Biology Compiled Labs
Biology Compiled Labs
5 Osmosis - PD Osmosis Your mother has a shriveled carrot that she wants P D
to become turgid (firm) again. She does not remember if
the carrot should be placed in brine or fresh water. Plan and
design an experiment to assist your mother so that she can
get to know whether it is fresh water or brine that she
should use if she wants the carrot to become turgid again.
7 Nutrition and disease It was observed that some tomato plants leaves, growing in PD
a pot started to have a purple tinge to them. Plan and design
an experiment to determine the nutrient lacking in the plant
that is causing it to turn purple.
8 Nutrition To test food samples for the presence of starch, protein, ORR / AI
fats, reducing and non-reducing sugar
9 P/D Proposal PD
14 Implementation A/I
18 Irritability While doing a nature walk around the school compound a P/D
group of grade 10 students noticed that most of the
invertebrates seen were found under a rock or a dead log.
Plan and design an experiment to determine the factor that
causes these organisms to be found under the rock.
Date:
Topic:
Aim
Apparatus/Materials:
Procedure :
Observations
Results:
Discussion:
Conclusion
It is preferred that students write with only one colour ink when writing up the SBAs, i.e. blue or
black ink.
SBAs must be written in the past tense, except for Planning and Designing (P&D) which must be
written in present tense.
ALL table(s) and drawing(s) must have a title. Remember drawing(s) to the left and labeling to
the right.
Hypothesis: Your hypothesis is like a good guess or prediction of what you expect to find from carrying out
your experiment. Even though your hypothesis is a good guess, it is stated as a definite
prediction e.g. X 'will' or 'will not' happen. In addition, a well-developed hypothesis should
begin with a short phrase which links back to the theoretical basis for the experiment.
Aim: An aim identifies the purpose of the investigation. It is a straightforward expression of what the
researcher is trying to find out from conducting an investigation. The aim typically involves the
word “investigate” or “investigation”.
Apparatus & Materials: A listing of all equipment, and chemicals needed to carry out the experiment
Diagram (if applicable)
Method: This section of the report gives a detailed account of the procedure that will be followed in
completing the experiment discussed in the report. This is very important as it gives the reader a
clear understanding of the experiment, and also serves as a set of instructions for anyone desiring
to replicate the study in the future. For a planning and design lab the method is written in
PRESENT TENSE.
Variables
The things that are changing in an experiment are called variables. A variable is any factor, trait, or condition
that can exist in differing amounts or types. An experiment usually has three kinds of variables: controlled,
manipulating and responding.
· Controlled – A control variable is the one element that is not changed throughout an experiment, because
its unchanging state allows the relationship between the other variables being tested to be better understood.
· Responding – The responding (or Dependent) Variable is the variable that changes as a result of the
changes in the manipulated variable.
Expected results – This must be clearly stated. This can be in the form of a table or graph. Often times it is a
short paragraph describing what you expect to happen.
Precautions / limitations – Any shortcomings of the design which may become sources of error.
Experiment: # 1
Date:
Skills: Observation Recording and Reporting and Measurement and Manipulation (O/R/R and M/M)
Aim: To investigate the distribution of various plant species in a particular terrestrial habitat on the school’s
compound.
Method:
1. Place Students in groups.
2. Identify a particular area of the school ground and investigate the different plant species.
3. Throw the quadrat randomly within the designated area.
4. Repeat step 3, nine (9) more times.
5. Record the data in a table and use it to find out the total number of plant species, frequency and the
species cover.
Calculations:
Sum of plant species in quadrats 1 – 10 = Add all the number of organism of percentage in all 10 quadrats
Species density = Total found in quadrats / number of quadrats (Used only when you have the actual number of
organism)
Species cover = Total found in quadrats / number of quadrats (Used only when you have the percentage of
organism)
Species frequency = % of quadrats in which a particular species was found
Total area sampled = size of quadrat × # of throws
Discussion:
1. What is ecology? What is an ecosystem? (ensure the relation between both terms are established and site
examples in each case.)
2. Outline the main sampling methods used in ecological study.
3. (a) Describe the sampling method used in the experiment (include why this sampling method is
appropriate).
4. Discuss results
(b) State the percentage cover of plant species found in the particular habitat.
Criteria Marks
Organization and conciseness of report/format:
Table
i. Table title above table in all caps /1
Total /10
Experiment: # 2
Date:
Aim: To identify feeding relationship among organisms from an area on the school compound
Apparatus/:
Diagram:
Method:
1. Observe and record ten organism found within the school environs
2. Construct a table showing the different feeding source of each organism.
3. Construct two food chains and one food web from the information in the table.
4. For the constructed food web indicate the trophic level of the different organism
5. Indicate if any special feeding relationships are observed.
Discussion:
1. What is a food chain, what is a food web
2. What does a food chain and food web show
3. The effect on the given food web if an organism becomes extinct or is harmed
Conclusion:
Criteria Marks
Organization and conciseness of report/format:
i. logical sequence of report 1
ii. all sections named correctly 1
Method clearly described 1
Language and expression: correct tense; passive voice and few 1
or no grammatical errors
Details of data present:
i. accuracy of recording observation 2
ii. use of table to represent data 1
iii. Columns correctly labelled 1
iv. correct food web 2
Total 10
Experiment # 3
Date:
Topic: Cells
Skill Assessed: Drawing
Aim: To draw, label and annotate an onion cell as seen under a light microscope.
Apparatus/ Materials: onion, blank sheet, ruler, pencil, light microscope
General guidelines
Drawing labs do not need a complete write – up. A blank page must be used.
Points
Clarity
Accuracy
Labelling
Title
- View/orientation stated 1
- Written in capitals, underlined and placed below the drawing 1
Magnification
/10
Experiment # 4
Date:
Topic: Osmosis
Skill Assessed: Analysis and Interpretation (ORR) and measurement and manipulation (MM)
Aim: To investigate the effects of osmosis on potato cells in salt solution of varied concentration.
Apparatus: measuring cylinder, ruler, scalpel, three petri dishes, spoon, stopwatch
Materials: label tape, salt solutions 5% and 10 %, pure water, blotting paper
Diagram:
Method:
1. Cut six strips of potato into exact lengths of 3cm.
2. Label three petri dishes A, B and C respectively.
3. In dish A add 20 cm3 of pure water.
4. In dish B add 20 cm3 of 5% salt solution.
5. In dish C add 20 cm3 of 50% salt solution.
6. Add TWO strips each to each petri dish and start timer for 15minutes.
7. After 30 minutes, remove strips and place on blotting paper.
8. Measure and record the new lengths of each strip in a results table.
Observation / result:
TABLE SHOWING THE EFFECT OF OSMOSIS ON POTATO CELLS PLACED IN WATER AND
SALT SOLUTIONS
Petri dish Original New length of Average Appearance of Texture of
length of each each strip change in strips strips
strip (cm) (cm) length of
strips/ cm
1 2 1 2
A (control)
B ( 5% salt
solution)
C (50% salt
solution)
Criteria Marks
Measuring
Proper use of ruler 1
Measuring cylinder on flat surface 1
Reading below meniscus 1
Eye level 1
Accurate volume 1
Total 10
Criteria Marks
Background information
Defining osmosis 1
Why use potato 1
Explanations of the results for the differences of plant 6
tissue in varying
A ( water)
B ( 5% salt solution)
Experiment: # 5
Date:
Topic: Osmosis
Problem Statement: Your mother has a shriveled carrot that she wants to become turgid (firm) again. She does
not remember if the carrot should be placed in brine or fresh water. Plan and design an
experiment to assist your mother so that she can get to know whether it is fresh water or
brine that she should use if she wants the carrot to become turgid again.
Hypothesis:
Aim:
Apparatus & Materials:
Diagram (if applicable)
Method
Variables
Controlled
Manipulated
Responding
Expected results
Discussion of expected results
Assumptions
Precautions / limitations
Criteria Marks
hypothesis
Stated clearly and based on problem statement 1
Testable
1
Aim related to hypothesis 1
Materials/Apparatus-appropriate 1
Method
Suitable 1
Manipulated or responding variable included. 1
Date:
Topic: Photosynthesis
Apparatus: Heat source, wire gauze, large beaker, boiling tube, dropper, test tube holder, tongs, tile / plate
Observation:
Note the following:
Texture of leaf before and after being boiled.
Colour of alcohol before leaf was placed in it and colour seen after the 15 minutes
Colour of leaf after being removed from alcohol
Colour seen upon addition of iodine to leaf.
Criteria Marks
Follows instruction (sequential order of steps from 2
method)
Uses laboratory equipment properly and
materials economically.
i. correct quantity of alcohol used. 1
ii. correct quantity of iodine used. 1
Handles apparatus and materials with care
i. monitor water bath so that alcohol does not 1
spill over.
Experiment: # 7
Date:
Topic:
Problem Statement: It was observed that some tomato plants leaves, growing in a pot started to have a purple
tinge to them. Plan and design an experiment to determine the nutrient lacking in the plant that is causing it to
turn purple.
Hypothesis:
Aim:
Apparatus & Materials:
Diagram (if applicable)
Method
Variables
Controlled
Manipulated
Responding
Expected results
Precautions / limitations
Criteria Marks
hypothesis
Stated clearly and based on problem statement 1
Testable
1
Aim related to hypothesis 1
Materials/Apparatus-appropriate 1
Method
Suitable 1
Manipulated or responding variable included. 1
Experiment # 8
Date:
Topic: Nutrition
Skill Assessed: ORR / AI
Aim: To test food samples for the presence of starch, protein, fats, reducing and non-reducing sugar
Apparatus/ Materials: heat source, beakers, test tubes, boiling tubes, droppers, iodine, sodium hydroxide,
copper (II) sulphate, ethanol, benedicts solution, Sodium Hydrogen Carbonate, Hydrochloric acid, water,
Unknown food samples X,Y and Z.
Method:
1. To sample X add a few drops of iodine and record observation.
2. Measure 2cm3 of sample X and pour into a test tube. To this tube Pour 1cm3 of sodium hydroxide
solution following which Add 2 drops of 1% copper (II) sulphate solution, drop by drop shaking the tube
after each drop. Record observation
3. Measure 2cm3 of sample X and pour into a test tube. To this add 2cm3 of ethanol and 2cm3 water. Shake
and observe.
4. Measure 2cm3 of sample X and pour into a boiling tube. To this sample add 2cm3 of Benedicts’ solution.
Place tube into a water bath and boil for 5mins. Record observation
5. Measure 2cm3 of sample X and pour into a boiling tube. To this sample add 2cm3 of HCl and place tube
into a water bath and boil for 5mins. Remove from water bath and add NaHCO3. Add 2cm3 Benedicts’
solution place in water bath. Record observation.
6. Repeat steps 1 – 5 with sample Y and Z.
Results table.
Reducing sugar
Emulsion test
Biuret test
Starch test
SAMPLE
Z
Discussion
1. Relevant background information.
2. Identify which nutrient is present in the different food samples X,Y, Z explaining clearly how that was
determined.
3. Did any food sample contain more than one nutrient? Explain.
4. Importance of these nutrients to the body.
5. Precaution / limitation/ source of error.
Observation, recording and reporting
Criteria Marks
Observation
Colour changes before and after each test accurate 4
Recording
Table with appropriate title above written in capital letters 1
Headings present 1
Table enclosed 1
Reporting
Correct format 1
Few or no grammatical error 1
Conclusion stated 1
Total 10
Criteria Marks
-Background information: role of Nutrients & enzymes; general 2
action of enzymes (substrate and product formed from reaction)
-Explanations/interpretation of the colour changes obtained in the 4
results.
-Correctly identify the substance X,Y & Z
-Controlling and manipulating variable identified 2
-precaution/limitation/source of error 1
-Conclusion related to aim. 1
Total 10
Experiment: # 9
Date:
Part 1 – Implementation
Problem Statement: James and Jimmy were arguing about the presence of chlorophyll in all leaves and their
ability to photosynthesize. James suggested that chlorophyll is present only in green leaves and therefore are
able to carry out photosynthesis and as such plants that have coloured leaves have to depend on other parts of
the plant to carry out photosynthesis. Jimmy is however adamant that chlorophyll is present in all leaves despite
their colour and as such they are also able to photosynthesize. Suggest a hypothesis and plan and design an
experiment to investigate the suggested hypothesis.
Hypothesis:
Aim:
Apparatus & Materials:
Diagram (if applicable)
Method
Variables
Controlled
Manipulated
Responding
Expected results
Precautions / limitations
Criteria Marks
hypothesis
Stated clearly and based on problem statement 1
Testable
1
Aim related to hypothesis 1
Materials/Apparatus-appropriate 1
Method
Suitable 1
Manipulated or responding variable included. 1
Experiment # 10
Date:
Topic: Reproduction
Skill Assessed: Drawing
Aim: To draw, label and annotate the internal and external structure of a red pea
Apparatus/ Materials: red pea , blank sheet, ruler, pencil
General guidelines
Drawing labs do not need a complete write – up. A blank page must be used.
Points
Clarity
Accuracy
Title
- View/orientation stated 1
- Written in capitals, underlined and placed below the drawing 1
Magnification
/10
Experiment 11
Date:
Topic: Respiration
Method:
1. Draw a table to record the results to show the pulse rate at rest, jogging and running.
3. Allow Person A to sit quietly for one (1) minutes to be completely relaxed.
4. Count the number of pulses for one (1) minute and record in the table.
6. Immediately after, count the number of pulse for one minute and record.
8. Immediately after allow person A to run for two (2) minutes and afterwards count the number of
10. Take the pulse for one (1) minute and record the result as recovery one (R1).
11. Allow the person to rest again for one (1) minute.
12. Count the pulse for one (1) minute afterwards and record this result as the 2nd recovery (R2).
13. Repeat steps 11 and 12, but record the result as 3rd recovery (R3).
15. Plot a line graph using data present in the table for comparison of pulse rate at rest, jogging and
Individual A Individual B
Resting
Jogging
Running
Recovery
R1
R2
R3
Conclusion: How did the pulse rate change with the activities and why?
Skill Assessed: Observation, Recording and Reporting & Analysis and Interpretation
c. include equations; 1
2. Evaluating details
a. Explain the increase pulse beat means increase in oxygen intake and
carbon dioxide removal for respiration 2
b. Deduce that lactic acid is formed in anaerobic respiration 1
c. Deduce that panting means oxygen debt is being repaid 1
d. Deduce from data that the healthier individual has lower pulse rates overall _________
10
Experiment #: 12
Date:
Method:
2. Using the clear tape and forceps attach the cobalt chloride to the upper and lower surface to one leaf on
each plant.
Results
Background information
Compare plant A with plant B indicating if the results are similar or different and explain why or why not.
Conclusion
Criteria Marks
Accuracy of observations/recordings: major changes 2
noted; comparison of appearance of cobalt chloride
paper on both surfaces of the leaf.
Experiment #: 13
Date:
Topic: Excretion
Skill Assessed: Drawing
Aim: To draw the internal structure of a mammalian kidney
Apparatus/ Materials: mammalian kidney, blank sheet, ruler, pencil
General guidelines
Drawing labs do not need a complete write – up. A blank page must be used.
Points
Clarity
Accuracy
Title
- View/orientation stated 1
- Written in capitals, underlined and placed below the drawing 1
Magnification
/10
Experiment #14
Date:
Topic: Implementation
Skill Assessed: AI
Materials:
Experiment #15
Date:
Aim: To investigate the effects of temperature on the rate of germination red peas.
Material/Apparatus: Newspaper, 3 beakers, a laboratory thermometer, 6 read peas, 75ml of tap water, 3
suitable environmental condition, measuring cylinder, cm ruler
Method:
Discussion:
What is germination?
Factors that affect germination.
Discuss your results for each condition
Deduce which condition is the best for seeds to germinate
Precaution, limitation, source of error
Reflection
Conclusion:
NOTE
Any other threes can be decided on to represent various temperatures such as an air-conditioned room, in the
hot sun.
Any suitable container may be used such as juice boxes, cut off water bottles etc.
MARK SCHEME
Marks
Recording
Table enclosed-----------------------------------------------------------------------1
Descriptive observation-------------------------------------------------------------1
Observation
Reporting
Aim------------------------------------------------------------------------------------1
Format---------------------------------------------------------------------------------1
Reflection-----------------------------------------------------------------------------1
Conclusion----------------------------------------------------------------------------1
Total 10 Marks
Experiment # 16
Date:
AIM: To determine the variation of height and tongue rolling among 20 students.
METHOD:
Select twenty individuals of the same sex and age and record the presence or absence of
The ability to roll their tongue and also their height.
Using the data from the continuous characteristics construct a histogram.
Using the data from the discontinuous characteristics construct a bar chart.
RESULT
Title: ____________________________________________________________________________________
Discussion
Background information
Guided Questions
Conclusion
Background information--------------------------------------------------------------------2
Reason for possible genotype of the parents for individuals with dominant trait----1
Limitation--------------------------------------------------------------------------------------1
CONCLUSION-------------------------------------------------------------------------------2
Total 10 Marks
Conversion of units 2
(centimeter to metre)
Date:
Topic: Genetics
Method
2. Count and place 25 black beads and 25 white beads into a 250ml beaker. Mix thoroughly.
4. Working in pairs blindfold one individual and allow them to pick a bead from each container.
6. If one bead is white and the other black place in the beaker labelled B.
1
2
10
Discussion
Background information
Definition of chromosome
Explanation of results
Interpretation of data received – (ratio of boys to girls) and was the data received as was expected.
Conclusion
Criteria Marks
Background Information relating what each of the following
represent in reality:
i. Black beads 1
ii. Black and white 1
iii. Beaker 1
Summarizing data:
Determining the ratio of boys to girls from data 1
Comparison of results of genetic diagram with the experiment 2
Evaluating Data:
i. From the table determine the ratio of boys to 1
girls in the ten (10) offspring
ii. one precaution 1
iii. Limitations of the experiment when compared to reality. 1
Date:
Topic: Irritability
Skill Assessed: PD
Problem Statement: While doing a nature walk around the school compound a group of grade 10 students
noticed that most of the invertebrates seen were found under a rock or a dead log.
Plan and design an experiment to determine the factor that causes these organisms to be found under the rock.
Hypothesis:
Aim:
Apparatus & Materials:
Diagram (if applicable)
Method
Variables
Controlled
Manipulated
Responding
Expected results
Precautions / limitations
Criteria Marks
hypothesis
Stated clearly and based on problem statement 1
Testable
1
Aim related to hypothesis 1
Materials/Apparatus-appropriate 1
Method
Suitable 1
Manipulated or responding variable included. 1