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INTERNATIONAL ISO
STANDARD 22718
Second edition
2015-12-01
AMENDMENT 1
2022-08
Cosmetics — Microbiology —
Detection of Staphylococcus aureus
AMENDMENT 1
Cosmétiques — Microbiologie — Détection de Staphylococcus aureus
AMENDEMENT 1
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ISO 22718:2015/Amd.1:2022(E)
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ISO 22718:2015/Amd.1:2022(E)
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ISO 22718:2015/Amd.1:2022(E)
Foreword
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accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
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ISO 22718:2015/Amd.1:2022(E)
Cosmetics — Microbiology — Detection of Staphylococcus
aureus
AMENDMENT 1
5.3.3.1.1
Add the following text at the end of the paragraph:
Modified Eugon LT broth can be used as an alternative.
5.3.3.1.3
Add the following new subclause (5.3.3.2) as an alternative to Eugon LT 100 liquid broth:
5.3.3.2 Modified Eugon LT broth
5.3.3.2.1 Composition
Pancreatic digest of casein 15 g
Papaic digest of soybean meal 5g
Sodium chloride 4g
L-cystine 0,7 g
Sodium sulphite 0,2 g
Glucose 5,5 g
Egg lecithin 1g
Polysorbate 80 15 g
Sodium lauryl ether sulfate 1,56 g
Water 1 000 ml
5.3.3.2.2 Preparation
Dissolve successively into boiling water polysorbate 80 and egg lecithin until they are completely
dissolved. Dissolve the other components by mixing while heating. Dispense the medium into
suitable containers. Sterilize in the autoclave at 121 °C for 15 min. Mix well after sterilization while
the liquid is still hot to redissolve settled substances. After sterilization, the pH shall be equivalent
to 7,0 ± 0,2 when measured at room temperature.
5.3.3.2
Renumber the former subclause 5.3.3.2 to 5.3.3.3:
5.3.3.3 Other enrichment broths
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ISO 22718:2015/Amd.1:2022(E)
ICS 07.100.40
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