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UV-Vis Note

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16 views

UV-Vis Note

Uploaded by

knowledge updates
Copyright
© © All Rights Reserved
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UV-Visible Spectroscopy

Write down the basic principle of UV-Visible Spectroscopy:


The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet
light or visible light by chemical compounds, which results in the production of
distinct spectra. Spectroscopy is based on the interaction between light and matter.
When the matter absorbs the light, it undergoes excitation and de-excitation,
resulting in the production of a spectrum.

When matter absorbs ultraviolet radiation, the electrons present in it undergo


excitation. This causes them to jump from a ground state to an excited. It is important
to note that the difference in the energies of the ground state and the excited state of
the electron is always equal to the amount of ultraviolet radiation or visible radiation
absorbed by it.
Picture of UV-Vis Spectrophotometer

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