User’s Guide

PLC 2050/2250/2500 Purification Systems

Trademarks
All product and company names are trademarks™ or registered® trademarks of their
respective holders. Use of the trademark(s) in this document does not imply any
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 TABLE OF CONTENTS
CHAPTER 1 | SAFETY | 7
Symbols and Notices | 8

Chemical Hazards | 9

Electrical Hazards | 9

Mechanical Hazards | 9

Flammable Solvents | 10

Lifting | 10

Replacement Parts | 10

Signs of Damage | 10

Site Requirements | 10

Spacing | 10

Storage and Movement | 10

CHAPTER 1 | INTRODUCTION | 11
Description | 12

Unpacking | 14

Technical Specifications | 19

Detailed Description | 22

Terms and Abbreviations | 32

Customer Service | 32

CHAPTER 2 | INSTALLATION | 33
Place the Solvent Bottle Tray | 34

Plumbing Connections | 34

Column Setup | 40

Install the Racks | 42

Install the Stylus | 43

Make the Power Connection | 43

Install Aftermarket Options | 44

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 3

 CHAPTER 3 | OPERATION | 49
Start Up | 50

Prime the Pump | 51

Loop Injection with Automatic Valve | 52

TABLE OF CONTENTS

Loop Injection with Manual Valve | 53

Power Down | 54

CHAPTER 4 | MAINTENANCE | 55
Helpful Hints | 56

Cleaning and Decontamination | 56

Maintenance Schedule | 58

Replace a Pump Seal | 59

Clean or Replace a Pump Check Valve | 62

Clean the Pump Pistons | 64

Replace the Mixing Chamber Piston Seal | 65

Test and Repair the UV-VIS Detector | 66

CHAPTER 5 | TROUBLESHOOTING | 67
Troubleshooting Table | 68

Repair and Return Policies | 73

APPENDIX A | WARRANTY | 75
General | 75

Limited Warranty | 76

4 TABLE OF CONTENTS | PLC 2050/2250/2500 Purification Systems

APPENDIX B | REPLACEMENT PARTS AND ACCESSORIES | 77
Couplers and Adapters | 78

Detector Parts | 78

Fittings | 79

TABLE OF CONTENTS
Fraction Collector | 79

Miscellaneous | 80

Pump Parts | 81

Tubing | 83

Valves | 84

APPENDIX C | MATERIALS | 85
Liquid Contact Materials | 85

PLC WITH GX-241 LIQUID HANDLER

APPENDIX D |
CONFIGURATION | 89
Unpack the System | 90

System Setup | 90

Connect the Transfer Tubing | 90

Control Software | 90

Installation | 91

Plumbing Connections | 92

Transfer Tubing Connection | 94

Rear Panel Connections | 94

Start Up | 96

Operational Description of the VERITY 4020 Syringe Pump | 97

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 5

 APPENDIX E | PLC WITH VERITY 1920 MS CONFIGURATION | 99
Unpack the System | 100

Control Software | 100

System Setup | 100

TABLE OF CONTENTS

Electrical Connections | 100

Exhaust Connections | 101

Plumbing Connections | 102

Start Up | 104

APPENDIX F | PLC WITH INTEGRATED ELSD | 105

Technical Specifications | 106

Description | 107

Make the Rear Panel Connections | 108

Install the Nebulizer and Nebulization Chamber | 111

Front Panel Connections | 112

Install the Chamber Shield | 114

Operation | 114

Split Flow Rates and Ratios | 117

Maintenance | 118

Troubleshooting | 119

Clean the Nebulizer | 120

Decontaminate the Nebulizer | 121

6 TABLE OF CONTENTS | PLC 2050/2250/2500 Purification Systems

 Chapter 1 | SAFETY

IN THIS CHAPTER:

● Symbols and Notices | 8

● Chemical Hazards | 9
● Electrical Hazards | 9
● Mechanical Hazards | 9
● Flammable Solvents | 9
● Lifting | 10
● Replacement Parts | 10
● Signs of Damage | 10
● Site Requirements | 10
● Spacing | 10
● Storage and Movement | 10

Performing any task not listed in this user’s guide will result in loss of warranty and may be
WARNING
dangerous.

Read this chapter before installing and operating the instrument.

Only trained technical personnel in a laboratory environment may use the instrument for
non-medical, liquid handling purposes. For safe and correct use of the instrument, operating and
service personnel must follow all instructions contained in this guide when installing, cleaning,
and maintaining the instrument. All safety precautions must be observed during all phases of
operation, service, and repair of the instrument.
Failure to comply with these precautions or with warnings described in the user’s guide violates
safety standards of design, manufacture, and intended use of the instrument. Gilson assumes no
liability for customers failing to comply with these requirements.
The instrument has been certified to safety standards required in Canada, Europe, and the
United States. Refer to the rear panel label on the instrument and the Declaration of Conformity
document for the current standards to which the instrument has been found compliant.
Read all documentation and safety information for accessories, peripherals, and other instruments
that may be used with this instrument before operating the system.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 7

 Symbols and Notices
Electrical and Hazard Symbols
The following electrical and hazard symbols may appear on the instrument or in this document:
SAFETY

SYMBOL EXPLANATION

Direct Current

Alternating Current

Protective Conductor Terminal

| Electrical Power ON

O Electrical power OFF

Caution

Caution, Risk of Electric Shock

Caution, Ultraviolet Light, Risk of UV Radiation

Caution, Two Person Lift Required

Warning, Corrosive Chemical

Caution, Hot Surface

8 SAFETY | PLC 2050/2250/2500 Purification Systems

Safety Notices
The following safety notices may appear in this document:

WARNING Indicates a potentially hazardous situation which, if not avoided, may result in personal injury.

SAFETY
Indicates a potentially hazardous situation which, if not avoided, may result in minor or
CAUTION
moderate injury.

Indicates a potentially hazardous situation which, if not avoided, may result in equipment
NOTICE
damage.

Chemical Hazards
Any chemicals used for analysis should be handled according to good laboratory practice
(GLP). They should also be stored, used, and disposed of in accordance with the manufacturer’s
specifications, as well as local and national regulations. Potentially hazardous chemicals can be
used with the instrument. Use care when handling chemicals and wear appropriate personal
protective equipment (PPE), such as safety glasses, gloves, etc.
The responsible individual must ensure that personnel are not exposed to hazardous levels of toxic
substances as outlined in the Safety Data Sheets (SDSs), or any documentation provided by local
governing bodies such as The Health Protection Agency (United Kingdom) or The Occupational
Safety and Health Administration (United States).

Electrical Hazards
Unless specifically instructed, do not remove any protective covers. Detach all sources of voltage
from the instrument before the service, repair, or exchange of parts.
Use only the grounded AC cord provided. Ungrounded power cords can result in electrical shock
and serious personal injury. Faulty or frayed power cords must be immediately replaced with one
of the same type and rating. When it is necessary to use a non-original power cord, make sure the
replacement cord adheres to following specifications and local building safety codes: 1) European
Union Model; Connector A: Male, Type E or F (Schuko), 16A; Connector B: Female, IEC320/C13,
10A; 250 V~, H05VV-F 3G1.0 mm² and 2) United States and Canadian Model; Connector A: Male
Type, NEMA 15-5, 15A; Connector B: Female, IEC320/C13, 10A; 125 V~, SVT 3x18 AWG.

Use only approved fuses with the specified current rating. The PLC Purification System must be
NOTICE
operated within the voltage specified on the marking plate of the instrument.

Mechanical Hazards
The housing of the PLC Purification System must be attached permanently with all screws
WARNING tightened at 3 N m. Unless specifically instructed, do not remove any protective covers.
All protections must be in place before starting the PLC Purification System.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 9

 Flammable Solvents
Secure all flammable solvents.

Lifting
The PLC Purification System exceeds the weight one person can lift safely. Two or more people
SAFETY

are required to lift the PLC Purification System safely. Always lift the PLC Purification System
from the base and follow any unpacking instructions provided with the instrument. For more
information, see Unpacking on page 14.

Replacement Parts
Be sure to use only replacement parts mentioned in this user’s guide.
See Replacement Parts and Accessories on page 77.

Signs of Damage
Do not attempt to use the instrument if there are visible signs of damage.

Site Requirements
Do not operate the instrument if site conditions are not within specifications. Refer to
Technical Specifications on page 19 for more details.

Spacing
Allow sufficient spacing around the system for proper cooling and for the connection of power
cords, plumbing, injection pump, liquid handler, external detectors, or any other peripherals.

Storage and Movement

Run a clean solvent through the fluid path before storing the PLC Purification System
(see Cleaning and Decontamination on page 56. Do not leave buffer in the PLC Purification System,
as it may cause blockages and damage the seals. Rinse the fluid path and prevent the liquids
from flowing out inlets and outlets before moving the PLC Purification System.
Disconnect the PLC Purification System electrically, use the provided plugs for the inlets and
outlets, and keep away from collision and moisture in an environment that complies with the
Technical Specifications on page 19.
When moving the PLC Purification System, use a cart or moving device if it is possible.

10 SAFETY | PLC 2050/2250/2500 Purification Systems

 Chapter 1

Chapter 1 | INTRODUCTION

IN THIS CHAPTER:

● Description | 12
● Unpacking | 14
● Technical Specifications | 19
● Detailed Description | 22
● Terms and Abbreviations | 32
● Customer Service | 32

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 11

 Description
PLC 2050/2250/2500 Purification Systems are
designed for preparative liquid chromatography
(PLC) to aspirate mobile phase solvents, form
gradients, inject liquid samples, and provide
Description

solutions for a preparative column for high

performance liquid chromatography (HPLC),
centrifugal partition chromatography (CPC),
or counter-current chromatography (CCC)
applications. A built-in detector measures the
absorbance and sends the chromatogram to the
on-board control software, GLIDER Software.
Fractions are collected with the integrated fraction
collector.

Standard Components
The following diagrams provide a general
description of the PLC Purification System with
standard components.

Figure 1
PLC 2050 Purification System

Column and Clamp

Refer to page 40 for
installation details Solvent Bottle Tray
Refer to page 34 for
installation details

Touchscreen PC
Refer to page 30 for a
detailed description

Electronic Connections:
Analog, USB, and Collection Probe and
External Controls Collection Valve
Refer to page 31 for Refer to page 30 for
detailed descriptions detailed descriptions

Racks
Two-Way Gradient Valve
Refer to page 42 for
Refer to page 26 for a installation details
detailed description

Dynamic Mixing Chamber Prime and Purge Valve and Pump Heads
Refer to page 25 for a Refer to page 22 for detailed descriptions
detailed description

Figure 2
Standard Components for the PLC Purification System (Left and Front View)

12 Introduction | PLC 2050/2250/2500 Purification Systems

 Stylus
Refer to page 13
for installation details

USB Connections

Description
Refer to page 31 for a
detailed description

RS-232, LAN, Power Connection,

and Voltage Selector
Refer to page 31 for detailed
descriptions

Figure 3
Standard Components for the PLC Purification System (Right and Front View)

Optional Components
The following diagrams provide a general description of the PLC Purification System with optional
components.
Acquisition Module for
Backflush Valve Additional Detectors
Refer to page 28 for a (Not Shown)
detailed description Refer to page 45 for
installation details

Manual Column Switching

Valve
Refer to page 44 for
installation details
Fraction Collector Cover
Kit
Refer to page 46 for
Automatic OR Manual installation details
Loop Injection Valve
Refer to page 27 for
detailed descriptions Funnel Rack
(Not Shown)
Refer to page 47 for
Standalone Column
Holder (Not Shown) installation details
Refer to page 45 for
installation instructions
Rack Overlays
(Not Shown)
Four-Way Gradient Valve Refer to page 46 for
Refer to page 26 for a installation details
detailed description

Figure 4
Optional Components for the PLC Purification System (Left and Front View)

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 13

 GX-241 Liquid Handler Configuration
PLC Purification Systems can be configured with a GX-241 Liquid Handler and
VERITY® 4020 Single Syringe Pump for automated sample injections.

GX-241
Unpacking

The optional GX-241 is a compact X/Y/Z instrument designed for automating general liquid
handling procedures. For more information, refer to the GX-241 Liquid Handler User’s Guide.

VERITY 4020 SYRINGE PUMP

The required VERITY 4020 Syringe Pump is equipped with a user-selectable, small- or
large-capacity syringe, allowing for a wide range of liquid transfers up to 25 mL in a single stroke.
The syringe pump enables automatic, unattended injections on PLC Purification Systems, allowing
users to purify more compounds with less manual interaction. For more information, refer to the
VERITY® 4X20 Syringe Pumps User’s Guide.

VERITY® 1920 Mass Spectrometer Configuration

The optional VERITY 1920 MS is a complete, compact, and fully packaged solution
for mass spectrometry (MS) purification. For more information, refer to the
VERITY® 1920 Mass Spectrometer User’s Guide.

Other Configurations
PLC Purification Systems can be configured with an external injection pump for high volume
injections and additional detectors, such as an evaporative light scattering detector (ELSD). All
these optional devices can be controlled with GLIDER.

Unpacking
Upon receipt of the instrument, inspect the exterior of all shipping boxes. All boxes should arrive
unopened and undamaged. If examination reveals that damage has occurred in shipment, notify
the carrier and Gilson immediately. Refer to Customer Service on page 32.

WARNING
Do not plug in the PLC Purification System if any damage is detected. Powering the
PLC Purification System in a damaged state may result in serious injury and may damage
internal components of the PLC Purification System.

The PLC Purification Systems are delivered with major components already assembled. Keep the
original container and packing assembly so the unit may be shipped safely, if necessary. Carefully
unpack the PLC Purification System and its accessories. Verify that all parts are included and
undamaged. Do this immediately, even if the system will not be used right away. Many carriers
must receive concealed damage claims within seven days of delivery.

A PLC Purification System weighs approximately 65 kg (143 lbs.) and an additional 20 kg

(44 lbs.) with packaging. The PLC Purification System is too heavy to be lifted or moved by
CAUTION one person safely. To avoid personal injury and for general safety, if moving or lifting the
PLC Purification System, always get another person to assist you. Always follow local health
and safety regulations.
Wear appropriate personal protection equipment (PPE), such as safety shoes, when moving
the PLC Purification System.
Do not attempt to lift the PLC Purification System from a valve. Always grip it from its base.

14 Introduction | PLC 2050/2250/2500 Purification Systems

To unpack the instrument:

1. Open the box.

2. Remove the carton of accessories.
3. Remove the packing material.

Unpacking
4. Lift the unit out of the box and place it at suitable location, such as a lab bench or cart, and
always on a flat, level surface.

It is recommended to operate the PLC Purification System inside a fume hood to ensure
proper ventilation.
Allow sufficient spacing around the system for proper cooling and for the different
NOTICE connections.
Free spaces of at least 20 cm must be kept around the instrument at all times to allow
for proper tubing and cable connections and to provide adequate ventilation during
operation.

5. Verify that all components from the Unpacking List on page 15 are included.

Unpacking List
The following items are considered standard equipment and are provided with
the PLC Purification Systems. For detailed descriptions and part numbers, refer to
Replacement Parts and Accessories on page 77.

STANDARD EQUIPMENT

PLC 2050 Purification System and PLC 2250 Purification System

● Inlet/suction, 1/8" (OD), 2.4 mm (ID), 2 m (qty. 2)
○ PTFE with a 20 cm spring at one end and an SS ballast at the other, and with colored cable
ties for identification
○ PEEK nuts and ETFE ferrules for 1/8" tubing
● Waste outlet, 1/8" (OD), 1.6 mm (ID), 2 m (qty. 1)
○ ETFE with an SS ballast at one end
○ SS fitting and PEEK nut and ETFE ferrule for 1/8" tubing

PLC 2500 Purification System

● Inlet/suction, 3/16" (OD), 1/8" (ID), 2 m (qty. 2)
○ PFA with an SS ballast at one end with colored cable ties for identification
○ PEEK nuts and ETFE ferrules for 3/16" tubing
● Waste outlet, 1/8" (OD), 2.4 mm (ID), 2 m (qty. 1)
○ PTFE with an SS ballast at one end
○ SS fitting and PEEK nut and ETFE ferrule for 1/8" tubing

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 15

 All PLC Purification Systems
● Additional collection probes (qty. 2)
○ ETFE Luer Male-to-1/4"-28 (TPI) Male
○ SS probe for 150 mm tubes (6.5 cm)
● Fittings for pump cleaning discs connections (qty. 2)
Unpacking

○ PEEK nuts and ETFE ferrules for 1/8" tubing

● Tubing kit for column connections
○ SS tubing, 1/8" (OD), 2.1 mm (ID), 1 m, for column inlet/outlet (qty. 2)
○ SS tubing, 1/16" (OD), 1 mm (ID), 1 m, for column inlet/outlet (qty. 2)
○ ETFE tubing, 1/8" (OD), 1.6 mm (ID), 1 m, for column outlet (qty. 1)
○ PTFE tubing, 1/8" (OD), 2.4 mm (ID), 1 m, for column outlet (qty. 1)
○ PTFE tubing, 1/16" (OD), 1 mm (ID), 1 m, for column outlet (qty. 1)
○ SS fittings (nuts and ferrules) for 1/8" tubing (qty. 4)
○ SS fittings (nuts and ferrules) for 1/16" tubing (qty. 4)
○ Outlet reducer (SS adapter with nut and ferrule for 1/16" tubing) (qty. 2)
● Column clamp (qty. 1)
○ For columns up to 40 mm (OD)
○ 3 mm Allen wrench
● Detector test cell for service (qty. 1)
● Gradient valve to pump heads tubing assembly (qty. 1)
○ PTFE, 1/8" (OD), 2.4 mm (ID) with springs
○ PEEK nuts and ETFE ferrules for 1/8” tubing
○ Tee union
● Positioning guide for placing short racks (qty. 1)
● Power cord, based on destination country (qty. 1)
● Purge valve outlet, 1.5 m (qty. 1)
○ ETFE, 1/8” (OD), 1.6 mm (ID) with an SS ballast at one end
○ SS fitting and PEEK nut and ETFE ferrule for 1/8” tubing
● Racks (qty. 3)
○ SS, 18 x 150/80 mm for 18 mm diameter tubes
● Sample loop fittings, unless the sample loop is already supplied (qty. 2)
● Solvent bottle tray (qty. 1)
● Stylus (qty. 1)
○ Adhesive holder
○ M3 fixing screw
● USB drive (qty. 1)
○ Documentation
○ Control software installation file

16 Introduction | PLC 2050/2250/2500 Purification Systems

Documentation
● PLC 2050/2250/2500 Purification Systems User’s Guide
● GLIDER Software User’s Guide
● UV-VIS Detector Service Guide

Unpacking
OPTIONAL ACCESSORIES

Factory Installed
● Backflush valve, automatic (qty. 1)
○ 4-way, 2-position electric valve for 1/8” tubing, special screws, and cable
○ Control module, power supply, bracket, and cables
○ Tubing kit, 1 mm (ID) (PLC 2050) or 2.1 mm (ID) (PLC 2250 and PLC 2500)
● Column switching valve, automatic (qty. 1)
○ 6-way, 2-position electric valve for 1/8” tubing, special screws, and cable
○ Control module, power supply, bracket, and cables
○ Tubing kit 1 mm (ID) (PLC 2050) or 2.1 mm (ID) (PLC 2250 and PLC 2500)
● Integrated ELS Detector
○ SEDEX FP™ nebulizer
○ Glassware nebulization chamber
○ Chamber shield
○ Accessory kit:
○ Splitter tubing (PEEK), 1/16” (OD) with PEEK fittings, and PEEK union 10-32
► Gas tube (PA), 6mm (OD), 3 m
► Gas tube (PU), 4 mm (OD), 23 cm
► Drain tube assembly (including SS fitting and PTFE seal)
► 2 adjustable releasable clips for black exhaust tube
○ Gas regulator with 5 µm filter (ordered separately):
► Manometer
► Fittings
► Set of two side mounting brackets
● Loop injection valve, automatic (qty. 1)
○ 6-way, 2-position electric valve for 1/8” tubing, special screws, and cable
○ Control module, power supply, bracket, and cables
○ Tubing kit 1 mm (ID) (PLC 2050) or 2.1 mm (ID) (PLC 2250 and PLC 2500)
● Quaternary gradient system 1/8” (PLC 2050 and PLC 2250) or 3/16” (PLC 2500) (qty. 1)
○ 4-way gradient valve
○ Inlet/suction tubing with fittings (qty. 4)

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 17

 Aftermarket Options
● Acquisition module for external detectors (qty. 1)
○ USB cable (qty. 1)
○ USB drive with drivers and installation procedure (qty. 1)
● Loop injection valve, manual (qty. 1)
Unpacking

○ 6-way, 2-position manual valve for 1/8" tubing, and bracket

○ Tubing kit 1 mm (ID) (PLC 2050) or 2.1 mm (ID) (PLC 2250 and PLC 2500)
○ Hardware and installation procedure
○ Accessory kit
► Large-bore syringe needle (qty. 1)
► Needle port cleaner (qty. 1)
► Allen wrenches (qty. 2)
► Open-end spanner, 1/4”—5/16” (qty. 1)
► Operating instructions (qty. 1)
● Fraction collector cover kit (qty. 1)
○ Cover with hinges and transparent door
○ Outlet adaptor
○ Clamp ring
○ Installation procedure
● Funnel rack with holder, 16 outlets (qty. 1)
○ Tubing with fittings, PTFE, 1.5m, 1/4" (OD), 4.75 mm (ID) (qty. 16)
○ SS probe for Funnel Rack (5.3 cm)
○ Stickers for outlet tubes identification
○ Installation procedure
● Manual column switching valve (qty. 1)
○ 6-way, 2-position manual valve for 1/8" tubing and bracket
○ Tubing kit, 1 mm (ID) (PLC 2050) or 2.1 mm (ID) (PLC 2250 and PLC 2500)
● Standalone column holder for LC and flash columns (qty. 1)
○ ETFE or PTFE tubing kit 1/8” (OD)

18 Introduction | PLC 2050/2250/2500 Purification Systems

Technical Specifications
Please be aware of the following before operating the instrument.

Changes or modifications to the instrument not expressly approved by Gilson could void the
NOTICE
factory-authorized warranty.

Technical Specifications
This instrument complies with part 15 of the Federal Communications Commission (FCC) rules.
Operation is subject to the following two conditions: (1) this instrument may not cause harmful
interference, and (2) this instrument must accept any interference received, including interference
that may cause undesired operation.
Shielded cables must be used with the instrument to ensure compliance with the FCC Class A
limits.

PLC 2050/2250/2500 Purification Systems

SPECIFICATION DEFINITION

Collection Options Several rack options available. Refer to Fraction Collector on page 79 in the
Replacement Parts and Accessories appendix.
Rack overlays for easy tube identification (optional). Refer to
Install the Rack Overlays on page 46.
Funnel rack with 16 outlets for large and variable collection volume in external high-
capacity containers (optional). Refer to Install the Funnel Rack on page 47.
Fraction collector cover kit (optional).
Install the Fraction Collector Cover Kit on page 46.

Column Holder Built-in column clamp (standard). 40 mm maximum (OD) for columns. Refer to
Column Setup on page 40.
Additional standalone holder for flash columns. 400 mm maximum height. 115 mm
maximum diameter.

Contact Control Analog signal input: Two contacts +/- (-1 V 𝌂 ; and +1V 𝌂, use of
10‑bit A/D converter)
Two inputs (contact closure, CMOS open drain): Start In and Stop In
(5 V 𝌂 maximum)
Two outputs (static relay contacts): Start Out and Stop Out (5 V 𝌂 maximum)

Control Via integrated touchscreen PC with GLIDER Software. Refer to the

GLIDER Software User's Guide.

PLC 2050/2250/2500 Purification Systems TECHNICAL SPECIFICATIONS CONTINUED ON PAGE 20

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 19

 PLC 2050/2250/2500 Purification Systems
SPECIFICATION DEFINITION

Detection UV monochromator single wavelength detector, 254 nm by default (standard)

UV monochromator dual wavelength detector, 254/280 nm by default (optional)
Technical Specifications

UV detector, 4-wavelength DAD, 200–400 nm (optional)

UV-VIS detector, 4-wavelength DAD, 200–600 nm (optional)
UV-VIS detector, 4-wavelength DAD, 200–800 nm (optional)
VERITY 1920 MS (optional). Refer to the PLC with VERITY 1920 MS Configuration
appendix.
Standalone or built-in evaporative light scattering detector (ELSD) (optional). Refer
to the PLC with Integrated ELSD appendix.

Dimensions (W x D x H) 62 x 59 x 66 cm (24.4 x 23.2 x 26 in.)

Electrical Protection General: Delayed action fuses 6.3A H 250 V~, T-type (qty. 2)
24 V 𝌂 and 5 V 𝌂: Delayed action fuses with different ratings, L 250 V~, T-type

Environmental Conditions Specification Definition

Environment Indoor use only

Altitude Up to 2000 m

Temperature Range 5°C to 40°C (41°F to 104°F)

Humidity Maximum relative humidity 80% for temperatures

up to 31°C, decreasing linearly to 50% relative
humidity at 40°C

External Ports USB ports

Serial port RS-232 (COM4)
LAN port

Gradient Former Binary (standard)

Quaternary (optional)
Linear response from 2% to 98%

Injection Options Automatic loop injection valve (optional). Refer to

Loop Injection Valve Options on page 27.
Manual loop injection valve (optional). Refer to
Loop Injection Valve Options on page 27.
Additional injection pump (1–50 mL/min or 5–250 mL/min, optional).
Additional GX-241 Liquid Handler (optional). Refer to the PLC with Configuration
appendix.

PLC 2050/2250/2500 Purification Systems TECHNICAL SPECIFICATIONS CONTINUED ON PAGE 21

20 Introduction | PLC 2050/2250/2500 Purification Systems

 PLC 2050/2250/2500 Purification Systems
SPECIFICATION DEFINITION

Liquid Contact Materials* Description Material

*Refer to Materials on page 85 for Gradient valve (gradient former) and PEEK and Kalrez®

Technical Specifications
more information. collection valve

Shut-off valve for PTFE

automatic injection valve

Pressure transducer and seal AI2O3 and Kalrez®

Manual injection valve 316 SS and PEEK

Pump heads, check valve housings, 316L SS

purge valve holder, and ballasts

Mixing chamber 316L SS and PTFE

Pump piston seals GFP and Hastelloy C

Pump pistons Zirconium oxide

Pump check valves PCTFE, ruby, and sapphire

Purge valve Ketron® CA30 PEEK and Kalrez®

Tubing, fittings, sample loops, and 316/316L SS

unions PEEK, ETFE, PTFE, PFA

UV detector cell Fused silica, PTFE, and 316 SS

Racks Three racks, 18 x 150/180 mm tubes (long)

Several rack options (long or short) available. Refer to
Fraction Collector on page 79 in the Replacement Parts and Accessories
appendix for a complete listing.
Possible to combine different rack models.

Power Requirements Specification Description

Line Voltage 110–120 / 220–240 V~ (single-phase)

Line Frequency 50/60 Hz

Wattage 450 W maximum

Distribution TT or TN power system only

Transient overvoltages Category II
Class 1 equipment

PLC 2050/2250/2500 Purification Systems TECHNICAL SPECIFICATIONS CONTINUED ON PAGE 22

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 21

 PLC 2050/2250/2500 Purification Systems
SPECIFICATION DEFINITION

Pump PLC 2050 50 mL/min (1 to 50 mL/min)

Flow rate (minimum to maximum) 300 bar (4351 psi)
Detailed Description

Maximum pressure
PLC 2250 250 mL/min (5 to 250 mL/min)
230 bar (3336 psi)

PLC 2500 500 mL/min (10 to 500 mL/min)

110 bar (1595 psi)

Pump Flow Rate Accuracy: ±2% (with H 2O degassed at 20°C)

Performance Repeatability: Better than 0.5% RSD

Safety and Compliance The instrument has been certified to safety standards specified for Canada, Europe,
and the United States. Refer to the instrument rear panel label and the Declaration
of Conformity document for the current standards to which the instrument has
been found compliant.

Storage 450 GB of hard memory

Up to 32 methods in control software memory

Valve Options Automatic backflush valve (optional)

Manual column switching valve (optional)

Weight 65 kg (143 lbs.)

Detailed Description
Two-Head Pump and Purge Valve
The two-head pump can deliver mobile phases at flow rates up to 500 mL/min (depending
on model) with low residual pulsation. PLC Purification Systems utilize high performance, dual
piston, reciprocal pumps. The pump generates suction through a system of pulleys, belt, and a
stepper motor, which drives the cams and pistons. For plumbing connections, refer to the table
on page 24.

22 Introduction | PLC 2050/2250/2500 Purification Systems

A manual valve at the outlet (black knurled knob) allows users to easily purge or prime the pump;
the valve is associated with a pressure transducer, which monitors the pressure in the system.

Cleaning Purge
Belt Cam Discs Valve

Detailed Description
Pump
Heads
Pistons

Pulley

Motor

Figure 5 Figure 6
Interior View of Pump Pump Heads and Purge Valve

H J

P Q
Q

N
G

M M
C2
L L
C2
K
F
F

Z
Z
Z

D A
B
A
C1
A

Figure 7
Component and Connection Diagram for the Pump Heads and Prime/Purge Valve

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 23

 Pump and Purge Valve Components and Connections
COMPONENT DESCRIPTION COMPONENT DESCRIPTION

A PTFE tubing, 1/8" (OD), 2.4 mm (ID) J SS, preformed tubing, 1/8” (OD),
with springs (PN 21040137) for 1 mm (ID) (PN 21040121) for PLC 2050
Detailed Description

PLC 2050 and PLC 2250 SS, preformed tubing, 1/8” (OD),
PFA tubing, 3/16" (OD), 1/8" (ID) 2.1 mm (ID) (PN 21040117) for PLC 2250
(PN 21040139) for PLC 2500 and PLC 2500

B PEEK tee union, 1/4"-28, 2.4 mm K ETFE junction tubing for cleaning discs
bore (PN 21040201) for PLC 2050
and PLC 2250
PEEK Y assembly, 5/16"-24 to 10‑32
(PN 4957515) for PLC 2500

C1 PEEK nuts L Outlet check valve housings

Long for 1/8" tubing (PN 21040195 Check valves (PN 21040103)
for PLC 2050 and PLC 2250)
Standard for 3/16" tubing
(PN 49040132 for PLC 2500)

C2 PEEK short nuts for 1/8" tubing M SS nut for 1/8" tubing (PN 21040199)
(PN 21040194) SS ferrule for 1/8" tubing
(PN 21040200)

D ETFE ferrules N Outlet

1/8” tubing (PN 21040193) for
PLC 2050 and PLC 2250
3/16” tubing (PN 490410133N) for
PLC 2500

F Pump heads P Purge valve outlet (waste)

Purge valve outlet tubing
(PN 21040168)

G Prime and purge valve Q Pump head outlets

H SS, preformed tubing, 1/8” (OD), Z Inlet check valve housings

1 mm (ID) (PN 21040122) for Check valves (PN 21040103)
PLC 2050
SS, preformed tubing, 1/8” (OD),
2.1 mm (ID) (PN 21040118) for
PLC 2250 and PLC 2500

24 Introduction | PLC 2050/2250/2500 Purification Systems

Dynamic Mixing Chamber
Located at the outlet of the pump, the dynamic mixing
chamber utilizes a motor-driven magnetic agitator to
thoroughly mix solvents and ensure smooth gradients
at high flow rates.

Detailed Description
Mixing volume depends on pump model installed:
● 4 mL for a 50 mL/min pump (PLC 2050)
Figure 8
● 12 mL for a 250 mL/min pump (PLC 2250) Dynamic Mixing Chamber
● 16 mL for a 500 mL/min pump (PLC 2500)
Refer to the diagram below for components and connections to the dynamic mixing chamber.

B A
D

Figure 9
Component and Connection Diagram for the Mixing Chamber

Dynamic Mixing Chamber Components and Standard Connections

COMPONENT DESCRIPTION COMPONENT DESCRIPTION

A Prime and purge valve C SS, preformed tubing, 1/8” (OD), 1 mm

(ID) (PN 21040123) for PLC 2050
SS, preformed tubing, 1/8” (OD), 2.1 mm
(ID) (PN 21040119) for PLC 2250 and
PLC 2500

B Dynamic mixing chamber D SS nut for 1/8" tubing (PN 21040199)

SS ferrule for 1/8" tubing (PN 21040200)

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 25

 Inlet
Two- or Four-Way Gradient Valve A
Outlet

A two- (standard) or four-way (optional) low

pressure valve, located on the left side of the
instrument, permits users to perform binary or
quaternary elution gradients. The low pressure
Detailed Description

valve accommodates up to four solvents. The

binary gradient valve comes standard with
PLC 2050/2250/2500 Purification Systems. The
optional quaternary gradient valve (PN 21040001
Figure 10
for PLC 2050 and PLC 2250; PN 21040002 4-Way Gradient Valve
for PLC 2500) is also available. For plumbing
connections, refer to Gradient Valve to Pump Heads on page 37.

Binary and Quaternary Low Pressure Valves Service Parts

PART NUMBER BINARY VALVES PART NUMBER QUATERNARY VALVES

21040143 1/8" with cable, PLC 2050/2250 21040145 1/8" with cable, PLC 2050/2250

21040144 3/16" with cable, PLC 2500 21040175 3/16" with cable, PLC 2500

INLET TUBING
Inlet tubing connects the gradient valve to the solvent reservoirs. Use the two (standard) or
four (optional) inlet lines supplied. Each inlet line has a fitting and spring on one end and an
SS ballast on the other end.

Low
Pressure
Plug
Valve
Body

Spring
ETFE
Ferrule

PEEK
Nut

Figure 11
Inlet Tubing Connections

26 Introduction | PLC 2050/2250/2500 Purification Systems

Loop Injection Valve Options
AUTOMATIC LOOP INJECTION VALVE
The six-way automatic loop injection valve allows users to load a sample into the sample loop
while the system is running. The sample can be automatically injected into the column by
switching the valve via GLIDER.

Detailed Description
Status
Injection E
LEDs
Port D
F
J
H
C J
Sample
Loop
G 1
6 2

5 3
4
A

G G

Figure 12 Figure 13
Automatic Loop Injection Valve Component and Connection Diagram
for the Automatic Loop Injection Valve

Automatic Loop Injection Valve Components and Connections

COMPONENT DESCRIPTION COMPONENT DESCRIPTION

A SS, preformed tubing, 1/8” (OD), F Sample loop, preformed SS,

1 mm (ID) (PN 21040124) for PLC 2050 1/8" (OD), 2.1 mm (ID). Refer to
SS, preformed tubing, 1/8” (OD), Sample Loops on page 82 for
2.1 mm (ID) (PN 21040120) for PLC 2250 a complete listing.
and PLC 2500

B Dynamic mixing chamber G SS nut for 1/8" tubing

(PN 21040199)
SS ferrule for 1/8" tubing
(PN 21040200)

C Automatic loop injection valve H Shut-off valve, PLC, 1/8"

(PN 21040172

D Shut-off valve outlet tubing, 1/8" (OD), J PEEK short nuts for 1/8" tubing
1.6 mm (ID) (PN 21040169) (PN 21040194)
ETFE ferrules for 1/8” tubing
(PN 21040193)

E Injection port (PN 21040163)

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 27

 MANUAL LOOP INJECTION VALVE
Mounted on the left side of the system, the six-way manual loop injection valve allows users to
load a sample into the sample loop while the system is running. The sample can be manually
injected into the column by switching the valve.
Detailed Description

Sample
Loop

Injection
Port

Handle

Figure 14
Manual Loop Injection Valve

BACKFLUSH VALVE
The four-way automatic backflush valve allows users to change the pumping direction (elution
mode) into the connected column at any time while the system is running:
● Direct 'Dir' / Reverse 'Rev', for a HPLC column
● Ascending 'Asc' / Descending 'Dsc', for a CPC column

Outlet

To
Column

To
Inlet Column

Figure 15
Backflush Valve

28 Introduction | PLC 2050/2250/2500 Purification Systems

UV Detector
The standard UV detector is equipped with a deuterium lamp and monochromator to monitor
desired wavelength. The optional DAD (UV or UV-VIS) models monitor a large range of
wavelengths. With DAD, the control software can acquire signals issued from four different
wavelengths simultaneously. In addition, a continuous scan allows the acquisition of a full

Detailed Description
spectrum, which is displayed on the main screen.

UV AND UV-VIS DAD OPTIONS

● UV, monochromator, 254 nm by default (standard)
● UV, dual wavelength, 254/280 nm by default (optional)
● UV detector, four-wavelength; DAD, 200–400 nm (optional)
● UV-VIS detector, four-wavelength; DAD, 200–600 nm (optional)
● UV-VIS detector, 4-wavelength; DAD, 200–800 nm (optional)

Fraction Collector
After detection of the signal (chromatogram), the results obtained at the outlet of the column
(peaks) can be directed to tubes in racks. A three-way collection valve located on the dispense
arm allows users to direct fractions to collection tubes or to waste.

Dispense
Arm

Dispense Spiral Waste

Wrap Outlet Inlet
Head

Collection
Probe

Tubes

Racks Collection Dispense Collection

Valve Head Probe

Figure 16 Figure 17
Fraction Collector with Standard Racks 3-Way Collection Valve

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 29

 COLLECTION PROBES
Different models of collection probes can be connected to the dispense head of the fraction
collector. The installed probe is SS, 35 mm (PN 21040159). A second probe, SS, 65 mm is supplied
to collect fractions in 150 mm tubes. An additional probe is available, which is shorter, but with
a smaller outlet diameter for a fine and accurate dispense.
Detailed Description

Additional
Probe
Standard (ETFE
Dispense Luer
Probe Male-
to-1/4”-28
Male)

Figure 18
Collector Probes

Back Pressure Regulator

An in-line back pressure regulator 20 psi (1.4 bar) (PN 49041121) is mounted on the collector inlet
tubing to prevent air bubbles in the detector flow cell.

For the PLC 2500, the back pressure regulator is replaced by a simple union because it
creates too much back pressure at high flow rates. The back pressure regulator is supplied as
NOTICE an accessory.
You can install and use the back pressure regulator 20 psi on PLC 2500 if you are working
with flow rates less than 300 mL/min.

Racks
The integrated collector is provided with three
SS racks (standard, PN 21040019) designed for USB USB
18 mm diameter tubes, accommodating up to 192 Touchscreen Ports Ports
tubes and a maximum volume of 6.1 L. Refer to
Fraction Collector on page 79 for a complete list of
rack options.

Integrated Touchscreen PC
The PLC Purification System is controlled by
GLIDER Software that runs on an integrated
touchscreen PC, which allows the user to create
methods and run the PLC Purification System.
Seven USB ports are available, two in the front of
the PLC Purification System, below the screen, four
on the right side, and one more on the left side Power
Indicator Stylus
of the PLC Purification System. USB cables and all
Light
other external connections should not exceed two
meters (6.5 ft.). Figure 19
Touchscreen PC

30 Introduction | PLC 2050/2250/2500 Purification Systems

External Connections
Additional external connectors, such as an RS-232 serial communication port (COM4), LAN port,
analog signal input, and input/output ports can be used to connect external devices to the
PLC Purification System.

Local

Detailed Description
Area
Analog RS-232 Serial Network
Signal USB External Communications (LAN)
Inputs Port Controls Port (COM4) Port

Figure 20
Analog Inputs, USB Port, and I/O Ports Figure 21
(Left Side) RS-232 and LAN Ports (Right Side)

RS-232 (COM4) PORT

The RS-232 port can be used to control an external device via serial communication with GLIDER,
such as a VERITY® CPC System, the GX-241 Liquid Handler, or an additional detector.

USB PORTS
The USB ports can be used to connect any USB peripherals (keyboard, mouse, USB drive,
printer, etc.) or optional acquisition module for additional detectors, or to control the
VERITY 4020 Syringe Pump.

LAN PORTS
The LAN port can used to make direct data transfer to a private server.

ANALOG SIGNAL INPUTS

The analog input connectors (red +, black -) can be used for signal acquisition of additional
detectors (-1 V𝌂 ; +1V𝌂). A 10-bit A/D converter on an electronic board allows to convert the
analog signal to digital signal for the plotting in GLIDER.

EXTERNAL CONTROLS
The inputs 'Start In' and 'Stop In' can be used to start or stop the pump via a pulse (with 10 mA
current limitation) or a closure of a NO/NC contact (O>C) coming from an external device (max.
5 V𝌂). The outputs are static relay contacts (max. 5 V𝌂). 'Start Out' induces a short closure of a
contact NO/NC (Open>Close) at each starting of the pump (Run/Resume). 'Stop Out' induces a
short closure of a contact NO/NC at each stop of the pump (Stop/Pause).

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 31

 Terms and Abbreviations
ACRONYM DESCRIPTION ACRONYM DESCRIPTION

AC Alternating current MS Mass spectrometry

Terms and Abbreviations

CPC Centrifugal partition chromatography NC Normally closed

CCC Counter-current chromatography NO Normally open

CMOS Complementary OD Outer diameter

metal–oxide–semiconductor

DAD Diode array detector PC Personal computer

DMF Dimethylformamide PCTFE Polychlorotrifluoroethylen

DMSO Dimethyl sulfoxide PEEK Polyether ether ketone

ELSD Evaporative light scattering detector PFA Perfluoroalkoxy alkane

EMC Electromagnetic compatibility PLC Preparative liquid chromatography

ETFE Ethylene tetrafluoroethylene PPE Personal protective equipment

FCC Federal Communications Commission PTFE Polytetrafluoroethylene

FEP Fluorinated ethylene propylene RSD Repeatability standard deviation

GFP Graphite fiber-reinforced PTFE SS Stainless steel

GLP Good laboratory practice TPI Threads per inch

HPLC High performance liquid USB Universal serial bus

chromatography

ID Inner diameter UV Ultraviolet

IPA Isopropyl alcohol UV-VIS Ultraviolet–visible

LAN Local area network WEEE Waste electrical and electronic

equipment

Customer Service
Gilson, Inc. and its worldwide network of authorized representatives provide customers with
the following types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your local Gilson representative. Specific contact
information can be found at www.gilson.com. To help us serve you quickly and efficiently, please
refer to Before Calling Us on page 73.

32 Introduction | PLC 2050/2250/2500 Purification Systems

 Chapter 2

Chapter 2 | INSTALLATION

IN THIS CHAPTER:

● Place the Solvent Bottle Tray | 34

● Plumbing Connections | 34
● Column Setup | 40
● Install the Racks | 42
● Install the Stylus | 43
● Make the Power Connection | 43
● Install Aftermarket Options | 44

WARNING The installation must be performed only by qualified personnel.

A PLC Purification System and its components should be set up and installed in order per the
instructions in this chapter.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 33

 Place the Solvent Bottle Tray Tray
Place the solvent bottle tray on top of the
PLC Purification System. Gripped feet allow it to stay
in place. It is designed to minimize leakage risks and
avoid bottle falls.
Place the Solvent Bottle Tray

Plumbing Connections
Wear appropriate personal protection
CAUTION equipment (PPE) according to the solvent
pumped.
Figure 22
The pump is shipped with high-pressure stainless steel
Solvent Tray
tubing connected, and with plugs on the inlets and
outlets. Remove the plugs before connecting any
tubing.

Inlet Tubing
Inlet tubing connects the gradient valve to the solvent
reservoirs. Use the two (standard) or four (optional)
inlet lines supplied. Take care not to kink the inlet tubing.
Each inlet line has a fitting and spring on one end and
an SS ballast on the other end.
For each inlet line, place the ETFE ferrule into the PEEK
body of the gradient valve and screw on the PEEK nut,
finger tight, and slide the spring to place its end in
the knurled part of the PEEK nut. Springs are used to
prevent the tubes from bending. Colored cable ties are
provided to easily identify the tubing (A=red, B=green,
C=blue, and D=yellow).
Put the ends of the tubing with ballasts into the
appropriate solvent reservoirs.
An adjustable, releasable clip on the left side of the
system allows users to properly guide the tubing into
the bottles positioned on the solvent tray atop the Figure 23
Springs and Colored Cable Ties
system.

Ballast
PEEK
Ballast
Nut
Tubing

Figure 24 Figure 25
Inlet Tubing Ballasts Close Up of Ballast and Fitting

34 Installation | PLC 2050/2250/2500 Purification Systems

TUBING CONNECTIONS AND COMPONENTS DIAGRAM
Refer to the following diagram and table before connecting the inlet tubing.

Plumbing Connections
L

Gradient
(Solvent) F
Reservoirs
K

Left
Side

A B C D H

G N
F
E
F

A11 M
B11
C1 K
D1
Waste

Figure 26
Component and Connection Diagram for the Gradient Valve and Waste Tubing

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 35

 Gradient Valve Components and Connections
Component Description Component Description

A, B, C, and D Gradient valve ports H Waste outlet bulkhead union for

• Binary (standard) 1/8" (OD) tubing (PN 21040164)
Plumbing Connections

• Quaternary (optional)

A1, B1, C1, and D1 Solvent inlet tubing assembly K SS ballast

• Binary (qty. 2)
• Quaternary (qty. 4)
• PTFE tubing, 1/8" (OD), 2.4 mm (ID)
with a 20 cm spring, PEEK nut, and
ETFE ferrule (PN 21040101) for
PLC 2050 and PLC 2250
• PFA tubing, 3/16" (OD), 1/8" (ID)
with PEEK nut, and ETFE ferrule
(PN 21040102) for PLC 2500

E Plug L Solvent bottle tray

• PN 49041019 for PLC 2050 and
PLC 2250
• PN 21040196 for PLC 2500

F PEEK nut M Gradient valve to pump heads

• PN 21040194 (short) and tubing assembly
PN 21040195 (long) for PLC 2050 • PTFE tubing, 1/8" (OD), 2.4 mm
and PLC 2250 (ID) with springs, PEEK nuts, ETFE
• PN 49040132 for PLC 2500 ferrules, and tee union for PLC
2050 and PLC 2250
• PFA tubing, 3/16" (OD), 1/8" (ID)
with PEEK nuts, ETFE ferrules, and
Y assembly for PLC 2500

G ETFE ferrule N Waste outlet tubing assembly

• PN 21040193 for PLC 2050 and • ETFE tubing, 1/8” (OD), 1.6 mm
PLC 2250 (ID) with SS nut and ferrule
• PN 490410133N for PLC 2500) (PN 21040115) for PLC 2050 and
PLC 2250
• PTFE tubing, 1/8” (OD), 2.4 mm
(ID) with SS nut and ferrule
(PN 21040116) for PLC 2500

36 Installation | PLC 2050/2250/2500 Purification Systems

Gradient Valve to Pump Heads
The gradient valve to pump heads tubing assembly connects the gradient valve outlet to the
two pump heads via a tee union. The tubing is equipped with protective springs. Take care not
to kink the tubing.
To make the connections:

Plumbing Connections
1. Screw two PEEK nuts into the check valve housings beneath the pump heads without fully
tightening them.
2. Screw the PEEK nut into the gradient valve outlet on the left side of the PLC Purification System.
3. Finger-tighten the three fittings, and then ensure that the three fittings on the tee union are
fully tightened.

Gradient
Valve Pump
Outlet Heads

Check
Valve
Housings

Tee
Union

Figure 27
Gradient Valve to Pump Heads Connections

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 37

 Stainless Steel (SS) Fittings Assembly
1. Slide the nut and ferrule onto the tubing in the order shown on the figure.
2. Insert this assembly into the fitting body, screwing the nut in two or three turns by hand.
3. Push the tubing all the way forward into the body so that it seats firmly. This is essential for
Plumbing Connections

a proper zero dead volume connection.

4. Manually turn the nut into the body until it is finger tight.
5. Using the appropriate open end wrench, turn the nut 1/4 turn (90°) past the point where the
ferrule first starts to grab the tubing. The amount of force required can vary considerably
due to the friction between the nut and the threads and the composition and wall thickness
of the tubing used. Because of these variables, a torque specification is unreliable.

Nut
Ferrule
Tube

Fitting
Body

Figure 28
Outlet Tubing Assembly

Waste Outlet Tubing

Waste
Outlet

Figure 29
Waste Outlet

The waste tubing (PN 21040115 for PLC 2050 and PLC 2250; PN 21040116 for PLC 2500) connects
the waste outlet to a waste reservoir. There is an SS fitting at one end and an SS ballast on the
other end.
Connect the tubing to the SS bulkhead union labeled 'Waste', located on the left side of the
system. Tighten the SS nut with a 3/8” spanner, and then place the other end in suitable waste
receptacle.

38 Installation | PLC 2050/2250/2500 Purification Systems

Purge Valve Outlet Tubing
The purge valve outlet tubing (PN 21040168) connects the pump valve outlet to a waste reservoir.
There is an SS fitting at one end and an SS ballast on the other end.
Connect the tubing to the purge valve outlet, tighten the SS nut with a 3/8” spanner, and then
place the other end into suitable waste receptacle.

Plumbing Connections
Purge
Purge Tubing
Valve
Outlet

Figure 30
Purge Valve Outlet

Sample Loop

Most PLC Purification Systems come with the sample loop pre-installed at the factory; however, for
NOTE
custom orders, please follow the instructions below.

To make the sample loop connection:

● Connect a sample loop to Port 3 and Port 6 of the automatic loop injection valve.
● Connect a sample loop to Port 1 and Port 4 of the manual loop injection valve. The manual
loop injection valve is mounted on a bracket on the left side of the system.
The sample loop must be equipped with SS nuts for 1/8” tubing (5/16"-24 UNF model) and SS
NOTE ferrules for 1/8” tubing. Use a 3/8” or 5/16” spanner to tighten the nuts and properly crimp the
ferrules.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 39

 Injection Valve Waste Outlet

NOTE Used only for the automatic loop injection valve.

The injection valve waste outlet (PN 21040169) tubing connects the waste outlet of the injection
valve to a waste reservoir. There is a fitting at one end and an SS ballast on the other end. Connect
Column Setup

the tubing to the shut-off valve outlet and finger-tighten the PEEK nut. The shut-off valve is
connected to port 2 of the automatic loop injection valve.

Waste
Outlet

Shut-
Off
Valve
Figure 31
Injection Valve Waste Outlet

Column Setup
A wide range of columns can be used with PLC Purification Systems, including normal- or
reverse-phase columns and those with an overall external diameter up to 40 mm. The device used
to install the column is a clamp with vinyl coating. The clamp is mounted on the black holder on
the left side of the PLC Purification System.

Clamp
Holder
Screws

Wing
Nut

Figure 32
Column Positioning

40 Installation | PLC 2050/2250/2500 Purification Systems

To install the clamp: A

1. Slide the rod horizontally into the black holder. Tighten

the two cylindrical-head screws with the 3 mm Allen F
wrench supplied. G

2. To install the column, loosen the wing nut, and then C

Column Setup
G
slide the column inside the clamp. Securely tighten the
clamp. H

3. Connect the column inlet to the loop injection valve

E
outlet (manual or automatic).
● Port 5 for the automatic loop injection valve
● Port 3 for the manual loop injection valve B

4. Connect the column outlet to the bulkhead union

detector Inlet.
If additional valves are installed, such as the
automatic backflush valve (PN 21040005 for
PLC 2050; PN 21040006 for PLC 2250 and
NOTE PLC 2500) or manual column switching valve D
(PN 21040013 for PLC 2050; PN 21040014 for
PLC 2250 and 2500), proceed as indicated on the
system.
Figure 33
5. Use a 3/8” spanner to tighten the nuts and crimp the Column Connections to Automatic
ferrules. Loop Injection Valve

Column Connections to the Automatic Loop Injection Valve

Component Description Component Description

A Bulkhead union detector inlet E Automatic loop injection valve

B Column F Column tubing, from column

outlet to bulkhead union
detector inlet

C Column outlet/inlet G Column tubing, from automatic

loop injection valve (Port 5) to
column inlet

D Column outlet/inlet H Sample loop

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 41

 Install the Racks
PLC Purification Systems accommodate three racks. The standard, supplied rack set
(PN 21040019) consists of three SS racks with 192 tubes, 180 mm high with a total collection
volume of 6.1 L.
Dispense
Remove the black cable tie which
Install the Racks

Arm
holds the collector arm during
NOTE
transport by manually loosening it.
Keep this cable tie, it is reusable.

To install the racks:

1. Place the tubes in the rack holes, and then Cable

place the racks on the collector plate. Tie

2. Locate the middle slot in the rear of the

rack. Slide the slot over the back positioning Figure 34
guide mounted on the collector plate. Collector Arm Cable Tie
3. Fit the middle slot on the front of the rack
over the positioning guide in front of the collector plate.

When using short racks, use the additional positioning guide supplied and place it in appropriate
NOTE
holes.

Back
Guide

Back Guide
for
Short Racks

Rack

Rack
Slot

Front
Guide

Figure 35 Figure 36
Rack Installation Short Racks Guide

42 Installation | PLC 2050/2250/2500 Purification Systems

Install the Stylus Set
Screw Stylus
Use the supplied stylus to make software
selections on the touchscreen. A fixed
holder is provided for the stylus and its
extendable cord. The stylus holder is installed

Install the Stylus

with the supplied screw on the right side
of the PLC Purification System through the
threaded hole.

Make the Power Connection

The PLC Purification System is supplied with
the appropriate fuses and is ready to operate
at the line voltage of the destination country. A
voltage selector located near the power
receptacle allows users to adjust the input Figure 37
voltage of the system. Check that the position Stylus and Holder
of the voltage selector corresponds to the
voltage of the mains.

Never use any cabling not supplied or recommended by Gilson. Use of unspecified cabling may
NOTICE
lead to improper operation or failure to comply with safety or EMC regulations.

When operating the PLC Purification System, it must be possible to disconnect it from mains
supply at any time. In the event of an emergency, the power connector of the instrument must
be easily accessible and removable.
CAUTION The PLC Purification System must never be operated from a power outlet that has no ground
connection. The absence of a ground connection can lead to electric shock or short circuit.
The PLC Purification System is designed for use with liquids; however, liquid contact with
external equipment may lead to the risk of electric shock or short circuit. Ensure that fluid
connections are not close to ancillary equipment and are checked for leaks prior to use. In the
event of a leak, any ancillary equipment not designed for use with liquids must be turned off
until the liquid is removed.

Power
To make the power connection, plug Fuse Receptacle
in the AC power cord into the power Drawer
receptacle located on the right side Voltage
Power Selector
of the PLC Purification System. Then
Switch
make the connection between the
PLC Purification System and the
AC power source.
● 110–120/220–240 V~ (single-phase)
● 50/60 Hz
● 450 W max.
Figure 38
● General fuses: T 6.3A H 250 V~ (qty. 2) Power Switch and Voltage Selector

| indicates that the electrical power is switched on; O indicates that the electrical power is
NOTE
switched off.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 43

 Install Aftermarket Options
Install the Manual Column Switching Valve
The manual column switching valve (PN 21040013 for PLC 2050; PN 21040014 for
PLC 2250 and PLC 2500) connects two columns to a single flow path; e.g., a column installed
Install Aftermarket Options

on the standard column clamp and an additional column. Secure the additional column with a
second column clamp or use a standalone column holder (PN 21040015).

Manual Automatic
Column Column
Switching Backflush
Valve Valve

Figure 39
Column Switching Valve with Flow Diagrams

To install and plumb the manual column switching valve:

1. Secure the manual column switching valve (already mounted on its bracket) to the two
dedicated holes located on the left side of the PLC Purification System with the two
domed-head screws supplied with a 3 mm Allen wrench.
2. Connect the manual column switching valve inlet (Port 1) to the loop injection valve outlet.
3. Connect the column switching valve outlet (Port 4) to the bulkhead union detector inlet.
4. Connect the columns inlets to the column switching valve:
● To Port 2 for the Column 1 inlet
● To Port 6 for the Column 2 inlet
5. Connect the columns outlets to the column switching valve:
● To Port 3 for the Column 1 outlet
● To Port 5 for the Column 2 outlet
6. Use a 3/8” spanner to tighten the nuts and properly crimp the ferrules.
7. Switch the manual switching valve to the right to direct flow to the Column 1. Switch the
valve to the left to direct flow to Column 2.

Do not switch the manual column switching valve while the pump is running as internal
NOTICE
damage may occur due to a sudden overpressure.

44 Installation | PLC 2050/2250/2500 Purification Systems

Install the Standalone Column Holder for LC Columns
When flash chromatography
columns are used with the
system, use the standalone
column holder (PN 21040015) for

Install Aftermarket Options

LC Columns. It is equipped with
low-pressure Luer connections
and fittings for easy injection on
top of the column. The maximum
overall external diameter is
115 mm and the maximum overall
height is 400 mm.

Figure 40
Standalone Column Holder

Install the Acquisition Module for Additional Detectors

The system is equipped with a built-in analog input to acquire the signal from an additional
detector. To improve the quality of the external detection and increase the number of signals
acquired, the acquisition module for additional detectors can be used. It allows users to plot
more precise curves and acquire up to four signals simultaneously with GLIDER.
Follow the procedure supplied with the module to install the requested drivers, connect it to the
system (via a USB port), and then to the analog output(s) of external detector(s).

Figure 41 Figure 42
Acquisition Module Acquisition Module Connections

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 45

 Install the Fraction Collector Cover Kit
This option can be mounted on PLC Purification Systems placed outside of a fume hood to allow
the extraction of fraction collector vapors. The transparent door is secured with a magnet. An
adaptor, located on the right side of the PLC Purification System, is used to affix a pipe (not
supplied) with a metallic clamp. The pipe should then be connected to an external air extractor.
Install Aftermarket Options

Collector
Cover
with
Magnetic Metallic
Lock Clamp
Transparent
Door

Adapter for
Pipe Connection
and
Air Extraction

Figure 43
Fraction Collector Cover

Install the Rack Overlays

Rack overlays for 18/16 mm tubes and 13/12 mm tubes
are designed for glass tubes identification. They
correspond to the default collection probe trajectory
called 'Alternating Vertical' in GLIDER.
The characters inscribed on the front correspond to
number order, whereas those on the back are related to Figure 44
the physical position of tubes in the rack (n° of rack + n° Collector Racks with Overlays
of tubes in the rack).

Figure 45
Overlays for Racks 18 mm Tubes (Front Side Numbering)

Figure 46
Overlays for Racks 18 mm Tubes (Back Side Numbering)

46 Installation | PLC 2050/2250/2500 Purification Systems

Install the Funnel Rack
The 16-outlet funnel rack accommodates large and variable collection volume in external
high-capacity containers. The funnel rack is installed directly on the collector tray at the front of
the PLC Purification System and replaces the standard racks.

Install Aftermarket Options

Figure 47
Funnel Rack

To install the funnel rack:

1. Use a 2.5 mm Allen wrench to remove the two cylindrical-head screws from the front
positioning guide.
2. Remove the front positioning guide from the tray.
3. Place the funnel rack on the tray and fix it with the two cylindrical-head screws previously
removed. Tighten the two screws with the 2.5 mm Allen wrench.
4. Connect the 16-outlet tubing and securely tighten the black nuts.

Front Front
Guide Guide
Screws Screws

Figure 48
Funnel Rack Installation

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 47

 Chapter 3

Chapter 3 | OPERATION

IN THIS CHAPTER:

● Start Up | 50
● Prime the Pump | 51
● Loop Injection with Automatic Valve | 52
● Loop Injection with Manual Valve | 53
● Power Down | 54

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 49

 Start Up
To start the PLC Purification System:

1. Turn the power on using the switch on the side panel (Refer to External Connections on page 31).
● The power indicator light below the touchscreen on the front panel illuminates.
Start Up

● The fan starts.

● The automatic valve(s) status LEDs on the front panel illuminates according to the
position of the valve(s).
● The system initializes as the fraction collector arm goes to home position.
● The PC starts as the green ‘Power Indicator’ light on the screen frame illuminates, and
the red ‘Hard Drive Activity’ light flashes to indicate that the storage device is being
used.
● The screen switches on and displays the
Microsoft® Windows® logo. After one
minute, the Windows desktop appears.
2. Wait for GLIDER Software to load.
● The software user interface opens and
communication is established with
devices.
● The automatic valve(s) switch to suitable
positions.
● The UV-VIS detector lamp turns on, and
the two rear fans of the detector start.
● Once the software is loaded, several
graphics will appear, displaying in
real time the status of the instrument Figure 49
GLIDER Software Control Screen
and related components. Refer to the
GLIDER Software User’s Guide for more
information.

NOTICE Do not use sharp tools to touch the screen. Use the stylus or your fingers.

50 Operation | PLC 2050/2250/2500 Purification Systems

Prime the Pump
Purge
When tubing is empty and the pump is unprimed, Valve
it may be difficult to pump new solvent into the Outlet
system. To ease this process:
Knurled
1. Launch the Purge Mode in GLIDER. (Refer to Knob

Prime the Pump

the GLIDER Software User’s Guide.)
2. Loosen the knurled knob (one-half turn
counter‑clockwise) of the purge valve located
above the pump heads.
3. Once solvent has flowed through the purge
outlet tubing, stop the pump and tighten the
knurled knob (clockwise) to close the purge
valve.

Figure 50
Purge Valve Knurled Knob

Figure 51
Close Purge Valve Message

For best results, elevate the solvent containers for priming the pump. Or, connect a large
NOTICE
volume syringe to create a slight vacuum at the outlet.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 51

 Loop Injection with Automatic Valve
Use the six-way automatic loop injection valve to manually load the sample in the sample loop
and inject it automatically in the column. Refer to PLC with Configuration on page 89 when using
the GX-241 Liquid Handler as an autosampler.
The sample loop should be loaded when the PLC Purification System is stopped or during an
Loop Injection with Automatic Valve

equilibration phase. In both cases, the red LED Load light will illuminate.

Figure 52
Automatic Load and Injection Diagrams

To inject the sample in the column:

1. Fill a syringe with the desired volume of sample.

2. Place the syringe in the Luer female fitting in the top position of the injection valve (Port 1).
3. Load the sample into the sample loop. Any surplus will flow through the waste tubing
(Port 2).
● When the injection mode Valve Injection is selected in the method edition (refer to the
GLIDER Software User’s Guide), the sample is automatically injected before the elution
phase starts, as indicated by the green LED Inj. light. The sample is pushed into the
fluid path and is pushed into the chromatography column by the solvents used.

Figure 53
Injection Mode Setting

52 Operation | PLC 2050/2250/2500 Purification Systems

Loop Injection with Manual Valve
Use the six-way manual loop injection valve to manually load the sample into the sample loop and
manually inject it into the column. This action can be done at any time while the pump is running.

Mobile phase spray from the needle port may occur when the valve is turned from the

Loop Injection with Manual Valve

CAUTION INJECT to LOAD position. Use the needle port plug, attached to the handle assembly, to
shield yourself from this potential spray.

Rinse the valve with water after using buffer solutions to prevent crystals from forming,
NOTICE
which can cause scratches on the rotor seal of the valve.

The sample loop should be loaded when the system is stopped or during an equilibration phase.
To inject the sample into the column:

1. Rotate the manual injection valve handle to the LOAD position.

2. Fit the large-bore syringe needle (supplied) on a syringe and fill it with the desired volume
of sample.
3. Pull the locking pin to unlock and remove the needle port plug.

Locking
Pin

Needle
Port
Plug
Needle
Port

Figure 54
Manual Injection Valve - Needle Port

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 53

 4. Insert the syringe with the needle into the needle port and load the sample into the sample
loop. Any surplus will flow through the waste tubing (Port 6).
Loop Injection with Manual Valve

Figure 55
Manual Injection - Load and Inject Diagrams

5. Switch the valve from the LOAD to INJECT position with a 60° rotation of the handle.
● The sample is pumped into the fluid path and pushed into the chromatography column
via the mobile phase used.
● When the injection mode Wait for Injection is selected in the method edition (refer to
the GLIDER Software User’s Guide), the elution phase will start upon user confirmation
that the valve has been manually switched.

Figure 56
Injection Mode Setting - Wait for Injection Message

Power Down
1. Stop the pump and the running method.
2. Close GLIDER.
3. Turn off the PC and wait for the touchscreen to turn off.
● The UV-VIS detector lamp will remain on.
4. Switch the main power switch to ‘0’. Refer to External Connections on page 31.
● All indicator lights turn off.
● The UV-VIS detector lamp switches off.
● The fans stop.

54 Operation | PLC 2050/2250/2500 Purification Systems

 Chapter 4

Chapter 4 | MAINTENANCE

IN THIS CHAPTER:

● Helpful Hints | 56
● Cleaning and Decontamination | 56
● Maintenance Schedule | 58
● Replace a Pump Seal | 59
● Clean or Replace a Pump Check Valve | 62
● Clean the Pump Pistons | 64
● Replace the Mixing Chamber Piston Seal | 65
● Test and Repair the UV-VIS Detector | 66

This chapter describes actions that should be performed on a routine basis to ensure the long
term safe and trouble-free operation of the PLC Purification System. The frequency of the
maintenance activities is dependent on the nature of the application, such as the solvents used,
the volume of the mobile phases delivered by the pump, the level of cleanliness of the facility,
etc. The PLC Purification System has been designed for reliability and needs very little routine
maintenance when operated correctly.
When performing the maintenance described in this chapter, use good laboratory practice (GLP),
including, but not limited to, wearing protective clothing and preparing the maintenance space for
service. Only qualified personnel can perform the maintenance. After completing the maintenance
operation, verify the safe and good working order of the part and PLC Purification System.
Three models of pump are available in the range (50 mL/min, PLC 2050; 250 mL/min, PLC 2250;
and 500 mL/min, PLC 2500). Parts of the heads kit are different according to the pump model
(seals, pistons, cleaning discs, heads, etc.).
Reduced accuracy of the pump may be caused by seal wear or by a fault in the check valves. This
chapter contains information and procedures for replacing consumable parts and maintaining
the PLC Purification System.

Do not open the PLC Purification System or attempt to perform any maintenance other than
the ones described in this chapter, failure to comply with this directive will void the warranty
WARNING and may be potentially dangerous.
Before performing any maintenance, make sure that the PLC Purification System is unplugged
and that the fluid paths are flushed and decontaminated according to the cleaning procedure.

NOTICE Follow local regulations when discarding any used fluidic parts.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 55

 Helpful Hints
To keep the system at optimal performance, Gilson recommends the following:
● Follow the preventative Maintenance Schedule on page 58.
● Do not start the pump without fluid in the system as this may prematurely wear the seals.
Helpful Hints

● Do not leave buffer in the system. Doing this may cause blockages and wear the seals.
● Run a clean and appropriate solvent through all fluid paths before storing the system.
● Clean the valves if the system has not been used for a while.
● Wipe up all spills immediately.
● Allow liquids to equilibrate to room temperature before running them through the system;
cold liquids may cause leakage.

Cleaning and Decontamination

Liquids emitted by the pump (spills or grease) must be collected according to GLP. Holes at the
bottom of the pump housing allow the recovery of liquid.

Exterior
The instrument should be cleaned occasionally, using a dry, clean cloth. If necessary, use a cloth
dipped in water. If liquid is accidentally spilled on the instrument, wipe it up immediately. If a
contamination is suspected, the external surfaces of the instrument should be wiped down with
a laboratory detergent (alkaline) and rinsed with water and then with a 10% ethanolic (or IPA)
aqueous solution (v/v) to remove any residue.

WARNING Beware of splashes when cleaning the fluidic panel. Wear appropriate PPE.

CAUTION Do not to use a detergent that will damage the paint or pollute the work environment.

56 Maintenance | PLC 2050/2250/2500 Purification Systems

Fluid Path
Depending upon your use of the PLC Purification System, it may be necessary to flush the entire
fluid path. It is important to clean the fluid path if the pump will not be used a while, for weekend
or longer storage, or if a solution with a high salt concentration was used for a wash or as a diluent.
Refer to the instructions below:

Cleaning and Decontamination

1. Prime the fluid path with distilled or deionized water, or with a solution adapted to the use
of the instrument.
2. Flush the fluid path with an alcohol/water mixture, ratio between 30/70 up to 100/0 (v/v).

Before running an application, prime and flush the fluid path with the solvents of this
application.
After having run an application, flush the fluid path with the suitable mixture and let those
NOTICE
clean solvents in the fluid path. Alcohol prevents bacterial development.
Only use water and ethanol to clean the system so not to damage the seals, cables, tubing
and all the plastic parts.

WARNING
Potentially hazardous chemicals can be used with the system. Use care when handling
chemicals and wear appropriate PPE. Handling toxic, flammable, and hazardous chemicals
can lead to health and safety risks.

The pump pistons can also get dirty and interfere with the fluid path, refer to
Clean the Pump Pistons on page 64.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 57

 Maintenance Schedule
Gilson recommends performing periodic inspection and maintenance of components of
PLC 2050/2250/2500 Purification Systems to ensure operational performance. The recommended
inspection and maintenance periods are listed below, but are only general guidelines. The
frequency of the maintenance will vary depending on the system usage and type of sample
Maintenance Schedule

injected.

NOTICE Follow all guidelines listed in this table to avoid damaging the PLC Purification System.

Maintenance Schedule
OPERATION FREQUENCY

Verify the cleanliness of all liquid containers. Daily

• Clean and rinse the gradient valve and all other switching valves with suitable solution. As needed
• Perform maintenance any time an aqueous solution with strong buffers is used.

• Clean all parts in contact with solvents or samples with suitable solutions. Rinse thoroughly using a As needed
mix of water and alcohol.
• Perform maintenance any time an aqueous solution with strong buffers is used.

Replace the detector deuterium lamp when efficiency is unreliable. Refer to the As needed
UV-VIS Detector Service Guide.

• Check the suitable tightening of fittings and the proper state of tubing (not kinked, damaged, etc.). Weekly
• Perform maintenance any time solvent leaks or air bubbles are observed.

Check that the fan is operational. Monthly

Clean the pump check valves. Refer to Clean or Replace a Pump Check Valve on page 62. Quarterly

Clean the pump piston rods (manually). Refer to Clean the Pump Pistons on page 64. Perform Quarterly
maintenance any time the pump head is disassembled.

• Check the efficiency of the detector deuterium lamp. Refer to the UV-VIS Detector Service Guide. Quarterly
• Perform maintenance any time higher noise levels or decreased sensitivity is observed in the
chromatogram.

Clean the detector flow cell. Refer to the UV-VIS Detector Service Guide. Quarterly

• Replace the tubing and associated fittings (except the preformed, SS tubing). Yearly
• Replace damaged tubing and fittings any time damage is observed.

Replace the pump seals. Refer to Replace a Pump Seal on page 59 Yearly

• Replace the pump check valves. Refer to Clean or Replace a Pump Check Valve on page 62. Yearly
• Perform maintenance any time abnormal pressure, flow rate fluctuation, or noisy baselines occur.

58 Maintenance | PLC 2050/2250/2500 Purification Systems

Replace a Pump Seal
The lifetime of the seals is dependent on the flow rate, the pressure, and the type and
temperature of liquids used, but mostly on the cleanliness of the mobile phase and sample.
The presence of micro-particles will cause accelerated wear and tear of the seals. Similarly,
NOTICE

Replace a Pump Seal

any dried buffer particles on the piston will damage the seal.
After performing a maintenance on a fluidic part, check for leakage by running an application
and gradually increase the pressure to the maximum.

When disassembling or reassembling the pump, make sure that each component is clean and
take care that the system is assembled in a clean environment.
There are four seals: one for each pump head, and one for each cleaning disc.
To disassemble a pump head with cleaning disc, and to replace a seal, follow the instructions
below:

Remove the Pump Head and the Cleaning Disc

1. Purge the system with a suitable solvent.
2. Switch off the PLC Purification System and disconnect it from the mains. (Refer to
External Connections on page 31).
3. Disconnect the PTFE or PFA inlet tubing by hand (PEEK nut).
4. Disconnect the SS outlet tubing with a 3/8" spanner (SS nut). When untightening the SS
nut, use a second spanner (17 mm) to hold the check valve housing. The tubing must be
removed (i.e., disconnect it from the purge valve holder at the other end).
5. Loosen the four M4 screws (counter‑clockwise) of the head in an alternating criss-cross
pattern with a 3 mm Allen wrench.
6. Hold the pump head and consecutively pull out the four screws.

SS
Outlet
Tubing

Check
Valve
Housings

ETFE Inlet Tubing with PEEK Nuts

(Below Check Valve Housings)

Figure 57 Figure 58
Removing the Outlet and Inlet Tubing Removing the Head Screws

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 59

 7. Carefully remove the pump head. The ceramic piston and the cleaning disc (PEEK part) will
be visible.
Replace a Pump Seal

Cleaning Exposed
Disc Piston

Cleaning Exposed
Discs Pistons

Figure 59 Figure 60
Removing the Pump Heads Removing the Cleaning Discs

8. Carefully remove the cleaning disc to fully expose the piston.

If needed, disconnect the ETFE junction tubing for cleaning discs by hand (PEEK nuts under
NOTE
pump head).

9. Once the part is removed, carefully take out the old seal.

Do not score the seat of the seal. If the seat is scored, it is necessary to replace the pump
NOTICE
head or the cleaning disc (not under warranty).

Seal Seal Seat

Figure 61
Back Pump Head and Back Cleaning Disc

60 Maintenance | PLC 2050/2250/2500 Purification Systems

Replace the Seal
1. Thoroughly clean the seal seat with IPA or ethanol to remove possible worn seal particles,
and then plunge the new seal in IPA for lubrication.
2. Align the new seal over the seal seat of the head or cleaning disc.

Replace a Pump Seal

B

B
A

A
Seal

A B

Figure 62
Pump Seal Seating Instructions

3. Use the pump seal insertion tool, A and B, pictured above.

4. Position the part A over the back of the pump head or of the cleaning disc and slip the part
B into A.
5. Push briskly the part B with the palm of your hand, inserting the seal into position.
6. Remove the tool and examine the seal to ensure it is properly placed.
7. Clean the piston rod with a lint-free cloth and alcohol to remove possible worn seal particles.
8. Replace carefully the cleaning disc onto the piston and insert it into the head holder. Verify
that the alignment between the cleaning disc and the head holder hole is correct.
9. Replace carefully the pump head onto the piston by checking that the outlet check valve
housing is on top, and the inlet check valve housing is at the bottom.
10. Insert the four fastening screws. Tighten slowly in an alternating criss-cross pattern with a
3 mm Allen wrench.
11. Reconnect the tubing. When tightening the SS nut on the outlet check valve housing, use a
17 mm spanner to hold the housing and keep the suitable tightening.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 61

 Clean or Replace a Pump Check Valve
After performing a maintenance on a fluidic part, check for leakage by
NOTICE
running an application and gradually increase the pressure to the maximum.

Abnormal pressure fluctuation or the observation of pump noise in the

Clean or Replace a Pump Check Valve

chromatogram data is usually caused by check valve problems. Dirty or

damaged check valves do not open and close properly, which can cause
pressure fluctuations and irregular flow.
There are four check valves: two per head, one for each inlet, and one for each
Figure 63
outlet. Check Valve
To clean or replace a check valve, follow the instructions
below: Purge Valve Inlets
1. Purge the system with a suitable solvent.
2. Switch off the system and disconnect it from the
mains. (Refer to External Connections on page 31). Outlet Outlet
Check Check
3. For an inlet check valve (bottom), disconnect the Valve Valve
PTFE or PFA inlet tubing by hand (PEEK nut).
4. For an outlet check valve (top), disconnect the SS
outlet tubing with a 3/8" spanner (SS nut). When
untightening the SS nut, use a second spanner
(17 mm) to hold the housing. The tubing must be
removed (i.e., disconnect it from the purge valve
holder at the other end).
5. Once the tubing is removed, unscrew the check
valve housing using a 17 mm spanner and remove Inlet Check Valve, ETFE Tubing, and PEEK Nut
the check valve. If the check valve is blocked inside
the housing, use a small tool to extract it, in this case, Figure 64
the check valve must be replaced. Removing the Outlet and Inlet Tubing

Figure 65
Removing the Check Valve Housing and the Check Valve

62 Maintenance | PLC 2050/2250/2500 Purification Systems

6. Clean the check valve or replace the check valve with a new one:
a. Clean the check valve by placing it in an ultrasonic bath with methanol or acetone for
approximately five minutes.

Ensure that the arrow on the check valve is directed downwards when it is cleaned in
NOTICE
an ultrasonic bath, or damage to the surface quality, the ball and the seat will occur.

Clean or Replace a Pump Check Valve

b. Replace the check valve. Whether it is for the inlet or the outlet, make sure the arrow is
always directed upwards.

Figure 66
Inlet Valve Housing (Bottom)
Inlet Check Valve Placement

Figure 67
Outlet Valve Housing (Top)
Outlet Check Valve Placement

7. Tighten slowly the valve housing in the pump head by hand until finger tight and tighten
the housing with a 17 mm spanner until hearing a little crunch, which means that the new
check valve is properly seated.

Do not overtighten the valve housing as this could degrade the check valve more quickly.
NOTICE
If a liquid leakage or air bubble is detected, tighten slightly to improve the sealing.

8. Reconnect the tubing. When tightening the SS nut on the outlet check valve housing, use a
17 mm spanner to hold the housing and keep the suitable tightening.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 63

 Clean the Pump Pistons
After performing a maintenance on a fluidic part, check for leakage by running an application
NOTICE
and gradually increase the pressure to the maximum.

1. Follow steps 1 through 8 in Remove the Pump Head and the Cleaning Disc on page 59.
Clean the Pump Pistons

2. Thoroughly clean the piston rods with a lint‑free cloth and alcohol.
3. Follow the steps 8 through 12 in Replace the Seal on page 61 to reinstall the cleaning discs
and pump heads.

Connect the Inlet and Outlet Tubing for the Cleaning Discs
The high pressure heads incorporate a piston washing device called cleaning discs. These are
typically used when aqueous buffers are pumped and function to manually wash the buffers
from the back of the seals and to prevent early wear of these seals.

1. Fit the 1/8" PEEK nuts and ETFE ferrules (supplied) onto suitable lengths of wide bore 1/8”
PTFE tubing.
2. Screw the fittings into the cleaning discs inlet and outlet at the back of the high pressure
heads. Both connections are interchangeable, choose one for the inlet, and the other for
the outlet.
3. Connect the cleaning discs inlet tubing to a dedicated device (a combination syringe with
Luer or another external pumping system).
4. Put the cleaning discs outlet tubing into a waste container.

Inlet
and
Outlet
for
Cleaning
Discs

Figure 68
Pistons Cleaning

64 Maintenance | PLC 2050/2250/2500 Purification Systems

Replace the Mixing Chamber Piston Seal
After performing a maintenance on a fluidic part, check for leakage by running an application
NOTICE
and gradually increase the pressure to the maximum.

To replace the seal of the mixing chamber, follow the instructions below:

Replace the Mixing Chamber Piston Seal

1. Purge the system with a suitable solvent.
2. Switch off the system and disconnect it from the mains.
3. Disconnect the SS tubing from the chamber outlet with a 3/8" open-ended spanner
(SS nut). The tubing must be removed (i.e., disconnect it from the injection valve inlet at the
other end).

Chamber M5
Outlet Screws

Figure 69
Mixing Chamber Piston Removal

4. Remove the four M5 screws with a 4 mm Allen wrench.

5. Carefully pull the piston to remove it.
6. Remove the worn seal and get a new seal.
7. Insert the seal in the seal seat of the piston.
8. Replace the piston in the mixing chamber with the threaded hole on top.
9. Replace the four fastening screws and securely tighten them.
10. Replace the tubing and securely tighten the SS nuts.

PTFE Seal for Mixing Chamber Piston

Figure 70
Mixing Chamber Piston

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 65

 Test and Repair the UV-VIS Detector
Several models of UV detectors are available with the PLC 2050/2250/2500 Purification Systems:
UV monochromator single or dual wavelength, UV or UV-VIS PDA detectors (DAD) 4-wavelength
with different wavelength ranges (200–400 nm, 200–600 nm or 200–800 nm).
A deuterium lamp is used as a source of light. In versions up to 800 nm, a secondary halogen
Test and Repair the UV-VIS Detector

lamp is used. The efficiency of the lamp and the cleanliness of the flow cell must be checked
periodically.
If efficiency of detection is unreliable, detector lamp wear could be to blame. Deuterium lamp
life can be checked with GLIDER Software. From the GLIDER main menu, select the ? icon, and
then Information.
When disassembling or reassembling the detector, make sure that each component is clean and
take care that the system is in a clean environment.
Refer to the UV-VIS Detector Service Guide for more information on detector testing, lamp
replacement, and flow cell cleaning.

66 Maintenance | PLC 2050/2250/2500 Purification Systems

 Chapter 5

Chapter 5 | TROUBLESHOOTING

IN THIS CHAPTER:

● Troubleshooting Table | 68
● Repair and Return Policies | 73

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 67

 Troubleshooting Table
The following table details some basic symptoms, possible causes, and potential solutions for issues
related to PLC 2050/2250/2500 Purification Systems. If the problem persists after all remedies
have been attempted, contact Gilson customer service. Refer to Customer Service on page 32.

Fluidics and Pumping

Troubleshooting Table

Symptom(s) Possible Cause(s) Remedy Reference

Leakage or air bubbles. The fittings are not Tighten the fittings.
sufficiently tightened.

The pump makes • The motor or motor • Stop the pump. Refer to
abnormal noises. driver is damaged or • Switch off the Power Down on page 54.
not properly installed. PLC Purification System.
• The system belt or
pulleys are damaged.

• Inaccurate or irregular • The fittings are not • Tighten the fittings and Refer to page 56.
flow rates. sufficiently tightened. check the state of the
• Lack of solvent suction. • Improper state of tubing.

• Inaccurate gradient tubing or fitting. • Replace the fittings

forming. • The fluid path is and/or the tubing, if
partially or totally damaged.
blocked. • Clean the fluid path.

• The piston rods are • Check and/or clean all Refer to

dirty. parts in contact with Maintenance on page 55.
• The pump piston seals solvents or samples
are worn. (gradient valve, piston
rods, seals, check
• The pump check valves valves, etc.).
are dirty or damaged.
• Replace the
consumable parts
(seals, check valves,
etc.), if damaged.

• Abnormal pressure The pump check valves Check and/or clean Refer to page 62.
fluctuation. are dirty or damaged. and/or replace the pump
• Noise on the data check valves.
system.

Abnormal overpressure. • Improper state of • Determine the Refer to page 56.

tubing or fitting. overpressure location
• The fluid path is by isolating different
partially or totally parts of the fluid path.
blocked. • Check the state of
tubing.
• Replace the tubing, if
damaged.
• Clean the fluid path.

FLUIDICS AND PUMPING TROUBLESHOOTING CONTINUED ON PAGE 69

68 Troubleshooting | PLC 2050/2250/2500 Purification Systems

 Fluidics and Pumping
Symptom(S) Possible Cause(s) Remedy Reference

Leakage near the pump Worn pump piston seals. Replace the pump piston Refer to page 59.
heads or cleaning discs. seals.

Troubleshooting Table
Leakage near the purge Worn purge valve seal. • Check the purge valve
valve. seal.
• Replace the purge
valve seal, if damaged.

• No pressure • Damaged pressure • Check the pressure

acquisition. transducer. transducer and its seal.
• Leakage near the • Worn pressure • Replace the pressure
pressure transducer. transducer seal. transducer and its seal
if damaged.

Leakage near the mixing Worn mixing chamber • Check the mixing Refer to page 65.
chamber. piston seal. chamber piston seal.
• Replace the mixing
chamber piston seal if
damaged.

Leakage near a • The fittings are not • Tighten the fittings and
high-pressure valve. sufficiently tightened. check the state of the
• Improper state of tubing.
tubing or fitting. • Replace the fittings
• The fluid path is and/or tubing, if
partially or totally damaged.
blocked. • Clean the valve and the
fluid path.

Leakage near the • The fittings are not Check the tubing and
UV detector flow cell sufficiently tightened. fittings connection to
(liquid flow under the • Improper state of and from the UV cell by
system through the tubing or fitting. removing the plastic
detector drain pan panel on the left side of
tubing). the system.

Cracked quartz window Replace the UV flow cell. Refer to the UV-VIS Detector
inside the UV flow cell. Service Guide.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 69

 Detection
Symptom(s) Possible Cause(s) Remedy Reference

UV detector does not • No communication. • Restart the • Refer to the UV-VIS

respond to commands. • Electronic failure. PLC Purification System. Detector Service Guide.
Troubleshooting Table

If the problem persists, • Refer to the

export the Traces log GLIDER Software
files from GLIDER. User’s Guide.
• Run a basic diagnostic
of the detector.
• Create a ‘Service
Report’ for the detector.

Noise on UV signals. • Use of non-degassed • Degas solvents Refer to the

solvents. pumped. GLIDER Software
• Unsuitable mixing of • Increase the User’s Guide.
solvents pumped. backpressure value
after the flow cell.
• Use GLIDER to make a
calculated filtering of
the UV signals.

• Higher noise levels or • Low UV lamp intensity. • Check the UV lamp for Refer to the UV-VIS
decreased sensitivity on • Unreliable efficiency of efficiency. Detector Service Guide.
UV signals. UV lamp. • Check for UV noise and
• Improper autozero. drift.
• Check the light intensity
curve.

• No signal during UV Worn UV deuterium lamp. Replace the UV deuterium Refer to the UV-VIS
acquisition. lamp and perform an Detector Service Guide.
• UV detector lamp does intensity recalibration.
not switch on.

Low signal during UV Worn UV halogen lamp. Replace the UV halogen Refer to the UV-VIS
acquisition in visible lamp and perform a scan Detector Service Guide.
range. intensity.

Lowered UV light Dirty or contaminated UV Check the UV cell for Refer to the UV-VIS
transmission, persistent flow cell. cleanliness. Detector Service Guide.
higher noise levels or
decreased sensitivity on
UV signals with efficient
lamp.

Peaks detected by UV UV flow cell optical path Replace the UV cell with Refer to the UV-VIS
have a too low intensity length is too small. another cell with a higher Detector Service Guide.
whatever the application. path length.

Peaks detected by UV UV flow cell optical path Replace the UV cell with Refer to the UV-VIS
have a too high intensity length is too large. another cell with a lower Detector Service Guide.
(signal saturation) path length.
whatever the application.

70 Troubleshooting | PLC 2050/2250/2500 Purification Systems

 Collection
Symptom(s) Possible Cause(s) Remedy Reference

Fraction collector • No communication. Restart the system. If the Refer to the

does not respond to • Electronic board problem persists, export GLIDER Software

Troubleshooting Table
commands. firmware failure. the Traces log files from User’s Guide.
GLIDER.

Fraction collector does Motor(s) or optical • Check the X and Y

not go to home position. sensor(s) are damaged or movement when system
not properly supplied. is off.
• Check the state and
cleanliness of collector
sensors.

Fraction collector does • Wrong racks used. • Check rack code in the Refer to the
not collect properly into • Wrong racks software matches the GLIDER Software
tubes. positioning. racks you are using. User’s Guide.
• Wrong collection • Check positioning of
parameters. racks on the tray.
• Check the collection
parameters in GLIDER
and then adjust if
needed.
• Check that the
collection probe is
sufficiently tightened.

Mechanical blockage. Check for obstruction to

X and Y movement when
system is off.

Dirty or damaged • Check the collection

collection valve. valve cleanliness.
• Check the collection
and waste tubing state.
• Disassemble the
collection valve for
cleaning.
• Replace the collection
valve or tubing if
damaged.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 71

 Communications, Electronics, and Power
Symptom(s) Possible Cause(s) Remedy Reference

The PLC Purification The PLC Purification • Check the AC power source.
System does not System is not supplied with • Try a different AC outlet.
Troubleshooting Table

switch on. voltage. • Check the AC power cord.

• Check the fuses and replace, if
necessary.

A communication • Incorrect configuration. • Restart GLIDER. Refer to the

error with GLIDER. • Incorrect initialization of • Restart the PC. GLIDER Software
an internal device. User’s Guide.
• Restart the system.
• No communication with • Check the
an internal device. System Configuration in
GLIDER.
• If the problem persists, export
the Traces log files from
GLIDER.

GLIDER does not An abnormal malfunction • Export the Traces log files Refer to the
work correctly. not detected beforehand. from GLIDER. GLIDER Software
• Upgrade GLIDER to the newest User’s Guide.
version.

The touchscreen PC • An unexpected shutting Manually reboot the PC by

does not turn on at down of the PC or the removing the small trapdoor on
the same time the system. the right side of the system and
PLC Purification System • General outage. by pressing the built-in push
turns on. button.

The stylus points to An unwanted modification Recalibrate the touchscreen with Refer to the
the wrong place on of touchscreen calibration. the calibration tool installed on GLIDER Software
the touchscreen. the PC. User’s Guide.

The pump does • No communication. • Restart the system. Refer to the

not respond to • Electronic board • If the problem persists, export GLIDER Software
commands. firmware failure. the Traces log files from User’s Guide.
GLIDER.

The automatic, Defective valve or control • Use GLIDER to manually switch Refer to the
high-pressure module. the valve several times. GLIDER Software
valve (injection, • Check the valve’s status LEDs User’s Guide.
backflush, column on the front panel.
switching, etc.) • Check the control module and
does not respond to its LEDs.
commands and does
not switch properly.

72 Troubleshooting | PLC 2050/2250/2500 Purification Systems

Repair and Return Policies
Refer to the following information and then contact your local Gilson representative. Specific
contact information can be found at www.gilson.com.

Before Calling Us

Repair and Return Policies

Your local Gilson representative will be able to serve you more efficiently if you have the following
information:
● Serial number and model number of the instruments involved. The serial number is located
on the right side of the PLC 2050/2250/2500 Purification System.
● List of concise symptoms.
● List of operating procedures and conditions you were using when the problem arose.
● List of other devices connected to the instrument and a description of those connections.
● List of other electrical connections in the room.

Warranty Repair
Units covered under warranty will be repaired and returned to you at no charge. If you have any
questions about applicability, contact your local Gilson representative.

Non-Warranty Repair
For out-of-warranty repairs, contact your local Gilson representative who will discuss service
options with you and can assist in making arrangements to return the equipment, if necessary.

Return Procedure
Contact your local Gilson representative to obtain authorization before returning any Gilson
equipment. To return a piece of equipment:
● Carefully pack the unit to prevent damage in transit. Check with your local Gilson representative
regarding proper method of shipment. No responsibility is assumed by Gilson or your local
Gilson representative for damage caused by improperly packaged instruments. Indicate the
authorization reference on the carton and on the packing slip.
● Always insure for the replacement value of the unit.
● Include a description of symptoms, your name, address, phone number, and purchase order
to cover repair costs, return and shipping charges, if your institution requires it.

Unit End-of-Life
When a unit reaches the end of its useful life, refer to www.gilson.com for
directives and information on the end-of life policy. This is in accordance with
the European Union Directive 2012/19/UE on Waste Electrical and Electronic
Equipment (WEEE).

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 73

 Appendix A

Appendix A | WARRANTY

IN THIS CHAPTER:

● General | 75
● Limited Warranty | 76

General
A PLC Purification System is under warranty to be free from defects in material or workmanship
under normal installation, use and maintenance, as described in this user’s guide and under
conditions given below.
The warranty period is defined in the order/contract and starts from the date of initial shipment
of the manufacturer. Note that it does not apply to the instrument if modified by the user or
resold without permission from the manufacturer, nor to consumable parts, nor to any failure of
lifetime-expired parts. Refer to Repair and Return Policies on page 73.
The warranty is void in the following cases:
● Failure due to incorrect installation
● Failure due to using the incorrect AC power supply
● Failure due to mechanical force applied to the unit
● Failure due to improper handling by the user or an individual not authorized to operate the
equipment
● Failure due to the use of improper spare parts or hardware
● Damage to the software, data, or hard disk due to a PC virus infection
● Corrosion caused by leakage of solvent or samples
● Corrosion of electronic parts caused by highly corrosive atmospheric gas
● Failure due to the disassembly, modification, relocation, or transport after initial installation
● Failure due to the disconnection of main power without taking the specified normal shutdown
procedure
● Failure due to the disregard of safety regulations
● Failure due to not following the maintenance schedule
● Failure due to acts of nature

Gilson is not responsible for any damage caused by improper use of the system, improper
NOTICE maintenance, unauthorized modifications or failure to comply with the procedures
detailed in Gilson documentation.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 75

 Limited Warranty
Fittings and Tubing
TERMS OF USE
● Normal tightening of fittings.
Warranty

● Use of clean solvents and containers (Filter the solvents to avoid presence of fibers and
particles in the pump.)
● Proper positioning, according to the instructions in this guide.

DAMAGES NOT COVERED

● Damaged threads caused by improper tightening.
● Blocked flow paths caused by improper installation or unsuitable mobile phases.
● Bent or cut tubing caused by improper positioning.

Valves
TERMS OF USE
● Normal tightening of fittings.
● Use of clean solvents and containers. (Filter the solvents to avoid presence of fibers and
particles in the pump.)
● Preventative maintenance schedule followed.

DAMAGES NOT COVERED

● Damaged threads or crushed internal parts caused by improper tightening.
● Blocked flow paths caused by improper installation or unsuitable mobile phases.

Pump and Detector

TERMS OF USE
● Use of clean solvents and containers (Filter the solvents to avoid presence of fibers and
particles in the pump.)
● Preventative maintenance schedule followed.

DAMAGES NOT COVERED

● Damaged threads caused by improper installation, replacement, or the use of unsuitable
solvents.
● Blocked flow paths caused by improper installation or unsuitable mobile phases.

76 Warranty | PLC 2050/2250/2500 Purification Systems

 Appendix B

Appendix B | REPLACEMENT PARTS AND

ACCESSORIES

IN THIS CHAPTER:

● Couplers and Adapters | 78

● Detector Parts | 78
● Fittings | 79
● Fraction Collector | 79
● Miscellaneous | 80
● Pump Parts | 81
● Tubing | 83
● Valves | 84

All items listed must only be supplied by Gilson or an agent thereof. Use of alternate parts
CAUTION may lead to improper operation of the system or failure to comply with safety or EMC
regulations.

The manufacturer’s declaration of conformity becomes invalid if the user modifies the
NOTICE
original product or installs additional components.

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 77

 Couplers and Adapters
PART NUMBER DESCRIPTION

21040201 TEE, 1/4-28, 2,4 mm BORE, PEEK, PLC 2050/2250

Replacement Parts and Accessories

4957515 Y ASSY, 5/16-24, PEEK, PLC 2500

49060022 REDUCER 5/16-24 TO 10-32, 1 mm BORE

21040163 FEMALE LUER ADAPTER TO 1/8” FITTING, SS

21040164 BULKHEAD UNION 1/8", SS, PLC 2250/2500

21040165 BULKHEAD UNION 1/16", SS, PLC 2050

21040166 TEE, 1/8”, 2 mm BORE, SS

21040167 TEE, 1/16”, 1 mm BORE, SS

490410678 ADPTR, LUER FEMALE TO 1/4-28 MALE, ETFE

21040202 ADPTR, LUER MALE W/LOCK TO 1/4-28 FEMALE

49041120 QUICK-STOP LUER CHECK VALVE

490410675 ADPTR, LUER MALE W/LOCK TO 1/4-28 MALE

21040203 ADPTR, UNION 1/4-28 MALE TO 1/4-28 MALE

49041131 BACK PRES REG, PEEK, 20 psi

21040207 UNION ETFE FEMALE 1/4-28 TO 1/4-28

21040208 ADPTR, LUER MALE TO 1/4-28 MALE

Detector Parts
PART NUMBER DESCRIPTION

21040113 LAMP DEUTERIUM, PLCA

21040253 LAMP DEUTERIUM, PLCA, V2

21041038 LAMP HALOGEN, PLCA

21040125 FLOW CELL, PLCA, 2.4 mm

21040126 FLOW CELL, PLCA, 1.3 mm

21040127 FLOW CELL, PLCA, 0.3 mm

21040254 DETECTOR TEST CELL FOR SERVICE

78 Replacement Parts and Accessories | PLC 2050/2250/2500 Purification Systems

Fittings
PART NUMBER DESCRIPTION

21040193 ETFE FERRULE FOR 1/8” TUBING (X10)

21040194 PEEK NUT, SHORT, FOR 1/8” TUBING

Replacement Parts and Accessories

21040195 PEEK NUT, LONG, FOR 1/8” TUBING

490410133N ETFE FERRULE FOR 3/16” TUBING

49040132 PEEK NUT FOR 3/16” TUBING

49041019 ETFE PLUG, 1/4-28 FOR GRADIENT VALVE, PLC 2050/2250

21040196 PEEK PLUG 5/16-24 FOR GRADIENT VALVE, PLC 2500

49041122 ETFE FERRULE FOR 1/4” TUBING

49041123 PEEK BLK NUT FOR 1/4” TUBING

21040197 SS NUT FOR 1/16” TUBING (X10)

21040198 SS FERRULE FOR 1/16” TUBING (X10)

21040199 SS NUT FOR 1/8” TUBING (X10)

21040200 SS FERRULE FOR 1/8” TUBING (X10)

490410120 F120, FINGERTIGHT FITTING

Fraction Collector
PART NUMBER DESCRIPTION

21040159 FC PROBE, SS NEEDLE W/ FITTING, PLCA

21040188 NEEDLE, 100 mm TUBES, 11.5 CM, PLCA

21040019 RACK SET 3L, 18x150/180 mm TUBES

21040020 RACK SET 3L, 13x100 mm TUBES

21040021 RACK SET 3L, 16x150 mm TUBES

21040022 RACK SET 3L, 21x150 mm TUBES

21040023 RACK SET 3L, 25x150 mm TUBES

21040024 RACK SET 3L, 28x150 mm TUBES

21040025 RACK SET 3L, 29.5x200 mm TUBES

21040026 RACK SET 3L, 13x73 mm HEMO TUBES

21040191 RACK SET 3L, 12x65 mm TUBES

21040027 RACK FUNNEL, 16 OUTLETS 1/4 OD

FRACTION COLLECTOR CONTINUED ON PAGE 80

Replacement Parts and Accessories | USER’S GUIDE 79

 PART NUMBER DESCRIPTION

21040232 COLLECTION PLATFORM 2 DEEP WELL MCRPLT

21040028 RACK OVERLAYS 18 mm TUBES, 3L

21040029 RACK OVERLAYS 13 mm TUBES, 3L

Replacement Parts and Accessories

21040189 TEST TUBES, 18x150 mm, 25 mL (X 100)

21040190 TEST TUBES, 18x180 mm, 32 mL (X 100)

Miscellaneous
PART NUMBER DESCRIPTION

21040015 STANDALONE COLUMN HOLDER - LC COLUMNS

21040177 ADDITIONAL TIGHTENING CLAMP - LC COLUMNS

21040114 MXNG CHMBR PISTON SEAL, PTFE, PLCA

21040156 MIX CHMBR PISTON (4ML) W/ SEAL, PLCA

21040157 MIX CHMBR PISTON (12ML) W/ SEAL, PLCA

21040158 MIX CHMBR PISTON (16ML) W/ SEAL, PLCA

21040161 MIX CHMBR MOTOR W/ MAG AND CBL, PLCA

21040147 PC FAN, W/ CABLE, PLCA

21040148 BACK FAN, W/ CABLE, PLCA

21040149 AXIAL FAN, 60X60 W/ CABLE, PLCA

21040150 FUSE, 6.3 AMP, H, 250 V, PLCA

21040205 GAS REGULATOR W/FILTER 5µ & MANOMETER

21040128 MAIN PCB, PLCA, CM2008

21040129 PUMP BOARD, PLCA, CPU2008-RP

21040130 FC BOARD, PLCA, CF2008-R

21040131 PUMP INTERFACE BOARD, PLCA, CIP2008

21040132 EXTRNL CONN INTRFACE BD, PLCA, CIC2008

21040133 CPU FOR EXT CONNECTIVITY, PLCA, FLEX

21040134 POWER SUPPLY, PLCA, 24V DC, 300W

21040135 POWER SUPPLY, PLCA, 5V DC, 15W

21040136 CENTRAL PROCESS UNIT W/ TOUCH SCRN, PLCA

21040153 CAMSHAFT OPTO-SENSOR W/ CABLE, PLCA

MISCELLANEOUS CONTINUED ON PAGE 81

80 Replacement Parts and Accessories | PLC 2050/2250/2500 Purification Systems

PART NUMBER DESCRIPTION

21040154 OPTO-SENSOR DISC, PLCA

21040160 SERIAL RS-232 CABLE FOR COM4 USE, PLCA

21040162 STYLUS WITH ADHESIVE HOLDER, PLCA

Replacement Parts and Accessories

21041028 MOTOR SHOCK MOUNT (X4), PLC COLL. MOTOR

21041029 PLC COLLECTOR ARM COVER

21040234 PLC BACK PANEL RIGHT

21040235 PLC COLLECTOR BACK METAL PLATE

21040233 SHIP MTRL PLC, SKID, BOX, FOAMS, ACC BOX

21040206 X-AXIS OPTO-SENSOR W/ CABLE, PLCA

21040243 Y-AXIS OPTO-SENSOR W/ CABLE, PLCA

21041026 X-AXIS BELT FOR PLC COLLECTOR

21041027 Y-AXIS BELT FOR PLC COLLECTOR

21040244 X-AXIS MTR W/ PULLEY, SHCK MNT & CABLE

21040245 Y-AXIS MTR W/ PULLEY, SHCK MNT & CABLE

21040242 BALLAST FOR 1/8” TUBING

21041043 POWER CORD, 110 V

21041044 POWER CORD, 220 V

Pump Parts
PART NUMBER DESCRIPTION

21040103 CHECK VALVE, 10 mm, PLCA

21040104 SEAL, 4.5 mm, PLC 2050

21040105 SEAL, 10 mm, PLC 2250

21040106 SEAL, 14 mm, PLC 2500

21040107 PISTON ASSY, 4.5 X 65 mm, PLC 2050

21040108 PISTON ASSY, 10 X 65 mm, PLC 2250

21040109 PISTON ASSY, 14 X 65 mm, PLC 2500

21040110 CLEANING DISC, 4.5 mm, PLC 2050

21040111 CLEANING DISC, 10 mm, PLC 2250

PUMP PARTS CONTINUED ON PAGE 82

Replacement Parts and Accessories | USER’S GUIDE 81

 PART NUMBER DESCRIPTION

21040112 CLEANING DISC, 14 mm, PLC 2500

21040236 INLET CVH 10mm, 1/8, FLAT, PLC 2050/2250

21040237 INLET CVH 10mm, 3/16, FLAT, PLC 2500

Replacement Parts and Accessories

21040238 OUTLET CVH 10mm, 1/8, CONIC, PLCA

21040250 PUMP HEAD ASSY, PLC 2050

21040251 PUMP HEAD ASSY, PLC 2250

21040152 PUMP HEAD ASSY, PLC 2500

21040155 PRESSURE TRANSDUCER V2, 400 BAR, PLCA

21040174 TOOL FOR SEAL INSERTION, PLCA

Sample Loops
PART NUMBER DESCRIPTION

21040030 SAMPLE LOOP, 1 ML, AUTO, 1/8”

21040031 SAMPLE LOOP, 2 ML, AUTO, 1/8”

21040032 SAMPLE LOOP, 5 ML, AUTO, 1/8”

21040033 SAMPLE LOOP, 10 ML, AUTO, 1/8”

21040034 SAMPLE LOOP, 20 ML, AUTO, 1/8”

21040035 SAMPLE LOOP, 30 ML, AUTO, 1/8”

21040036 SAMPLE LOOP, 40 ML, AUTO, 1/8”

21040037 SAMPLE LOOP, 50 ML, AUTO, 1/8”

21041007 SAMPLE LOOP, 1 ML, MAN, 1/8”

21041008 SAMPLE LOOP, 2 ML, MAN, 1/8”

21041009 SAMPLE LOOP, 5 ML, MAN, 1/8”

21041010 SAMPLE LOOP, 10 ML, MAN, 1/8”

21041011 SAMPLE LOOP, 20 ML, MAN, 1/8”

21041012 SAMPLE LOOP, 30 ML, MAN, 1/8”

21041013 SAMPLE LOOP, 40 ML, MAN, 1/8”

21041014 SAMPLE LOOP, 50 ML, MAN, 1/8”

82 Replacement Parts and Accessories | PLC 2050/2250/2500 Purification Systems

Tubing
PART NUMBER DESCRIPTION

21040101 SOLVENT INLET TBNG ASSY, ETFE, PLC 2050/2250

21040102 SOLVENT INLET TBNG ASSY, PFA, PLC 2500

Replacement Parts and Accessories

21040247 TBNG ASSY, BIN VLV TO HEADS, PLC 2X50

21040246 TBNG ASSY, QUAT VLV TO HEADS, PLC 2X50

21040248 TBNG ASSY, BIN VLV TO HEADS, PLC 2500

21040249 TBNG ASSY, QUAT VLV TO HEADS, PLC 2500

21040115 WASTE OUTLET TBNG, ETFE, PLC 2050/2250

21040116 WASTE OUTLET TBNG, ETFE, PLC 2500

21040137 ETFE TUBING, 1/8" OD, 2.4 mm ID, 1 MTR

21040138 ETFE TUBING, 1/8" OD, 1.6 mm ID, 1 MTR

21040139 PFA TUBING, 3/16" OD, 1/8" ID, 1 MTR

21040140 SS TUBING, 1/8" OD, 2.1 mm ID, 1 MTR

21040141 SS TUBING, 1/8" OD, 1 mm ID, 1 MTR

21040142 SPRING, 20 CM FOR ETFE 1/8" TUBING

21040168 PURGE VLV OUT TBNG, SS FTTNG, PLCA

21040169 SHUT-OFF VLV OUT TBNG, PEEK FTTNG, PLCA

21040170 PTFE TUBING, 1/4” OD, 4.75 mm ID, 1 MTR

21040171 SS TUBING, 1/16” OD, 1 mm ID, 1 MTR

21040117 RT HD, TRNSDCR PRFRM TBNG PLC 2250/2500

21040118 LFT HD, TRNSDCR PRFRM TBNG PLC 2250/2500

21040119 TRNSDCR, MIXER PRFRM TBNG PLC 2250/2500

21040120 MXR TO INJ VLV PRFRM TBNG PLC 2250/2500

21040121 RGHT HD, TRNSDCR PRFRM TBNG PLC 2050

21040122 LFT HD, TRNSDCR PRFRM TBNG PLC 2050

21040123 TRNSDCR, MXR PRFRM TBNG PLC 2050

21040124 MXR TO INJ VLV PRFRM TBNG PLC 2050

21040239 MXR TO VLV ASSY TBNG W/TEE, PLC 2250/2500

Replacement Parts and Accessories | USER’S GUIDE 83

 Valves
PART NUMBER DESCRIPTION

21040143 BINARY GRAD VLV 1/8 W/ CABLE, PLC 2050/2250

Replacement Parts and Accessories

21040144 BINARY GRAD VLV 3/16 W/ CABLE, PLC 2500

21040145 QUAT GRAD VLV 1/8 W/ CABLE, PLC 2050/2250

21040175 QUAT GRAD VLV 3/16 W/ CABLE, PLC 2500

21040146 SOLENOID VLV 1/8, GRAD FORMER, PLC 2050/2250

21040176 SOLENOID VLV 3/16, GRAD FORMR, PLC 2500

21040151 VLV, 6-PORT, 2-POS, 1/8, HEAD RPLCMNT, PLCA

21040173 VLV, 4-PORT, 2-POS, 1/8, HEAD RPLCMNT, PLCA

21041021 CONTROL MODULE FOR AUTOMATIC VICI VALVE

21041022 VLV, 6-PORT, 2-POS, ROTOR SEAL

21040213 VLV, 4-PORT, 2-POS, ROTOR SEAL

21040152 VLV, 3-WAY, 1/8, W/ CABLE, PLCA

21040214 VLV, 3-WAY, 1/8, W/ CABLE, PLCA, V2

21040172 SHUT OFF VLV, INJ VLV OUT, 1/8, PLCA

21041019 NEEDLE, 16GA, SS FOR MAN LOOP INJ VLV

21041020 REBUILT KIT FOR MANU LOOP INJ VLV

84 Replacement Parts and Accessories | PLC 2050/2250/2500 Purification Systems

 Appendix C

Appendix C | MATERIALS

Liquid Contact Materials

The information provided in the following table is accurate to the best of our knowledge and
belief, but it is intended for general information only (classified by alphabetical order).

Liquid Contact Materials

MATERIAL DESCRIPTION

Al2O3 Ceramic Alumina ceramic (Aluminum Oxide or Al2O3) is an excellent electrical insulator and one of
the most widely used advanced ceramic materials. Additionally, it is extremely resistant
to wear and corrosion. Alumina components are used in a wide range of applications such
as electronics, pump components and automotive sensors.

ETFE Ethylene tetrafluoroethylene is the generic name for the material such as Tefzel®. A
fluoropolymer used for sealing surfaces, it is resistant to most chemical attack; however,
some chlorinated chemicals will cause a physical swelling of ETFE tubing.

Fused Silica, SiO2 Glass High purity sand deposits provide the raw material for bulk refractory grade, which is
electric arc melted at extremely high temperatures. Optical and general purpose fused
silica rods and tubing are drawn from a melt made from high purity chemicals. Fiber
optic purity is made by thermal decomposition of high purity gaseous silica containing
chemicals. The glass may be clear or translucent, in which case it is often referred to
as fused quartz. The glass has very high viscosity, and this property allows the glass to
be formed, cooled and annealed without crystallizing. Fused silica glass is a very low
thermal expansion material, and so is extremely thermal shock resistant. The material
is also chemically inert up to moderate temperatures except to hydrofluoric acid, which
dissolves silica. It will devitrify above about 1100°C in the presence of contaminants
such as sodium, phosphorus and vanadium, with the formation of cristobalite crystals
which destroy the properties of the glass. The dielectric properties are stable up through
gigahertz frequencies.

Graphite GFP is a reinforced graphite fiber PTFE material used in moderate to extreme service
Fiber-Reinforced conditions. This material has excellent wear resistance in a wide range of speeds, in
Polytetrafluoroethylene medium pressures and in high temperatures. GFP performs well in water and other liquid
(GFP) solutions and has limited use in vacuum or inert gas applications. Dynamic surfaces in
contact with a seal made from GFP should have a hardness of Rc 40 or higher. GFP is
recommended for applications that require good wear in liquids and humid conditions
at temperatures from -320°F to +500°F (-196°C to +260°C), such as down-hole logging
tools, adhesive, and epoxy dispensing equipment, and chemical and laboratory
equipment. GFP has excellent chemical compatibility. This material is compatible with
most fluids and gases, except some acids, such as sulfuric, nitric and hydrofluoric acids.
LIQUID CONTACT MATERIALS CONTINUED ON PAGE 86

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 85

 Liquid Contact Materials
MATERIAL DESCRIPTION

Hastelloy C This is the material most often recommended for corrosion resistance. This versatile
nickel-chromium molybdenum alloy has excellent resistance to most acids, including
Materials

strong oxidizers such as ferric and cupric chlorides; nitric, formic and acetic acids; wet
chlorine; sea water and brine solutions; and mixtures containing nitric acid or oxidizing
acids with chloride ions.
The best choice for most special applications where HPLC grade stainless cannot be
used, Hastelloy C has excellent resistance to pitting, stress corrosion cracking, and
oxidizing atmospheres up to temperatures well beyond any other standard components
of the chromatographic system.

Kalrez® Kalrez® perfluoroelastomer parts (FFKM) resist over 1,800 different chemicals, while
offering the high temperature stability of PTFE (327°C).
They are used in highly aggressive chemical processing, semiconductor wafer
fabrication, pharmaceutical, oil and gas recovery, and aerospace applications. The
long-term, proven performance of Kalrez® parts can mean less frequent seal changes,
repairs and inspections, increasing process and equipment uptime for greater
productivity and yield.

Ketron® CA30 PEEK Ketron® CA30 PEEK is a polyetheretherketone material with 30% carbon fiber reinforced.
The addition of carbon fibers enhances the compressive strength and stiffness of PEEK,
and dramatically lowers its expansion rate. It offers designers optimum wear resistance
and load carrying capability in a PEEK-based product. This grade provides more thermal
conductivity than unreinforced PEEK, increasing heat dissipation from bearing surfaces
improving bearing life and capability.

PCTFE This material is a homopolymer of chlorotrifluoroethylene which has many of the

properties similar to other fluoropolymers such as PTFE or FEP, but is mechanically
superior in rigidity (does not deform easily), and has very low gas permeability. Its
dimensional stability makes it attractive for use as a component of a structural part where
the high temperature and chemical resistance of fluoropolymers is required. PCTFE
shows high compressive strength and low deformation under load.

PEEK Considered relatively inert and biocompatible, poly-etheretherketone tubing can

withstand temperatures up to 100°C. Under the right circumstances, 0.005”–0.020” (ID)
tubing can be used up to 5000 psi for a limited time, and 0.030 to 3000 psi. Larger
IDs are typically good to 500 psi. These limits will be substantially reduced at elevated
temperatures and in contact with some solvents or acids.
Its mechanical properties allow PEEK to be used instead of stainless in many situations
and in some environments where stainless would be too reactive. However, PEEK can
be somewhat absorptive of solvents and analytes, notably methylene chloride, DMSO,
THF, and high concentrations of sulfuric and nitric acid. This tubing is highly prone to
«kinking», or sealing off, if held in a sharp bend over time.
LIQUID CONTACT MATERIALS CONTINUED ON PAGE 87

86 Materials | PLC 2050/2250/2500 Purification Systems

 Liquid Contact Materials
MATERIAL DESCRIPTION

PFA Perfluoroalkoxy is a fluorocarbon with chemical and mechanical properties similar to

FEP. More rigid than either PTFE or FEP. FEP (fluorinated ethylene propylene) is another

Materials
member of the fluorocarbon family with similar chemical properties. It is generally
more rigid than PTFE, with somewhat increased tensile strength. It is typically more
transparent than PTFE, slightly less porous, and less permeable to oxygen. FEP is not as
subject to compressive creep at room temperature as PTFE, and because of its slightly
higher coefficient of friction is easier to retain in a compression fitting.

PTFE Polytetrafluoroethylene is the generic name for the class of materials such as Teflon®.
It offers superior chemical resistance but is limited in pressure and temperature
capabilities. Because it’s so easy to handle, it is often used in low pressure situations
where stainless steel might cause adsorption. PTFE tubing is relatively porous, and
compounds of low molecular weight can diffuse through the tubing wall.

Ruby / Sapphire Synthetic rubies and sapphires are single-crystal aluminum oxides, practically pure for
the sapphire (+99,99% Al2O3). The color of the ruby is produced by adding a few ppm
(parts per million) of chromium oxide (CrO3). Synthetic rubies and sapphires have a
hexagonal-rhombic crystal structure, density of 3.99 g/cm³ and a water absorption
coefficient of 0%.
The principal properties of synthetic rubies and sapphires include a hardness and high
mechanical strength, excellent resistance to wear, very low friction coefficient, chemically
inert, good thermal conductivity, ideal electrical insulation.
LIQUID CONTACT MATERIALS CONTINUED ON PAGE 88

Materials | USER’S GUIDE 87

 Liquid Contact Materials
MATERIAL DESCRIPTION

Stainless Steel, Stainless Steel 316 is a chromium-nickel based steel that possesses increased levels
Type 316/316L of resistance against several substances, due to the addition of molybdenum in its
Materials

composition. The molybdenum allows SS 316 to be more resistance to corrosion

overall, with specific resistance against chlorine pitting. SS 316 is also found to be more
applicable in areas with high or elevated temperatures compared to other stainless
steels. It has more heat resistance than Type 304 but otherwise possesses many of the
same general characteristics.
316’s corrosion resistance is especially effective against chemicals, such as those used
in the paper and textile industries. The metal finds other common applications in food
processing equipment, chemical processing, nuts and bolts, and medical implants.
While the metal is pliable under hot and cold-working techniques, it cannot be work
hardened with heat treatment and in general is considered to have lower weldability
compared to the 304 type stainless steels. However, it is still preferred over many other
stainless steel grades in terms of workability.
Stainless Steel 316L was designed to have a much lower carbon content than its 316
counterpart. As such, 316L is useful in areas where the avoidance of carbon precipitation
is desired. The metal is commonly applied in weldments, where its particular carbon
content combined with welding guarantees maximum resistance against general
corrosion, and also in heavy gauge components.
316L is considered to be more resistant to oxidation than type 316, especially in warm
marine environments. Once again, its low levels of carbon protect it against carbon
precipitation. The metal also shows resistance in extremely low temperatures, even down
to cryogenic levels. In regard to heat, 316L shows better resistance to creep, stress to
rupture and overall strength than other stainless-steel grades.
Many of the same work practices effective on type 316 may also be employed on 316L,
including weldability and work hardening through cold working. In addition, 316 does not
require post-work annealing to maximize its corrosion resistance, however annealing may
be utilized in certain situations.

Zirconium Oxide The zirconium oxide or zirconia (ZrO2) has a tetragonal crystal structure with a grain size
not exceeding 0.50 microns, density greater than 6,00 g/cm³, and a Vickers hardness
value of about 1200.
The principal properties of zirconia ZrO2 include a high mechanical strength, fracture
toughness, extreme hardness, compressive strength, low thermal conductivity,
excellent resistance to corrosion and wear, excellent tribological properties and good
biocompatibility.

Trademark Description References

Al2O3 ceramic description provided by Elan Technology (www.elantechnology.com)
ETFE, Hastelloy C, PEEK, PFA, and PTFE descriptions provided by Valco Instruments Co. Inc. (www.vici.com)
Fused Silica, SiO2 Glass description provided by Accuratus Corporation (www.accuratus.com)
GFP description provided by Bal Seal Engineering, Inc. (www.balseal.com)
Kalrez® descriptions provided by DuPont (www.dupont.com)
Ketron® CA30 PEEK description provided by Quadrant (www.quadrantplastics.com)
PCTFE description provided by Fluorotherm (www.fluorotherm.com)
Stainless Steel, Type 316 / 316L descriptions provided by Stardust Impex (www.stardustimpex.com)
Ruby/Sapphire and Zirconium Oxide descriptions provided by Ceramaret SA (www.ceramaret.ch)
88 Materials | PLC 2050/2250/2500 Purification Systems
 Appendix D

Appendix D | PLC WITH GX-241 Liquid Handler

CONFIGURATION

IN THIS CHAPTER:

● Unpack the System | 90

● System Setup | 90
● Connect the Transfer Tubing | 90
● Control Software | 90
● Installation | 91
● Plumbing Connections | 92
● Transfer Tubing Connection | 94
● Rear Panel Connections | 94
● Start Up | 96
● Operational Description of the VERITY 4020 Syringe Pump | 97

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 89

 Unpack the System
The PLC Purification System, GX-241 Liquid Handler, and VERITY® 4020 Single Syringe Pump
are shipped separately.
Upon receipt of each instrument, inspect the exterior of all shipping boxes. All boxes should
arrive unopened and undamaged. Report any damage to the shipping carrier and Gilson as soon
PLC with GX-241 Liquid Handler Configuration

as possible after receipt. Keep packing materials in case the instrument needs to be shipped in
the future.

System Setup
Follow the instructions to set up the GX-241 Liquid Handler and VERITY® 4020 Single Syringe Pump
as described in each instrument’s user’s guide.

Do not connect the transfer tubing as instructed. This is a system connection that will be described
NOTE
later in this section.

Connect the Transfer Tubing

Connect the transfer tubing as described in Transfer Tubing Connection on page 94.

Control Software
Control of the PLC Purification System with the GX-241 as autosampler with
VERITY 4020 Syringe Pump is via GLIDER Software accessed via the touchscreen of the
PLC Purification System.

90 PLC with GX-241 Liquid Handler Configuration | PLC 2050/2250/2500 Purification Systems
Installation
Install the GX-241 and the VERITY 4020 Syringe Pump as described in each instrument’s
user’s guide. Complete instructions for each step are included in the ‘Installation’ chapter. The
instrument and its components should be set up in the order shown below.

PLC with GX-241 Liquid Handler Configuration

Figure 71
PLC Purification System with GX-241 Liquid Handler

1. Set up the GX-241.

2. Place the GX-241 to the left of the
PLC Purification System.
3. Place the 3 x 100 mL solvent reservoir
rack (PN SPL-2334-HDW), which must
be installed adjacent to the rinse station.
4. Set up the VERITY 4020 Syringe Pump.
5. Place the VERITY 4020 Syringe Pump to
the left of the GX-241.
6. Install the syringe. The syringe is ordered
separately, according to the PLC sample
loop size. Refer to the table for part
numbers. Figure 72
Location of 3 x 100 mL Solvent Reservoir
Syringes
Part
Description
Number

25025344 Syringe 5 mL

25025345 Syringe 10 mL

25025346 Syringe 25 mL

PLC with GX-241 Liquid Handler Configuration | USER’S GUIDE 91

 Plumbing Connections
Make all connections as described in each instrument user’s guide, except the transfer tubing
connection, as this is different with the PLC Purification System.
PLC with GX-241 Liquid Handler Configuration

Embedded
D
Detector

K 1
2
C F 6

3
5
E 4

B
A
G

Embedded Pump
System

L
H
J

Reservoir VERITY® 4020 GX-241 Liquid Handler PLC Purification System

Single Syringe
Pump

Figure 73
Plumbing Diagram for the PLC Purification System with GX-241 Liquid Handler
and VERITY® 4020 Single Syringe Pump

PLC Purification System with GX-241 Liquid Handler Plumbing Connections

Connection Tubing Fittings

A Solvent inlet tubing assembly 20 µL filter

FROM Reservoir
(PN 4957226)
• 3 mm (OD), 2 mm (ID)
(PN 499484021)
TO Syringe pump inlet PEEK

Transfer tubing
FROM Syringe pump outlet • 5.5 mL for 5 mL syringe
(PN 499671112)
B • 10.5 mL for 10 mL syringe PEEK
Port 2 (PN 499474103)
TO Automated sample loop • 30 mL for 25 mL syringe
injection valve (PN 499483602)

PLC PURIFICATION SYSTEM WITH GX-241 LIQUID HANDLER PLUMBING CONNECTIONS CONTINUED ON PAGE 93

92 PLC with GX-241 Liquid Handler Configuration | PLC 2050/2250/2500 Purification Systems
 PLC Purification System with GX-241 Liquid Handler Plumbing Connections
Connection Tubing Fittings

FROM GX-241 1/4”-28 PEEK

PLC with GX-241 Liquid Handler Configuration

C Port 1 1/16" (OD) PTFE probe tubing
TO Automated sample loop 10-32 SS
injection valve

FROM Column Varies, based on

Varies, based on PLC model and
D PLC model and
connected column
TO Detector connected column

Port 5
FROM Automated sample loop Varies, based on
Varies, based on PLC model and
E injection valve PLC model and
connected column
connected column
TO Column

Port 3 Sample loop tubing

FROM Automated sample loop
injection valve • 1 mL (PN 21040030)

F • 2 mL (PN 21040031) 5/16”-24 SS

Port 6 1/8”
• 5 mL (PN 21040032)
TO Automated sample loop (OD)
• 10 mL (PN 21040033)
injection valve
• 20 mL (PN 21040034)

Embedded pump system

FROM
(mixing chamber)
G 1/8” (OD), SS 5/16”-24 SS
Port 4
TO Automated sample loop
injection valve

FROM Rinse station Installed

Rinse station assembly
H
(PN 26054000)
TO Waste N/A

FROM GX-241 locator plate

Tygon® waste tubing
J N/A
(PN 470331206)
TO Waste

FROM Syringe pump vent N/A

Vent tubing
L
(PN F4420577)
TO Waste N/A

K Spiral wrap to contain probe tubing

PLC with GX-241 Liquid Handler Configuration | USER’S GUIDE 93

 Transfer Tubing Connection
The transfer tubing is routed from the syringe pump to the outlet of the automatic loop injection
valve of the PLC Purification System. Refer to Plumbing Connections on page 92.
The transfer tubing is ordered separately, according to the syringe size. Refer to the table below
for part numbers.
PLC with GX-241 Liquid Handler Configuration

Transfer Tubing
Part Number Syringe Size Description

499671112 5 mL Transfer tubing 5.5 mL

499474103 10 mL Transfer tubing 10.5 mL

499483602 25 mL Transfer tubing 30 mL

To install the transfer tubing:

1. Connect one end with 1/4”-28 fitting to the transfer tubing side of the valve of the
VERITY 4020 Syringe Pump (right-hand port), and finger-tighten the nut.
2. Connect the other end to the outlet of the shut-off valve, connected to the waste outlet
(port 2) of the automatic loop injection valve of the PLC Purification System and finger-
tighten the nut.

Rear Panel Connections

Diagram
The following diagram provides a general overview of the connections described in this section.

Right Left
Side Side

OR
C

E
3
2
+1 0 9

4 5 6
4 5 6
8
7

A B 3
2
7 F
8
109
G
VERITY® 4020 GX-241 PLC
Single Syringe Liquid Handler Purification
Pump System

Figure 74
Electrical Diagram for the PLC Purification System with GX-241 Liquid Handler
and VERITY® 4020 Single Syringe Pump

94 PLC with GX-241 Liquid Handler Configuration | PLC 2050/2250/2500 Purification Systems
 GX-241, VERITY 4020 Syringe Pump, and
PLC Purification System Electrical Connections
Connection Description

A FROM VERITY 4020 Syringe Pump External power supply and power cord (appropriate

PLC with GX-241 Liquid Handler Configuration

for line voltage)
TO External power supply, and then to AC
power source

B FROM GX-241 External Power Supply and Power Cord (appropriate

for line voltage)
TO External power supply, and then to AC
power source

C FROM VERITY 4020 Syringe Pump USB cable, B-type end (square)

TO PLC Purification System USB cable, A-type end (flat)

D FROM Z-arm Z-arm cable*

*Ensure power is off before connecting Z-arm.
TO GX-241

E FROM GX-241 RS-232 cable

TO PLC Purification System

F FROM PLC Purification System Power cord

TO Power outlet

G Unit ID

GX-241
The GX-241 is shipped configured for RS-232 communication with a unit ID of 8, which is the
supported configuration in the PLC Purification System.
To make the RS-232 connection between the GX-241 Liquid handler and the PLC Purification
System, use the RS-232 cable supplied.

1. Attach the male end of the RS-232 cable to the RS-232 port located on the rear panel of the
liquid handler. Tighten the retaining screws.
2. Attach the female end of the RS-232 cable to the RS-232 serial communication port (COM4)
located on the right panel of the PLC system. Tighten the retaining screws.

UNIT ID
At the factory, the unit ID on the liquid handler is set to 30. The selector on the rear panel has
the arrow aligned with 0. The unit ID is 30 plus the selected number. There is no need to change
this number.

Z-ARM CONNECTION
Connect the cable from the Z-arm to the Z-ARM port on the rear panel and then use a small,
flat-blade screwdriver to tighten the screws to secure it

PLC with GX-241 Liquid Handler Configuration | USER’S GUIDE 95

 VERITY 4020 Syringe Pump
The VERITY 4020 Syringe Pump communicates with GLIDER via USB.
To make the USB connection between the VERITY 4020 Syringe Pump and the
PLC Purification System, use the USB cable supplied in the accessory kit. Use the end with the
A-type (flat) connector to connect to a USB port of the PLC Purification System, and use the end
PLC with GX-241 Liquid Handler Configuration

with the B-type (square) connector to connect to the VERITY 4020 Syringe Pump.

Power Connections
Use the power cord on the external power supply to make the connection between the power
receptacle on the GX-241 Liquid Handler and the external power supply.
The connection from the external power supply to the GX-241 Liquid Handler uses a connector
with a locking collar. Check the alignment of the pins and then push in until it clicks and locks in
place. To disconnect, pull back on the locking collar and then disconnect the cable from the rear
panel of the GX-241 Liquid Handler.
Locate the appropriate power cord for the line voltage and then make the connection between
the external power supply and the AC power source.
Repeat the previous operation for the power connection of the VERITY 4020 Syringe Pump and
its external power supply.

Start Up
Follow all prior instructions to make all rear panel and plumbing connections.
To start the GX-241 Liquid Handler and the VERITY 4020 Syringe Pump:

1. Power on the external supply, and then power on the GX-241 Liquid Handler, using the
power switch on the rear panel.
● The green power indicator light on the front panel illuminates.
● The two-digit front panel display indicates 0 (no error).
2. Power on the VERITY 4020 Syringe Pump, using the power switch on the side panel.
● The green power indicator light on the front panel illuminates.
● The syringe pump initializes. It stops with the valve set to the outlet (transfer tubing)
position.
3. Power on the PLC Purification System and wait for GLIDER to start.

96 PLC with GX-241 Liquid Handler Configuration | PLC 2050/2250/2500 Purification Systems
Operational Description of the VERITY 4020 Syringe Pump
The VERITY 4020 Syringe Pump has a single syringe and valve. The instrument only dispenses
to the GX-241 probe via the automatic loop injection valve of the PLC Purification System.

PLC with GX-241 Liquid Handler Configuration

Figure 76 Figure 77 Figure 75
Aspirating from Aspirating from Probe Dispensing
Reservoir

It is impossible to dispense to the reservoir.

When aspirating from the probe, it is important to take into account the volume of transfer tubing
NOTE connecting the pump outlet to the probe. The liquid aspirated from the probe must never enter the
valve. The maximum volume of liquid that can be aspirated from the probe is limited to the volume
of the syringe, or the volume of the transfer tubing, whichever is smaller.

PLC with GX-241 Liquid Handler Configuration | USER’S GUIDE 97

 Appendix E

Appendix E | PLC WITH VERITY 1920 MS

CONFIGURATION

IN THIS CHAPTER:

● Unpack the System | 100

● Control Software | 100
● System Setup | 100
● Electrical Connections | 100
● Exhaust Connections | 101
● Plumbing Connections | 102
● Start Up | 104

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 99

 Unpack the System
The components of the PLC Purification System and VERITY 1920 MS system are shipped
separately. Follow any unpacking instructions provided with each component when unpacking
the system.
Report any damage to the shipping carrier and Gilson as soon as possible after receipt. Keep
PLC with VERITY 1920 MS Configuration

packing materials in case the component needs to be shipped in the future.

Control Software
Control of the PLC Purification System with VERITY 1920 MS, VERITY 3011 Pump and MRA Splitter
is via GLIDER Software accessed via the touchscreen of the PLC Purification System.

System Setup
Install each system component per the instructions provided in each instrument’s user’s guide
and in the site requirements document.
Install the rotary vane pump underneath the bench or fume hood when possible. If a
diaphragm pump is used, place it on top of the rotary vane pump, at the back. The MRA Splitter
can be stacked on top of the VERITY 3011 Pump.
Position the chromatographic column (HPLC or CPC) between the PLC Purification System and
VERITY 1920 MS system.

Figure 78
Stacking Diagram

Electrical Connections
The rear panel of the VERITY 1920 MS includes connections for power and nitrogen gas. The front
panel includes vacuum exhaust, house exhaust, waste outlet, and a USB communication port.
The rear panel of the VERITY 3011 Pump includes connections for power and communication.
The USB port is used for the communication with the PLC Purification System.
The rear panel of the MRA Splitter includes connections for power and communication. The
RS-232 port is used for the communication with the PLC Purification System.

100 PLC with VERITY 1920 MS Configuration | PLC 2050/2250/2500 Purification Systems
Communication Connections
USB

VERITY 1920 MS
The USB port on the VERITY 1920 MS is used for remote control with GLIDER.

PLC with VERITY 1920 MS Configuration

To make the USB connection between the VERITY 1920 MS and the PLC Purification System, use
the USB cable supplied. Connect the B-type (square) end to the USB port located on the front panel
of the VERITY 1920 MS, and then connect the A-type (flat) end to the PLC Purification System.

VERITY 3011 Pump

The USB port on the VERITY 3011 Pump is used for remote control with GLIDER.
To make the USB connection between the VERITY 3011 Pump and the PLC Purification System, use
the USB cable supplied. Connect the B-type (square) end to the USB port located on the rear panel
of the VERITY 3011 Pump, and then connect the A-type (flat) end to the PLC Purification System.

RS-232

MRA Splitter
The RS-232 port on the MRA Splitter is used for remote control with GLIDER.
To make the RS-232 connection between the MRA Splitter and the PLC Purification System,
use the RS-232 gender changer and the USB/RS-232 converter cable supplied. Connect the
gender changer to the RS-232 port located on the rear panel of the MRA Splitter and tighten the
retaining screws. Connect the male RS-232 end of the converter cable to the gender changer
and tighten the retaining screws. Connect the USB A-type end of the converter cable to the
PLC Purification System.

Power Connections
Do not turn on the instruments until instructed.

Exhaust Connections
Vacuum Exhaust
The vacuum exhaust tubing is already connected inside the VERITY 1920 MS and goes out
from the front left of the instrument. This large, spiral-supported 1.3" OD- 1" ID tubing must be
connected to the rotary vane pump (clamp connection).

House Exhaust
The house exhaust tubing is already connected inside the VERITY 1920 MS and goes out from
the front right of the instrument. This spiral-supported 1" OD–3/4" ID tubing must be connected
to the Ion Source Exhaust Trap, the 4L bottle with cap and adapters (barbed connection).

PLC with VERITY 1920 MS Configuration | USER’S GUIDE 101

 Plumbing Connections
After setting up the system, make the plumbing connections as described in each instrument’s
user’s guide.
The flow rates of the PLC Purification System are too high for micro-spray operation with the
VERITY 1920 MS; therefore, the analysis of samples with a PLC Purification System can only be
PLC with VERITY 1920 MS Configuration

carried out using a splitter valve set up with the correct split ratio.
The outlet from the column is directed to the HPLC stream inlet of the MRA Splitter, where
the fluid stream is split between the integrated UV detector and the VERITY 1920 MS. The
HPLC stream outlet of the MRA Splitter is redirected to the UV detector bulkhead union of the
PLC Purification System.
The VERITY 3011 Pump is used as the make-up pump and its outlet is connected to the MS stream
inlet of the MRA Splitter. The VERITY 1920 ACTIVE SPLITTER KIT (PN 18005005) includes all
necessary tubing to connect the MS stream outlet of the MRA Splitter to the VERITY 1920 MS.
Use 1 m of the red PEEK tubing (PN 499500591) to connect the MRA Splitter to the flow injection
analysis (FIA) injection valve and use 0.5 m of the same tubing to connect the FIA injection valve
to the ion source.
The waste outlet tubing of the VERITY 1920 MS is already connected inside and goes out from
the front right of the instrument. Place the end of this Tygon® 3/8”OD-1/4” ID tubing into suitable
waste receptacle.
The following diagram shows and the table explains the plumbing connections between system
components.

3
2 5
A B
1 F E
4
D

Make Up MRA
Solvent Splitter

C 6
G
7
H
CPC
PLC Purification System

VERITY 1920 MS
VERITY 3011 Pump

Figure 79
System Plumbing Connections

102 PLC with VERITY 1920 MS Configuration | PLC 2050/2250/2500 Purification Systems
 System Plumbing Connections
ID DESCRIPTION

A FIA injection valve

PLC with VERITY 1920 MS Configuration

B Ion source

C Purge valve of VERITY 3011 Pump (make up pump)

D Automatic loop injection valve

E Automatic backflush ASC/DSC valve

F Detector bulkhead union

G CPC ASC inlet / DSC outlet

H CPC DSC inlet / ASC outlet

1 MRA Splitter to FIA injection valve (port 2)

PEEK tubing, red, 1/16" (OD), 0.005"/0.125 mm (ID), 1 m

2 FIA injection valve (port 3) to ion source

PEEK tubing, red, 1/16" (OD), 0.005"/0.125 mm (ID), 0.5 m

3 MRA Splitter to integrated UV/VIS detector or DAD in the PLC Purification System
Cut the tubing to the appropriate length at installation:
PEEK tubing, green, 1/16" (OD), 0.030"/0.75mm (ID), 50 ft/15 m (included in the semi-prep tubing kit
PN 18005100)
or
PEEK tubing, natural, 1/16" (OD), 0.040"/1.0 mm (ID), 50 ft/15 m (included in the prep tubing kit
PN 18005101)
This tubing corresponds to the “Tubing Kit” in GLIDER.

4 VERITY 3011 Pump (make up pump) to MRA Splitter

PEEK tubing, green, 1/16" (OD), 0.03"/0.75 mm (ID)

5 Column (through backflush ASC/DSC valve) to MRA Splitter

PEEK tubing, green, 1/16" (OD), 0.03"/0.75 mm (ID)

6 /7 Flow to CPC column

PEEK tubing, green, 1/16" (OD), 0.03"/0.75 mm (ID)

PLC with VERITY 1920 MS Configuration | USER’S GUIDE 103

 Start Up
Follow all prior instructions to make all rear panel and plumbing connections.
Power on all system components except the PLC Purification System. Allow each component to
initialize and then power on the PLC Purification System and wait for GLIDER to load.
If the PLC Purification System and the different peripherals are properly configured and the
PLC with VERITY 1920 MS Configuration

communication established, there is no communication error and the system is ready to operate.
For more information about how to control a VERITY 1920 MS and PLC Purification System using
GLIDER, refer to the instructions document on the documentation USB drive shipped with the
system.

104 PLC with VERITY 1920 MS Configuration | PLC 2050/2250/2500 Purification Systems
 Appendix F

Appendix F | PLC WITH INTEGRATED ELSD

IN THIS CHAPTER:

● Technical Specifications | 106

● Description | 107
● Make the Rear Panel Connections | 108
● Install the Nebulizer and Nebulization Chamber | 111
● Front Panel Connections | 112
● Install the Chamber Shield | 114
● Operation | 114
● Split Flow Rates and Ratios | 117
● Maintenance | 118
● Troubleshooting | 119
● Clean the Nebulizer | 120
● Decontaminate the Nebulizer | 121

PLC 2050/2250/2500 Purification Systems | USER’S GUIDE 105

 Technical Specifications
Please be aware of the following before operating the instrument.

PLC Purification System with Integrated ELS Detector

Specification Definition
PLC with Integrated ELSD

Communication USB (internal)

Control GLIDER Software installed on the integrated touchscreen PC

Data rate Up to 30 Hz

Detection Photodiode with high sensitivity

Filter Moving average

Gain setting Dynamic gain management: SAGA (SEDEX Automated Gain Adjustment)

Gas requirements* Specification Definition

*For oil-free, dry and filtered gas,
nitrogen, or air. Pressure 2–4.5 bar (29–67 psi)

Purity Gas purity has negligible impact on ELS detector performance

Tubing Polyamide (PA), 6 mm (OD)

Fitting Stainless steel (SS), 6 mm with push-lock fitting

Light source Selected high efficiency blue LED (470 mm) with elapsed-time counter

Nebulizer* Specification Definition

*Model SEDEX FP™ (Flash/Prep)
Flow rate range 100 µL/min to 5 mL/min

Temperature range Ambient to 100°C

Weight* 5 kg (11 lbs.)

*In addition to a standard
PLC Purification System

106 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Description
The low temperature, evaporative light-scattering (ELS) detector integrated in the
PLC Purification System detects compounds in the eluent of the liquid purification chromatography
system. It is capable of monitoring eluent flow rates from 100 μL/min. to 5 mL/min.
ELS detection (ELSD) is a nearly universal technique that can detect any analyte less volatile

PLC with Integrated ELSD

than the mobile phase. Unlike other modes of detection, such as UV, it is not dependent on the
absorption of radiation, nor do the absorption characteristics of the solvent affect detection.
Thus, solvents which absorb UV radiation can be used. As the solvent is completely evaporated,
a gradient can be performed to optimize the separation.
ELSD is a three step process:

1. Nebulization is achieved with a venture nebulizer.

2. Evaporation is achieved with a heated tube. This is an adjustable parameter.
3. Light diffusion detection is measured with a photodiode.

Liquid
Nebulizer
Inlet Large
Black
Nut
Shield
Holder
Small Nebulization
Gas
Black Chamber
Inlet
Nut
Shield
Holder
Nebulization
Chamber Nebulizing
Gas
Inclined
Tubing
Support

Siphon Drain Tube

Figure 80 Figure 81
ELSD Components ELSD Installed on PLC

Shield

Figure 82
Shield Installed over ELSD Components

PLC with Integrated ELSD | USER’S GUIDE 107

 Make the Rear Panel Connections
Rear Panel Diagram
The rear panel provides connections for a gas inlet and exhaust outlet. The exhaust outlet
evacuates gaseous mobile phase vapor and solute particles from the detector.
PLC with Integrated ELSD

Figure 83
Rear Panel Components Diagram for PLC with ELSD

Rear Panel Components

Part Description

A Gas

B Exhaust

108 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Connect the Gas Supply
A supply of filtered, oil-free, dry, and clean gas (nitrogen or air) is required to operate the
ELS detector.

Highly purified gas is not required to operate the ELS detector; however, a good quality of gas
NOTICE is mandatory for high performance detection. The gas should be free from particles (dust) and

PLC with Integrated ELSD

from oil.

Fire and explosion hazard.

Do not use a combustible gas with combustible solvents or analytes.
WARNING
Inspect the supplied tubing for damage after installation and before use. Any damage to
tubing could permit gas to leak into the laboratory.

The gas supply must include a pressure gauge that is stable and regulated by an external
manometer. The ELS detector is typically operated with a gas pressure of 2 bar (29 psi) and
gas consumption of 3 L/min. A gas regulator (sold separately, PN 21040205) with a 5 µm filter,
manometer, and fittings is available for purchase.
To connect the PLC Purification System to the gas supply:

1. Connect a suitable length of clean, 6 mm (OD) tubing from the gas regulator outlet to the
gas inlet (Port A).
2. Connect a suitable length of clean, 6 mm (OD) tubing from the gas source to the gas
regulator inlet.
3. Insert the tube into the fitting until it reaches the back, and then pull the tube to check
engagement of the grab.

Ensure that the gas pressure supplied to the detector is less than 4.5 bar (67 psi). If the
NOTICE pressure increases above 4.5 bar, the pressure sensor may be damaged (not covered by
the warranty).

PLC with Integrated ELSD | USER’S GUIDE 109

 Vent the Exhaust Line
The carrier gas containing volatilized mobile phase and analytes exits the detector through the
black exhaust tube. This tube can be cut and should be directed into a fume hood or exhaust vent.

Vent to Fume Hood

PLC with Integrated ELSD

Ensure that the fume hood draws gas from the detector, i.e., there is positive pressure between
the detector and the fume hood.

Use a moderate vacuum to avoid turbulence in the nebulization chamber siphon or to prevent
liquid from spilling into the evaporation tube.
Avoid loops or bends in the black exhaust tubing to avoid condensation traps that can result
NOTICE
in poor measurement results.
Negative pressure between the fume hood and ELS detector can introduce contaminants
from the fume hood into the ELS detector.

Connect the exhaust tubing to the fume hood. If necessary, use the two adjustable, self-adhesive
clips supplied to guide the vent tube.

Ensure that the vent tube does not become

blocked, bent, or restricted from the detector to
the fume hood.
WARNING
Avoid long tube installations in an upward
direction, which can create condensation that
flows back into the detector.

If an extension line is required because the supplied

NOTE tubing is not long enough, a suitable length of 3/4” (ID)
tubing can be fitted over the exhaust tubing.

Figure 84
Exhaust Tubing and Self-Adhesive Clips

110 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Install the Nebulizer and Nebulization Chamber
The nebulizer and the nebulization chamber are fragile. Unpack and handle them with care.
NOTICE
Ensure that each component is clean before installation.

To install the nebulizer and nebulization chamber:

PLC with Integrated ELSD

1. Remove the yellow cap from the evaporation tube.
2. Remove the paraffin film from the nebulization chamber.
3. Position the nebulization chamber onto the stainless steel (SS) evaporation tube with the
siphon directed down. The chamber should be pushed as far as possible.
4. Rest the nebulization chamber on its inclined support, and then tighten the small black nut
at the bottom.
5. Place the nebulizer on the top of the nebulization chamber, and then tighten the large black
nut with the gas inlet fitting directed down.

Evaporation Evaporation
Tube Cap Tube
Small
Black
Nut
Inclined
Support

Figure 85
Removing the Evaporation Tube Cap Figure 86
Positioning the Nebulization Chamber

6. Fill the siphon of the nebulization chamber with the mobile phase that will be used for the
separation. The liquid should fill the bend in the siphon, but not pool in the evaporation
tube. Ensure that no liquid leak could affect the detector performance or create laboratory
pollution.

If using a volatile solvent (e.g., hexane or

NOTICE
dichloromethane), use water to fill the siphon.

Figure 87
Fill Area for Siphon Overflow

PLC with Integrated ELSD | USER’S GUIDE 111

 Front Panel Connections
Connect the Siphon Overflow
1. Connect the supplied drain tube assembly
to the end of the siphon tube, using the
PLC with Integrated ELSD

red, tapered hose connector with PTFE

seal.
2. Lead the drain tube to waste.

Drain Tube Siphon

Figure 88
Siphon Overflow Connections

Ensure that the waste container is directly below the siphon outlet and that there are no
loops or bends in the drain tubing.
Affix the drain tubing to the inlet of the waste container, so that the end of the tubing
never submerges into the liquid.
NOTICE
If the solvent you are using is not compatible with the drain tube, such as
tetrahydrofuran (THF), use a piece of PTFE tubing or any material compatible with your
solvent in its place. When using this type of tubing, which is generally more rigid, ensure
that it is safely installed, so that it will not damage the nebulization chamber (glassware).

112 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Connect the Nebulization Gas Supply
Use the short, 4 mm (OD) gas tubing supplied to connect the gas outlet on the front panel to the
gas inlet fitting on the nebulizer.

PLC with Integrated ELSD

Gas
Inlet
Fitting

Gas
Tubing

Gas
Outlet
Fitting

Figure 89
Nebulization Gas Supply Connections

Connect the Splitter Tubing

Shut-
Since ELS detection is a destructive technique, the Off
10‑32
PLC Purification System includes a splitter to divert a Union
Valve
fraction of the purification flow to the ELS detector while
the remainder flows to the UV-VIS detector, and then on to
the fraction collector.
Use the supplied, 1/16” (OD) PEEK tubing to connect the
splitter outlet to the ELS detector nebulizer, taking care not
to kink the tubing.

1. Screw the 1/16” (OD) PEEK fitting of the nebulizer inlet

tubing to the black, 10-32 PEEK union on the splitter
tubing until it is fingertight.
2. Unscrew the plug from the PEEK shut-off valve located
on the top-left side of the system.
3. Screw the other end of the splitter tubing with PEEK
Figure 90
fittings into the shut-off valve outlet until it is fingertight.
Splitter Tubing Connections

PLC with Integrated ELSD | USER’S GUIDE 113

 Install the Chamber Shield
The chamber shield encloses the nebulization chamber and is held in place by two black plastic
holders in front. The chamber shield is designed to minimize glassware breakage and prevent
tubing deterioration. Take care not to damage the splitter tubing, nebulizer, and nebulization
chamber, and then slide the shield diagonally downward.
PLC with Integrated ELSD

To install the chamber shield slide the chamber shield into the exposed notches of the two shield
holders.

Shield
Holders

Figure 91
Installing the Chamber Shield

Operation
Start Up
Make all rear and front panel connections before start up.
To use the ELS detector:

1. Open the gas source.

2. Unlock the regulating device of the gas regulator by lifting it to be able to adjust the gas
pressure supplied to the system.
3. Turn the regulating device clockwise (+) to set the gas pressure to 2 bar (29 psi) on the
manometer.
4. Once the gas pressure is adjusted, lock the regulating device by pressing it.
5. Open the split way by switching the quarter-turn shut-off valve (small handle turned up),
that allows to divert a minor fraction of the purification flow to the ELS detector.

114 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

6. Ensure that the overflow siphon for the nebulization chamber is filled with the mobile phase
that will be used for the separation. If necessary, pump a few milliliters of solvent through the
instrument to fill the siphon.
Ensure that the waste container is directly below the siphon outlet and that there are no
loops or bends in the drain tubing.

PLC with Integrated ELSD

Affix the drain tubing to the inlet of the waste container, so that the end of the tubing is
never submerged in the liquid.
NOTICE Drain tubing that is bent or immersed in the waste container will create pressure
fluctuations in the ELS detector and result in poor measurements.
The liquid level in the siphon must be stable and should be equal at both sides. If the
vacuum is too strong, liquid is drawn into the evaporation tube or generate air bubbles
from the drain tube and both resulting in bad measurement results.

7. Power on the PLC Purification System and wait for GLIDER to load.
● If the PLC Purification System and the ELS detector are properly configured and
communication is established, a second detector cell with two green dots appears on
the software screen.
● The ELS detector warms to reach the default temperature selected in the
‘System Configuration’ window of GLIDER.

PLC with Integrated ELSD | USER’S GUIDE 115

 Select the Optimum Temperature
There are two factors that should be taken into account when selecting the optimum temperature
for the detector:
● Increasing temperature will optimize the evaporation of the mobile phase.
● Decreasing temperature will minimize the decomposition of thermally labile compounds and
PLC with Integrated ELSD

the volatilization of semi-volatile compounds.

A very reasonable start is to set the temperature to 60°C if an aqueous mobile phase is used
and 40°C if an organic mobile phase is used (these temperatures are suggested for a flow rate
of 1 mL/min). At higher flow rates, more elevated temperatures may be required to minimize the
noise.
If the mobile phase used is not easily volatile, such as DMSO or DMF, temperature should be
increased to allow correct evaporation process.
The temperature can be readily adjusted during the method optimization process.
If you suspect that the compound of interest is thermally labile or semi-volatile, a lower
temperature could be used to improve the sensitivity by reducing the thermal decomposition
or evaporation. For a given flow rate and solvent, there is, however, a point at which the noise in
the chromatogram is dramatically increased because not all of the mobile phase is vaporized.
The minimum temperature that can be used is dependent on the flow rate and the nature of the
mobile phase.

Pretreat the Sample

If the sample contains any particulate matter, it should be filtered through a 0.2 or 0.45 µm filter
before injection.

Treat the Column

The chromatographic column typically contains microparticles which are used to separate the
compounds of interest. Under certain conditions, the column packing will undergo chemical
and/or mechanical breakdown, this may lead to the introduction of particulate matter into the
detector, which may lead to an increase in the noise.
The breakdown of the column packing is dependent on a variety of factors including the particle
size, type of column packing, the manufacturer of the column and the nature of the mobile phase
(high pH may degrade silica based columns).
When you install a new column, we suggest to pump the mobile phase through it for few minutes
before connecting it to the detector. This will flush out the microparticles that remained in the
column after its manufacture.

116 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Split Flow Rates and Ratios
The following table provides results of tests performed on a PLC 2250 Purification System
(250 mL/min max.) using the 1/16” OD, 0.005” ID, 35 cm PEEK splitter tubing.

Split Flow Rates and Ratios with Acetonitrile (ACN)

PLC with Integrated ELSD

Elution Flow Rate (mL/min) Split Flow Rate in ELSD (mL/min) Split Ratio (%)

5 0.08 1.62

10 0.29 2.87

20 0.70 3.51

50 1.58 3.15

75 2.04 2.72

100 2.36 2.36

150 3.14 2.09

250 4.77 1.91

Split Flow Rates and Ratios with 90% Water - 10% Acetonitrile (ACN)

Elution Flow Rate (mL/min) Split Flow Rate in ELSD (mL/min) Split Ratio (%)

5 0.12 2.32

10 0.24 2.44

20 0.47 2.33

50 0.80 1.60

75 1.01 1.34

100 1.21 1.21

150 1.61 1.07

250 2.35 0.94

PLC with Integrated ELSD | USER’S GUIDE 117

 Maintenance
The ELSD is designed to require a minimum of maintenance activities. If preventive maintenance
activities are followed, the detector should provide high sensitivity measurements without any
further intervention by the operator.

General Maintenance Recommendations

PLC with Integrated ELSD

● Maintain the ELS detector in a clean laboratory environment.

● If the ELS detector will not be used for one hour, flush out any mobile phase that contains
acids, bases, or salts to prevent foreign matter deposits on components or instrument
corrosion.
● Only use clean, particle- and oil residue-free gas.

Closing the gas supply while the pump is still running may result in serious nebulizer
damage.
NOTICE
If the ELS detector will not be used for one hour, close the shut-off valve on the splitter to
avoid clogging the nebulizer or depositing foreign matter into the detector.

After each run and before shutting down the PLC Purification System, the ELS detector should
be cleaned to ensure good performance. Follow the preventative maintenance steps below to
clean the ELS detector after the final analysis:

1. Allow the mobile phase to continue flowing after analysis to flush any remaining particles
from the detector. The mobile phase should not contain any additive or buffer.
2. Incrementally increase the temperature to dissolve any remaining deposits.
3. Stop the mobile phase flow, but allow the gas to flow for at least 30 minutes to prevent
particle deposit.
● If needed, stop the gas flow at the source.
● If needed, shut down the system.

The time required for each step depends on the application, so solvent type and sample
concentration should be determined accordingly. Access the inside of the instrument is not
required for routine operation. If the suggestions provided in this chapter do not remedy the
NOTE problem, contact your local Gilson representative. Refer to Customer Service on page 32.
The LED has a long but finite lifetime (5000 hours) and should be replaced periodically by
trained personnel. Decreasing light intensity from a failing LED will result in decreasing
signal heights. Contact your local Gilson representative to replace the LED.

118 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Troubleshooting
Considering that the operator has already determined that other components of the system are
operating in an appropriate way, the following information can help to determine the cause of
the problem.

Never disassemble the nebulizer. Disassembling the rear part of the nebulizer (at the level of

PLC with Integrated ELSD

NOTICE
the white engraved heat-shrinking tubing) will permanently damage it.

1. Check to ensure that the liquid level in the siphon is appropriate, and there is no liquid
pooling close to the evaporation tube inlet.
2. Check that the gas pressure is sufficient and stable. The selected pressure for most
applications is 2 bar (29 psi) and gas consumption is 3 L/min. Pressure above 4.5 bar
(67 psi) can damage the pressure sensor. The gas filter should be clean and in place. Only
use gas free of particle and oil residue.
3. Ensure that the flow rate of the pump is constant and check that there are no leaks in the
PLC system.
4. The mist from the nebulizer should be homogeneous. If it is not homogeneous, the nebulizer,
the needle or the PTFE tube may be partially obstructed. To remove the obstruction, pump
a solvent that can dissolve the foreign material. As an alternative, the nebulizer can be
placed in an ultrasonic bath to dissolve the foreign material. Instructions about cleaning of
the nebulizer are given on next page.
5. If the sensitivity of the detector is low, ensure that there are no leaks in the system. Make
sure you are using a fresh sample and consider running the test using a backpressure loop
instead of a column. Alternatively, the light source may need to be replaced or the nebulizer
could be obstructed.
6. A decrease in the sensitivity is often caused by the nebulizer (main cause). Clean the
nebulizer. If the sensitivity does not return to normal, the nebulizer might need to be
replaced. Please note that the root cause might also be in different module, i.e. volumes
injected by the autosampler might be too low or dead volumes in capillary connections
may cause peak broadening.
7. If the detector signal is saturated or if there is a decrease in the dynamic range of the
system, it is possible that a residue is passing through the detector cell. This will lead to an
intense signal due to a significant amount of light-scattering. This residue may be a result of
the elution of strongly retained materials from the column, or may come from the solvent.
To determine the cause of the problem, bypass the column and observe the signal intensity.
● If the signal returns to normal, strongly retained materials are eluting from the column.
Flush the column with a strong solvent to elute all material.
● If the signal does not return to normal, the solvent contains a too high residue material,
after evaporation and is not suitable for use with the detector.
8. If the noise of the detector without solvent is high or if ghost peaks occur, it is possible that
foreign material is present in the drift tube. In this situation, increase the temperature to 95°C
and pump appropriate solvent at the rate of 2 mL/min, using a gas pressure of 2.0 bar (29 psi).
The solvent will be determined by the nature of the samples that were previously analyzed with
the detector. If you do not know the nature of the sample, ethanol is a good choice. Do not
use solvents that can potentially corrode the instrument. Maintain the flow and temperature
during three hours at least.

PLC with Integrated ELSD | USER’S GUIDE 119

 Clean the Nebulizer
With time, the nebulizer can get clogged by sample and mobile phase materials. A dirty or
clogged nebulizer can cause increased baseline noise and decreased sensitivity. The following
procedure can be used to clean the nebulizer.
The nebulizer is a consumable component. Nebulizer lifespan depends directly on the conditions
PLC with Integrated ELSD

of use and care. This section provides general directions for nebulizer maintenance, but if cleaning
procedures are ineffective, consider a nebulizer replacement.

Handle the nebulizer carefully, and do not disassemble the rear component protected by a
white, heat-shrinking tubing. Improper handling of the nebulizer will permanently damage it.
NOTICE The nebulizer rear part results from a very tricky setting which must not be disassembled
for any reason. If it has been removed or unscrewed, the only solution is to proceed to a
nebulizer replacement.

Remove the Nebulizer

1. Switch off the PLC Purification System.
2. Disconnect the PLC Purification System from the mains.
3. Stop the flow of gas to the PLC Purification System.
4. Remove the chamber shield.
5. Disconnect the nebulizer liquid inlet from the splitter tubing.
6. Disconnect the gas inlet tubing from the nebulizer.
● To remove the tube, apply manual pressure to the white push sleeve to disengage the
grab ring, and then withdraw the tube from the fitting.
7. Hold the nebulizer with one hand, and then unscrew the black plastic nut to remove the
nebulizer from the glass chamber with the other hand. Do not pull or twist the nebulizer
capillary.
8. Remove the gas inlet fitting and the black plastic nut to avoid damaging the seals with the
cleaning solvent.

Clean the Nebulizer

1. Fill an ultrasonic bath with water. Fill a beaker (50 or 100 mL) with approximately 2 cm of
appropriate solvent. The solvent is dependent on the nature of the material that is present
in the nebulizer. In most cases, ethanol is a satisfactory solvent.
2. Place the nebulizer vertically in the beaker. The nebulizer outlet should be placed at the
bottom of the bath and the nebulizer inlet liquid tubing should be pointing up. Take care to
ensure that the rear component of the nebulizer is not in contact with the solvent.
3. Clean the nebulizer for approximately 30 minutes.
4. Replace the solvent with water and clean for an additional 30 minutes.

120 PLC with Integrated ELSD | PLC 2050/2250/2500 Purification Systems

Reinstall or Replace the Nebulizer
1. Reinstall the gas inlet fitting and the black plastic nut with seal.
2. Install the nebulizer in reverse order.
3. Install the chamber shield.

PLC with Integrated ELSD

4. Test the nebulizer to ensure that it is working properly.

Replace the nebulizer if the cleaning and preventative maintenance steps provided are
NOTICE
ineffective.

Decontaminate the Nebulizer

Set the evaporation temperature to 95°C and the gas pressure to 2 bar (29 psi). Pump the
appropriate solvent through the system at the rate of 2 mL/min. The solvent will be determined by
the nature of the samples that were previously analyzed with the detector. If you do not know the
nature of the sample, ethanol is a good choice. Do not use solvents that can potentially corrode
the instrument. Maintain the flow and temperature for a minimum of three hours.

PLC with Integrated ELSD | USER’S GUIDE 121

 Gilson Purification S.A.S.
LT380160-03 22, rue Bourseul - ZA du Poteau - F-56890 Saint-Avé, France
Original Instructions www.gilson.com/contactus