Real-Time PCR

LX150RTP
www.labdex.com | [email protected]
 Index

Sr.no Title Page no

1. Safety measure 2

2. Introduction 6

3. Features 6

4. Specifications 7

5. Installation 9

6. Operation 12

7. Maintenance 92

8. Replacement 93

9. Troubleshooting 94

1
Safety measures

Symbol Title Description

This symbol is used to inform you of
incorrect operations that could result in
physical injury or damage to the
Caution/Warning
instrument.
When the Caution or Warning sign appear,
please read the corresponding text, in
order to understand the type of
potential danger and any solutions that
have to be taken.
This symbol indicates prohibited
Prohibition behaviors. Continuation
of such behaviors could result in the
destruction of the instrument, or even
injury and death to the user.
Pinch Points Watch your hands at areas on the
instrument with this sign.
During the operation of the instrument,
the user might be exposed to or left over
Biological
with substances that are harmful or
Hazard
infectious to organisms. The operator
should acknowledge
its danger.
This sign is presented on areas on the
Hot Surface instrument that would emit high heat
during operation. Be warned of
burns.
Pinch Points Watch your hands at areas on the
instrument with this sign.

During the operation of the instrument,

Biological Hazard the user might be exposed to or left over
with substances that are harmful or
infectious. The operator should
acknowledge its danger.

2
 Protective Conductor This symbol is affixed next to a protective
Terminal conductor terminal on the instrument.

Carefully read this User Presented on the name plate of the

Manual instrument.

Presented on the name plate of the

CE logo instrument.
It indicates that the instrument fulfills the
requirements of relevant European
directives.
WEEE Presented on the name plate of the
(Waste from Electrical and instrument.
Electronic Equipment)
The instrument abides by the European
WEEE directives and is not allowed to be
disposed into the public waste disposal
system.
The power switch of the instrument is
POWER Power Switch near the position where the label is
pasted.

The standby button of the instrument is

Standby Standby Button near the position where the label is
pasted.

The USB port is near the position where

USB Port the label is pasted in the instrument.

The network cable port is near the

Network Cable Port position where the label is pasted in the
instrument.

It is pasted at the position of the fuse of

250V Fuse specifications the instrument, and the symbol marks the
T10AL
fuse specifications.

Indicates that the correct position of the

This side up transport package is upwards.

3
 The transport package contains fragile
Fragile, handle with care components, need to be handled with
care.

Keep dry The transport package need to be kept

dry, avoid rain.

Indicates that the packages are not to be

Stacking limit vertically stacked higher than 3 layers
(does not include the bottom package)
during static storage and transportation.
The transport package has to be kept and
Temperature limit handled within the temperature range of -
20 °C to 55 °C.

The transport package has to be kept and

Humidity limitation handled within the humidity range of 0%
to 85%.

1. To avoid electric shock, the input power cord for the instrument must be
properly grounded. The instrument uses a three-prong grounded plug with a
third (ground) pin that can only be used with a grounded power socket.
2. Operators are not allowed to disassemble the instrument. Replacing
components or adjusting certain parameters inside the machine must be
accomplished by the certified maintenance professionals
3. Before switching on the power, always check if the voltage of the power
supply matches the required power (100-240V～, 50/60Hz), and make
sure that the rating load of the power socket is not less than that of the
instrument 750W.
4. This instrument should normally be used with the supplied power cord.
If the power cord is damaged, it must bereplaced and cannot be repaired
5. When the power cord is inserted or pulled, be sure to hold the plug with
your hand in the correct position. While inserting, ensure the plug fully
and firmly inserted into the socket. While disconnecting the power cord,
do NOT pull the line but the plug out from the socket
6. The instrument must be installed in a place, where the power supply is
easy to be cut off.
7. The instrument should be placed in a clean, tidy workbench or table
with little dust and a place far away from the water source, such as pool,
pipe, etc. The room ought to be well ventilated, having no corrosive gas
or interference of a strong magnetic field. The table for the instrument
must be stable with the horizontal top
8. Do not place your hand nearby when the tray is in motion to avoid a
harm.

4
9. The instrument can be interconnected with computers, USB memory,
and USB scanners through the USB interface and network port

5
 Introduction

Real-Time PCR LX150RTP is a 96 well quantitative fluorescence Real-time

PCR System designed with 10.1-inch high-definition color touch screen and
builtin analysis software for spontaneous operation and analysis. High-
precision temperature control guarantees the block temperature resolution at
0.1 °C and temperature uniformity of 0.1°C. Gradient temperature control range
is 30-100°C and the maximum temperature difference is 40°C. It has two
temperature control modes settings which enables to control either block or
tube temperatures.

Features
1. It has 96-well high-throughput
2. Microprocessor control system with 10.1-inch large touch screen for
precise response and convenient operation
3. High sensitivity PMT (photomultiplier tube) detection
4. Sample tray automatically moves in/out, enabling integration with
automated workstations/plate handlers
5. Built-in software and PC software with flexible program settings
6. Preset templates + "Quick Run” starts experiment immediately

6
 Specifications

Model LX150RTP

Throughput 96 well (12 × 8)

Applicable consumables PCR single tube or strip, 96well*0.2ml
half skirted/no skirted plate
Dynamic range 1-10x10 copies
Detection location Top detection
Excitation light wavelength 400-800nm
Detection wavelength 500-800nm
Fluorescence channels Fluorescence channels
Fluorochrome/dy Channel 1: FAM/SYBR Green I Channel
2: JOE/HEX/TET/VIC Channel 3:
NED/TAMRA/Cy3 Channel 4:
ROX/Texas Red Channel 5: Cy5
Channel 6: Cy5.5
Excitation light source Full-spectrum LED
Detector PMT
Block temp. range 4~105℃
Block temp. accuracy ≤±0.1℃
Block temp. precision ≤±0.1℃
Max heating & cooling rate of block ≥6℃/s
Gradient temp. difference 1-40℃
Block temp. control mode Block mode, Tube mode
Sample volume range 5-100μL
Heated lid temp. range 30℃-110℃ (default 105℃)
Fluorescence intensity CV≤3%
Touch screen Yes, 10.1-inch touch screen
Scan mode Full plate scan or specified line scan
Software functions Absolute quantification, Relative
quantification, Melting curve, SNP
genotyping, HRM, Quick run, etc
Operating system PC software (Windows 8 or higher),
Built-in software
Ports USB Type-A port × 2, USB Type-B port,
RJ45 port
Power-off protection function Yes, data can be restored after power-
on
Sample tray control mode Sample tray automatically moves
in/out, enabling integration with
automated workstations/plate
handlers

7
 Dimension (W x L x H) 320 x 530 x 420 mm
Net weight 27 kgs
Gross weight 60 kg

Applications

Used for sequencing, genotyping, cloning and mutagenesis that require

quantification and detecting target nucleic acids, in institutes, research,
diagnostics centers and laboratories etc

8
Installation

A. Wire connection

Figure.1

i. As shown in the figure below, before using the instrument, connect the power
cord to the socket and turn ON power supply
ii. Power cord connection: The power cord attached to the instrument should be
used. When connecting, the power switch of the instrument should be turned
OFF after connecting, check the tightness of the power cord and the power
socket ofthe instrument. If it is loose, please replace it.
iii. Network cable connection: The network cable attached to the instrument
should be used. After connecting, check the tightness of the network
cable and the instrument network cable port. If it is loose, please replace
it.
iv. USB port: For external connection of USB devices
v. Code scanner: Before using the instrument, connect the code scanner with the
data line, and then insert it into the USB interface of the computer

9
 Figure.2

B. Preparation before startup

i. Check whether the working environment meets the requirements of the

working environment of the instrument before using it
ii. Place the instrument on a horizontal workbench
iii. The main view of the instrument is shown in the figure below

4
35

Figure.3

10
No. Description
LCD screen: fixed on the front top of the instrument. The user can touch
1
the screen for a series
of operations.
USB port: to connect to external USB driver, when connected, it can
2 performance the functions
like data import, export or software upgrade.
Standby button: When the instrument is powered on, press the button to
3
open/close the
instrument
Tray: It can be opened/closed by instrument terminal or PC terminal
software for placing/taking out samples.
4 The tray must not be blocked when it is opened and closed.
Do not block the tray with the hands when opening/closing, as this
may cause accidental injury.

11
 Operation

C. Instrument startup

i. Press the power switch on the back of the instrument to the "I" position to
connect to the power
ii. Press the standby button on the front of the instrument to turn on the
instrument
iii. The LCD screen of the instrument lights up and it can enter the login interface.
Entre the username and password to log in to the instrument terminal
iv. When using the instrument for the first time, the user needs to set the
initial password of the admin account through the instrument terminal in
the instrument and log in, then it can be used. When it is not used for the
first time, it can be used directly online through the PC, and it does not
need to login the instrument terminal.

Figure.4

D. Check before shutdown

i. Whether all test tubes and 96-well plates in the block of the instrument have
been removed
ii. Whether the inside of the hole in the instrument module has been cleaned
iii. Whether the tray is completely closed

12
E. Instrument shutdown

i. Press the standby button on the front of the instrument to turn OFF the
instrument
ii. Press the power switch on the back of the instrument to the " O " position
to disconnect the power supply
iii. If contaminants are spilled on the instrument, wipe it with 75% ethanol by a
soft cloth

❖ Software operation guide

After startup, the instrument enters the initialization and self-checking,

checking the functions of temperature control, optical detection, motor
activity, positioning, communication and so on. If there is any abnormality, an
alarm will be given. After passing the self-check, it will enter the user login
interface automatically

a. User login

• Initial login (Instrument only)

i. Before firstly using the instrument, the initial password of the administrator
account must be set through the instrument software (that is, the initial
password must be confirmed and login on the instrument terminal, so it can
be used online in the PC terminal for the first time, and then the subsequent
functions like creating a new user and deleting a user of the instrument can
only be performed on the PC terminal)

Figure.5

13
• Routine login (Instrument only)

If the instrument is not used for the first time, after it is powered on, enter the
user login interface, click the "User Login", and enter any user name and
password under the instrument for login

Figure.6

If you forget the password when logging in to the administrator account, click
the "Forget password" to generate an "Request code". The user can apply to
get an authorization code from the after-sales personnel to reset the
password of the administrator account

Figure.7

After login, the user enters the main interface of instrument terminal
software, which includes
o User Logout
o Template Library
o Import

14
o Quick run
o Real-time graphics
o Experiment library
o Settings

Figure.8

Instrument terminal cannot only run a experiment directly, but also import,
export and view file, or set the system.

• No need to login (PC connected)

If it is not used for the first time and the instrument has been connected
through PC there is no need to log in the instrument terminal and only the
viewing the experiment state function is effective. In the online mode, the
instrument terminal automatically jumps to the online interface of
experiment

15
 Figure.9

If the experiment is running in the online mode, click the button on the home
page to view the current experiment state

Figure.10

If the online mode is disconnected while the experiment is running, the

instrument status on the home page will change to: online disconnected,
running offline experiments

16
 Figure.11

Click the home button to enter the experiment status interface. The user
manually stops the offline experiment or when the offline experiment is
completed, the interface will jump to the user login (stand-alone mode

Figure.12

After reconnecting to the PC, the user can view the running experiments or
instrument experiment files to view the experiments that were running before
the disconnection

b. Run an experiment

Instrument terminal can run an experiment in the following two ways

o Via the template: Template → Click any template file → Plate settings →
Open module → Place samples → Close module → Next → Method
settings → Gain settings → Start run

17
 o Via the quick run: Quick run → Template selection → Open module →
Place samples → Close module → (Plate settings → Next → Method
settings →) Start run

• Plate settings

General setting of plate

After selecting the template, jump to the plate settings, which can set
the experiment name, target, sample, and select the plate

Figure.13

ii. Select the plate: the required hole in the plate can be directly selected
through the plate layout or well table on the right.
iii. Clear selection: the selected plate hole can be quickly cleared by clicking the
"Clear selection" button in the upper right corner
iv. Experiment Name: the creation time is the default name, or click to manually
edit the name

v. Fluorescent selection: select the fluorescence channel

• Sample: it can add or delete the sample information. After selecting the well

18
location of the plate, click the check box next to the sample ID to add the
sample to the plate

Figure.14

Click the "Add" button to add sample information. The sample ID can be added
by manual or by external code scanner

Figure.15

Select a certain sample information and click the “Delete” to delete this
sample information. If the sample information has been selected on the plate,
the system will pop up to prompt you before deleting

Figure.16

Switch between Plate and Table: you can switch the display of well plate
through plate or table

19
 Figure.17

• Plate information save as : After setting the plate, click the "Save As" to save
the information settings as a new template file

Figure.18

• Sample preparation

After setting the plate information, click the icon on the upper right of the
plate setting to open the module, and place the samples into the module
according to the configured plate layout. Click the icon again to close the
module.

• Experiment setup

Click the "Next" of plate setting interface to jump to the experiment setup
interface:

20
 Figure.19

Click the "Previous" to return to the plate setting interface to reconfirm. The
experiment setup interface can set the specific running program, select run
mode, set the hot lid temperature and liquid quantity, and also can save the
file as a template file

(1) Method View: Add add step, delete stage, delete step and set the
temperature & time of each program stage.
o Add Stage: click to select a holding stage, cycle stage, melting stage or
infinite stage.

Figure.20

o Add Step: click to add a step before or after the current step

21
 Figure.21

o Delete: it can delete selected steps or stages.

o Modify: it can set the target temperature, ramp rate, step hold time, and
whether to sample the fluorescence in the selected step.

Figure.22

More temperature parameter settings: the extended parameters of each

step can be set in the cycle stage

22
 Figure.23

Melting Parameters: the melting parameters can be set in the last stage of
melting

Figure.24

Settings of cycle number: click the cycle number box of the cycle stage to
manually edit the cycle number.

23
 i. Run Mode: Block or Tube temperature control mode can be selected
ii. Hot-lid Temp: if the function of hot lid temperature is enabled, the
heating temperature of hot lid can be set, ranging from 35 °C to 105 °C
iii. Liquid Quantity: set the liquid volume, and the default is 20 μl
iv. List View: switch to the list mode to view the running program.

Figure.25

v. Save As: click the "Save As" to save the setting information as a new
template file
• Start run
Click the "Start Run" to jump to the Run Confirm interface, where to select
the gain mode and setting the gain value

Figure.26

24
 After the gain setting is completed, click the "Run", the system will prompt
"please check whether the sample is loaded." Again

Figure.27

If no sample is placed, click the "Cancel" button to return to the Run Confirm
interface, click the icon on the interface to open the module, and place
the sample into the tray according to the plate layout. Then click the icon
again to close the module
If you confirm that the samples are placed into the block, click the "OK" to the
experiment begins and it will enter the real-time graphics interface

Figure.28

F. User logout

Click the “User logout” and confirm to exit the current user interface

25
 Figure.29

It cannot logout the current user when the experiment is running, a dialog box
will prompt to pause the experiment, or log out after the experiment is done

Figure.30

• Template library

i. It can review all template files (When using for the first time, the
administrator account has 4 types of experiments by default. Each type of
experiment can be set as an initial template, which can be saved as a new
template after setting the preset template, or import the template file with
the same user name on PC into the Instrument terminal), open, search,
select, export or delete a file
ii. Click any template file to open the template file and enter the plate setting
interface
iii. Template files under the instrument can be searched and viewed through
template name,time review, experiment type and user name (only the
administrator account can view all the files of all users in the instrument,
while ordinary user can only view the files under his own name)

26
 Figure.31

Click Import, it can import the template file from the same user name
through the external USB drive

Figure.32

Check the template file to export or delete the selected file

27
 Figure.33

• Import

It can import the template files under the same user name into the
Instrument terminal software through the external USB driver

Figure.34

• Quick run

Click the "Quick Run" to select the preset template (the template file settings
and gain settings

28
 Figure.35

If there is no need to change experiment settings after selecting the

template, click the icon on the upper right to open the module and place
the samples into the block according to the plate layout. Then click the icon
again to close the module and click the "Start Run" button to run the
experiment

G. Real time graphics

Click Real-Time Graphics. If the program is not running, click the "Real-Time
Graphics" and a pop-up prompt will appear

Figure.36

29
 Figure.37

1) Experiment running time: the start time of experiment can be viewed at

the top of the interface, and the complete time and total running time can
also be viewed after completion
2) Run status: displaying the running status of experiment
3) View experiment data: slide the interface to switch to the viewing
interface of different experiment result curves and the viewing interface
of running programs
4) View run parameter: the experiment running parameters of target stage
(step) can be checked
5) Experiment operations: the "Skip Current Stage" and "Stop Run" can be
carried out, and the "Cycle +1" or "Cycle -1" can be operated after entering
the cycle stage
6) View the error log:

If there is an error warning during the running, the interface will pop up
a warning box, and the user can decide to stop or continue running
according to the warning content:

Figure.38

After the running continues, the user can click the "View" next to the error
log to check all error logs

30
 Figure.39

Figure.40

H. Experiment library

i. Review all experiment files under the user name, and search, view, select
or export the file.
ii. The experiment files in this instrument can be searched and viewed
by the experiment name, finish time, experiment status, experiment
type and user name (only the administrator account can view all the
files with different user names in the instrument, common user can
only view the files under their own user name):

Figure.41

31
 Figure.42

Or click the "View Run Parameter" button at below to switch to view the
experimental running parameters

Figure.43

The experiment files that are selected in the checkbox can be exported as
CSV files or directly as .epf format experiment files. The user can also click
"Export Encrypted CSV" to encrypt and protect the files that need to be
exported, or delete the selected experiment files

32
 Figure.44

The password needs to be set by yourself. After setting the password, click
"Export"

Figure.45

I. Settings

• Basic settings

Set screen display brightness, instrument date, lock screen, completion

prompt sound, alarm sound, administrator account can also upgrade
software through U disk

33
 Figure.46

After selecting "Open" at the lock screen function, you can set the lock screen
time

Figure.47

After the instrument locks the screen, you need to enter the login password
and unlock:

34
 Figure.48

If the user did not click “Save” after modifying the parameters, it will exit the
setting interface directly, but the systemwill pop up a prompt box to ask the user
whether save the modified parameters or not

Figure.49
J. Network settings
By performing network settings, the user can view the IP address of the
Ethernet connection

35
 Figure.50
K. Quick run settings

It can set template files and gain setting parameters for quick run

Figure.51
There has up to 5 preset quick run templates, and one of them can be set as
the default template. For each template, it can be selected as the template file
to run one experiment

36
 Figure.52

• Error log review

The administrator account can view the error log of the instrument
(Common users do not have this function), including the error code, time of
occur time and user name

Figure.53
Common user system setting interface (no option of error log review

37
 Figure.54

• About
About includes instrument software version and it’s release version, the
instrument model and serial number

Fiugre.55

38
 PC software installation and login

L. Software installation

1) Hardware requirements

- A computer with at least the following configuration

- Intel i3 CPU
- 2GB RAM
- 200GB available hard drive space
- At least one USB-A port
- At least one Ethernet port

2) Software requirements

- Windows 8/8.1/10 operating system and compatible versions

- SQLite 3 database software and compatible versions
- Net Framework 4.6.1 and compatible versions

3) Installation method
Double-click the setup file → select the target folder → select the start
menu folder → select additional tasks → prepare the installation
confirmation interface → installing → complete

39
Figure.56

40
 Figure.57

• User login

Double-click the icon on the desktop to start the software. The software
automatically initializes the configuration and detects the software operating
environment. After the startup is completed, it enters the user login interface
of the Real-Time Fluorescent Quantitative PCR System

Figure.58

i. Initial login: Login: Enter the instrument serial number, administrator

account, and the administrator account password set for the first time on
the instrument terminal to log in

41
 Figure.59

a) When the PC software is used for the first time, if it is not detected
that the instrument is connected, a pop-up window will prompt
"Instrument not connected!".

b) When the instrument is connected, the PC software reads the

administrator password on the instrument. If there is no
administrator password, a pop-up window prompts "Please set
administrator password on the tablet before login

2) Regular login

After the initial login, multiple user names can be set on the PC side
(up to 20, see 7.4.1 introduction for specific operations), and
subsequent logins can be logged in with the administrator or common
user name and password

42
 Figure.60

After logging in, enter the main interface of the Real-Time Fluorescent
Quantitative PCR System, the main interface include:

o The top menu bar contains 8 main function buttons, including File, User,
Instrument, Analysis, Tool, Report, Database and Help
o The function keys below the menu contain 5 buttons: New, Open, Save,
Close and Open Module;
o The shortcut keys on the main interface contains 4 buttons: Open template,
Quick run, New experiment and Open experiment
o The status bar at the bottom displays the connection status, instrument
serial No., and currently logged-in user name, and the module status is also
displayed in the online status
o The main interface and experiment interface can be switched in the form
of option bars in the interfaceoption bar switching area

Figure.61

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 • Experiment interface operation

It can enter the running experiment interface in several ways

The navigation bar of the experiment interface after the initial creation of a
new experiment contains two option bars for Setup and Run

Figure.62

After the experiment is running, the navigation bar of the experiment

interface adds two option bars of Analysis and Report

Figure.63

M. Experiment information settings

• Properties settings

The "Properties" must include "Experiment Name", and "Experiment Name"

defaults to the current time, with a maximum length of 20 characters, and
cannot start or end with a space; "Comment" and "User Name" are optional fill
in the targets, choose to fill in as needed

44
 Figure.64

Experiment type: Absolute, Relative, SNP or HRM can be selected according

to the type of experiment

Figure.65

Run mode: Block or Tube can be selected according to experimental needs

Figure.66

Block scan method: According to the sample situation in the experiment, it can
choose scan all lines or scan specified lines. For scan specified lines, it can
select any required line or multiple lines in A-H to scan (select at least one
line to scan

Figure.67

• Target settings

Figure.68

i. Click "Add " to add targets one by one (the default is target 1, and the
maximum is 100).
ii. For each target, click the "Target Name" to edit, click the drop-down

45
 "Reporter" to select the dye used in thisexperiment (the default is FAM, there
has total 13 fluorescent dyes for option), click "Color “to choose different
colors (up to 48 colors for option), double-click the "comment" to edit the
contents; the Endogenous Control check box can be selected according to
the needs of the experiment (only for the relative quantitative PCR
experiment use)

◼ Channel 1：FAM/SYBR Green I;

◼ Channel 2：JOE/HEX/TET/VIC;
◼ Channel 3：NED/TAMRA/Cy3;
◼ Channel 4：ROX/Texas Red;
◼ Channel 5：Cy5;
◼ Channel 6：Cy5.5.

Figure.69

iii. Click "Save to Library" to add the selected target (the selected target bar
turns pink) to the detection target library. The name of the added detection
target cannot be the same as the name of the existing detection target in
the library
iv. Click "Import from Library" to directly import the existing targets in the
inspection target library. The imported inspection target name cannot be
the same as the added inspection target name
v. Click "Delete" to delete the selected target in the list
vi. Click "Clear All" to delete all targets added this time
vii. Click the drop-down box of "Passive Reference Dye" to select the reference
fluorescence of this experiment
viii. Click "Gain Settings" to pop up the interface, it can choose auto gain (500-
60000 can be set) or custom gain (1-20 can be set), and then choose
whether to use the default value, if not use the default value, manually enter
the parameters of each fluorescence channel

46
 Figure.70

• Sample information settings

Figure.71

i. Click "Add " to add sample information one by one (add up to 96).
ii. For each sample, the corresponding information can be selected in the
"Sample Columns" in the toolbar. The color and sample No. are mandatory,
and the rest can be edited and filled here when selected

Figure.72

iii. The input of hospital sample ID and other information can also be
scanned and input by connecting a code scanner through the USB
interface
iv. Click "Batch add " to pop up the box for adding samples in batches, enter

47
 the number of samples that need to be added, and click "Add" to quickly
add the corresponding number of samples

Figure.73

v. Click "Import " to import the sample information file in .xlsx format
vi. Click "Export " to export the sample information file in .xlsx format.
vii. Click "Delete" to delete the selected sample information.
viii. Click "Clear All" to clear all sample information added this time

• Plate settings

Figure.74

i. Click "Plate" or "Table", select the plate wells that needs to be set, and set
the target and sample information of these wells on the left
ii. Target setting: select 6 different concentration units according to the
drop-down box (or not selected); check the concentration box, 4 different
attributes can be selected from the attribute drop-down box, after setting
the corresponding plate, the attribute identifier and target name will
appear on the screen (the table will record the set target information); up
to 6 different targets can be set for the same well
iii. Sample information setting: it can be marked by the check box on the
right side of the sample name. After checking, the corresponding sample
name will appear in the plate, and the background color will change to the

48
 color corresponding to the sample (The table will record the set sample
information).
iv. After setting the plate information, select the three drop-down boxes of
"Sample", "Target", and "Property" after "Select Wells" at the top right to
view the corresponding wells
v. In the plate interface: click the magnifying glass symbol on the upper
right to enlarge the plate layout; click the shrink symbol to reduce the
plate layout; click the restore symbol to restore the layout to its original
state
vi. In the plate interface: by right-clicking, it can clear the settings of the wells
(clear the target settings, clear the sample settings or clear all settings),
copy and paste operations

• Method settings

Figure.75

i. Hot-lid Temp: check the checkbox to set the heating temperature of the
hot lid, the setting range is 35°C-105°C
ii. Liquid Quantity: set the sample dosing volume
iii. Add Stage: The user can create a new Holding stage, cycle stage, melting
stage or Infinite stage through the drop-down box (directly click the Add
Stage to create a new cycle stage by default)
iv. Add Step: the user can add a step before or after the selected step
through the drop-down box, (directly click “Add step” to add a new step
after the selected step by default, each stage can be set up to 20 steps)
v. Delete: delete the chosen stage or step
vi. Parameter setting of holding stage, cycle stage, melting stage or infinite
stage. In each stage, the user can set the ramp rate, target temp., step
hold time, and sampling fluorescence or not. In addition, the cycle stage
can additionally set the extension parameters of each step (extension
temp., extension time, grad temp., and extension start cycle). the melting
stage can additionally set the melting parameters of the last step of the
melting step (melting step temp., melting step hold time)

49
 Figure.76

• Sample preparation

After setting programs and before running, please make sure that the
reaction samples are ready
- Make sure to use appropriate consumables
- Make sure that the arrangement of the PCR reaction plate is consistent with
settings of the above plate. When ready, click "Open Module" button in the
function keys below the menu bar, then place the samples according to the
plate layout, insert into the module tray, and then click "Close Module

• Start to run

i. Start to run: After the preparation, make sure that the PC is connected and
the instrument is idle without abnormal alarms, then click Run. On the
right side of the "Run Status" box, click the "Start Run" button to start
running the experiment. The experiment in running is marked with a green
circle

Figure.77

ii. Offline operation: If the connection with the instrument is

disconnected during the experiment, the instrument will continue to
run the experiment offline. After the operation is completed, reconnect
the instrument and the PC software, and the user can go to the menu bar→
view instrument-side experiment files, jump to the instrument-side
experiment file library and view the list of experiment files.

50
 iii. Running status: After the experiment starts, the “Run Status” box will
display the experiment starting time, the experiment complete time, the
“Run Status” (including 5 states of not run, running, failure, stop by user
and run completed), Remaining time, Currently running segment, Cycle,
Step, Hot lid temperature, Target temperature, Actual temperature,
Gradient temperature

Figure.78

iv. After running, the user can perform "Skip current stage" or "Stop". During
the running of cycle stage, the user can operate "Cycle +1" to add one cycle
or "Cycle -1" to delete one cycle
v. Fluorescence curve: the fluorescence intensity will change with cycles. The
curve will appear after at least one cycle finished. The well can be selected
on the right side. On the left side, the user can set the targets for view and
the curve color of the selected well (all displayed by default). the mouse is
placed between curve frame and the hole position frame, the user can
adjust the display range. the mouse is placed on the curve interface, and the
curve coordinates can be zoomed by scrolling the mouse. click on the curve
to perform settings of the graph as "Save image", "Copy to clipboard", "Print"
and " Fix size
vi. Temperature curve: the temperature changes with the experimental
time, and it appears after the running. scroll the mouse to zoom in and
out of the curve coordinates. click on the curve to perform settings of
the graph, as "Save image", "Copy to clipboard", "Print" and " Fix size".
vii. Melting curve (it has no melting curve if no melting stage is set in the
running program): the fluorescence intensity changes with the
temperature and appears after completing a melting. scroll the mouse
to zoom in and out of the curve coordinates. click on the curve to
perform settings of the graph, as "Save image", "Copy to clipboard",
"Print" and " Fix size".
viii. Method: user can view the specific program of this running experiment, but
can't modify it.

N. Analysis

After the running is completed, the analysis bar will automatically appear
in the experiment interface, including display option buttons for different
result curves, such as amplification curve, standard curve, melting curve, etc

• Experiment result curve

The analysis functions for different experimental results are as follows

Amplification curve and The amplification curve and amplification analysis

amplification analysis result of the reaction well can be viewed.
result

51
viewing function

Standard curve and Check the standard curve, specific parameters and
standard analysis result analysis results according to the test target. The user
viewing function can also view the imported standard curve
information, and the user can also export the
standard curve or save the standard curve to the
standard curve
library.
Melting curve and The user can view the melting curve and melting
result of each test
melting analysis result
target in each reaction well, as well as the
viewing function fluorescence curve and derivative curve.
Relative quantitative The user can view the relative quantitative graph and
graph and relative the relative quantitative analysis results.
quantitative analysis
result viewing function
SNP chart and SNP The user can choose to view the SNP map and SNP
analysis result analysis results
viewing function according to the test targets.
HRM curve and HRM The user can view fluorescence curve, derivative
analysisresult viewing curve, normalization curve and difference graph.
function

• Analysis

"Analyze" and "Analysis Setting" buttons will appear at the upper right of
each curve result interface. Click the "Analyze" button to analyze the
experimental results (according to the default parameter settings).

Figure.79

• Analysis setting

i. If the user needs to adjust the analysis parameters and re-analyze, click
the "Analysis Setting" button to set different analysis parameters, and
then click "Apply Analysis Settings" to analyze according to the newly set
parameters
ii. For the amplification curve, there has Ct analysis parameter settings and
the advanced parameter settings
- Ct analysis parameter settings: Select the stage to use for Ct

52
analysis→Select the algorithm to calculate Ct→Select whether to
use s-shaped curve fitting→Set thresholds for each target, Start
cycle and End cycle

Figure.80

The advanced parameter settings: Auto Baseline, Start Cycle and End
Cycle are set for each target at each well

Figure.81

53
 The standard curve parameters can be set in the standard curve
settings: The standard curve acquisition method is set, and it
will use the standard curve from this experiment by default (the
standard curve is available only when the test target contains at
least 2 standard products with different concentrations;
otherwise, the target has no standard curve); If the standard curve
imported from external is selected, targets, imported standard
curve file and standard curve used need to be set
- External import standard curve setting: Click the target in an experiment,
click "External Import", the selection box of standard curve file will pop up
(only the standard curve stored by the current user willbe displayed, and all
standard curves will be displayed when the administrator logs in)→Select
the filethat meets the standard file format and click "OK" → drop down to
select the detection target in the imported standard curve
- If the generated sampling number of the imported standard curve is
inconsistent with the current sampling number, the statement " Sampling
count of imported standard curve is not match to current experiment” The
prompt
- If the generated sampling temperature of the standard curve is
inconsistent with the current sampling temperature, the statement "
Import failed: sampling Temp. not match. ” The prompt
- If the content of the imported standard curve file cannot be analyzed, the
statement " Cannot find valid standard curves.” The prompt

Figure.82

It can set the melting curve parameters in the melting curve

settings: Select the Melting step to be analyzed→Select whether

54
 to enable advanced fitting and set the Min. Tm, Max. Tm Width, and
Min. Tm Width for each target

Figure.83

vi. It can set the parameters of relative quantitative analysis in the relative
quantitative analysis settings: Choose the Analysis Method of
Relative Quantification→Select Reference Sample（ One, which can
be selected from the Settings sample, or null, which is null by default
）→Set Endogenous Control (all targets can be selected, not selected
by default ） →Select the relative quantitative confidence interval
algorithm and set the algorithm parameters (The Confidence Level is
given as a percentage. Optional 95.0%, 97.0%, 99.0%, 99.5%, 99.7%,
99.9%; Multiple of Standard Deviations can be 1, 2 or 3）

55
 Figure.84

In the SNP settings, it can set the parameters of SNP analysis: Drop
- down to select SNP analysisdata→Check whether each target keep
manual calls

Figure.85

In HRM settings, it can perform the settings of HRM curves: Set Pre-

56
 Melting Start Temp., Pre-Melting End Temp., Post-Melting Start Temp. and
Post-Melting End Temp. for each target

Figure.86

O. Report

After the running is completed, the report column will automatically appear in
the experiment interface, including the Print and QC Summary tabs

• Print

i. Only when the experiment is absolute quantification or SNP, and the status
of experiment file is completed, the report can be viewed and printed
ii. The interface on the right side of the printed report displays the judgment
result corresponding to each sample and its target. When viewing the report
for printing, the analysis results of each target in each well are arranged by
default according to the well number

Figure.87

iii. Report Template: drop down the selection box to select the report template
to be printed, and the report will be printed in the form of a print report
template

57
 Figure.89

iv. Print Settings: Click this button to pop up the print settings dialog box,
enter the content required in the report template

Figure.90

v. Negative Judgment Settings: Click this button, the negative judgment

setting dialog box will pop up, and the user can set the negative judgment
parameters

58
 Figure.91

vi. All: select all information targets in the list for printing, and the tick box in
front of all information targets are automatically selected
vii. Cancel Selection: Click this button, all the selected information targets in
the list will be canceled
viii. Information targets tick: the user can print each target of each sample.
All can be ticked or canceledautomatically, or manually ticked or
canceled individually
ix. Print Preview: Click to pop up the print preview dialog box for reviewing
x. Print Settings: Click to pop up the Windows print setting interface. After
setting parameters, such as the number of copies, click to print the report
in the form of the report template

• QC summary

In the quality control summary interface, the user can view the amplification
curve, the user can select the well to viewthe corresponding curve of the
well, and the user can also select the Curve Color, Gragh Type, and
displayed fluorescence channel of the Amplification Curve. The user can
view the results of QC information (displaying the unknown samples and
standards of the specific non-amplified).

59
 Figure.92

i. Curve Color: drop-down box to select and set the curve color
ii. Graph Type: drop-down box to set the amplification curve style.
iii. QC Summary bar: If the QC information is ticked to be used here, it will
display the wells that meet to the rules in the result bar on the below
iv. QC summary result bar: it displays the wells that meet to the selected QC
information rules, and the format is displayed as "test target 1: well 1, well
2, .... test target 2: well 1, well 2,.
v. Display the amplification curve: After selecting the well, the
corresponding curves will be displayed
vi. QC information results: display the unknown samples and standards of
the specific non-amplified.

❖ Menu bar function description: File

P. New

o New experiment

File→New→New Experiment, create a new experiment (default named as

current time), and then turn to the experiment interface. For specific
operations on the experiment interface:

To create a new experiment, the user can also

- Click "New" directly in the function keys below the menu

- In the main interface, directly click the shortcut key of "New Experiment

60
 Figure.93

• New experiment from complete

File→ New→ New from template, the "Select Template" box will pop up

Figure.94

Select and open any template file in the Template Library, the system will
automatically generate a new experiment file (default named as current time),
and then turn to an experiment interface which saves the setting data in the
template, and the user can directly run the experiment according to the
settings in the template

To create a new experiment from a template, the user can also

Click the drop-down box of "New " in the function keys at the bottom of the
menu

Figure.95

61
• Open (Experiment file)

File → Open → Turn to the "Select Experiment File" box

Figure.96

This interface displays the list of all experiment files, the user can sort,
search, open and close the experiment files. the specific operations are as
follows
1) Sort: click the head of the list to sort the experiment files
2) Search

i. Search drop-down box: click the drop-down box of the Search Option, and
the user can select the experiment name, Experiment File Template
Creation Time, Recent Open Time, Experiment Status, Experiment Type,
User Name (only the administrator account can view all files of all users
in this instrument, ordinary users can only view the files of their own user
name) to search for experimental files. When searching, up to 4 search
targets, and the selected search targets cannot be selected again

Figure.97

62
 Figure.98

ii.Search: click this button to search the experimental file library with the set
search conditions
iii. Clear. click this button to clear all the contents in the search options
3) Open: Select an experiment in the experiment file list, and then click this
button to open the selected experiment
4) Close: click to close the " Select Experiment File " interface.
To open the experiment file bars, the user can also

Directly click "Open" in the function keys below the menu

Directly click the shortcut key "Open experiment" on the main interface:

• Open template ( File)

File → Open from template → Turn to the "Select Template" box:
This interface displays the list of all template files, and the user can sort,
search, open and close with the specific operations as follows:
(1) Sort: Click on the head of the list to sort the template files.

(2) Search:

i. Search drop-down box: Click the drop-down box of the search option,
and the user can search for template files by the Template
Name,Template Creation Time, Recent Open Time, User Name (only the
administrator account can view all files of all users in this instrument,
ordinary users can only view the files of their own user name).

63
 Figure.99

i. Search: click to search the template file library with the set search
conditions.
ii. Clear. click to clear all the contents in the search options

(3) Open: select an experiment in the template file list, and then click this
button to open the selected template file, and automatically create a new
experiment every time the user can open one file (default named as
current time), and turn to the experiment interface. For specific operations
of experiment interface
(4) Close: click to close the "Select Template" interface

(5) Click the drop-down box of "Open" in the function keys below the menu

(6) Directly click the shortcut key "Open template" on the main interface:

Figure.100

• Import expe3riment file

Import the template file from outside:

File→Import template file→Import file selection box pops up (file opening
dialog box of Windows system )→Select the file in the format of .ept→Open

64
 Figure.101

• Export Excel

Export Excel data:

File → Export to Excel→ Select hole arrangement → Select Export path →

Select Export content → Click OK. If you want to encrypt the exported Excel,
you can check "Export Encrypted" and set a password to encrypt the
exported Excel;

Figure.102

• Save

Save the modified information of the experiment interface to a file

If the user opens an experiment file and modify it, click “Save” to save the
modified information to the original experiment file

65
 If the user opens a template file and modify it, click “Save” to save this file as
an experiment file, and a prompt box will pop up, and the user need to enter
a new experiment file name

Figure.103

To save file information the user can also:

Directly click “Save” in the function key below the menu

• Save as (Experiment file)

Save the file as an experiment file.

In the experiment interface: File → Save As → Enter the experiment file name
(default named as creating time) → save as an experiment file

To save as experiment file user can also

Click the drop-down box of "Save" in the function key area below the menu

Figure.104

• Save as a template ( File)

Save the file as an template file

In the experiment interface: File→Save As Template→Enter the template file

name (default named the file creation time)→Save as template file

To save as template the user can also

Click the drop-down box of "Save" in the function key area below the menu

66
 Figure.105

• Export standard curve

Save experimental information as a standard curve file: Only the opened

experimental file has been run with the standards set, it can it be operated

File→Export standard curve→Enter the file name of standard curve→Save

• Exit

Exit the Real-Time Fluorescent Quantitative PCR System directly: File→Exi

If there is still an experimental interface that has not been closed, it will
prompt

Figure.106

Q. Meni bar function description: User

• User management

When the instrument is connected, User Management can be performed. It

will display the list of user informationincluding No., User Name, User Status,
Created Time, Last Login Time of the user, and current user status

67
 Figure 107

Common users can only view their own information and modify their own
passwords; administrators can view all user information under the instrument
but can only modify its own passwords. At the same time, the administrator
account also has the authority to create a new user, edit users and delete
users

(1) Modify Password: change the password of your own account

(2) New: to create a new user information, it needs to set a user name and
password

Figure.108

(3) Edit: to edit a user, it can reset the existing user password and unlock or
lock the user status. The reset user password is unified as: 888888

68
 Figure.108

(4) Delete: deleting user information will delete the user name and all templates
under this user name in this machine

Figure.109

- User name format requirements: numbers, uppercase and lowercase

letters, spaces, underscores, minus signs, Chinese characters, but cannot
start or end with a space. A string of 1 to 15 characters (one Chinese character
counts as one character). Used user name cannot be used again (for the
same instrument batch number).
- Password format requirements: numbers, uppercase and lowercase letters,
the length is 6 to 12 characters.
- If there are 20 users whose status is not "deleted", the "New" button is not
selectable.
- To create, edit and delete users, it needs to enter the administrator to get
administrator authentication. If the administrator password is not
entered, a pop-up window will prompt "Please enter the administrator
password!"; If the administrator password is entered incorrectly, it
prompts " Wrong Admin Password!" automatically; If the password is
entered incorrectly for 5 consecutive times, it will automatically end the

69
 current operation (close the relevant pop-up box) and prompt "
Administrator authentication failed, operation failed!"

• User login

For user login and switch user to login

User→ User Login→ Enter the login interface: select the instrument serial
number, user name, and password→ click Login→ Enter the new user
interface

Figure.110

R. Menu bar function description: Instrument

Instrument includes connecting the instrument, disconnecting the

instrument, viewing the instrument information, setting the instrument alarm
sound, viewing, running and importing the experiment, including the firmware
upgrade

• Connect

If the instrument is online, it will automatically connect to the instrument

when logging in; if the instrument is not online when logging in, and the
instrument is set to automatic connection when online, it will automatically
search to check whether the instrument is online and will connect to the
instrument after it is online. Otherwise when selecting the instrument
connection, it will try to connect to the current instrument

It can set the automatic connection or manual connection, and the priority of
connection between the USB port or network port

Instrument → Connect→ select port or auto-match port

Figure.111

After the connection is successful, the status bar becomes: Connected. At this

70
 time, according to the controllable state of the instrument, it can be divided
into two online states

- Connected, Not Controlling”: When the instrument terminal is logged in

online and the instrument terminal rejects PC control, data can only be
transmitted between the PC and the instrument terminal, but not the
operation of the instrument.
- Connected, Controlling”: When the instrument terminal is not logged in or the
instrument terminal is controlledby the PC, the PC can control the instrument
in this state, and the instrument terminal can only view the experimental
status

• Disconnect

Instrument → Disconnect → Disconnect the currently connected instrument

If the instrument is running the experiment, it cannot be disconnected. Click

"Disconnect" and the following prompt will pop up

Figure.112

After disconnection, the status bar becomes: No Connected.

• Instrument information

When the instrument is connected, you can view the information of the
currently online instrument, including basic instrument information and
instrument abnormal information:

Basic instrument information: instrument batch number and current

instrument status

Instrument abnormal information: recent abnormal operation log, such as:

Abnormal No., Abnormal Specific Contents, and The Time When The
Abnormity Happens

71
 Figure.113

• Alarm

View and change the settings of the current instrument alarm sound

Figure.114

• Show running experiment

When the instrument is running an experiment, click this button to quickly

jump to the running experiment interface.

• View instrument side experiment files

Jump to the instrument-side experiment file library to view the list of

experiment files:

72
 Figure.115

The user can search by experiment name, experiment status, experiment type and
user name. After searching, select the required file, and then import the selected
experiment file from the instrument to the PC

Figure.116

• Quick run

In the controllable state of the online instrument, and the template file has
been stored in the template library (the plate information has been set), you
can quickly create an experiment and start the run through the “Quick run”
button.

The "Quick Run" under the instrument in the top menu has the same function
as the shortcut key "Quick Run" on the main interface. Click to jump to the
"Quick Run" interface, where you can edit the basic information of the
experiment and click to import the template

73
 Figure.117

Select the file in the template library and open it:

Figure.118

After confirming, click to start running

74
 Figure.119

Confirmation of running parameters

Figure.120

After confirming, click "Run" to enter the experiment running interface

75
 Figure.121

S. Calibration

• Calibration instrument

The user can view the calibration status data, gain values and other
information of the online instruments.

Figure.122

• Baseline calibration

Instrument→Calibration→Baseline Calibration→Refer to the contents in

"7.2.1" to set relevant information as required and run the experiment. After the
operation is completed, the baseline calibration data of the online instrument

76
 will be output
- Only administrator can have this function.
- This function can be selected only when the online instrument is in idle state,
and the calibration experiment cannot be resumed if power failure and
recovered.
- If the calibration procedure is not completed normally, the current calibration
will be invalid and the calibration data will not be saved

• Fluorescence increment calibration

Instrument-Calibration-Fluorescence Increment Calibration- to set relevant

information and run the experiment as required. After the operation is
completed, the fluorescence incremental calibration data of the online
instrument will be output.

- Only administrator can have this function

- This function can be selected only when the online instrument is in idle state,
and the calibration experiment cannot be resumed if power failure and
recovered
- If the calibration procedure is not completed normally, the current calibration
will be invalid and the calibration data will not be saved

• Crosstalk calibration

Instrument-Calibration-Crosstalk Calibration-Refer to the "7.2.1 " to set

relevant information and run the experiment as required. After the operation
is completed, the crosstalk calibration data of the online instrument will be
output

- Only administrator can have this function

- This function can be selected only when the online instrument is in idle
state, and the calibration experiment cannot be resumed if power failure
and recovered
- If the calibration procedure is not completed normally, the current
calibration will be invalid and the calibration data will not be saved

• Crosstalk gain calibration

Instrument-Calibration-Crosstalk Gain Calibration-Refer to the step "7.2.1 " to

set relevant information and run the experiment as required. After the
operation is completed, the crosstalk gain calibration data of the online
instrument will be output

- Only administrator can have this function

- This function can be selected only when the online instrument is in idle
state, and the calibration experiment cannot be resumed if power failure
and recovered
- If the calibration procedure is not completed normally, the current
calibration will be invalid and the calibration data will not be saved

77
T. Menu bar function description: Analysis

• Analysis

After the experiment is completed, click to the analysis or report column of

the experiment interface, the analysis function key in the menu bar is
available, and the function is the same as the analysis button of the
experiment interface

• Analysis settings

After the experiment is completed, click to the analysis or report column of

the experiment interface, the analysis setting function key of the menu bar is
available, and the function is the same as the analysis setting button of the
experiment interface

U. Menu bar function description: Tool

• Network configuration checking and setting

The user can view the local IP of the instrument, add the IP of the instrument,
and communicate when the two are on the same network end

Figure.123

• Automatic lock settings

After automatic lock is enabled, you can set the automatic lock waiting time

78
 Figure.124

If you do not perform any operation within the specified time, the PC will
be automatically locked and you need to re-enter the current user
password to unlock the PC

Figure.125

• Scan method

Select the module scan method of “Scan All Lines” or “Scan Specified
Lines” (it can select any required line or multiple lines in A-H to scan, by
default all lines A-H are selected and scanned)

79
 Figure.126

• Run mode

Block mode or Tube mode can be set for temperature control

• Sample columns

It can tick the required sample columns, among which "Sample No." and
"Color" are mandatory by default

Figure.127

• Run confirm items

Select to confirm again the key information before running the experiment
file. Before running, several ticked targets will be judged. If the information is
not complete, the experiment cannot be started

80
 Figure.128

• Target library

Create, edit, delete and clear the targets files of four kinds of experiments:
"Absolute ", "Relative ", "SNP" and "HRM”

Figure.129

(1) Sort: Click the header of the list to sort the corresponding tests

Figure.130

(2) Search: search by Creation Time, Target, Reporter and Modify Time

81
 Figure.131

3) New: create a new target

Figure.132
(4) Edit: Edit the selected target

Figure.133
(5) Delete: delete the selected target (multiple targets can be deleted at the
same time)

Figure.134
(6) Clear: Delete all target

82
 Figure.135

V. Menu bar functions descriptions: Report

• Select default template

The default print report for absolute and SNP type experiments can be
selected, and the report format can be previewed

• Print template

It can set the manual editing items for printing report template of the absolute
and SNP type experiment, and select the printing paper and printer

Figure.136

83
• Default negative judgment

Set the default negative judgment parameters

Figure.137
• Report template library
Management of the report template library. It displays the list all report
templates, and can create a new template, edit the existing template, import
a template, export the template and delete templates

Figure.138

(1) New: for absolute quantification and SNP only, it can create a new absolute

84
 or SNP report from the drop-down box

Designer of absolute report template

Figure.139

Designer of SNP report template

85
 Figure.140

(1) Edit: to edit the selected report template, the interface is the same as
“New”.
(2) Import : import report template file from outside, the supported file format
is .rpt.exp
(3) Export: export the selected report template, and the exported file format is
.rpt.exp
(4) Delete: delete the selected report template.

• Consolidated report

Open the experiment file that has been run and click "Consolidated Report"
to view and print the consolidated report ofthe experimental results (the
report will take some time to generate, please wait patiently

Figure.141

(1) Generate report: The user can set the report items in the "Report Items" on

86
 the right side. After resetting each time, click "Generate" to regenerate a
consolidated report again (the default report contains all report items
when entering):

Figure.142

(2) Print: it will use the computer's print settings to print the generated report

W. Menu bar functions description: Database

• Experiment library

The user can view experiment file library, and at the same time sort, search,
open, import, and export them

87
 Figure.143

i. Sorting: Same as the sorting operation

ii. Search: Same as the search operation
iii. Delete: delete the selected experiment file. The user needs to confirm again
before deleting
Figure.144

iv. Open: Same as the search operation

v. Import: Same as the import operation
vi. Export: Select one or use the shift + ctrl keys of the keyboard to select multiple
files. Click export, and select the target folder to export the data to the target
directory

88
 Figure.145

If the selected file(s) to be exported already exist(s) in the target directory, the
user can skip or overwrite

• Template library

The user can view template file library, and at the same time sort, search,
open, import, export and delete them

Figure.146

(1) Sorting: same as the sorting operation

(2) Search: same as the search operation

(3) Open: same as the search operation

(4) Import: same as the import operation

(5) Export: select one or use the shift + ctrl keys of the keyboard to select

89
 multiple files, click “Export”, and select the target folder to export the data

Figure.147

If the selected file(s) to be exported already exist(s) in the target folder, the
user can skip or overwrite

Figure.148

Delete: delete the selected template file. The user needs to confirm again
before deleting

Figure.149

X. Menu bar functions description: Help

• Documentation

The user can consult the software and the operating manual matched with

90
 the instrument. Before using the instrument, the novice can view the
operation process in the "Documentation". This process can help the user
quickly become familiar with the experimental operation

• About

The user can view information such as instrument model, serial number, PC
full version and PC release version

Figure.150

91
Maintenance

A. Instrument cleaning

• Cleaning of instrument shell

If the surface of the instrument is splashed with contaminants, please use a

dust-free cloth dipped in 75% ethanol to wipe the surface of the instrument,
and wipe back and forth twice each time

• Cleaning the block wells of instrument

If liquid or other contaminants enter the block well , please use a medical
cotton swab soaked in 75% ethanol and put it into the block well and gently
rotate it 5-10 times, cleaning 3 times each time.

B. Routine maintenance

i. Check regularly whether the operating environment and placement of the

instrument comply with the requirement. Run the experiment regularly to
avoid the instrument not being turnedon for a long time.
ii. Clean regularly according to the method

92
Replacement

• When the fuse is damaged, the user can replace the fuse as follows

i. As shown in Figure use a flat-blade screwdriver (or other objects) to insert

into the disassembly slot. Move in the direction of arrow①, and remove the
fuse holder
ii. Remove the fuse in the direction of arrow ②
iii. In the direction of arrow ③, press the new fuse into the slot
iv. In the direction of arrow④, press the fuse holder into the power holder to
complete the installation.

93
 Troubleshooting

Alarm
Cause analysis Solution
signal/instrument
failure
Error01: In-out motor
Please contact the
abnormal. Please Instrument failure.
supplier or
contact after-sales
manufacturer.
personnel for overhaul.
Error02: Up-down
motor abnormal. Please contact the
Instrument failure. supplier or
Please contact
after-sales manufacturer.
personnel for
overhaul.
Error03: X axis motor
Please contact the
abnormal. Please Instrument failure.
supplier or
contact after-sales
manufacturer.
personnel for overhaul.
Error04: Y axis motor
Please contact the
abnormal. Please Instrument failure.
supplier or
contact after-sales
manufacturer.
personnel for overhaul.
Error05: Circular
channel motor Please contact the
Instrument failure.
abnormal. Please supplier or
contact manufacturer.
after-sales personnel for
overhaul.
1. Please contact the
1. Instrument failure.
supplier or manufacturer.
Error06: Fluorescence 2. Fluorescence signal is
2. Check whether the
detection abnormal. too low, no test tube or
plate placement is
empty tube may be
consistent with the
placed.
plate layout in the
program.

94
Error07: Module
temperature sensor Please contact the
Instrument failure. supplier or
short/open circuit.
Pleasecontact after- manufacturer.
sales personnel for
overhaul.
Error08: Module
heating function Please contact the
Instrument failure.
abnormal. Please supplier or
contact after-sales manufacturer.
personnel for overhaul.
Error09: Module
cooling function Please contact the
Instrument failure. supplier or
abnormal. Please
contact the after-sales manufacturer.
personnel
for overhaul.
Error10: Hot-lid
Please contact the
temperature sensor Instrument failure.
supplier or
short/open circuit.
manufacturer.
Please contact after-
sales personnel for
Error11: Hot-lid heating
function Abnormal.
Instrument failure. Please contact the
Please contact after-
sales personnel for supplier or
overhaul. manufacturer.
Error12: Radiator
temperature sensor
Instrument failure. Please contact the
short/open circuit.
Please contact after- supplier or
sales personnel for manufacturer.
overhaul.
Error13: The heat
dissipation function of
Instrument failure. Please contact the
the radiator is
abnormal. Please supplier or
contact after-sales manufacturer.
personnel for
overhaul.

95
Error14: |The module is The block is pulled out
Check the module
unplugged, and the during runtime.
status, relocate the
experimental
sample and restart
data is invalid.
the experiment
Error15: The firmware 1. The power is cut off 1. Power on and start
upgrade failed. Please 2. The PC is disconnected online again.
upgrade again! from the instrument 2. Reconnect.
terminal.
Error16: 1. Reconnect.
1. Communication data
Communication failure 2. Please contact the
error.
of Firmware! supplier or manufacturer.
2. Instrument failure.
Error17: Instrument Instrument on-line
Reconnect.
disconnected! disconnection.

96