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Validaton Summery

The document describes results from validation of a method for determining free fatty acids in edible oils. It includes tables showing trial results and calculations from three analysts and performs an analysis of variance to compare their results. The method was validated according to standard procedures and the results met acceptance criteria.

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12 views3 pages

Validaton Summery

The document describes results from validation of a method for determining free fatty acids in edible oils. It includes tables showing trial results and calculations from three analysts and performs an analysis of variance to compare their results. The method was validated according to standard procedures and the results met acceptance criteria.

Uploaded by

asresmekonne13
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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LABORATORY: physicochemical Testing Laboratory

METHOD REFERENCE&DESCRIPTION: ESISO660:2009: Determination of free


fatty acid
NOTE: Indicate the degree of applicability of each of the validation activities in accordance with
The guidance provided in the Test Method procedure. Where an activity is applicable provide a
Summary of the outcome of the evaluation/study and cross-reference the supporting raw data.

1.Introduction
The principle of validation of quantitative analytical procedures is widely spread today in all
domains of activities where measures are made.This
validationreportpresentsresultsformoistureandvolatilematterinedibleoil
Asres.m

Trial no. weight(g) viKOH(ml) vfKOH(ml) FF%


1 20.0179 19.0 19.6 0.08408974
2 20.0229 19.6 20.1 0.07005728
3 20.0176 20.1 20.6 0.07007583
4 20.0113 20.6 21.1 0.07009789
5 20.0029 21.1 21.6 0.07012733
6 20.0258 22.4 23 0.08405657
7 20.0333 23 23.5 0.07002092
8 20.0258 23.5 24 0.07004714
9 20.0018 24 24.5 0.07013119
10 20.0205 24.5 25 0.07006568
Mean 0.07287696
SD 0.00590102
%RSD 8.09723279

Abie.G

Trial no. weight(g) viKOH(ml) vfKOH(ml) FF%


1 20.02 25.8 26.3 0.07006743
2 20.00 26.3 26.8 0.0701375
3 20.04 26.8 27.3 0.0699975
4 20.02 27.3 27.8 0.07006743
5 20.02 27.8 28.3 0.07006743
6 20.02 28.3 28.8 0.07006743
7 20.03 28.8 29.3 0.07003245
8 20.01 29.3 29.8 0.07010245
9 20.00 29.8 30.3 0.0701375
10 20.00 30.3 30.8 0.0701375
Mean 0.07008146
SD 4.7257E-05
%RSD 0.06743207

Birhanu .A

Trial no. weight(g) viKOH(ml) vfKOH(ml) FF%


1 20.0125 31.8 32.3 0.07009369
2 20.0173 32.3 32.8 0.07007688
3 20.0007 32.8 33.3 0.07013505
4 20.0003 33.3 33.8 0.07013645
5 20.0037 33.8 34.3 0.07012453
6 20.009 35 35.5 0.07010595
7 20.0059 35.5 36 0.07011682
8 20.005 36.1 36.6 0.07011997
9 20.0036 36.6 37.1 0.07012488
10 20.0057 37.1 37.6 0.07011752
Mean 0.07011517
SD 1.8478E-05
%RSD 0.02635396

1 0.08408974 0.070067433 0.070093691


2 0.070057284 0.0701375 0.070076883
3 0.070075833 0.069997505 0.070135045
4 0.070097895 0.070067433 0.070136448
5 0.070127332 0.070067433 0.070124527
6 0.084056567 0.070067433 0.070105952
7 0.070020915 0.07003245 0.070116816
8 0.070047139 0.070102449 0.07011997
9 0.070131188 0.0701375 0.070124878
10 0.070065683 0.0701375 0.070117517
Xmin. 0.070020915 0.069997505 0.070076883
Xmax. 0.08408974 0.0701375 0.070136448
Xsusp. 0.08408974 0.069997505 0.070136448
/Xsus.- 3.31727E-05 3.4945E-05 1.40268E-06
Xsus±1/
Range 0.014068825 0.000139995 5.95645E-05
Qcal. 0.002357884 0.249616111 0.02354894
Qcrit(0.05) 0.466 0.466 0.466
outlayer no outlarer no outlarer no outlarer
mean 0.072876958 0.070081463 0.070115173
SD 0.005901017 4.72575E-05 1.84781E-05
%RSD 8.097232787 0.067432291 0.026353965
Grand mean 0.071024531
SDP 0.001988918
Anova: Single Factor

SUMMARY
Varianc
Groups Count Sum Average e
0.07287 3.48E-
Asres.M 10 0.72877 7 05
0.70081 0.07008 2.23E-
Abie.G 10 5 1 09
0.70115 0.07011 3.41E-
Birhanu.A 10 2 5 10

ANOVA
Source of
Variation SS df MS F P-value F crit
Between 5.15E- 2.57E- 2.21731 0.12835 3.35413
Groups 05 2 05 1 1 1
0.00031 1.16E-
Within Groups 3 27 05

0.00036
Total 5 29

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