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Osmosis Lab

1) The purpose of the lab was to determine how different concentrations of starch solutions are affected by passive transport through a semi-permeable dialysis membrane when placed in water. 2) It was hypothesized that osmosis would occur, causing water to diffuse into the dialysis bag containing starch solutions to make the solutions isotonic, decreasing the bag's mass. 3) Dialysis bags containing various starch solutions were placed in beakers of water with iodine. The initial and final masses of each bag were recorded and the mass changes analyzed.

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138 views3 pages

Osmosis Lab

1) The purpose of the lab was to determine how different concentrations of starch solutions are affected by passive transport through a semi-permeable dialysis membrane when placed in water. 2) It was hypothesized that osmosis would occur, causing water to diffuse into the dialysis bag containing starch solutions to make the solutions isotonic, decreasing the bag's mass. 3) Dialysis bags containing various starch solutions were placed in beakers of water with iodine. The initial and final masses of each bag were recorded and the mass changes analyzed.

Uploaded by

MutuluShakur
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© Attribution Non-Commercial (BY-NC)
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Diffusion & Osmosis Design Lab Purpose: To determine how starch solutions affect passive transport within a semi-permeable

dialysis membrane. Hypothesis: If a solution of starch is placed in a dialysis bag within a beaker of water then osmosis will occur and try to make the solution isotonic causing a decrease in the mass of water within the beaker. Materials: Procedure: 1) Obtain a dialysis tube that has been soaked in water and tie off one end using the string to form a bag. 2) Place the 2% starch solution into the bag using a funnel and tie off the other end tightly. 3) Record the initial mass of the bag using the electronic balance. 4) Clean and rinse the beaker thoroughly before filling it with 2/3 of distilled water. 5) Place 10 drops of iodine into the beaker. 6) Immerse the bag into the beaker of water. 7) After 30 minutes retrieve the bag from the beaker and record its final mass. 8) Record the difference between the initial and final masses in the observation table. 9) Clean counter using paper towels and clean and rinse beakers thoroughly for next solution. 10) Repeat steps 1-9 for the next 4 starch solutions. Observations: Paper Towel 5 500 ml/600ml Beakers Electronic Balance String 5 Dialysis Tubes Starch Solutions (1%, 2%, 3%, 4%, 5%) Distilled Water Funnel Iodine Dropper Bottle

Starch %

Mass of Dialysis tubing with starch concentration (g)

1%

1.5

Mass of Dialysis tubing after being placed in iodine solution(g) 1.7

Difference in Mass (g) (final valueinitial value) 1.7 - 1.5 = 0.2 4.4 - 4.5 = 0.1 4.8 - 3.5 = 1.3 3.6 - 4.7 = 1.1 5.5 - 5.4 = 0.1

Increase or Decrease in Mass Increased 0.2g Decreased 0.1g Increased 1.3g Decreased 1.1g Increased 0.1g

2% 3%

4.5 3.5

4.4 4.8

4% 5%

4.7 5.4

3.6 5.5

Resulting Colours of dialysis tube and iodine solution Tube: light blue Solution: light purple Tube: blue Solution: purple Tube: dark blue Solution: dark purple Tube: light blue Solution: purple Tube: blue Solution: purple

Graph:
6 5 4

Mass (g)

3 Initial Mass (g) 2 1 0 1 2 3 4 5 Final Mass (g)

Starch %

Conclusion: The hypothesis stated was proven to a certain point, by the active transport of the iodine solution from the beaker into the dialysis tube. This is caused by the high concentration of iodine in the solution moving into the dialysis tube. Osmosis is apparent as the colour of the starch solution changed to a bluish-purple.

Source of Errors: 1) It is not possible to stop all starch from leaking out, just from tying a string. 2) The scale used to measure the mass of the solution could not have been 100% accurate. The scale may have had little amounts of water left on it from previous measurements that could not have been wiped off. 3) The beakers used were not 100% clean. Previous remaining reactants from older labs may have mixed with the solutions of this lab, hence leading to wrong calculations. Discussion: i) The dialysis tubing is semi-permeable like a cell wall of an animal cell. ii) Both, an animal cell and the dialysis tubing are semi-permeable, the dialysis tubing only separates molecules by size but a semi-permeable membrane of an actual cell has other factors to look at. Classification of the electrical charge. iii) The independent variables for this lab were the starch solutions of 1%, 2%, 3%, 4%, 5% and the iodine. The dependant variables for this lab were the initial mass and final mass of the starch solutions in the dialysis tubing. The control in this lab was the distilled water. iv) The substance with the largest molecule was starch. The starch molecules being largest could not get out of the dialysis tubing because they did not fit. v) Quantitative evidence was visible in terms of change in mass, but water did not affect the qualitative results. vi) Iodine was used to observe osmosis. With iodine in the water it was easy to observe osmosis as the iodine would change the colour to a bluish-purple.

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