Agilent 7100 Capillary

Electrophoresis System
Highest sensitivity. Highest productivity. Minimal footprint.

Capillary Electrophoresis (CE) offers

fast separations with exceptional effi-
ciency and resolution for analytical
challenges that often can only be met
with difficulty by LC. Used in stand-
alone mode, as the separations com-
ponent of a CE/MS, or as a comple-
mentary, orthogonal technology to LC,
the Agilent 7100 CE system brings
Features
unprecedented HPLC-like sensitivity
• Most sensitive CE system on the market
to a wide range of analytical
• Replenishment system allows high throughput and unattended operation
challenges. In addition, CE offers the
• Easy maintenance and automated instrument diagnostics
advantage that several separation • Plug & play connectivity to all Agilent MS systems
modes can be run on a single instru- • Easy method setup data analysis
ment. This makes CE a very versatile • Regulatory compliance tools
technique for a broad range of appli- • Comprehensive offering of accessories and consumables
cations and separation challenges. • Partner solutions for LIF and electrochemical detection

Application Areas Benefits

CE is used to separate, identify, quantify • Best in class analytical performance
and fractionate compounds in: • Broadest selection of detectors in the industry
• Environmental analysis • Legendary Agilent reliability
• Food safety • Reverse-compatible with the Agilent HPCE
• Increased uptime
• Forensics
• Flexible separation modes including capillary electrochromatography
• Pharmaceutical discovery, • CE-MS support from the leading vendor with the longest CE-MS experience
development, and QA/QC • Broad application coverage
• Life Sciences • Complete solution for charged sample analysis
Specifications Agilent 7100 Capillary Electrophoresis System

Pressure system Raw data channels

Programmable with –100 to +100 mbar on inlet Detector signals and spectrum, voltage, current, leak current,
Flushing with 1 bar or with high pressure 2–12 bar power, cassette temperature, pressure, lamp voltage and detector
Vial pressurization with high pressure 2–12 bar on inlet temperature
and/or outlet
Diagnostic functions
Injection modes RFID tag for lamp, early maintenance feedback, supported by
Self correcting injection system with injection from inlet or outlet
Agilent Lab Advisor software with integrated diagnosis test suite
Programmable range: up to 10,000 seconds
Pressure: –100 to +100 mbar Safety features
Electrokinetic: –30 to +30 kV Current leak detection: low current limit
Liquid leak sensor
Autosampler/ fraction collector
Safety sensors at door and cover disabling high voltage
50-position carousel
Vial sensor
All vials are randomly accessible from inlet or outlet end
of capillary. Environment
Temperature control with external waterbath with vial Temperature: 5–40 °C
temperature from 10–40 °C. Humidity: up to 80 % rel. humidity at 31 °C (non-condensing)
(Non-condensing conditions, minimum waterbath
System control
temperature +1 °C).
Operating with graphical user interface under Windows XP (SP3)
Replenishment Time programmable parameters: voltage, current, power,
Satellite station for refilling of inlet or outlet buffer vials with polarity, pressure, inlet and outlet vial, capillary temperature,
fresh buffer for automatic, continuous operation. Selectable pre and post-run conditioning with pressure and/or voltage,
buffer levelling. Replenishment, fraction collection**
Vials CE specific software
100 µL (polypropylene or glas) with resealing snap caps Mobility report, time corrected areas, pI calibration and bio
1 mL (polypropylene) with resealing snap caps polymer size calibration.
2 mL (glas) with resealing snap caps
Physical Specifications
Capillary cassette
High-speed forced-air cooler with Peltier element Dimensions Interfaces
Temperature range: 10 °C below ambient, up to 60 °C Width: 35 cm LAN, CAN, RS232, remote
(min. 10 °C cassette temperature) Height: 59 cm control, analog in (1V, 20 bit,
Minimum total capillary length: 33 cm Depth: 51 cm integrated A/D converter),
Capillary compatibility 365 µm o.d. Weight: 35 kg analog out
Detector Power requirements Electrophoresis power
Real time UV-Visible diode-array detector (190–600 nm) Line voltage: 100-240 V, Voltage range: setable
Temperature controlled max. 300 W 0 to ± 30 kV supply
Wavelength accuracy: 1 nm Line frequency: 50-60 Hz Current: setable 0 - 300 µA
Response time: 0.025 to 10 s Power: setable 0 - 6 W operation
Light source: prealigned deuterium or high brightness lamp under constant voltage, current
Signals: up to eight signals simultaneously, full spectral acquisition or power
with Agilent ChemStation
Sensitivity: 1 µM 4-hydroxy-aceto-phenon injected at * typical value
50 mbar • 5 sec, 3 x 50 µm bubble cell capillary, **upgradeable
signal/noise >6* (50 mM borate buffer pH 9.3, 25 kV)
Baseline noise: <20 µAU (at 2 sec response time)*
Linear dynamic range: 1x104 (3 x 50 µm bubble cell capillary)*
www.agilent.com/chem/ce

© Agilent Technologies Inc., 2009

Published May 1, 2009
Publication Number 5990-3962EN