PhysioEx Lab Report
Exercise 11: Blood Analysis
Activity 3: Hemoglobin Determination
Name: MERU ANUSHREE ASHWINKUMAR
Date: 29 July 2023
Session ID: session-d6a37a15-cc8b-25d1-0244-b263b22e03f7
Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
1 A protein found in red blood cells, __________, is necessary for the transport of oxygen
from the lungs to the cells of the body.
You correctly answered: hemoglobin (Hb).
2 Anemia results when
You correctly answered: There is inadequate hemoglobin in the red blood cells, and
insufficient oxygen is carried in the blood.
3 How much hemoglobin does normal adult human blood contain?
You correctly answered: 12–18 g/100 ml.
Experiment Results
Predict Question
1 Predict Question: How will the hemoglobin levels for the female Olympic athlete
(sample 5) compare with the hemoglobin levels for the healthy female (sample 2)?
Your answer: The hemoglobin levels for the female Olympic athlete will be greater than
those for the healthy female.
Stop & Think Question
1 Why is the average hematocrit higher in males than in females?
You correctly answered: Higher testosterone levels in males promotes more RBC
production.
Experiment Data
Blood sample gm Hb per 100 ml Hematocrit (PCV) Ratio of PCV to Hb
Blood sample gm Hb per 100 ml Hematocrit (PCV) Ratio of PCV to Hb
blood
1 16 48 3:1
2 14 44 3.14:1
3 8 40 5:1
4 20 60 3:1
5 22 60 2.73:1
Sample 1: healthy male
Sample 2: healthy female
Sample 3: female with iron-deficiency amenia
Sample 4: male with polycythemia
Sample 5: female Olympic athlete
Post-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
1 To determine the amount of hemoglobin in a blood sample
You correctly answered: All of these answers are correct.
2 Which of the following diseases is known to increase hemoglobin levels in an
individual?
You correctly answered: polycythemia.
3 Which of the following is known to decrease hemoglobin levels in an individual?
You correctly answered: cirrhosis of the liver.
Review Sheet Results
1 Is the male with polycythemia (sample 4) deficient in hemoglobin? Why?
Your answer:
No it is within range because polycethemia increases hemocrit.
2 How did the hemoglobin levels for the female Olympic athlete (sample 5) compare with
the hemoglobin levels for the healthy female (sample 2)? Is either person deficient in
hemoglobin? How well did the results compare with your prediction?
Your answer:
The female athlete had higher hemoglobin and both are within range. My hypothesis
was correct.
3 List conditions in which hemoglobin levels would be expected to decrease. Provide
reasons for the change when possible.
Your answer:
Hemoglobin would increase in high altitudes. This is because hemocrit would increase
because of the lack of oxygen density within the atmosphere.
4 List conditions in which hemoglobin levels would be expected to increase. Provide
reasons for the change when possible.
Your answer:
If someone started consistently running everyday, their hemoglobin would increase
because
their hemocrit would increase. This is because red blood cells would need to be
produced faster to maintain oxygen supply for aerobic activity.
5 Describe the ratio of hematocrit to hemoglobin for the healthy male (sample 1) and
female (sample 2). (A normal ratio of hematocrit to grams of hemoglobin is
approximately 3:1.) Discuss any differences between the two individuals.
Your answer:
There ratios are both within the healthy range. The male has higher RBC's which leads
to a
higher disparity of hemoglobins.
6 Describe the ratio of hematocrit to hemoglobin for the female with iron-deficiency
anemia (sample 3) and the female Olympic athlete (sample 5). (A normal ratio of
hematocrit to grams of hemoglobin is approximately 3:1.) Discuss any differences
between the two individuals.
Your answer:
The female with the iron deficiency is not able to create enough hemoglobin in
proportion to
her red blood cells count.
