. This may indicate
2
that the piston is not positioned correctly in the cylinder. There is
a risk of bending the piston if too much force is applied. Carefully
pull out the piston and repeat the process correctly. In the case of
piston springs with double windings (4), these windings must
3 point downwards.
2. Keep the piston and the piston spring pressed.
3. Keep the stop pins on the piston mount pressed with the other
4 hand.
4. Mount the piston mount so that the two locking mechanisms
engage in their holders.

5. Gently press a pipette tip against the inserted piston. The piston
must move down in the cylinder without any noticeable
resistance.
6. Insert the pre-assembled lower part of the pipette into the upper
part until it audibly engages.
7. Place the ejector sleeve on the ejector rod. If correctly fitted, it
will engage slightly.
 Disassembly/assembly
Eppendorf Reference® 2 21/72
English (EN)

6.3 Disassembling the lower part of the single-channel pipette from 2 mL

upward

Please complete the following steps in the order described:

Referen
Referen

ADJ ADJ

0000
000
0000
000 1

1 2 3

1. 1 Unscrew the ejector sleeve.

2. 2 : Keep the control button fully pressed and turn the lower part approximately 30° to the right.
3. 3 : Open the lower part: fit the pipette wrench 4 (included in the delivery package) onto the lower part.

Hold the cylinder and unscrew it from the lower part.

The lower part consists of the following:

1 2 3 4 5 6

1 2 mL – 10 mL piston mount 4 Piston (with seal)

2 Piston spring 5 Cylinder and tip cone
3 Upper part of the lower part 6 Filter sleeve with protection filter in the tip
cone
 Disassembly/assembly
22/72 Eppendorf Reference® 2
English (EN)

6.4 Assembling the lower part of the single-channel pipette from 2 mL

upward

1. Insert piston mount, piston spring and piston into the upper part of the lower part.

The further assembly corresponds to the figures indicated in chapter "Disassembling the lower part of the
single-channel pipette from 2 mL upward". Carry out the procedure in reverse order.

6.5 Disassembling the lower part of the multi-channel pipette

Abb. 6-1: Reference 2 Lower part 8-channel 100 μL

10
3
11 12

9
4
8
5

6
7

Fig. 6-1: Reference 2 Lower part 8-channel 100 μL

1 Piston mount 7 O-ring for tip cone

2 Piston spring 8 Latch
3 Piston attachment 9 Housing case
4 Piston with sealing 10 Spring ejector rail
5 Spring 11 Ejector rail
6 Tip cone 12 Housing lid
 Disassembly/assembly
Eppendorf Reference® 2 23/72
English (EN)

Please complete the following steps in the order described:

1 2 3

1. To unlock the lower part, slide the lever to the left or right.Remove the lower part of the pipette. (fig. 1 +
2)
2. Hold the lower part in such a way that the lever is facing downwards.
3. Slide the left and right latches upward. (Fig. 3)
4. Remove the housing lid with integrated ejector rail.

1 2 3 4 5

Remove the piston from the piston attachment (fig. 1 + 2) and remove the tip cone from the housing case
(fig. 3 - 5)

1. Carefully remove the piston from the piston attachment.

The piston attachment is in its highest position. Do not tilt the piston when removing it. The
piston may break.
2. Press the tip cone into the housing case until the spring is completely compressed. Tilt the tip cone and
remove it with spring and o-ring.

Remove all tip cones to continue with the disassembly of the housing case.

3. Press the piston mount and turn it 90° to the left or right. Remove the piston mount.

The piston mount is under slight spring pressure.

4. Remove the piston spring.

5. Slide the piston attachment downward. Slide the left and right latches upward.
6. Remove the latch of the housing case.
7. Remove the piston attachment from the housing case.
 Disassembly/assembly
24/72 Eppendorf Reference® 2
English (EN)

6.6 Assemble the lower part of the multi-channel pipette

Abb. 6-2: Reference 2 Lower part 8-channel 100 μL

10
3
11 12

9
4
8
5

6
7

Fig. 6-2: Reference 2 Lower part 8-channel 100 μL

1 Piston mount 7 O-ring for tip cone

2 Piston spring 8 Latch
3 Piston attachment 9 Housing case
4 Piston with sealing 10 Spring ejector rail
5 Spring 11 Ejector rail
6 Tip cone 12 Housing lid
 Disassembly/assembly
Eppendorf Reference® 2 25/72
English (EN)

Please complete the following steps in the order described:

1. Insert the piston attachment into the housing case.

2. Slide the piston attachment upward.

Check whether the piston attachment can be moved easily.

3. Push up the piston attachment. Assemble the piston spring and fix it onto the piston mount.
4. Insert all tip cones
5. Insert all pistons.

First, insert the piston in the tip cone. Then insert the piston in the piston attachment.

6. Attach the housing lid. To fix the housing lid, insert the right and left latch and push them up for
locking.

6.7 Removing the upper part

Gravimetric testing is required after opening the upper part of the Reference 2.

Prerequisites
The pipette lower part has been removed.

Required tools

• Flat head screwdriver size 5

• Disassembly tool

1. Press against the housing and press the control

button all the way to the lowest stop.
2. Remove the housing lid toward the top.
 Disassembly/assembly
26/72 Eppendorf Reference® 2
English (EN)

3. Remove the volume catch toward the top.

Observe the return springs while doing so.

4. Lift the counter with the tip of the flat head

screwdriver. Begin on the side displayed in the
image.
5. Changes sides, pry out the counter and remove it.

Do not apply the screwdriver beneath the bevel gear wheel.

6. Remove the carrier toward the top.

 Disassembly/assembly
Eppendorf Reference® 2 27/72
English (EN)

7. Select the lower spring and remove it.

Do not bend the holder of the stroke system. Risk of breakage.

8. Insert the disassembly tool in the pipette and

press the stroke system out.
The flat side of the disassembly tool faces the flat
side of the housing.
9. Remove the stroke system toward the top.
 Disassembly/assembly
28/72 Eppendorf Reference® 2
English (EN)

6.8 Installing the upper part

Required tools

• Assembly tool

1. Set the user adjustment to 0. Rotate the number

ring until the gear wheel is above the 0.

2. Insert the stroke system into the pipette housing

from above.
3. Position the fixing tool as shown in the image.
4. Press the stroke system down to engage is in the
fixing tool.
5. Check that the numbers of the user adjustment
are clearly visible in the display.

6. Insert the carrier.

 Disassembly/assembly
Eppendorf Reference® 2 29/72
English (EN)

7. Insert the counter.

8. Insert the pegs in the guide holes of the pipette
housing.
9. Press the counter down until it engages.

10. Insert the volume catch.

11. Put the lid over the front lug.

12. Push the lid down on the other side until it
engages with the rear lug.

13. Insert the spring in the lower opening of the

pipette and fasten it by inserting the lower part.
 Disassembly/assembly
30/72 Eppendorf Reference® 2
English (EN)

Gravimetric testing is required after opening the upper part of the Reference 2.
 Alignment/adjustment
Eppendorf Reference® 2 31/72
English (EN)

7 Alignment/adjustment
7.1 Adjusting the pipette
NOTICE! Incorrect dispensing volume with special liquids and due to temperature
differences.
Solutions with physical data very different to that of water or temperature differences between
the pipette, pipette tip and liquid may result in incorrect dispensing volumes.

 Avoid temperature differences between the pipette, pipette tip and liquid.

The random and systematic errors recorded upon delivery can be found on the Eppendorf
Certificate. This certificate is included in the delivery. If the factory adjustment is changed, the
certificate becomes invalid.

7.1.1 General information on user adjustment and the factory adjustment

The Reference 2 was adjusted and checked before delivery. The adjustment opening is sealed with a gray
plastic adjustment seal. The adjustment seal bears the abbreviation "ADJ". The adjustment display shows
"0".

Changing the adjustment of the Reference 2 is recommended for solutions whose density, viscosity, surface
tension and/or vapor pressure differs greatly from that of water. If the density of an aqueous solution
changes by approx. ±10 %, for example due to its salt concentration, its volume changes by approx.
±10 %. This does not apply if other relevant properties of the liquid also change.

If the location the pipette is used at is at an extreme altitude, correction due to atmospheric pressure is
required. At 1 000 m above sea level, a volume error of approx. -0.3 % occurs for a 100 μL pipette.

If special tips with shapes different from standard tips are used, changing the adjustment can improve
dispensing accuracy (systematic bias).

Observe the recommendations for adjusting epT.I.P.S. long in the User adjustment on the Reference 2 CD.

A changed adjustment can be cancelled in a few simple steps.

Aside from changes to the user adjustment, a Reference 2 can be changed permanently by changing the
factory adjustment.

Changes to the user adjustment or to the factory adjustment have no influence on dispensing precision
(random error). Precision can be improved by replacing worn parts. Precision is also greatly influenced by
the handling of the device.

Before changing the adjustment of factory calibration, check the existing dispensing rate.
 Alignment/adjustment
32/72 Eppendorf Reference® 2
English (EN)

You can determine the actual volume by weighing:

At 20 °C, the density of distilled water is approx. 0.9982 mg/μL, and 0.9965 mg/μL at 27 °C.

No correction is required if the set volume equals the actual volume.

If there is a difference between the actual volume and the set volume of distilled water, please check the
following:

• Is liquid dripping from the tip?

• Is the pipette tip leaking?
• Is the tip cone damaged?
• Are the piston and cylinder leaking?
Sufficient tightness is ensured if no drops from at the pipette tip after aspirating the nominal volume of
distilled water and a wait time of approx. 15 s. Hold the pipette vertically and do not touch the pipette
tip during this. For nominal volumes of ≤ 20 μL, pre-wet the tip multiple times.
• Is the temperature of the pipetted liquid the same as the:
– Temperature of the device?
– Temperature of the ambient air?
• Is the measuring place draft-free?
• Does the operation and the pipetting speed ensure complete aspiration and dispensing of the liquid?
• Has the correct numerical value for "Dense liquid at weighing temperature" been used to calculate the
actual volume?
• Is the set volume correct?
• For very small volumes (< 10 μL): Is the analytical balance sensitive enough (Scale resolution:
0.001 mg)?
• Have original epT.I.P.S pipette tips been used as test tips?
The pipette tip used as a test tip can be found in the technical data (see p. 47).

Only if all questions can be answered with "Yes" can adjustment be performed. In any other case, the
problems corresponding to the questions answered with "No" must first be remedied. If replacing the
complete lower part or other volume-determining parts, proper assembly must be gravimetrically checked.
The systematic and random errors to be fulfilled can be found in the technical data.
 Alignment/adjustment
Eppendorf Reference® 2 33/72
English (EN)

7.1.2 Changing the user adjustment

If the adjustment is changed, the volume is changed by a specific value. Strictly speaking, this change only
applies for the testing volume.

Example:

You are adjusting a 10 – 100 μL pipette by 1 μL at a volume setting of 100 μL (1 μL ≙ 1 %). The pipette is
also adjusted by 1 μL at a volume setting of 10 μL (≙ 10 %).

Auxiliary equipment
• Pin
• Gray adjustment seal (ADJ) made of plastic
1. Stab into the center of the gray adjustment seal (ADJ) with the pin.
2. Remove the adjustment seal.
3. Insert the adjustment tool.
4. Rotate the adjustment tool until the adjustment display shows the desired value.
5. Read the set value without distortion via the bearing aid in the viewing window.
6. Weight to check accuracy and precision.

1000 µL

ADJ

1
- 1
+

7. After the checks, insert the gray adjustment seal.

If the adjustment only applies for a specific liquid, mark the pipette correspondingly. Use the labeling area
on the pipette to note the liquid and the volume. Gravimetrically check any change to the adjustment.
Observe the test specifications in EN ISO 8655-2 and 8655-6. A Standard Operation Procedure (SOP) and
more information on user adjustment and factory adjustment can be found on the CD or our website
www.eppendorf.com.
 Alignment/adjustment
34/72 Eppendorf Reference® 2
English (EN)

7.1.3 Changing the factory adjustment

Auxiliary equipment
• Gray safety plug made of plastic
• Pin

Changes to the factory adjustment can be made with

the appropriate accessory parts.

The factory adjustment is marked via a safety plug.

The color of the safety plug indicates who applied it:
• Gray-Eppendorf AG
• Red - User
• Dark gray - Service

AD
J
Refe

The random and systematic errors recorded upon delivery can be found on the Eppendorf Certificate. This
certificate is included in the delivery. If the factory adjustment is changed, the certificate becomes invalid.

If the dispensing accuracy is only changed temporarily, changing the user adjustment is the correct
method. Before changing the user adjustment or the factory adjustment, observe the general information
provided (see General information on user adjustment and the factory adjustment on p. 31) and the
corresponding gravimetric checks.

7.1.4 Reference 2 - Changing the factory adjustment

The volume display is set to correspond to the piston stroke and the detected actual volume during factory
adjustment. If a deviation to be corrected is detected during the gravimetric check and factory adjustment
is required, proceed as follows:

1. Check that the adjustment display is set to "0".

If the adjustment display is not set to "0", it must
first be set to "0" with the adjustment tool. In this
case, do not proceed with changing the factory
adjustment, but check the Reference 2
gravimetrically with the adjustment display set to
1000 µL
"0".

ADJ

1
- 1
+
 Alignment/adjustment
Eppendorf Reference® 2 35/72
English (EN)

2. Pierce the safety plug with the pin and remove it.

AD
J
Refe

3. Insert the adjustment tool.

AD
J
Re

4. Hold the control button with one hand.

5. Rotate the adjustment tool to set the volume
display. Set the volume display from the volume
setting used in the check to the volume
determined via the gravimetric check.
The volume change applies for the entire volume
range. When changing the volume, first set the
value determined during the gravimetric check
AD
J for 10 % of the nominal volume. Then check
50 % and 100 % of the nominal volume
Refere

gravimetrically with this setting. If required,

change the selected setting again to achieve
optimal correction for all volumes. Determine
whether the data results are suitable with the
measurement deviation limit values in
accordance with ISO 8655-2 and the technical
data of Eppendorf AG.

6. Check the changes made gravimetrically.

 Alignment/adjustment
36/72 Eppendorf Reference® 2
English (EN)

7. Insert the gray safety plug in the adjustment

opening.
The pipette is identified as adjusted and
calibrated by the user via the dark gray safety
plug.

AD
J
Refere

8. Document all changes and measurements made.

 Alignment/adjustment
Eppendorf Reference® 2 37/72
English (EN)

7.2 Changing the volume for changed user adjustment

The volume values (Δ V) are theoretical values and used only for orientation. They apply for any volume set
for pipettes with variable volume setting. Deviations to the values may result depending on the operation
and other conditions (temperature, density, etc.).

7.2.1 Changing volume for single-channel pipettes

Nominal volume Color code User adjustment Δ V [μL]

+8 +6 +4 +2 -2 -4 -6 -8
1 μL dark gray 0.05 0.0375 0.025 0.0125 -0.012 -0.025 -0.037 -0.05
2 μL 5 5

2.5 μL
5 μL medium gray 0.2 0.15 0.1 0.05 -0.05 -0.1 -0.15 -0.2
10 μL
20 μL light gray 0.4 0.3 0.2 0.1 -0.1 -0.2 -0.3 -0.4
10 μL yellow 0.4 0.3 0.2 0.1 -0.1 -0.2 -0.3 -0.4
20 μL
25 μL yellow 2 1,5 1 0.5 -0.5 -1 -1.5 -2
50 μL
100 μL
200 μL yellow 4 3 2 1 -1 -2 -3 -4
300 μL orange 6 4.5 3 1.5 -1.5 -3 -4.5 -6
200 μL blue 20 15 10 5 -5 -10 -15 -20
250 μL
500 μL
1000 μL
2 mL red 50 37.5 25 12.5 -12.5 -25 -37.5 -50
2.5 mL
5 mL violet 100 75 50 25 -25 -50 -75 -100
10 mL turquoise 200 150 100 50 -50 -100 -150 -200
 Alignment/adjustment
38/72 Eppendorf Reference® 2
English (EN)

7.2.2 Volume change for multi-channel pipettes

Nominal volume Color code User adjustment Δ V [μL]

+8 +6 +4 +2 -2 -4 -6 -8
10 μL medium gray 0.2 0.15 0.1 0.05 -0.05 -0.1 -0.15 -0.2
100 μL yellow 2 1.5 1 0.5 -0.5 -1 -1.5 -2
300 μL orange 6 4.5 3 1.5 -1.5 -3 -4.5 -6
 Diagrams
Eppendorf Reference® 2 39/72
English (EN)

8 Diagrams

k
an
bl
ft
le
lly
na
io
nt
te
In
 Diagrams
40/72 Eppendorf Reference® 2
English (EN)
 Maintenance
Eppendorf Reference® 2 41/72
English (EN)

9 Maintenance
9.1 Service

For instructions on how to disassemble and assemble the pipette, read the corresponding
chapter "Disassembly/assembly".

Gravimetrically check the pipette if volume-determining parts have been replaced.

9.1.1 Single-channel pipettes

1. Check if the lower part is contaminated. Replace contaminated lower parts.

2. Check if the inserted lower part is bent. Replace bent lower parts.
3. Clean the tip cone using an alcohol-based solution. Inspect the tip seating for scratch marks. Replace
scratched lower parts.
4. Use the pipette tester to check if the pipette is leaky. Replace the piston with seal if necessary, then
repeat the test.
5. Check if the piston is broken or scratched. Replace broken or scratched pistons.
6. Clean the piston using an alcohol-based solution, then lubricate the piston.
7. Assemble the pipette. Set the nominal volume. Check to see if the piston runs smoothly and evenly by
slowly pressing the control button several times.

9.1.2 Multi-channel pipettes

1. Remove the tip cone O-rings using the multi-channel tool.

If the O-rings cannot be cut, the tool is worn and must be replaced.

2. Check if the tip cones are scratched. Replace scratched tip cones.
3. Place the new O-rings on the tip cones.
4. Check the piston along with the seal. Replace defective pistons with seals.
5. Clean the piston using an alcohol-based solution, then lubricate the piston.
6. Use the pipette tester to check each channel for leakage.
7. Assemble the pipette. Set the nominal volume. Check to see if the piston runs smoothly and evenly by
slowly pressing the control button several times.
 Maintenance
42/72 Eppendorf Reference® 2
English (EN)

9.2 Check after repair

9.2.1 Checks overview

Checks Single-channel, fixed Single-channel, Multi-channel

variable
Check customer adjustment X X X
Check piston stroke X X X
Check counter – X X
Replace o-rings of the tip cones – – X
Check volume dispensing X X X

9.2.2 Perform checks

Check Procedure Criterion/result

Check customer adjustment Visually check settings. Customer adjustment is set to 0.
Check piston stroke  Set the nominal volume. • No jerking movement can be
 Press the control button and felt.
let it slide back up again. • The piston does not stick.
• The control button is attached
firmly and does not wiggle.
Check counter Set the entire volume range. • The counter is OK.
• The numbers are in one row.
• The number output is OK.
O-rings of the tip cones Replace all O-rings. New o-rings have been fitted.
Check volume dispensing  Perform a calibration. The measurement results are
 Perform a gravimetric check in within the required limits.
accordance with the SOP.
 Maintenance
Eppendorf Reference® 2 43/72
English (EN)

9.3 Shipping the device

WARNING! Risk to health from contaminated device.

1. Observe the information in the decontamination certificate. It is available as a PDF
document on our webpage (www.eppendorf.com/decontamination).
2. Decontaminate all the parts to be shipped.
3. Include the fully completed decontamination certificate in the shipment.

NOTICE! Risk of damage due to incorrect packing.

Eppendorf AG is not liable for any damage caused by improper packing.

 Only store and transport the device in its original packing.

Transport packing can be ordered via your Eppendorf service organization. The order number
can be found in the chapter Ordering information.
 Maintenance
44/72 Eppendorf Reference® 2
English (EN)
 Technical data
Eppendorf Reference® 2 45/72
English (EN)

10 Technical data
10.1 Increments of the single-channel and multi-channel pipettes
Model Increment
0.1 μL – 2.5 μL 0.002 μL
0.5 μL – 10 μL 0.01 μL
2 μL – 20 μL 0.02 μL
2 μL – 20 μL 0.02 μL
10 μL – 100 μL 0.1 μL
20 μL – 200 μL 0.2 μL
30 μL – 300 μL 0.2 μL
100 μL – 1000 μL 1 μL
0.25 mL – 2.5 mL 0.002 mL
0.5 mL – 5 mL 0.005 mL
1 mL – 10 mL 0.01 mL

10.2 Ambient conditions

Temperature range Relative humidity
Storage without transport -5 °C — 45 °C 10 % — 95 %
package
Operating conditions 5 °C — 40 °C 10 % — 95 %
 Technical data
46/72 Eppendorf Reference® 2
English (EN)

10.3 Single-channel pipette with fixed volume

Model Test tip Measurement error
epT.I.P.S. Systematic Random
±% ± μL ±% ± μL
1 μL 0.1 μL – 10 μL 2.5 0.025 1.8 0.018
dark blue dark blue
2 μL 34 mm 2.0 0.04 1.2 0.024
dark blue
5 μL 0.1 μL – 20 μL 1.2 0.06 0.6 0.03
blue blue
10 μL 40 mm 1.0 0.1 0.5 0.05
blue
20 μL 0.5 μL – 20 μL L 0.8 0.16 0.3 0.06
light blue light blue
46 mm
10 μL 2 μL – 200 μL 1.2 0.12 0.6 0.06
yellow yellow
20 μL 53 mm 1.0 0.2 0.3 0.06
yellow
25 μL 1.0 0.25 0.3 0.075
yellow
50 μL 0.7 0.35 0.3 0.15
yellow
100 μL 0.6 0.6 0.2 0.2
yellow
200 μL 0.6 1.2 0.2 0.4
yellow
200 μL 50 μL – 1000 μL 0.6 1.2 0.2 0.4
blue blue
250 μL 71 mm 0.6 1.5 0.2 0.5
blue
500 μL 0.6 3.0 0.2 1.0
blue
1000 μL 0.6 6.0 0.2 2.0
blue
2.0 mL 0.5 mL – 2.5 mL 0.6 12 0.2 4
red red
2.5 mL 115 mm 0.6 15 0.2 5
red
 Technical data
Eppendorf Reference® 2 47/72
English (EN)

10.4 Single-channel pipette with variable volume

Model Test tip Testing Measurement error
epT.I.P.S. volume Systematic Random
±% ± μL ±% ± μL
0.1 μL – 2.5 μL 0.1 μL – 10 μL 0.1 μL 48.0 0.048 12.0 0.012
dark gray dark gray 0.25 μL 12.0 0.03 6.0 0.015
34 mm
1.25 μL 2.5 0.031 1.5 0.019
2.5 μL 1.4 0.035 0.7 0.018
0.5 μL – 10 μL 0.1 μL – 20 μL 0.5 μL 8.0 0.04 5.0 0.025
medium gray medium gray 1 μL 2.5 0.025 1.8 0.018
40 mm
5 μL 1.5 0.075 0.8 0.04
10 μL 1.0 0.10 0.4 0.04
2 μL – 20 μL 0.5 μL – 20 μL L 2 μL 3.0 0.06 1.5 0.03
light gray light gray 10 μL 1.0 0.10 0.6 0.06
46 mm
20 μL 0.8 0.16 0.3 0.06
2 μL – 20 μL 2 μL – 200 μL 2 μL 5.0 0.10 1.5 0.03
yellow yellow 10 μL 1.2 0.12 0.6 0.06
53 mm
20 μL 1.0 0.2 0.3 0.06
10 μL – 100 μL 2 μL – 200 μL 10 μL 3.0 0.3 0.7 0.07
yellow yellow 50 μL 1.0 0.5 0.3 0.15
53 mm
100 μL 0.8 0.8 0.2 0.2
20 μL – 200 μL 2 μL – 200 μL 20 μL 2.5 0.5 0.7 0.14
yellow yellow 100 μL 1.0 1.0 0.3 0.3
53 mm
200 μL 0.6 1.2 0.2 0.4
30 μL – 300 μL 20 μL – 300 μL 30 μL 2.5 0.75 0.7 0.21
orange orange 150 μL 1.0 1.5 0.3 0.45
55 mm
300 μL 0.6 1.8 0.2 0.6
100 μL – 1000 μL 50 μL – 1000 μL 100 μL 3.0 3.0 0.6 0.6
blue blue 500 μL 1.0 5.0 0.2 1.0
71 mm
1000 μL 0.6 6.0 0.2 2.0
0.25 mL – 2.5 mL 0.25 mL – 2.5 mL 0.25 mL 4.8 12 1.2 3
red red 1.25 mL 0.8 10 0.2 2.5
115 mm
2.5 mL 0.6 15 0.2 5
0.5 mL – 5 mL 0.1 mL – 5 mL 0.5 mL 2.4 12 0.6 3
violet violet 2.5 mL 1.2 30 0.25 6.25
120 mm
5.0 mL 0.6 30 0.15 7.5
1 mL – 10 mL 1 mL – 10 mL 1.0 mL 3.0 30 0.6 6
turquoise turquoise 5.0 mL 0.8 40 0.2 10
165 mm
10.0 mL 0.6 60 0.15 15
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10.5 Multi-channel pipette

Model Test tip Testing Measurement error
epT.I.P.S. volume Systematic Random
±% ± μL ±% ± μL
0.5 μL – 10 μL 0.1 μL – 20 μL 0.5 μL 12.0 0.06 8.0 0.04
medium gray medium gray 1 μL 8.0 0.08 5.0 0.05
40 mm
5 μL 4.0 0.2 2.0 0.1
10 μL 2.0 0.2 1.0 0.1
10 μL – 100 μL 2 μL – 200 μL 10 μL 3.0 0.3 2.0 0.2
yellow yellow 50 μL 1.0 0.5 0.8 0.4
53 mm
100 μL 0.8 0.8 0.3 0.3
30 μL – 300 μL 20 μL – 300 μL 30 μL 3.0 0.9 1.0 0.3
orange orange 150 μL 1.0 1.5 0.5 0.75
55 mm
300 μL 0.6 1.8 0.3 0.9
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10.6 Errors of measurement in accordance with ISO 8655-2

Nominal volume Maximum permissible errors (ISO 8655)
systematic random
±% ± μL ±% ± μL
1 μL 5.0 0.05 5.0 0.05
2 μL 4.0 0.08 2.0 0.04
5 μL 2.5 0.125 1.5 0.075
10 μL 1.2 0.12 0.8 0.08
20 μL 1.0 0.2 0.5 0.1
50 μL 1.0 0.5 0.4 0.2
100 μL 0.8 0.8 0.3 0.3
200 μL 0.8 1.6 0.3 0.6
500 μL 0.8 4.0 0.3 1.5
1000 μL 0.8 8.0 0.3 3.0
2000 μL 0.8 16 0.3 6.0
5000 μL 0.8 40 0.3 15.0
10000 μL 0.6 60 0.3 30.0

10.7 Test conditions

Test conditions and test evaluation in compliance with ISO 8655, Part 6. Tested using a standardized
analytical balance with evaporation protection.

The three largest testing volumes per tip (10 %, 50 %, 100 % of the nominal volume)
correspond to the specifications in accordance with ISO 8655, Part 2 or Part 5. The test is to
be carried out with these three testing volumes for testing of the systematic and random error
in compliance with the standard. The smallest adjustable volume serves to provide additional
information.
• Number of determinations per volume: 10
• Water according to ISO 3696
• Inspection at 20 °C – 27 °C
Maximum temperature variation during measurement ±0.5 °C
• Dispensing onto the tube inner wall
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11 Ordering information
11.1 Single-channel lower parts
Abb. 11-1: Lower parts for single-channel pipettes

2
4

Fig. 11-1: Lower parts for single-channel pipettes

1 Single-channel lower part 3 Single-channel lower part

1 μL – 1000 μL 2.5 mL – 10 mL
2 Mechanical coupling

Order no. Description

(International)
Single-channel lower part
Reference 2, Xplorer, Xplorer plus; with separate serial number
4920 601.001 2.5 μL, dark gray, variable: 0.1 μL - 2.5 μL, fixed: 1 μL, 2 μL
4920 602.008 10 μL, medium gray, variable: 0.5 μL - 10 μL, fixed: 5 μL, 10 μL
4920 603.004 20 μL, light gray, variable: 2 μL - 20 μL, fixed: 20 μL
4920 604.000 20 μL, yellow, variable: 2 μL - 20 μL, fixed: 10 μL, 20 μL
4920 605.007 100 μL, yellow, variable: 10 - 100 μL, fixed: 25 μL, 50 μL, 100 μL
4920 606.003 200 μL, yellow, variable: 20 - 200 μL, fixed: 200 μL
4920 607.000 300 μL, orange, variable: 30 - 300 μL
4920 608.006 1000 μL, blue, variable: 100 - 1000 μL, fixed: 200 μL, 250 μL, 500 μL, 1000 μL
4920 609.002 2.5 mL, red, variable: 0.25 - 2.5 mL, fixed: 2 mL, 2.5 mL
4920 610.000 5 mL, violet, variable: 0.5 - 5 mL
4920 611.007 10 mL, turquoise, variable: 1 - 10 mL
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11.1.1 Ejector sleeves

Abb. 11-2: Ejector sleeves for single-channel pipettes

1 2

Fig. 11-2: Ejector sleeves for single-channel pipettes

1 Ejector sleeve 2 Ejector sleeve

1 μL – 1000 μL 2.5 mL – 10 mL

Order no. Description

(International)
Ejector sleeve
Eppendorf Reference 2
4921 601.005 1 μL, color code: dark gray
4921 602.001 2 μL, color code: dark gray
4921 603.008 5 μL, color code: medium gray
4921 604.004 25 μL, color code: yellow
4921 605.000 50 μL, color code: yellow
4921 606.007 200 μL, color code: blue
4921 607.003 250 μL, color code: blue
4921 608.000 500 μL, color code: blue
4921 609.006 2 mL, color code: red
Ejector sleeve
Reference 2
4920 621.002 10 mL, color code: turquoise
4920 613.000 10 μL, color code: medium gray or yellow
4920 614.006 20 μL, color code: light gray or yellow
4920 615.002 100 μL, color code: yellow
4920 617.005 300 μL, color code : orange
4920 616.009 200 μL, color code: yellow
4920 618.001 1000 μL, color code: blue
4920 619.008 2.5 mL, color code: red
4920 620.006 5 mL, color code: violet
4920 612.003 2.5 μL, color code: dark gray
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11.1.2 Piston, piston spring and piston mount

Abb. 11-3: Example graphic for the volume range 1 μL – 1000 μL

2
1 4

Fig. 11-3: Example graphic for the volume range 1 μL – 1000 μL

1 Piston mount 3 Piston spring

2 Piston

Order no. Description

(International)
Piston
3120 602.003 2.5 μL, color code: dark gray; variable: 0.1 μL - 2.5 μL
3120 603.000 10 μL, color code: medium gray; variable: 0.5 μL - 10 μL, fixed: 10 μL
3120 604.006 20 μL, color code: light gray or yellow; variable: 2 μL - 20 μL, fixed: 10 μL - 20 μL
Piston with seal
3120 605.002 100 μL, color code: yellow; variable: 10 - 100 μL, fixed: 25 μL, 50 μL, 100 μL
3120 606.009 200 μL, color code: yellow; variable: 20 - 200 μL, fixed: 200 μL
3120 607.005 300 μL, color code: orange; variable: 30 - 300 μL
Piston with seal and spring
3120 608.001 1000 μL, color code: blue; variable: 100 - 1000 μL, fixed: 200 μL, 250 μL, 500 μL,
1000 μL
Piston spring
3120 609.008 2.5 μL, 10 μL, 20 μL
3120 610.006 100 μL
3120 611.002 200 μL
3120 612.009 300 μL
Piston mount
3120 601.007 2.5 μL - 1000 μL
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11.1.3 Protective filter

Abb. 11-4: Filter and accessories

5
1 2 3

Fig. 11-4: Filter and accessories

1 Protective filter 4 Removal tool for 5 mL filter sleeve

2 Filter sleeve 4 Removal tool for 10 mL filter sleeve
3 Removal tool for 2.5 mL filter sleeve

Order no. Description

(International)
Protection filter 2.5 mL
10 filters with 1 sleeve
4920 622.009 0.25 – 2.5 mL variable, 2 mL fixed volume, 2.5 mL fixed volume, color code red, 1
pipette wrench
Protection filter 5 mL
10 filters with 1 sleeve
4920 623.005 0.5 – 5 mL variable, color code violet, 1 pipette wrench
Protection filter 10 mL
10 filters with 1 sleeve
4920 624.001 1 – 10 mL variable, color code turquoise, 1 set = 1 pipette wrench
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11.2 Multi-channel lower parts with fixed cone spacing

Abb. 11-5: Multi-channel lower parts with fixed cone spacing

2 3

9 mm

Fig. 11-5: Multi-channel lower parts with fixed cone spacing

1 Mechanical coupling 3 12-channel lower part

9 mm cone spacing for 96-well plates
2 8-channel lower part
9 mm cone spacing for 96-well plates

Order no. Description

(International)
Eppendorf Reference 2 lower part
8-channel; lower part with separate serial number
4922 601.009 0.5 – 10 μL, medium gray
4922 603.001 10 – 100 μL, yellow
4922 605.004 30 – 300 μL, orange
Eppendorf Reference 2 lower part
12-channel; lower part with separate serial number
4922 602.005 0.5 – 10 μL, medium gray
4922 604.008 10 – 100 μL, yellow
4922 606.000 30 – 300 μL, orange
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11.2.1 O-rings and replacement channels

Abb. 11-6: Replacement channel

3
4
5

Fig. 11-6: Replacement channel

1 Piston 4 Tip cone

2 Cylinder 5 O-ring
3 Piston spring

Order no. Description

(International)
Replacement channel for multichannel lower parts
includes piston, cylinder, tip spring
4922 607.007 10 μL, color code: medium gray, for multichannel 0.5 - 10 μL
3122 607.002 10 μL, color code: medium gray, for multichannel 0.5 – 10 μL
Replacement channel for multichannel lower parts
includes piston, cylinder, tip spring, O-ring
4922 608.003 100 μL, color code: yellow, for multichannel 10 - 100 μL
4922 609.000 300 μL, color code: orange, for multichannel 30 - 300 μL
3122 608.009 100 μL, color code: yellow, for multichannel 10 - 100 μL
3122 609.005 300 μL, color code: orange, for multichannel 30 - 300 μL
O-ring
Red, for multichannel lower parts
3122 611.000 5 - 100 μL, 15 - 300 μL (1 set = 24 pieces)
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11.3 Spare parts for single-channel pipettes

11.3.1 Ejector sleeves for single-channel pipettes
Abb. 11-7: Example: 100 μL and 5 mL ejector sleeve

Fig. 11-7: Example: 100 μL and 5 mL ejector sleeve

Order no. Description

(International)
Ejector sleeve
Reference 2
4920 612.003 2.5 μL, color code: dark gray
4920 617.005 300 μL, color code : orange
4920 620.006 5 mL, color code: violet
4920 621.002 10 mL, color code: turquoise
4920 613.000 10 μL, color code: medium gray or yellow
4920 614.006 20 μL, color code: light gray or yellow
4920 615.002 100 μL, color code: yellow
4920 616.009 200 μL, color code: yellow
4920 618.001 1000 μL, color code: blue
4920 619.008 2.5 mL, color code: red
Ejector sleeve
Eppendorf Reference 2
4921 601.005 1 μL, color code: dark gray
4921 602.001 2 μL, color code: dark gray
4921 603.008 5 μL, color code: medium gray
4921 604.004 25 μL, color code: yellow
4921 605.000 50 μL, color code: yellow
4921 606.007 200 μL, color code: blue
4921 607.003 250 μL, color code: blue
4921 608.000 500 μL, color code: blue
4921 609.006 2 mL, color code: red
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11.3.2 Additional spare parts for single-channel pipettes

Abb. 11-8: Examples for the 1 – 1000 μL volume range

1 3

Fig. 11-8: Examples for the 1 – 1000 μL volume range

1 Piston mount 3 Piston spring

2 Piston

Order no. Description

(International)
Piston
3120 602.003 2.5 μL, color code: dark gray; variable: 0.1 μL - 2.5 μL
3120 603.000 10 μL, color code: medium gray; variable: 0.5 μL - 10 μL, fixed: 10 μL
3120 604.006 20 μL, color code: light gray or yellow; variable: 2 μL - 20 μL, fixed: 10 μL - 20 μL
Piston with seal
3120 605.002 100 μL, color code: yellow; variable: 10 - 100 μL, fixed: 25 μL, 50 μL, 100 μL
3120 606.009 200 μL, color code: yellow; variable: 20 - 200 μL, fixed: 200 μL
3120 607.005 300 μL, color code: orange; variable: 30 - 300 μL
Piston with seal and spring
3120 608.001 1000 μL, color code: blue; variable: 100 - 1000 μL, fixed: 200 μL, 250 μL, 500 μL,
1000 μL
Piston spring
3120 609.008 2.5 μL, 10 μL, 20 μL
3120 610.006 100 μL
3120 611.002 200 μL
3120 612.009 300 μL
Piston mount
3120 601.007 2.5 μL - 1000 μL
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11.4 Spare parts for multi-channel pipettes

Abb. 11-9: Example: Replacement channel for 10 μL, 100 μL und 300 μL multi-channel lower part

Fig. 11-9: Example: Replacement channel for 10 μL, 100 μL und 300 μL multi-channel lower part

Order no. Description

(International)
Replacement channel for multichannel lower parts
includes piston, cylinder, tip spring
4922 607.007 10 μL, color code: medium gray, for multichannel 0.5 - 10 μL
Replacement channel for multichannel lower parts
includes piston, cylinder, tip spring, O-ring
4922 608.003 100 μL, color code: yellow, for multichannel 10 - 100 μL
4922 609.000 300 μL, color code: orange, for multichannel 30 - 300 μL
O-ring
Red, for multichannel lower parts
3122 611.000 5 - 100 μL, 15 - 300 μL (1 set = 24 pieces)
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11.5 Tools
Abb. 11-10: IMAGE CAPTION ??? Tools for single- and multi-channel pipettes

1 2 3 4 5 6

Fig. 11-10: Tools for single- and multi-channel pipettes

1 Pipette wrench 4 Adjustment tool

For 2 – 10 mL single-channel lower parts For setting pipetting volume (e.g., for liquids with
high densities or different ambient conditions)
2 Multi-channel tool | Multi-channel cutting tool
For multi-channel lower parts 5 Pin
For removing the adjustment seal
3 Unlocking tool
For multi-channel lower parts | For 1200 μL 6 Safety plug tool
multi-channel lower part

Order no. Description

(International)
Pipette wrench 2-10 mL
Research plus, Reference 2, Xplorer, Xplorer plus
3120 634.002
Locking ring
to prevent any spring effect of the tip cones with single-channel pipettes
3120 635.009 10 – 1000 μL
Multi-channel tool
for cutting the O-rings on the tip cone of multi-channel lower parts
3122 610.003 5 - 100 μL or 15 - 300 μL
Adjustment tool
3120 633.006 for changing the factory adjustment or user adjustment
Safety plug tools
5 pieces, for changing the factory adjustment
3120 639.004 Incl. pin for loosening the safety plug
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11.6 Service spare parts

1
6

1 Stroke system 4 Volume catch

2 Adjustment seal 5 Counter
3 Lid 6 Gray safety plug (plastic)

11.6.1 Adjustment plug

ADJ

1 2

1 Adjustment seal 2 Gray safety plug (plastic)

Order no. Description

(International)
Adjustment seal
gray
4920 801.000 20 pieces
Plug
gray
4920 802.007 20 pieces
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11.6.2 Lid

Order no. Description

(International)
Lid
variable
4920 803.003 2,5 μL
4920 806.002 100 μL
Lid
fixed
4921 801.004 1 μL
4921 810.003 250 μL
4921 811.000 500 μL
4921 812.006 1 mL
4921 813.002 2 mL
4921 814.009 2,5mL
Volume catch
4920 829.002
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11.6.3 Stroke system

0 +1 +2

Order no. Description

(International)
Stroke system
variable
4920 813.009 2.5 μL
4920 814.005 10 μL
4920 815.001 20 μL, grey
4920 816.008 20 μL, yellow
4920 817.004 100 μL
4920 818.000 200 μL
4920 819.007 300 μL
4920 820.005 1 mL
4920 821.001 2,5 mL
4920 822.008 5 mL
10 mL
Stroke system
fixed
4921 815.005 1 μL
4921 816.001 5 μL
4921 817.008 10 μL, yellow
4921 818.004 20 μL, grey
4921 819.000 200 μL, blue
4921 820.009 2 mL
Stroke system
multi-channel
4922 801.008 10 μL
4922 802.004 100 μL
4922 803.000 300 μL
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11.6.4 Counter

Order no. Description

(International)
Counter
4920 824.000 0-2-4
4920 825.007 0-1-2
4920 826.003 0-5-0

11.6.5 Housing case, multi-channel lower part

Order no. Description

(International)
Housing case
8-channel
4922 804.007 10 μL
4922 806.000 100 μL
4922 808.002 300 μL
Housing case
12-channel
4922 805.003 10 μL
4922 807.006 100 μL
4922 809.009 300 μL
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11.6.6 Housing lid, multi-channel lower part

Order no. Description

(International)
Housing lid
8-channel
4922 810.007 10 μL
4922 812.000 100 μL
4922 814.002 300 μL
Housing lid
12-channel
4922 811.003 10 μL
4922 813.006 100 μL
4922 815.009 300 μL

11.6.7 O-ring tip cone, multi-channel

1 O-ring

Order no. Description

(International)
O-ring
Red, for multichannel lower parts
0013 021.733 5 - 100 μL, 15 - 300 μL, 2,5 mm × 1 mm (1 piece)
3122 611.000 5 - 100 μL, 15 - 300 μL (1 set = 24 pieces)
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11.6.8 Ejector spring, multi-channel

1 Spring for ejector 2 Ejector

Order no. Description

(International)
Ejector spring
Reference 2, Research plus, Xplorer, Xplorer plus
0013 021.810 10 pieces
Ejector
4922 816.005 10 μL, 8-Kanal
4922 818.008 100 μL - 300 μL, 8-Kanal
4922 817.001 10 μL, 12-Kanal
4922 819.004 100 μL - 300 μL, 12-Kanal
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11.6.9 Service tools stroke system

1 2

1 Disassembly tool 2 Fixing tool

Order no. Description

(International)
Fixing tool
4920 827.000
Disassembly tool
4920 828.006
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11.6.10 Sealing washers piston

1 Sealing washer

Order no. Description

(International)
Sealing ring
Reference 2, Research plus, Xplorer, Xplorer plus
3120 046.020 100 μL, 24 pieces
3122 056.022 300 μL, 24 pieces
Sealing ring
single-channel
3120 056.025 200 μL
3120 066.047 1000 μL
4920 099.020 2,5 mL
3120 076.026 5 mL
3120 086.021 10 mL
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12 Technical information
 Technical information
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